Role of Ribes khorassanicum in the biosynthesis of AgNPs and their antibacterial properties

Silver nanoparticles (AgNPs) have been biosynthesised through the extracts of Ribes khorassanicum fruits, which served as the reducing agents and capping agents. Biosynthesised AgNPs have been found to be ultraviolet–visible (UV–vis) absorption spectra since they have displayed one surface plasmon resonance peak at 438 nm, attesting the formation of spherical NPs. These particles have been characterised by UV–vis, field‐emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy analysis. The formation of AgNPs at 1.0 mM concentration of AgNO₃ has resulted in NPs that contained mean diameters in a range of 20–40 nm. The green‐synthesised AgNPs have demonstrated high antibacterial effect against pathogenic bacteria (i.e. Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa). Biosynthesising metal NPs through plant extracts can serve as the facile and eco‐friendly alternative for chemical and/or physical methods that are utilised for large‐scale nanometal fabrication in various medical and industrial applications.Inspec keywords: X‐ray diffraction, X‐ray chemical analysis, nanofabrication, surface plasmon resonance, nanoparticles, antibacterial activity, microorganisms, scanning electron microscopy, silver, nanomedicine, visible spectra, ultraviolet spectra, transmission electron microscopy, Fourier transform infrared spectra, field emission scanning electron microscopy, biomedical materialsOther keywords: antibacterial properties, silver nanoparticles, reducing agents, capping agents, surface plasmon resonance peak, spherical NPs, field‐emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, transmission electron microscopy analysis, plant extracts, ultraviolet‐visible absorption spectra, Fourier transform infrared spectroscopy, antibacterial effect, Ribes khorassanicum fruits, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, surface plasmon resonance, AgNO₃ , Ag     

Fabrication and characterisation of silver nanoparticles using bract extract of Musa paradisiaca for its synergistic combating effect on phytopathogens,free radical scavenging activity,and catalytic efficiency

This work explores the rapid synthesis of silver nanoparticles (AgNPs) from Musa paradisiaca (M. paradisiaca) bract extract. The bio‐reduction of Ag⁺ ion was recorded using ultraviolet–visible spectroscopy by a surface plasmon resonance extinction peak with an absorbance at 420 nm. The phytoconstituents responsible for the reduction of AgNPs was probed using Fourier transform infrared spectroscopy. The X‐ray diffraction pattern confirmed the formation of crystalline AgNPs that were analogous to selected area electron diffraction patterns. Morphological studies showed that the obtained AgNPs were monodispersed with an average size of 15 nm. The biologically synthesised AgNPs showed higher obstruction against tested phytopathogens. The synthesised AgNPs exhibited higher inhibitory zone against fungal pathogen Alternaria alternata and bacterial pathogen Pseudomonas syringae. Free radical scavenging potential of AgNPs was investigated using 1,1‐diphenyl‐2‐picryl hydroxyl and 2,2‐azinobis (3‐ethylbenzothiazoline)‐6‐sulphonic acid assays which revealed that the synthesised AgNPs act as a potent radical scavenger. The catalytic efficiency of the synthesised AgNPs was investigated for azo dyes, methyl orange (MO), methylene blue (MB) and reduction of o‐nitrophenol to o‐aminophenol. The results portrayed that AgNPs act as an effective nanocatalyst to degrade MO to hydrazine derivatives, MB to leucomethylene blue, and o‐nitro phenol to o‐amino phenolInspec keywords: catalysis, dyes, electron diffraction, nanofabrication, silver, catalysts, surface plasmon resonance, reduction (chemical), free radicals, nanoparticles, transmission electron microscopy, nanobiotechnology, X‐ray diffraction, microorganisms, organic compounds, Fourier transform spectra, nanomedicine, visible spectra, antibacterial activity, infrared spectra, ultraviolet spectraOther keywords: silver nanoparticles, musa paradisiaca, synergistic combating effect, free radical scavenging activity, catalytic efficiency, M. paradisiaca, bio‐reduction, ultraviolet–visible spectroscopy, surface plasmon resonance extinction peak, Fourier transform infrared spectroscopy, X‐ray diffraction pattern, selected area electron diffraction patterns, radical scavenging potential, potent radical scavenger, size 420.0 nm, size 15.0 nm, Ag⁺      

Green biosynthesis,characterisation and antimicrobial activities of silver nanoparticles using fruit extract of Solanum viarum

Green synthesis of nanoparticles is considered an efficient method when compared with chemical and physical methods because of its bulk production, eco‐friendliness and low cost norms. The present study reports, for the first time, green synthesis of silver nanoparticles (AgNPs) at room temperature using Solanum viarum fruit extract. The visual appearance of brownish colour with an absorption band at 450 nm, as detected by ultraviolet‐visible spectrophotometer analysis, confirmed the formation of AgNPs. X‐ray diffraction confirmed the AgNPs to be crystalline with a face‐centred lattice. The transmission electron microscopy‐energy dispersive X‐ray spectroscopy image showed the AgNPs are poly‐dispersed and are mostly spherical and oval in shape with particle size ranging from 2 to 40 nm. Furthermore, Fourier transform‐infrared spectra of the synthesised AgNPs confirmed the presence of phytoconstituents as a capping agent. The antimicrobial activity study showed that the AgNPs exhibited high microbial activity against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus susp. aureus, Aspergillus niger, and Candida albicans. The highest antimicrobial activity of AgNPs synthesised by S. viarum fruit extract was observed in P. aeruginosa, S. aureus susp. aureus and C. albicans with zone of inhibition, 26.67 mm.Inspec keywords: nanomedicine, antibacterial activity, X‐ray chemical analysis, nanoparticles, transmission electron microscopy, particle size, infrared spectra, microorganisms, X‐ray diffraction, Fourier transform spectra, ultraviolet spectra, scanning electron microscopy, visible spectra, nanofabricationOther keywords: green biosynthesis, antimicrobial activities, silver nanoparticles, green synthesis, physical methods, study reports, solanum viarum fruit, ultraviolet‐visible spectrophotometer analysis, high microbial activity, highest antimicrobial activity, s. viarum fruit, transmission electron microscopy, energy dispersive X‐ray spectroscopy image     

Green synthesis of nanosilver‐decorated graphene oxide sheets

A green facile method has been successfully used for the synthesis of graphene oxide sheets decorated with silver nanoparticles (rGO/AgNPs), employing graphite oxide as a precursor of graphene oxide (GO), AgNO₃ as a precursor of Ag nanoparticles (AgNPs), and geranium (Pelargonium graveolens) extract as reducing agent. Synthesis was accomplished using the weight ratios 1:1 and 1:3 GO/Ag, respectively. The synthesised nanocomposites were characterised by scanning electron microscopy, transmission electron microscopy, atomic force microscopy, X‐ray diffraction, UV‐visible spectroscopy, Raman spectroscopy, energy dispersive X‐ray spectroscopy and thermogravimetric analysis. The results show a more uniform and homogeneous distribution of AgNPs on the surface of the GO sheets with the weight ratio 1:1 in comparison with the ratio 1:3. This eco‐friendly method provides a rGO/AgNPs nanocomposite with promising applications, such as surface enhanced Raman scattering, catalysis, biomedical material and antibacterial agent.Inspec keywords: silver, nanoparticles, graphene, nanocomposites, scanning electron microscopy, transmission electron microscopy, atomic force microscopy, X‐ray diffraction, ultraviolet spectra, visible spectra, X‐ray chemical analysis, surface enhanced Raman scattering, catalysis, nanofabricationOther keywords: antibacterial agent, biomedical material, catalysis, surface enhanced Raman scattering, rGO‐AgNP nanocomposite, eco‐friendly method, homogeneous distribution, thermogravimetric analysis, energy dispersive X‐ray spectroscopy, Raman spectroscopy, UV‐visible spectroscopy, X‐ray diffraction, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, nanocomposites, reducing agent, geranium, graphene oxide sheets, graphite oxide, silver nanoparticles, green facile method     

Antibacterial,anticancer and antioxidant potential of silver nanoparticles engineered using Trigonella foenum‐graecum seed extract

In this study, the authors report a simple and eco‐friendly method for the synthesis of silver nanoparticles (AgNPs) using Trigonella foenum‐graecum (TFG) seed extract. They explored several parameters dictating the biosynthesis of TFG‐AgNPs such as reaction time, temperature, concentration of AgNO₃, and TFG extract amount. Physicochemical characterisation of TFG‐AgNPs was done on dynamic light scattering (DLS), field emission electron microscopy, energy dispersive X‐ray spectroscopy, X‐ray diffraction and Fourier transform infrared spectroscopy. The size determination studies using DLS revealed of TFG‐AgNPs size between 95 and 110 nm. The antibacterial activity was studied against Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa and Staphylococcus aureus. The biosynthesised TFG‐AgNPs showed remarkable anticancer efficacy against skin cancer cell line, A431 and also exhibited significant antioxidant efficacy.Inspec keywords: antibacterial activity, cancer, biomedical materials, silver, nanofabrication, nanomedicine, nanoparticles, microorganisms, skin, cellular biophysics, biochemistry, light scattering, X‐ray chemical analysis, X‐ray diffraction, Fourier transform infrared spectra, particle sizeOther keywords: antibacterial potential, anticancer potential, antioxidant potential, silver nanoparticles, Trigonella foenum‐graecum seed extract, eco‐friendly method, biosynthesis, reaction time, AgNO₃ concentration, TFG extract amount, physicochemical characterisation, dynamic light scattering, field emission electron microscopy, energy dispersive X‐ray spectroscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, size determination, TFG‐AgNPs size, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, skin cancer cell line A431, Ag     

Green synthesis,characterisation and biological evaluation of plant‐based silver nanoparticles using Quercus semecarpifolia Smith aqueous leaf extract

The development of reliable and green methods for the fabrication of metallic nanoparticles (NPs) has many advantages in the field of nanotechnology. In this direction, the present work describes an eco‐friendly and cost‐effective protocol for the production of silver NPs (AgNPs) using an aqueous extract of Quercus semecarpifolia leaves. Different techniques were carried out for the characterisation of the synthesised AgNPs. The ultraviolet–visible spectroscopic analysis showed the highest absorbance peak at 430 nm. The particle size and structure were confirmed by scanning electron microscopy as well as transmission electron microscopy (TEM) analysis. From TEM imaging, it was revealed that the formed particles were spherical with an average size of 20–50 nm. The crystalline nature of the NPs was determined by X‐ray powder diffraction patterns. Thermogravimetry and differential thermal analysis were also evaluated by a temperature increment from 100 to 1000°C. Bio‐inspired synthesis of AgNPs was performed for their pharmacological evaluation in relation to the activities of the crude methanolic, n ‐hexane, chloroform, ethyl acetate, and aqueous extracts. Good cytotoxic activity was exhibited by the green‐synthesised AgNPs (77%). Furthermore, the AgNPs were found to exhibit significant antioxidant activity at 300 μg/ml (82%). The AgNPs also exhibited good phytotoxic potential (75%).Inspec keywords: scanning electron microscopy, toxicology, visible spectra, particle size, nanofabrication, nanomedicine, transmission electron microscopy, silver, ultraviolet spectra, differential thermal analysis, nanoparticles, X‐ray diffraction, botany, biochemistry, cellular biophysicsOther keywords: green synthesis, biological evaluation, plant‐based silver nanoparticles, reliable methods, metallic nanoparticles, eco‐friendly cost‐effective protocol, silver NPs, ultraviolet–visible spectroscopic analysis, highest absorbance peak, particle size, structure, transmission electron microscopy analysis, TEM imaging, crystalline nature, X‐ray powder diffraction patterns, differential thermal analysis, pharmacological evaluation, aqueous extracts, good cytotoxic activity, significant antioxidant activity, AgNPs exhibited good phytotoxic potential, bio‐inspired synthesis, Quercus semecarpifolia Smith aqueous leaf extract, scanning electron microscopy, thermogravimetry, crude methanolic, n‐hexane, chloroform, ethyl acetate, phytotoxic potential, haemagglutination activity, size 20.0 nm to 50.0 nm, wavelength 430.0 nm, temperature 100 degC to 1000 degC, Ag     

Biofabrication of silver nanoparticles using bacteria from mangrove swamp

The last decade has observed a rapid advancement in utilising biological system towards bioremediation of metal ions in the form of respective metal nanostructures or microstructures. The process may also be adopted for respective metal nanoparticle biofabrication. Among different biological methods, bacteria‐mediated method is gaining great attention for nanoparticle fabrication due to their eco‐friendly and cost‐effective process. In the present study, silver nanoparticle (AgNP) was synthesised via continuous biofabrication using Aeromonas veronii, isolated from swamp wetland of Sunderban, West Bengal, India. The biofabricated AgNP was further purified to remove non‐conjugated biomolecules using size exclusion chromatography, and the purified AgNPs were characterised using UV–visible spectroscopy, X‐ray diffraction, field emission scanning electron microscopy and transmission electron microscopy (TEM). Additionally, the presence of proteins as capping and stabilising agents was confirmed by the amide‐I and amide‐II peaks in the spectra obtained using attenuated total reflection Fourier transform infrared spectroscopy. The size of biofabricated AgNP was 10–20 nm, as observed using TEM. Additionally, biofabricated AgNP shows significant antibacterial potential against E. coli and S. aureus. Hence, biofabricated AgNP using Aeromonas veronii, which found resistant to a significant concentration of Ag ion, showed enhanced antimicrobial activity compared to commercially available AgNP.Inspec keywords: silver, nanoparticles, microorganisms, nanofabrication, purification, chromatography, ultraviolet spectra, visible spectra, X‐ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, proteins, molecular biophysics, biochemistry, molecular configurations, attenuated total reflection, Fourier transform infrared spectra, particle size, antibacterial activity, biomedical materials, nanomedicineOther keywords: capping agents, stabilising agents, amide‐I peaks, amide‐II peaks, attenuated total reflection Fourier transform infrared spectroscopy, antibacterial potential, E. coli, S. aureus, Aeromonas veronii, antimicrobial activity, size 10 nm to 20 nm, Ag, proteins, TEM, transmission electron microscopy, field emission scanning electron microscopy, X‐ray diffraction, UV‐visible spectroscopy, size exclusion chromatography, nonconjugated biomolecules, purification, swamp wetland, Aeromonas veronii, cost‐effective process, eco‐friendly, bacteria‐mediated method, biological methods, metal nanoparticle biofabrication, microstructures, metal nanostructures, metal ions, bioremediation, biological system, mangrove swamp, bacteria, silver nanoparticles     

Green synthesis of silver nanoparticles using Glaucium corniculatum (L.) Curtis extract and evaluation of its antibacterial activity

The metal nanoparticles, due to interesting features such as electrical, optical, chemical and magnetic properties, have been investigated repeatedly. Also, the mentioned nanoparticles have specific uses in terms of their antibacterial activity. The biosynthesis method is more appropriate than the chemical method for producing the nanoparticles because it does not need any special facilities; it is also economically affordable. In the current study, the silver nanoparticles (AgNPs) were obtained by using a very simple and low‐cost method via Glaucium corniculatum (L.) Curtis plant extract. The characteristics of the AgNPs were investigated using techniques including: X‐ray diffraction, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy. The SEM and TEM images showed that the nanoparticles had a spherical shape, and the mean diameter of them was 53.7 and 45 nm, respectively. The results of the disc diffusion test used for measuring the anti‐bacterial activity of the synthesised nanoparticles indicated that the formed nanoparticles possessed a suitable anti‐bacterial activity.Inspec keywords: silver, nanoparticles, antibacterial activity, nanomedicine, nanofabrication, X‐ray diffraction, transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectraOther keywords: green synthesis, silver nanoparticles, Glaucium corniculatum Curtis extract, antibacterial activity, metal nanoparticles, biosynthesis method, X‐ray diffraction, transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, SEM, TEM, spherical shape, disc diffusion test, Ag     

Biogenic synthesis,optimisation and antibacterial efficacy of extracellular silver nanoparticles using novel fungal isolate Aspergillus fumigatus MA

To eliminate the elaborate processes employed in other non‐biological‐based protocols and low cost production of silver nanoparticles (AgNPs), this study reports biogenic synthesis of AgNPs using silver salt precursor with aqueous extract of Aspergillus fumigates MA. Influence of silver precursor concentrations, concentration ratio of fungal extract and silver nitrate, contact time, reaction temperature and pH are evaluated to find their effects on AgNPs synthesis. Ultraviolet–visible spectra gave surface plasmon resonance at 420 nm for AgNPs. Fourier transform infrared spectroscopy and X‐ray diffraction techniques further confirmed the synthesis and crystalline nature of AgNPs, respectively. Transmission electron microscopy observed spherical shapes of synthesised AgNPs within the range of 3–20 nm. The AgNPs showed potent antimicrobial efficacy against various bacterial strains. Thus, the results of the current study indicate that optimisation process plays a pivotal role in the AgNPs synthesis and biogenic synthesised AgNPs might be used against bacterial pathogens; however, it necessitates clinical studies to find out their potential as antibacterial agents.Inspec keywords: nanoparticles, microorganisms, cellular biophysics, silver, antibacterial activity, pH, surface plasmon resonance, ultraviolet spectra, visible spectra, X‐ray diffraction, Fourier transform infrared spectra, optimisation, nanomedicine, nanofabricationOther keywords: biogenic synthesis, optimisation, antibacterial efficacy, extracellular silver nanoparticles, fungal isolate Aspergillus fumigatus MA, nonbiological‐based protocols, silver salt precursor, fungal extract, silver nitrate, pH, ultraviolet‐visible spectra, surface plasmon resonance, Fourier transform infrared spectroscopy, X‐ray diffraction, crystalline nature, transmission electron microscopy, spherical shapes, potent antimicrobial efficacy, bacterial strains, optimisation process, bacterial pathogens, antibacterial agents, wavelength 420 nm, size 3 nm to 20 nm, Ag     

Green synthesis of silver nanoparticles using Mentha pulegium and investigation of their antibacterial,antifungal and anticancer activity

A simple and eco‐friendly method for efficient synthesis of stable colloidal silver nanoparticles (AgNPs) using Mentha pulegium extracts is described. A series of reactions was conducted using different types and concentrations of plant extract as well as metal ions to optimize the reaction conditions. AgNPs were characterized by using UV–vis spectroscopy, transmission electron microscopy, atomic force microscopy, dynamic light scattering, zetasizer, energy‐dispersive X‐ray spectroscopy (EDAX) and Fourier transform infrared spectroscopy (FTIR). At the optimized conditions, plate shaped AgNPs with zeta potential value of ‐15.7 and plasmon absorption maximum at 450 nm were obtained using high concentration of aqueous extract. Efficient adsorption of organic compounds on the nanoparticles was confirmed by FTIR and EDAX. The biogenic AgNPs displayed promising antibacterial activity on Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes. The highest antibacterial activity of 25 µg mL‐1 was obtained for all the strains using aqueous extract synthesized AgNPs. The aqueous extract synthesised AgNPs also showed considerable antifungal activity against fluconazole resistant Candida albicans. The cytotoxicity assay revealed considerable anticancer activity of AgNPs on HeLa and MCF‐7 cancer cells. Overall results indicated high potential of M. pulegium extract to synthesis high quality AgNPs for biomedical applications.Inspec keywords: silver, nanoparticles, nanofabrication, botany, antibacterial activity, biomedical materials, nanomedicine, ultraviolet spectra, visible spectra, transmission electron microscopy, atomic force microscopy, X‐ray chemical analysis, Fourier transform infrared spectra, electrokinetic effects, microorganisms, cellular biophysics, cancerOther keywords: antibacterial activity, antifungal activity, anticancer activity, stable colloidal silver nanoparticle, Mentha pulegium, plant extract, UV‐visible spectroscopy, transmission electron microscopy, atomic force microscopy, DLS, zetasizer, energy‐dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy, methanolic extract, aqueous extract, plate‐shaped silver nanoparticle, zeta potential, plasmon absorption maximum, organic compounds adsorption, biogenic silver nanoparticle, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, fluconazole‐resistant Candida albicans, MTT assay, HeLa cancer cell, MCF‐7 cancer cell, Ag     

Synthesis and characterisation of neem leaf extract, 2, 3‐dehydrosalanol and quercetin dihydrate mediated silver nano particles for therapeutic applications

The utility of green silver nanoparticles (AgNPs) in veterinary medicine is steadily increasing as they have many therapeutic applications against pathogens and arthropods of livestock. In this study, green AgNPs using neem (N‐AgNPs), 2,3‐dehydrosalanol (2,3‐DHS‐AgNPs) and quercetin dihydrate (QDH‐AgNPs) were synthesised and characterised. Synthesised compounds were characterised by UV‐Vis spectroscopy and the peak absorbance was recorded at 370 nm for neem extract. For N‐AgNPs, 2,3‐DHS‐AgNPs and QDH‐AgNPs, the maximum absorbance peaks were at 430, 230 and 220 nm, respectively. The FTIR analysis confirmed the synthesis of green AgNPs. The XRD pattern of N‐AgNPs showed the peaks corresponding to whole spectra of 2 θ values ranging from 10–80. The relatively higher intensity of (111, 222) planes in face centred cubic crystalline structure supports the formation of synthesised AgNPs. In DLS analysis, the hydrodynamic diameter of neem leaf extract was found to be 259.8 nm, followed by 5.3, 6.7 and 261.8 nm for 2,3‐DHS‐AgNPs, N‐AgNPs and QDH‐AgNPs, respectively. Based on the transmission electron microscopy and scanning electron microscopy image analyses, confirmed the formation of N‐AgNPs, 2,3‐DHS‐AgNPs and QDH‐AgNPs. These eco‐friendly phyto‐AgNPs may be of use as an effective alternative to chemical control methods against the arthropods of livestock.Inspec keywords: nanoparticles, silver, nanomedicine, biomedical materials, nanofabrication, Fourier transform infrared spectra, ultraviolet spectra, visible spectra, X‐ray diffraction, light scattering, transmission electron microscopy, scanning electron microscopy, aggregation, veterinary medicineOther keywords: 2,3‐dehydrosalanol mediated silver nanoparticles, quercetin dihydrate mediated silver nanoparticles, therapeutic applications, green silver nanoparticles, veterinary medicine, Azadirachta indica, UV‐visible spectroscopy, Fourier transformed infrared analysis, X‐ray diffraction, (111) planes, (222) planes, face centred cubic crystalline structure, dynamic light scattering, hydrodynamic diameter, aqueous neem leaf extract, transmission electron microscopy, hexagonal shape, pencil head shape, cuboid shape, scanning electron microscopy, aggregation, arthropod infesting livestock, Ag, in‐vivo antiectoparasitic activity, in‐vitro antiectoparasitic activity     

Plants used in folkloric medicine of Iran are exquisite bio‐resources in production of silver nanoparticles

This study reports screening of 102 Iranian medicinal plant species belonging to 49 families for the biosynthesis of colloidal silver nanoparticles (AgNPs). Among tested plants, 37 species in 19 plant families are new bio‐resources in this regard. Water extracts of macerated plant parts are used in this survey. Six of the bioactive plants are selected for further analysis. They produced amorphous spherical 40–70 nm AgNPs. Biosynthesised colloidal AgNPs are characterised by UV–visible spectroscopy, transmission electron microscopy and scanning electron microscopy. Families of Lamiaceae (8 out of 12), Leguminosae (7 out of 9), Liliaceae (4 out of 7) contained most bio active species. No correlation noticed between bioactivity and pH of plant water‐extracts. The simple procedure used in this study may form a platform for mass production of environmentally safe and eco‐friendly green production of these multipurpose AgNPs in future; however, optimisation protocols need to be well documented.Inspec keywords: silver, nanoparticles, drugs, ultraviolet spectroscopy, visible spectroscopy, scanning electron microscopy, pH, agriculture, nanobiotechnologyOther keywords: folkloric medicine, screening, Iranian medicinal plant species, biosynthesis, colloidal silver nanoparticles production, bioresources, plant water extracts, macerated plant parts, bioactive plants, amorphous spherical AgNP, UV–visible spectroscopy, transmission electron microscopy, scanning electron microscopy, Lamiaceae, Leguminosae, Liliaceae, pH, mass production, environmentally safe green production, eco‐friendly green production     

Antibacterial potential of silver nanoparticles biosynthesised using Canarium ovatum leaves extract

Silver nanoparticles (AgNPs) have been extensively used as antibacterial agents, owing to their ease of preparation. In the present study, leaves extract of Canarium ovatum have been employed for the biosynthesis of silver nanoparticles (CO‐AgNPs). CO‐AgNPs were synthesised under very mild, eco‐friendly manner where the plant extract acted both as reducing and capping agent. These AgNPs were synthesised by taking into account several parameters, that included, time of reaction, concentration of AgNO₃, amount of extract and temperature of reaction. The optimisation studies suggested efficient synthesis of CO‐AgNPs at 25°C when 1.5 mM AgNO₃ was reduced with 1:20 ratio of plant extract for 40 min. Size determination studies done on dynamic light scattering and scanning electron microscope suggested of spherical shape nanoparticles of size 119.7 ± 7 nm and 50–80 nm, respectively. Further, characterisations were done by Fourier transform infrared and energy‐dispersive X‐ray spectroscopy to evaluate the functional groups and the purity of CO‐AgNPs. The antibacterial efficacy of CO‐AgNPs was determined against the bacterial strain Pseudomonas aeruginosa. As evident from disc diffusion method studies, CO‐AgNPs remarkably inhibited the growth of the tested microorganism. This study suggested that C. ovatum extract efficiently synthesises CO‐AgNPs with significant antibacterial properties and can be good candidates for therapeutics.Inspec keywords: antibacterial activity, nanoparticles, silver, nanofabrication, particle size, light scattering, scanning electron microscopy, Fourier transform infrared spectra, X‐ray chemical analysis, microorganisms, biomedical materials, nanomedicineOther keywords: antibacterial potential, silver nanoparticles, biosynthesis, Canarium ovatum leave extract, plant extract, reducing agent, capping agent, antibacterial agents, reaction time, reaction temperature, dynamic light scattering, scanning electron microscopy, spherical shape nanoparticles, Fourier transform infrared spectroscopy, functional groups, bacterial strain Pseudomonas aeruginosa, disc diffusion method, microorganism, energy‐dispersive X‐ray spectroscopy, temperature 25 degC, time 40 min, Ag     

Efficacy of mycosynthesised AgNPs from Earliella scabrosa as an in vitro antibacterial and wound healing agent

The silver nanoparticles (AgNPs) with their unique chemical and physical properties are proving as a new therapeutical agent. In the present study, the AgNPs synthesised from an aqueous extract of a macrofungus, Earliella scabrosa, were characterised by field emission scanning electron microscopy (FESEM), energy dispersive X‐ray analysis (EDX), high‐resolution transmission electron microscopy, X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and further evaluate for its in vitro antibacterial and wound healing efficacy. The mycosynthesised AgNPs exhibited the surface plasmon resonance peak at 410 nm with good stability over a period of a month. The FESEM and EDX analyses revealed the spherical‐shaped AgNPs of an average size of 20 nm and the presence of elemental Ag, respectively. The XRD pattern showed the crystalline nature of AgNPs. The FTIR spectra confirmed the conversion of Ag⁺ ions to AgNPs due to reduction by biomolecules of macrofungus extract. The mycosynthesised AgNPs showed effective antibacterial activity against two Gram‐positive bacteria, namely Bacillus subtilis and Staphylococcus aureus, and two Gram‐negative bacteria Escherichia coli and Pseudomonas aeruginosa. The pathogens were highly sensitive to AgNPs, whereas less sensitive to AgNO₃. The mycosynthesised AgNPs showed significant wound healing potential with 68.58% of wound closure.Inspec keywords: surface plasmon resonance, wounds, X‐ray diffraction, nanoparticles, molecular biophysics, nanomedicine, antibacterial activity, biomedical materials, reduction (chemical), silver, microorganisms, X‐ray chemical analysis, nanofabrication, transmission electron microscopy, particle size, field emission scanning electron microscopy, Fourier transform infrared spectraOther keywords: high‐resolution transmission electron microscopy, healing efficacy, mycosynthesised AgNPs, spherical‐shaped AgNPs, wound healing agent, in vitro antibacterial efficacy, Earliella scabrosa, silver nanoparticles, physical properties, chemical properties, therapeutical agent, aqueous extract, macrofungus, field emission scanning electron microscopy, FESEM, energy dispersive X‐ray analysis, EDX, X‐ray diffraction, XRD, Fourier transform infrared spectroscopy, FTIR spectroscopy, surface plasmon resonance peak, crystalline nature, biomolecules, Gram‐positive bacteria, Bacillus subtilis, Staphylococcus aureus, Gram‐negative bacteria, Escherichia coli, Pseudomonas aeruginosa, pathogens, wound closure, Ag     

Biomimetic synthesis and anticancer activity of Eurycoma longifolia branch extract‐mediated silver nanoparticles

In the present study, silver nanoparticles (AgNPs) were synthesised by adding 1 mM Ag nitrate solution to different concentrations (1%, 2.5%, 5%) of branch extracts of Eurycoma longifolia, a well known medicinal plant in South–East Asian countries. Characterisation of AgNPs was carried out using techniques such as ultraviolet–visible spectrophotometry, X‐ray diffractrometry, Fourier transform infrared–attenuated total reflection spectroscopy (FTIR–ATR), scanning electron microscopy. XRD analysis revealed face centre cubic structure of AgNPs and FTIR–ATR showed that primary and secondary amide groups in combination with the protein molecules present in the branch extract were responsible for the reduction and stabilisation of AgNPs. Furthermore, antioxidant [2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐Azino‐bis(3‐ethylbenzthiazoline‐6‐sulphonic acid)], antimicrobial and anticancer activities of AgNPs were investigated. The highest bactericidal activity of these biogenic AgNPs was found against Escherichia coli with zone inhibition of 11 mm. AgNPs exhibited significant anticancer activity against human glioma cells (DBTRG and U87) and human breast adenocarcinoma cells (MCF‐7 and MDA‐MB‐231) with IC₅₀ values of 33, 42, 60 and 38 µg/ml.Inspec keywords: biomimetics, cancer, antibacterial activity, nanoparticles, silver, microorganisms, cellular biophysics, biomedical materials, nanomedicine, nanofabrication, X‐ray diffraction, Fourier transform infrared spectra, attenuated total reflection, ultraviolet spectra, visible spectra, proteins, molecular biophysics, biochemistryOther keywords: Biomimetic synthesis, anticancer activity, Eurycoma longifolia branch extract‐mediated silver nanoparticles, nitrate solution, medicinal plant, ultraviolet‐visible spectrophotometry, X‐ray diffractometry, Fourier transform infrared‐attenuated total reflection spectroscopy, FTIR‐ATR spectroscopy, scanning electron microscopy, XRD, face centre cubic structure, primary amide groups, secondary amide groups, protein molecules, antioxidant, 2,2‐diphenyl‐1‐picrylhydrazyl, 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulphonic acid), antimicrobial activity, bactericidal activity, biogenic silver nanoparticles, Escherichia coli, zone inhibition, DBTRG human glioma cells, U87 human glioma cells, MCF‐7 human breast adenocarcinoma cells, MDA‐MB‐231 human breast adenocarcinoma cells, Ag     

Sunlight induced biosynthesis of silver nanoparticle from the bark extract of Amentotaxus assamica D.K. Ferguson and its antibacterial activity against Escherichia coli and Staphylococcus aureus

Green synthesis of nanoparticles has gained importance due to its eco‐friendly, low toxicity and cost effective nature. This study deals with the biosynthesis of silver nanoparticles (AgNPs) from the bark extract of Amentotaxus assamica. The AgNPs have been synthesised by reducing the silver ions into stable AgNPs using the bark extract of Amentotaxus assamica under the influence of sunlight irradiation. The characterisation of the biosynthesised AgNPs was carried out by UV–vis spectroscopy, X‐ray diffraction analysis (XRD), Fourier transform infrared spectroscopy, scanning electron microscopy (SEM) and energy dispersive X‐ray analysis. The UV–vis spectrum showed a broad peak at 472 nm. Also, the XRD confirmed the crystalline structure of the AgNPs. Moreover, the SEM analysis revealed that the biosynthesised AgNPs were spherical in shape. Also, dynamic light scattering techniques were used to evaluate the size distribution profile of the biosynthesised AgNPs. Furthermore, the biosynthesised AgNPs showed a prominent inhibitory effect against both Escherichia coli (MTCC 111) and Staphylococcus aureus (MTCC 97). Thus the biosynthesis of AgNPs from the bark extract of Amentotaxus assamica is found to eco‐friendly way of producing AgNPs compared to chemical method.Inspec keywords: X‐ray chemical analysis, microorganisms, transmission electron microscopy, nanoparticles, toxicology, scanning electron microscopy, ultraviolet spectra, particle size, Fourier transform spectra, X‐ray diffraction, antibacterial activity, visible spectra, infrared spectra, nanomedicine, silverOther keywords: stable AgNP, biosynthesised AgNP, SEM analysis, sunlight irradiation, silver ions, silver nanoparticle, amentotaxus assamica, biosynthesis, escherichia coli     

Green synthesis,characterisation and biological evaluation of AgNPs using Agave americana,Mentha spicata and Mangifera indica aqueous leaves extract

The current study was performed to synthesize stable, eco‐friendly and bio‐compatible silver nano‐particles (AgNPs) of Agave americana, Mentha spicata and Mangifera indica leaves and to screen them for biological activities. The ultraviolet‐visible spectroscopic analysis revealed that λ‐max for AgNPs range from 350–500 nm. All AgNPs possessed polycrystalline structure as notified as intense graphical peaks in complete spectrum of 20 values ranging from 10–80° in X‐ray diffraction measurements and supported by scanning electron microscopy data. The size of the nano‐particles was confirmed by transmission electron microscopy (30–150 nm). Mass loss at variable temperatures was evaluated by simultaneous thermogravimetric and differential thermal analysis revealed reduction in mass and activity of compounds was notified by temperature increase from 200 to 800 °C, thus concluding it as thermally sensitive compounds. A. americana AgNPs showed significant (96%) activity against Methicillin resistant Staphylococcus aureus, Escherichia coli (95%) and Fusarium oxysporum (89%). Good antioxidant activity was shown by M. spicata AgNPs at 300 µl (79%). M. indica AgNPs showed significant phytotoxic activity (88%) at highest concentration. No haemagglutination reaction was observed for the test samples. The above results revealed that AgNPs synthesized from selected plant species possesses significant antimicrobial and phytotoxic effect.Inspec keywords: silver, nanoparticles, nanofabrication, X‐ray diffraction, transmission electron microscopy, scanning electron microscopy, differential thermal analysis, microorganisms, antibacterial activity, nanomedicine, particle size, toxicologyOther keywords: green synthesis, biological evaluation, Agave americana aqueous leave extract, Mentha spicata aqueous leave extract, Mangifera indica aqueous leave extract, stable ecofriendly biocompatible silver nanoparticles, ultraviolet‐visible spectroscopy, polycrystalline structure, X‐ray diffraction, scanning electron microscopy, nanoparticle size, transmission electron microscopy, thermogravimetric analysis, differential thermal analysis, mass loss, thermally sensitive compounds, Methicillin resistant Staphylococcus aureus, Escherichia coli, Fusarium oxysporum, antioxidant activity, phytotoxic activity, plant species, antimicrobial effect, temperature 200 degC to 800 degC, Ag     

Facile phyto‐mediated synthesis of silver nanoparticles using Chinese winter jujube (Ziziphus jujuba Mill. cv. Dongzao) extract and their antibacterial/catalytic properties

The aqueous extract of Chinese winter jujube (Ziziphus jujuba Mill. cv. Dongzao) was used as reducing and capping agents for the synthesis of silver nanoparticles (AgNPs) for the first time. The resulting AgNPs were characterised by UV/Visible (UV–Vis) spectroscopy, atomic force microscope, transmission electron microscopy, selected area electron diffraction, X‐ray diffraction, scanning electron microscopy, energy‐dispersive X‐ray and Fourier transform infrared spectroscopy (FTIR). The colloidal solution of AgNPs gave a maximum UV–Vis absorbance at 446 nm. The synthesised nanoparticles were almost in the spherical shapes with an average size of 11.5 ± 4. 8 nm. FTIR spectra were applied to identify the functional groups which were possibly responsible for the conversion of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with the biomolecules in the extract. The biosynthesised AgNPs showed a remarkable catalytic activity at room temperature, and they also showed good antibacterial properties against Escherichia coli and Staphylococcus aureus.Inspec keywords: silver, nanoparticles, nanofabrication, antibacterial activity, biomedical materials, nanobiotechnology, scanning electron microscopy, X‐ray diffraction, transmission electron microscopy, ultraviolet spectra, visible spectra, X‐ray chemical analysis, Fourier transform infrared spectra, catalysisOther keywords: wavelength 446 nm, temperature 293 K to 298 K, Ag, Escherichia coli, Staphylococcus aureus, biomolecules, catalytic activity, metal ions, colloidal solution, FTIR spectra, UV‐vis absorbance, TEM, SEM, XRD, Fourier transform infrared spectroscopy, energy‐dispersive X‐ray spectroscopy, scanning electron microscopy, X‐ray diffraction, selected area electron diffraction, transmission electron microscopy, atomic force microscopy, UV‐visible spectroscopy, catalytic properties, antibacterial properties, Chinese winter jujube extract, silver nanoparticles, facile phyto‐mediated synthesis     

Evaluation of antimicrobial activity of synthesised silver nanoparticles using Thymus kotschyanus aqueous extract

Development of a green chemistry process for the synthesis of silver nanoparticles (AgNPs) has become a focus of interest. Characteristics of AgNPs were determined using techniques, such as ultraviolet–visible spectroscopy (UV–vis), Fourier transform infrared (FTIR) analysis, scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy and X‐ray diffraction (XRD). The synthesised AgNPs using Thymus kotschyanus had the most growth inhibition against gram‐positive bacteria such as Staphylococcus aureus and Bacillus subtilise, while the growth inhibition of AgNPs at 1000–500 µg/ml occurred against Klebsiella pneumonia and at 1000–250 µg/ml of AgNPs was observed against E. coli. The UV–vis absorption spectra confirmed the formation of the AgNPs with the characteristic peak at 415 nm and SEM micrograph acknowledged spherical particles in a nanosize range. FTIR measured the possible biomolecules that are responsible for stabilisation of AgNPs. XRD analysis exhibited the crystalline nature of AgNPs and showed face‐centred cubic structure. The synthesised AgNPs revealed significant antibacterial activity against gram‐positive bacteria.Inspec keywords: visible spectra, microorganisms, ultraviolet spectra, biomedical materials, nanofabrication, nanoparticles, X‐ray diffraction, scanning electron microscopy, molecular biophysics, X‐ray chemical analysis, nanomedicine, silver, antibacterial activity, Fourier transform infrared spectraOther keywords: green chemistry process, ultraviolet–visible spectroscopy, gram‐positive bacteria, silver nanoparticles, Thymus kotschyanus aqueous extract, UV–vis spectroscopy, Fourier transform infrared spectroscopy, FTIR analysis, scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, SEM micrograph, X‐ray diffraction, XRD, Staphylococcus aureus, Bacillus subtilise, Klebsiella pneumonia, E. coli, UV–vis absorption spectra, face‐centred cubic structure, antibacterial activity, antimicrobial activity, wavelength 415.0 nm, Ag     

Antioxidant,antibacterial and anticancer properties of phyto ‐ synthesised Artemisia quttensis Podlech extract mediated AgNPs

The focus of this study is on a rapid and cost‐effective approach for the synthesis of silver nanoparticles (AgNPs) using Artemisia quttensis Podlech aerial parts extract and assessment of their antioxidant, antibacterial and anticancer activities. The prepared AgNPs were determined by ultraviolet–visible spectroscopy, X‐ray diffraction, Fourier transform infra‐red spectroscopy, transmission electron microscopy, scanning electron microscopy, energy‐dispersive spectroscopy, and dynamic light scattering and zeta‐potential analysis. The AgNPs and A. quttensis extract were evaluated for their antiradical scavenging activity by 2, 2‐diphenyl, 1‐picryl hydrazyl assay and anticancer activity against colon cancer (human colorectal adenocarcinoma cell line 29) compared with normal human embryonic kidney (HEK293) cells. Also, the prepared AgNPs were studied for its antibacterial activity. The AgNPs revealed a higher antioxidant activity compared with A. quttensis extract alone. The phyto‐synthesised AgNPs and A. quttensis extract showed a dose–response cytotoxicity effect against HT29 and HEK293 cells. As evidenced by Annexin V/propidium iodide staining, the number of apoptotic HT29 cells was significantly enhanced, following treatment with AgNPs as compared with untreated cells. Besides, the antibacterial property of the AgNPs indicated a significant effect against the selected pathogenic bacteria. These present obtained results show the potential applications of phyto‐synthesised AgNPs using A. quttensis aerial parts extract.Inspec keywords: nanoparticles, silver, nanomedicine, cancer, transmission electron microscopy, ultraviolet spectroscopy, visible spectroscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, electrokinetic effects, kidney, cellular biophysics, antibacterial activity, toxicology, patient treatmentOther keywords: anticancer properties, antibacterial properties, antioxidant properties, phytosynthesised Artemisia quttensis Podlech extract mediated AgNP, ultraviolet‐visible spectroscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, energy‐dispersive spectroscopy, dynamic light scattering, zeta‐potential analysis, antiradical scavenging activity, 2,2‐diphenyl, 1‐picryl hydrazyl assay, anticancer activity, HT29 colon cancer, human embryonic kidney cells, HEK293 cells, A. quttensis extract, dose‐response cytotoxicity effect, Annexin V staining, apoptotic HT29 cells, pathogenic bacteria, propidium iodide staining, Ag