Antibacterial activity of carbon nanoparticles isolated from chimney soot

Carbon nanoparticles (CNPs) are isolated from chimney soot and characterised by various tools such as X‐ray diffraction, scanning electron microscopy, transmission electron microscopy and ultraviolet–visible spectroscopy. The X‐ray diffraction studies confirm the presence of C₆₀ nanoparticles in the isolated sample. The thermal properties of the prepared CNPs are recorded using thermogravimetric analysis and differential thermal analysis. The analysis of the antibacterial activity of the synthesised CNPs against selected Gram‐positive and Gram‐negative bacterial strains is also investigated. The systematic study confirms that CNPs collected from chimney soot exhibit good antibacterial potency against Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumoniae, and Proteus mirabilis.Inspec keywords: ultraviolet spectra, scanning electron microscopy, visible spectra, differential thermal analysis, thermal analysis, antibacterial activity, nanoparticles, X‐ray diffraction, nanofabrication, transmission electron microscopy, carbonOther keywords: chimney soot, transmission electron microscopy, ultraviolet–visible spectroscopy, thermal properties, thermogravimetric analysis, differential thermal analysis, antibacterial activity, carbon nanoparticles, X‐ray diffraction study, gram‐positive bacterial strains, gram‐negative bacterial strains, antibacterial potency, scanning electron microscopy, C₆₀      

Structural and physicochemical properties of Rheum emodi mediated Mg(OH)2 nanoparticles and their antibacterial and cytotoxic potential

In the present investigation, Rheum emodi roots extract mediated magnesium hydroxide nanoparticles [Mg(OH)₂ NPs] through the bio‐inspired experimental technique were synthesised. Mg(OH)₂ NPs were characterised by using various characterisation techniques such as field emission scanning electron microscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and ultraviolet–visible spectroscopy. The formation of Mg(OH)₂ NPs was confirmed by X‐ray diffraction. The structural analysis confirmed the hexagonal crystal symmetry of Mg(OH)₂ NPs with space group P‐3m1 and space group no. 164 using the Rietveld refinement technique. TEM micrographs illustrated the nano‐size formation of Mg(OH)₂ NPs of spherical shape and size ∼14.86 nm. With the aid of FTIR data, plant metabolites such as anthraquinones have been identified as a stabilising and reducing agent for the synthesis of biogenic Mg(OH)₂ NPs. The synthesised Mg(OH)₂ NPs showed antimicrobial and cytotoxic potential against Gram‐negative and Gram‐positive bacteria such as Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) and MDA‐MB‐231 human breast cancer cell lines.Inspec keywords: antibacterial activity, microorganisms, visible spectra, cancer, X‐ray diffraction, cellular biophysics, nanomedicine, ultraviolet spectra, nanoparticles, transmission electron microscopy, nanofabrication, field emission scanning electron microscopy, Fourier transform infrared spectra, particle size, magnesium compounds, space groups, toxicologyOther keywords: physicochemical properties, structural properties, Rheum emodi root extract mediated magnesium hydroxide nanoparticles, bio‐inspired experimental technique, field emission scanning electron microscopy, transmission electron microscopy, TEM, Fourier transform infrared spectroscopy, FTIR spectroscopy, ultraviolet‐visible spectroscopy, X‐ray diffraction, hexagonal crystal symmetry, space group P‐3m1, space group no. 164, Rietveld refinement technique, nanosize formation, plant metabolites, spherical shape, antibacterial potential, cytotoxic potential, reducing agent, anthraquinones, stabilising agent, Gram‐positive bacteria, Gram‐negative bacteria, Escherichia coli, Staphylococcus aureus, MDA‐MB‐231 human breast cancer cell lines, Mg(OH)₂      

Green synthesis of copper oxide nanoparticles using aqueous extract of Convolvulus percicus L. as reusable catalysts in cross‐ coupling reactions and their antibacterial activity

CuO nanoparticles (NPs) were prepared by Convolvulus percicus leaves extract as a reducing and stabilising agent. The green synthesised copper oxide NPs was characterised by transmission electron microscope, energy dispersive X‐Ray spectroscopy, X‐ray diffraction, Fourier transform infrared and ultraviolet‐visible analysis. The activities of the CuO NPs as catalyst were tested in the formation of C‐N and C‐O bonds. The N ‐arylated and O ‐arylated products of amides, N‐H heterocycles and phenols were obtained in excellent yields. Furthermore, the separation and recovery of copper oxide NPs was very simple, effective and economical. The recovered catalyst can be reused several times without significant loss of its catalytic activity. Moreover, the antibacterial activity of these NPs was tested against two human pathogenic microbes and showed significant antimicrobial activity against these pathogenic bacteria.Inspec keywords: copper compounds, nanoparticles, nanomedicine, antibacterial activity, biomedical materials, nanofabrication, microorganisms, catalysts, transmission electron microscopy, X‐ray chemical analysis, X‐ray diffraction, Fourier transform spectra, infrared spectra, ultraviolet spectra, visible spectra, catalysisOther keywords: green synthesis, copper oxide nanoparticles, Convolvulus percicus L. aqueous extract, reusable catalysts, cross‐coupling reactions, antibacterial activity, reducing agent, stabilising agent, transmission electron microscope, energy dispersive X‐ray spectroscopy, X‐ray diffraction, Fourier transform infrared spectra, ultraviolet‐visible spectra, C‐N bonds, C‐O bonds, N‐arylated products, O‐arylated products, amides, N‐H heterocycles, phenols, catalytic activity, human pathogenic microbes, antimicrobial activity, CuO     

Role of Ribes khorassanicum in the biosynthesis of AgNPs and their antibacterial properties

Silver nanoparticles (AgNPs) have been biosynthesised through the extracts of Ribes khorassanicum fruits, which served as the reducing agents and capping agents. Biosynthesised AgNPs have been found to be ultraviolet–visible (UV–vis) absorption spectra since they have displayed one surface plasmon resonance peak at 438 nm, attesting the formation of spherical NPs. These particles have been characterised by UV–vis, field‐emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy analysis. The formation of AgNPs at 1.0 mM concentration of AgNO₃ has resulted in NPs that contained mean diameters in a range of 20–40 nm. The green‐synthesised AgNPs have demonstrated high antibacterial effect against pathogenic bacteria (i.e. Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa). Biosynthesising metal NPs through plant extracts can serve as the facile and eco‐friendly alternative for chemical and/or physical methods that are utilised for large‐scale nanometal fabrication in various medical and industrial applications.Inspec keywords: X‐ray diffraction, X‐ray chemical analysis, nanofabrication, surface plasmon resonance, nanoparticles, antibacterial activity, microorganisms, scanning electron microscopy, silver, nanomedicine, visible spectra, ultraviolet spectra, transmission electron microscopy, Fourier transform infrared spectra, field emission scanning electron microscopy, biomedical materialsOther keywords: antibacterial properties, silver nanoparticles, reducing agents, capping agents, surface plasmon resonance peak, spherical NPs, field‐emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, transmission electron microscopy analysis, plant extracts, ultraviolet‐visible absorption spectra, Fourier transform infrared spectroscopy, antibacterial effect, Ribes khorassanicum fruits, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, surface plasmon resonance, AgNO₃ , Ag     

Biosynthesis of the CuO nanoparticles using Euphorbia Chamaesyce leaf extract and investigation of their catalytic activity for the reduction of 4‐nitrophenol

Through this study an eco‐friendly, simple, efficient, cheap and biocompatible approach to the biosynthesis and stabilisation of CuO nanoparticles (NPs) using the Euphorbia Chamaesyce leaf extract is presented. The CuO NPs were monitored and characterised by field emission scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier transformed infrared spectroscopy, transmission electron microscope and UV‐visible spectroscopy. The biosynthesised CuO NPs showed good catalytic activity for the reduction of 4‐nitrophenol (4‐NP) in water during 180 s and reused 4 times without considerable loss of activity.Inspec keywords: copper compounds, nanoparticles, nanofabrication, catalysis, reduction (chemical), field emission electron microscopy, X‐ray chemical analysis, Fourier transform infrared spectra, transmission electron microscopy, ultraviolet spectra, visible spectraOther keywords: biosynthesis, CuO nanoparticles, Euphorbia Chamaesyce leaf extract, catalytic activity, 4‐nitrophenol reduction, nanoparticle stabilisation, field emission scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier transformed infrared spectroscopy, transmission electron microscope, UV‐visible spectroscopy, CuO     

Catalytic reduction of 2,4‐dinitrophenylhydrazine by cuttlebone supported Pd NPs prepared using Conium maculatum leaf extract

A facile and green process to synthesise cuttlebone supported palladium nanoparticles (Pd NPs/cuttlebone) is reported using Conium maculatum leaf extract and in the absence of chemical solvents and hazardous materials. The antioxidant content of the C. maculatum leaf extract played a significant role in converting Pd²⁺ ions to Pd NPs. Various techniques were used for the characterisation of the Pd NPs/cuttlebone such as field‐emission scanning electron microscopy, X‐ray diffraction, energy dispersive X‐ray spectroscopy, Fourier transform infrared and ultraviolet–visible spectroscopy. This Pd NPs/cuttlebone showed excellent catalytic activity in the reduction of 2,4‐dinitrophenylhydrazine to 2,4‐diaminophenylhydrazine by sodium borohydride as the source of hydrogen at ambient condition. The catalyst could be separated and recycled up to five cycles with no loss of its activity.Inspec keywords: catalysis, catalysts, chemical engineering, palladium, nanoparticles, field emission electron microscopy, scanning electron microscopy, X‐ray diffraction, X‐ray chemical analysis, sodium compounds, ultraviolet spectroscopy, visible spectroscopyOther keywords: catalytic reduction, 2,4‐dinitrophenylhydrazine, cuttlebone, Conium maculatum leaf extract, green process, palladium nanoparticles, antioxidant content, field‐emission scanning electron microscopy, X‐ray diffraction, energy dispersive X‐ray spectroscopy, Fourier transform infrared, ultraviolet–visible spectroscopy, 2,4‐diaminophenylhydrazine, sodium borohydride     

Green synthesis of CuO nanoparticles loaded on the seashell surface using Rumex crispus seeds extract and its catalytic applications for reduction of dyes

In this study, CuO nanoparticles supported on the seashell (CuO NPs/seashell) was prepared using Rumex crispus seeds extract as a chelating and capping agent. The prepared nanocomposite was characterised by Fourier transform infrared spectroscopy, X‐ray diffraction, field emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy and transmission electron microscopy. The particle size of CuO NPs on the seashell sheets was in the range of 8–60 nm. Catalytic ability of CuO NPs/seashell was investigated for the reduction of 4‐nitrophenol (4‐NP) and Congo red (CR). It was observed that catalyst can be easily recovered and reused several times without any significant loss of catalytic efficiency.Inspec keywords: nanocomposites, nanoparticles, catalysis, dyes, Fourier transform infrared spectra, X‐ray diffraction, field emission electron microscopy, scanning electron microscopy, X‐ray chemical analysis, transmission electron microscopy, particle size, copper compoundsOther keywords: CuO, size 8 nm to 60 nm, Congo red, 4‐nitrophenol, particle size, transmission electron microscopy, energy dispersive X‐ray spectroscopy, field emission scanning electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, nanocomposite, capping agent, chelating agent, dye reduction, catalytic application, Rumex crispus seeds extract, seashell surface, nanoparticles, green synthesis     

Green synthesis of copper oxide nanoparticles and mosquito larvicidal activity against dengue,zika and chikungunya causing vector Aedes aegypti

In the present study, high purity copper oxide nanoparticles (NPs) were synthesised using Tridax procumbens leaf extract. Green syntheses of nano‐mosquitocides rely on plant compounds as reducing and stabilising agents. Copper oxide NPs were characterised using X‐ray diffraction (XRD) analysis, Fourier transform infrared (FT‐IR), Field‐emission scanning electron microscopy with energy dispersive spectroscopy, Ultraviolet–visible spectrophotometry and fluorescence spectroscopy. XRD studies of the NPs indicate crystalline nature which was perfectly matching with a monoclinic structure of bulk CuO with an average crystallite size of 16 nm. Formation of copper oxide NPs was confirmed by FT‐IR studies and photoluminescence spectra with emission peaks at 331, 411 and 433 nm were assigned to a near‐band‐edge emission band of CuO in the UV, violet and blue region. Gas chromatography–mass spectrometry studies inferred the phytochemical constituents of the leaf extract. Larvicidal activity of synthesised NPs using T. procumbens leaf extract was tested against Aedes aegypti species (dengue, chikungunya, zika and yellow fever transmit vector).Inspec keywords: photoluminescence, spectrophotometry, thermal analysis, chromatography, nanoparticles, antibacterial activity, field emission electron microscopy, microorganisms, wide band gap semiconductors, scanning electron microscopy, X‐ray diffraction, copper compounds, ultraviolet spectra, nanofabrication, X‐ray chemical analysis, crystallites, visible spectra, field emission scanning electron microscopy, nanobiotechnology, semiconductor materials, semiconductor growth, fluorescence, mass spectraOther keywords: energy dispersive spectroscopy, ultraviolet–visual spectrophotometry, fluorescence spectroscopy, chikungunya, green synthesis, mosquito larvicidal activity, zika, X‐ray diffraction analysis, field‐emission scanning electron microscopy, XRD, gas chromatography–mass spectrometry, copper oxide nanoparticles, dengue, tridax procumben leaf extract, nanomosquitocides, FTIR, monoclinic structure, crystallite size, photoluminescence spectra, near‐band‐edge emission band, phytochemical constituents, Aedes aegypti species, yellow fever transmit vector, CuO     

Microbicidal activity of TiO2 nanoparticles synthesised by sol–gel method

In this study, the authors investigated antimicrobial activity of TiO₂ nanoparticles (NPs) synthesised by sol–gel method. As synthesised TiO₂ NPs were characterised by X‐ray diffraction, scanning electron microscopy and ultraviolet‐visible absorption spectroscopy. The antimicrobial activity of calcined TiO₂ nanoparticle samples was examined in day light on Gram positive bacteria (Staphylococcus aureus, Streptococcus pneumonia and Bacillus subtilis), Gram negative bacteria (Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungal test pathogen Candida albicans. The synthesised TiO₂ NPs were found to be effective in visible light against Streptococcus pneumonia, Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa and Candida albicans.Inspec keywords: titanium compounds, microorganisms, nanomedicine, biomedical materials, nanofabrication, sol‐gel processing, ultraviolet spectra, visible spectra, X‐ray diffraction, scanning electron microscopy, nanoparticles, antibacterial activityOther keywords: microbicidal activity, titanium dioxide nanoparticle, sol‐gel method, antimicrobial activity, X‐ray diffraction, scanning electron microscopy, ultraviolet‐visible absorption spectroscopy, Gram positive bacteria, Staphylococcus aureus, Streptococcus pneumonia, Bacillus subtilis, TiO₂ , Candida albicans, fungal test pathogen, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Gram negative bacteria     

Sustainable green synthesis of silver nanoparticles by using Rangoon creeper leaves extract and their spectral analysis and anti‐bacterial studies

The plant‐based biological molecules possess exceptionally controlled assembling properties to make them suitable in the synthesis of metal nanoparticles. In the present study, an efficient simple one‐pot method was employed for the synthesis of silver nanoparticles (SNPs) from the Rangoon creeper (RC) aqueous leaf extract. Biomolecules present in the leaf extract play a significant role as reducing agent as well as capping agent in the formation of RC‐SNPs. The formation of RC‐SNPs was confirmed by using several analytical techniques such as Fourier‐transform infrared spectroscopy and ultraviolet–visible spectrophotometer studies. The presence of a sharp surface plasmon resonance peak at 449 nm showed the formation of RC‐SNPs. X‐ray diffraction analysis showed the crystalline nature of the RC‐SNPs with a face‐centred cubic structure. Elemental analysis of RC‐SNPs was done by using energy‐dispersive X‐ray spectroscopy and X‐ray photoelectron spectroscopy. The morphology of RC‐SNPs was examined by transmission electron microscopy (TEM) in the nano range 12 nm, and thermogravimetric‐differential thermal analysis demonstrated the mechanical strength of RC‐SNPs at various temperatures. The authors’ newly synthesised RC‐SNPs exhibited significant anti‐bacterial activity against Staphylococcus aureus and Escherichia coli. Inspec keywords: silver, nanoparticles, X‐ray photoelectron spectra, antibacterial activity, ultraviolet spectra, microorganisms, X‐ray chemical analysis, differential thermal analysis, X‐ray diffraction, transmission electron microscopy, visible spectra, nanofabrication, surface plasmon resonance, Fourier transform infrared spectra, mechanical strengthOther keywords: silver nanoparticles, ultraviolet–visible spectrophotometry, antibacterial activity, sustainable green synthesis, plant‐based biological molecules, assembling properties, reducing agent, capping agent, Fourier‐transform infrared spectroscopy, surface plasmon resonance, Rangoon creeper aqueous leaf extract, X‐ray diffraction, face‐centred cubic structure, elemental analysis, energy‐dispersive X‐ray spectroscopy, X‐ray photoelectron spectroscopy, transmission electron microscopy, TEM, thermogravimetric‐differential thermal analysis, mechanical strength, Staphylococcus aureus, Escherichia coli, Ag     

Biosynthesis of Selenium Nanoparticles using yeast Nematospora coryli and examination of their anti‐candida and anti‐oxidant activities

Selenium (Se) is a rare and essential element for the human body and other living organisms because of its role in the structure of several proteins and having anti‐oxidant properties to reduce oxidative stress at cells. Some microorganisms can absorb Se oxyanions and convert them into zero‐valent Se (Se0) in the nanoscale dimensions, which can be used for producing Se nanoparticles (SeNPs). In the present study, SeNPs were intracellularly biosynthesised by yeast Nematospora coryli, which is an inexpensive method and does not involve using materials hazardous for human and environment. The produced NPs were refined by a two‐phase system and then characterised and identified by ultraviolet–visible, X‐ray diffraction, X‐ray fluorescence, transmission electron microscope, and Fourier transform infrared spectroscopy analyses. The structural analysis of biosynthesised SeNPs showed spherical‐shaped NPs with size ranging from 50 to 250 nm. Also, extracted NPs were applied to explore their anti‐candida and anti‐oxidant activities. The results of this investigation confirm the biological properties of Se.Inspec keywords: X‐ray diffraction, microorganisms, oxidation, transmission electron microscopy, reduction (chemical), nanomedicine, biomedical materials, visible spectra, nanoparticles, proteins, nanofabrication, selenium, ultraviolet spectra, particle size, Fourier transform infrared spectra, antibacterial activityOther keywords: proteins, oxidative stress, Se oxyanions, yeast, biosynthesised SeNPs, anti‐oxidant activities, human body, living organisms, Se nanoparticles, Nematospora coryli, anti‐candida activities, biosynthesis, ultraviolet–visible analysis, X‐ray diffraction, X‐ray fluorescence, transmission electron microscope, Fourier transform infrared spectroscopy, structural analysis, size 50.0 nm to 250.0 nm, Se     

Solvothermal‐assisted green synthesis of hybrid Chi‐Fe3 O4 nanocomposites: a potential antibacterial and antibiofilm material

In this present study, a hybrid Chi‐Fe₃ O₄ was prepared, characterised and evaluated for its antibacterial and antibiofilm potential against Staphylococcus aureus and Staphylococcus marcescens bacterial pathogens. Intense peak around 260 nm in the ultraviolet–visible spectrum specify the formation of magnetite nanoparticles. Spherical‐shaped particles with less agglomeration and particle size distribution of 3.78–46.40 nm were observed using transmission electron microscopy analysis and strong interaction of chitosan with the surface of magnetite nanoparticles was studied using field emission scanning microscopy (FESEM). X‐ray diffraction analysis exhibited the polycrystalline and spinel structure configuration of the nanocomposite. Presence of Fe and O, C and Cl elements were confirmed using energy dispersive X‐ray microanalysis. Fourier transform infrared spectroscopic analysis showed the reduction and formation of Chi‐Fe₃ O₄ nanocomposite. The antibacterial activity by deformation of the bacterial cell walls on treatment with Chi‐Fe₃ O₄ nanocomposite and its interaction was visualised using FESEM and the antibiofilm activity was determined using antibiofilm assay. In conclusion, this present study shows the green synthesis of Chi‐Fe₃ O₄ nanocomposite and evaluation of its antibacterial and antibiofilm potential, proving its significance in medical and biological applicationsInspec keywords: visible spectra, particle size, magnetic particles, nanocomposites, nanoparticles, X‐ray diffraction, nanofabrication, transmission electron microscopy, X‐ray chemical analysis, nanomagnetics, microorganisms, antibacterial activity, iron compounds, ultraviolet spectra, biomedical materials, field emission scanning electron microscopy, Fourier transform infrared spectra, filled polymers, crystal growth from solution, polymer structureOther keywords: potential antibacterial material, antibiofilm potential, magnetite nanoparticles, solvothermal‐assisted green synthesis, hybrid Chi‐Fe₃ O₄ nanocomposites, staphylococcus aureus, staphylococcus marcescens, bacterial pathogens, ultraviolet–visible spectrum, spherical‐shaped particles, particle size, transmission electron microscopy, FESEM, field emission scanning electron microscopy, X‐ray diffraction, spinel structure, polycrystalline structure, energy dispersive X‐ray microanalysis, Fourier transform infrared spectroscopic analysis, deformation, bacterial cell walls, Fe₃ O₄      

Efficacy of mycosynthesised AgNPs from Earliella scabrosa as an in vitro antibacterial and wound healing agent

The silver nanoparticles (AgNPs) with their unique chemical and physical properties are proving as a new therapeutical agent. In the present study, the AgNPs synthesised from an aqueous extract of a macrofungus, Earliella scabrosa, were characterised by field emission scanning electron microscopy (FESEM), energy dispersive X‐ray analysis (EDX), high‐resolution transmission electron microscopy, X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and further evaluate for its in vitro antibacterial and wound healing efficacy. The mycosynthesised AgNPs exhibited the surface plasmon resonance peak at 410 nm with good stability over a period of a month. The FESEM and EDX analyses revealed the spherical‐shaped AgNPs of an average size of 20 nm and the presence of elemental Ag, respectively. The XRD pattern showed the crystalline nature of AgNPs. The FTIR spectra confirmed the conversion of Ag⁺ ions to AgNPs due to reduction by biomolecules of macrofungus extract. The mycosynthesised AgNPs showed effective antibacterial activity against two Gram‐positive bacteria, namely Bacillus subtilis and Staphylococcus aureus, and two Gram‐negative bacteria Escherichia coli and Pseudomonas aeruginosa. The pathogens were highly sensitive to AgNPs, whereas less sensitive to AgNO₃. The mycosynthesised AgNPs showed significant wound healing potential with 68.58% of wound closure.Inspec keywords: surface plasmon resonance, wounds, X‐ray diffraction, nanoparticles, molecular biophysics, nanomedicine, antibacterial activity, biomedical materials, reduction (chemical), silver, microorganisms, X‐ray chemical analysis, nanofabrication, transmission electron microscopy, particle size, field emission scanning electron microscopy, Fourier transform infrared spectraOther keywords: high‐resolution transmission electron microscopy, healing efficacy, mycosynthesised AgNPs, spherical‐shaped AgNPs, wound healing agent, in vitro antibacterial efficacy, Earliella scabrosa, silver nanoparticles, physical properties, chemical properties, therapeutical agent, aqueous extract, macrofungus, field emission scanning electron microscopy, FESEM, energy dispersive X‐ray analysis, EDX, X‐ray diffraction, XRD, Fourier transform infrared spectroscopy, FTIR spectroscopy, surface plasmon resonance peak, crystalline nature, biomolecules, Gram‐positive bacteria, Bacillus subtilis, Staphylococcus aureus, Gram‐negative bacteria, Escherichia coli, Pseudomonas aeruginosa, pathogens, wound closure, Ag     

Evaluating the effect of green synthesised copper oxide nanoparticles on oxidative stress and mitochondrial function using murine model

Green synthesis of metal nanoparticles (NPs) has now received the attention of researchers due to ease of preparation and its potential to overcome hazards of these chemicals for an eco‐friendly milieu. In this study, copper oxide (CuO) NPs were synthesised via Desmodium gangeticum aqueous root extract and standard chemical method, further characterised by UV–visible spectroscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, Thermogravimetric analysis and scanning electron microscopy. The nephrotoxicity of the NP obtained from two routes were compared and evaluated at subcellular level in Wistar rat, renal proximal epithelial cells (LLC PK1 cell lines) and isolated renal mitochondria. CuO NP synthesised by chemical route showed prominent nephrotoxicity measured via adverse cytotoxicity to LLC PK1 cells, elevated renal oxidative stress and damage to renal tissue (determined by impaired alanine transaminase, aspartate transaminase, urea, uric acid and creatinine in the blood). However, at the level of cell organelle, CuO NP from both routes are non‐toxic to mitochondrial functional activity. The authors’ finding suggests that CuO NP synthesised by chemical route may induce nephrotoxicity, but may be overcome by co‐administration of antioxidants, as it is not mito‐toxic.Inspec keywords: cellular biophysics, scanning electron microscopy, toxicology, nanomedicine, oxidation, nanoparticles, enzymes, blood, visible spectra, X‐ray diffraction, biochemistry, nanofabrication, antibacterial activity, ultraviolet spectra, copper compounds, Fourier transform infrared spectra, molecular biophysics, thermal analysis, biological tissuesOther keywords: green synthesised copper oxide nanoparticles, murine model, metal nanoparticles, chemicals, eco‐friendly milieu, copper oxide NPs, standard chemical method, X‐ray diffraction, scanning electron microscopy, subcellular level, renal proximal epithelial cells, LLC PK1 cell lines, renal mitochondria, renal tissue, cell organelle, mitochondrial functional activity, UV‐visible spectroscopy, Fourier transform infrared spectroscopy, nephrotoxicity, renal oxidative stress, Desmodium gangeticum aqueous root extract, thermogravimetric analysis, Wistar rat, cytotoxicity, impaired alanine transaminase, aspartate transaminase, urea, uric acid, creatinine, blood, CuO     

Simple,green approach for the synthesis of solid support‐embedded PdNPs for ligand exchange

Green approaches have the potential to significantly reduce the costs and environmental impact of chemical syntheses. Here, the authors used green tea (GT) leaf extract to synthesise and anchor palladium nanoparticles (PdNPs) to silica. The synthesised PdNPs in GT extract were characterised by ultraviolet–visible spectroscopy, Fourier‐transform infrared spectroscopy, X‐ray diffraction, and transmission electron microscopy. PdNPs primarily formed as capped NPs dispersed in GT extract before reduction completed after 24 h. This capped phytochemical solution was employed as a green precursor solution to synthesise PdNP‐embedded solid supports. The morphology of PdNPs anchored to silica differed to that of PdNPs in solution. Silica‐embedded PdNPs was employed as a new ligand exchanger to isolate trace polycyclic aromatic sulphur heterocycles from a hydrocarbon matrix. The isolation efficiency of the new, greener ligand exchanger was the same as an efficient chemical ligand exchanger and may, therefore, hold promise for future applications.Inspec keywords: nanofabrication, palladium, visible spectra, transmission electron microscopy, nanoparticles, reduction (chemical), ultraviolet spectra, X‐ray diffraction, Fourier transform infrared spectra, surface morphologyOther keywords: ultraviolet–visible spectroscopy, Fourier‐transform infrared spectroscopy, transmission electron microscopy, phytochemical solution, green precursor solution, PdNP‐embedded solid supports, solid support‐embedded PdNPs, green tea leaf extract, chemical ligand exchanger, anchor palladium nanoparticles, X‐ray diffraction, isolate trace polycyclic aromatic sulphur heterocycles, hydrocarbon matrix, green synthesis, time 24.0 hour, Pd     

Biosynthesis of zinc oxide nanoparticles by petals extract of Rosa indica L., its formulation as nail paint and evaluation of antifungal activity against fungi causing onychomycosis

Aim: The authors report the biological synthesis of zinc oxide nanoparticles (ZnO‐NPs) from the petals extract of Rosa indica L. (rose). Its efficacy was evaluated against two dermatophytes: namely: Trichophyton mentagrophytes and Microsporum canis which cause onychomycosis. The activity of antibiotics against the tested dermatophytes was enhanced, when evaluated in combination with ZnO‐NPs. Methods and results: The synthesised ZnO‐NPs were preliminary detected by using ultraviolet UV visible spectroscopy, which showed specific absorbance. The ZnO‐NPs were further characterised by nanoparticle tracking analysis (NTA), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), X‐ray diffraction and Zetasizer. Moreover, nanoparticles containing nail paint (nanopaint) was formulated and its antifungal activity was also assessed against T. mentagrophytes and M. canis. ZnO‐NPs and formulated nanopaint containing ZnO‐NPs, both showed significant antifungal activity. The maximum activity was noted against M. canis and lesser against T. mentagrophytes. Minimum inhibitory concentration of ZnO‐NPs was also determined against the dermatophytes causing onychomycosis infection. Conclusion: ZnO‐NPs can be utilised as a potential antifungal agent for the treatment of onychomycosis after more experimental trials.Inspec keywords: diseases, zinc compounds, nanoparticles, nanofabrication, antibacterial activity, microorganisms, nanomedicine, ultraviolet spectra, visible spectra, Fourier transform infrared spectra, transmission electron microscopy, X‐ray diffraction, biomedical materials, patient treatmentOther keywords: zinc oxide nanoparticle biosynthesis, Rosa indica L petals extract, nail paint, antifungal activity evaluation, dermatophyte, Trichophyton mentagrophytes, Microsporum canis, antibiotics activity, ultraviolet‐visible spectroscopy, nanoparticle tracking analysis, Fourier transform infrared spectroscopy, transmission electron microscopy, X‐ray diffraction, zetasizer, antifungal agent, onychomycosis treatment     

Evaluation of antimicrobial activity of synthesised silver nanoparticles using Thymus kotschyanus aqueous extract

Development of a green chemistry process for the synthesis of silver nanoparticles (AgNPs) has become a focus of interest. Characteristics of AgNPs were determined using techniques, such as ultraviolet–visible spectroscopy (UV–vis), Fourier transform infrared (FTIR) analysis, scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy and X‐ray diffraction (XRD). The synthesised AgNPs using Thymus kotschyanus had the most growth inhibition against gram‐positive bacteria such as Staphylococcus aureus and Bacillus subtilise, while the growth inhibition of AgNPs at 1000–500 µg/ml occurred against Klebsiella pneumonia and at 1000–250 µg/ml of AgNPs was observed against E. coli. The UV–vis absorption spectra confirmed the formation of the AgNPs with the characteristic peak at 415 nm and SEM micrograph acknowledged spherical particles in a nanosize range. FTIR measured the possible biomolecules that are responsible for stabilisation of AgNPs. XRD analysis exhibited the crystalline nature of AgNPs and showed face‐centred cubic structure. The synthesised AgNPs revealed significant antibacterial activity against gram‐positive bacteria.Inspec keywords: visible spectra, microorganisms, ultraviolet spectra, biomedical materials, nanofabrication, nanoparticles, X‐ray diffraction, scanning electron microscopy, molecular biophysics, X‐ray chemical analysis, nanomedicine, silver, antibacterial activity, Fourier transform infrared spectraOther keywords: green chemistry process, ultraviolet–visible spectroscopy, gram‐positive bacteria, silver nanoparticles, Thymus kotschyanus aqueous extract, UV–vis spectroscopy, Fourier transform infrared spectroscopy, FTIR analysis, scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, SEM micrograph, X‐ray diffraction, XRD, Staphylococcus aureus, Bacillus subtilise, Klebsiella pneumonia, E. coli, UV–vis absorption spectra, face‐centred cubic structure, antibacterial activity, antimicrobial activity, wavelength 415.0 nm, Ag     

Biogenic fabrication of CuNPs,Cu bioconjugates and in vitro assessment of antimicrobial and antioxidant activity

In the present study, the authors synthesised copper nanoparticles (CuNPs) by using extract of Zingiber officinale (ginger) and later the NPs were bioconjugated with nisin, which shows antimicrobial activity against food spoilage microorganisms. CuNPs and its bioconjugate were characterised by ultraviolet–vis spectroscopy, NP tracking analysis, Zetasizer, transmission electron microscopy analysis, X‐ray diffraction and Fourier transform infra‐red (FTIR) spectroscopy. Zeta potential of CuNPs and its bioconjugate were found to be very stable. They evaluated in vitro efficacy of CuNPs and its bioconjugate against selected food spoilage bacteria: namely, Staphylococcus aureus, Pseudomonas fluorescens, Listeria monocytogenes and fungi including Fusarium moniliforme and Aspergillus niger. Antimicrobial activity of CuNPs was found to be maximum against F. moniliforme (18 mm) and the least activity was noted against L. monocytogenes (13 mm). Antioxidant activity of CuNPs and ginger extract was performed by various methods such as total antioxidant capacity reducing power assay, 1‐1‐diphenyl‐2‐picryl‐hydrazyl free radical scavenging assay and hydrogen peroxide assay. Antioxidant activity of CuNPs was higher as compared with ginger extract. Hence, CuNPs and its bioconjugate can be used against food spoilage microorganisms.Inspec keywords: antibacterial activity, biomedical materials, copper, nanoparticles, nanofabrication, nanomedicine, microorganisms, ultraviolet spectra, visible spectra, electrokinetic effects, transmission electron microscopy, X‐ray diffraction, Fourier transform infrared spectra, free radical reactions, food technologyOther keywords: biogenic fabrication, Cu bioconjugates, in vitro assessment, antimicrobial activity, antioxidant activity, copper nanoparticles, Zingiber officinale, ultraviolet‐visible spectroscopy, NP tracking analysis, Zetasizer, transmission electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, FTIR, zeta‐potential, food spoilage bacteria, Staphylococcus aureus, Pseudomonas fluorescens, Listeria monocytogenes, fungi, Fusarium moniliforme, Aspergillus niger, F. moniliforme, L. monocytogenes, ginger extract, total antioxidant capacity, power assay, 1‐1‐diphenyl‐2‐picryl‐hydrazyl free radical scavenging assay, hydrogen peroxide assay, food spoilage microorganisms, Cu     

Green synthesis,characterisation and biological evaluation of plant‐based silver nanoparticles using Quercus semecarpifolia Smith aqueous leaf extract

The development of reliable and green methods for the fabrication of metallic nanoparticles (NPs) has many advantages in the field of nanotechnology. In this direction, the present work describes an eco‐friendly and cost‐effective protocol for the production of silver NPs (AgNPs) using an aqueous extract of Quercus semecarpifolia leaves. Different techniques were carried out for the characterisation of the synthesised AgNPs. The ultraviolet–visible spectroscopic analysis showed the highest absorbance peak at 430 nm. The particle size and structure were confirmed by scanning electron microscopy as well as transmission electron microscopy (TEM) analysis. From TEM imaging, it was revealed that the formed particles were spherical with an average size of 20–50 nm. The crystalline nature of the NPs was determined by X‐ray powder diffraction patterns. Thermogravimetry and differential thermal analysis were also evaluated by a temperature increment from 100 to 1000°C. Bio‐inspired synthesis of AgNPs was performed for their pharmacological evaluation in relation to the activities of the crude methanolic, n ‐hexane, chloroform, ethyl acetate, and aqueous extracts. Good cytotoxic activity was exhibited by the green‐synthesised AgNPs (77%). Furthermore, the AgNPs were found to exhibit significant antioxidant activity at 300 μg/ml (82%). The AgNPs also exhibited good phytotoxic potential (75%).Inspec keywords: scanning electron microscopy, toxicology, visible spectra, particle size, nanofabrication, nanomedicine, transmission electron microscopy, silver, ultraviolet spectra, differential thermal analysis, nanoparticles, X‐ray diffraction, botany, biochemistry, cellular biophysicsOther keywords: green synthesis, biological evaluation, plant‐based silver nanoparticles, reliable methods, metallic nanoparticles, eco‐friendly cost‐effective protocol, silver NPs, ultraviolet–visible spectroscopic analysis, highest absorbance peak, particle size, structure, transmission electron microscopy analysis, TEM imaging, crystalline nature, X‐ray powder diffraction patterns, differential thermal analysis, pharmacological evaluation, aqueous extracts, good cytotoxic activity, significant antioxidant activity, AgNPs exhibited good phytotoxic potential, bio‐inspired synthesis, Quercus semecarpifolia Smith aqueous leaf extract, scanning electron microscopy, thermogravimetry, crude methanolic, n‐hexane, chloroform, ethyl acetate, phytotoxic potential, haemagglutination activity, size 20.0 nm to 50.0 nm, wavelength 430.0 nm, temperature 100 degC to 1000 degC, Ag     

Effect of Ag‐NPs synthesised by Chamaemelum nobile extract on the inflammation and oxidative stress induced by carrageenan in mice paw

An environmentally friendly and rapid procedure was developed to synthesise silver nanoparticles (Ag‐NPs) by Chamaemelum nobile extract and to evaluate its in vivo anti‐inflammatory and antioxidant activities. The ultraviolet–visible absorption spectrum of the synthesised Ag‐NPs showed an absorbance peak at 422. The average size of spherical nanoparticles was 24 nm as revealed by transmission electron microscopy. Fourier transform infra‐red spectroscopy analysis supported the presence of biological active compounds involved in the reduction of Ag ion and X‐ray diffraction confirmed the crystalline structure of the metallic Ag. The anti‐inflammatory and antioxidant activity of the Ag‐NPs was investigated against carrageenan‐induced paw oedema in mice. The levels of malondialdehyde (MDA) and antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase and inflammatory cytokines tumour necrosis factor (TNF‐α), interferon gamma and interleukin (IL)‐6, IL‐1β were assessed in this respect. The results demonstrated that anti‐inflammatory activity of the Ag‐NPs might be due to the ability of the nanoparticles to reduce IL‐1β, IL‐6 and TNF‐α. Moreover, reduction of antioxidant enzymes along with an increase in MDA level shows that the anti‐inflammatory activity of the synthesised Ag‐NPs by C. nobile is attributed to its ameliorating effect on the oxidative damage.Inspec keywords: silver, nanoparticles, nanofabrication, ultraviolet spectra, visible spectra, particle size, transmission electron microscopy, Fourier transform infrared spectra, X‐ray diffraction, crystal structure, enzymes, molecular biophysics, tumours, biomedical materials, nanomedicineOther keywords: Chamaemelum nobile extract, oxidative stress, mice paw, silver nanoparticles, antiinflammatory activity, antioxidant activity, ultraviolet‐visible absorption spectrum, spherical nanoparticle size, transmission electron microscopy, Fourier transform infrared spectroscopy, biological active compounds, X‐ray diffraction, crystalline structure, carrageenan‐induced paw oedema, malondialdehyde, antioxidant enzymes, superoxide dismutase, catalase, glutathione peroxidase, inflammatory cytokines, tumour necrosis factor, interferon gamma, interleukin, IL‐1β, IL‐6, TNF‐α, MDA level, Ag