Green synthesis of CuO nanoparticles loaded on the seashell surface using Rumex crispus seeds extract and its catalytic applications for reduction of dyes

In this study, CuO nanoparticles supported on the seashell (CuO NPs/seashell) was prepared using Rumex crispus seeds extract as a chelating and capping agent. The prepared nanocomposite was characterised by Fourier transform infrared spectroscopy, X‐ray diffraction, field emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy and transmission electron microscopy. The particle size of CuO NPs on the seashell sheets was in the range of 8–60 nm. Catalytic ability of CuO NPs/seashell was investigated for the reduction of 4‐nitrophenol (4‐NP) and Congo red (CR). It was observed that catalyst can be easily recovered and reused several times without any significant loss of catalytic efficiency.Inspec keywords: nanocomposites, nanoparticles, catalysis, dyes, Fourier transform infrared spectra, X‐ray diffraction, field emission electron microscopy, scanning electron microscopy, X‐ray chemical analysis, transmission electron microscopy, particle size, copper compoundsOther keywords: CuO, size 8 nm to 60 nm, Congo red, 4‐nitrophenol, particle size, transmission electron microscopy, energy dispersive X‐ray spectroscopy, field emission scanning electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, nanocomposite, capping agent, chelating agent, dye reduction, catalytic application, Rumex crispus seeds extract, seashell surface, nanoparticles, green synthesis     

Synthesis,characterisation and anti‐tumour activity of biopolymer based platinum nanoparticles and 5‐fluorouracil loaded platinum nanoparticles

A facile and green synthesis of platinum nanoparticles [gum kondagogu platinum nanoparticles (GKPtNP)] using biopolymer‐ gum kondagogu was developed. The formation of GKPtNP was confirmed by ultraviolet (UV)–visible spectroscopy, scanning electron microscopy–energy dispersive X‐ray spectroscopy, transmission electron microscopy, X‐ray diffraction, Zeta potential, Fourier transform infrared, inductively coupled plasma mass spectroscopy. The formed GKPtNP are well dispersed, homogeneous with a size of 2–4 ± 0.50 nm, having a negative zeta potential (−46.1 mV) indicating good stability. 5‐Fluorouracil (5FU) was loaded onto the synthesised GKPtNP, which leads to the development of a new combination of nanomedicine (5FU–GKPtNP). The in vitro drug release studies of 5FU–GKPtNP in pH 7.4 showed a sustained release profile over a period of 120 min. Agrobacterium tumefaciens induced in vitro potato tumour bioassay was employed for screening the anti‐tumour potentials of GKPtNP, 5FU, and 5FU–GKPtNP. The experimental results suggested a complete tumour inhibition by 5FU–GKPtNP at a lower concentration than the GKPtNP and 5FU. Furthermore, the mechanism of anti‐tumour activity was assessed by their interactions with DNA using agarose gel electrophoresis and UV‐spectroscopic analysis. The electrophoresis results revealed that the 5FU–GKPtNP totally diminishes DNA and the UV‐spectroscopic analysis showed a hyperchromic effect with red shift indicating intercalation type of binding with DNA. Over all, the present study revealed that the combined exposure of the nanoformulation resulted in the enhanced anti‐tumour effect. Inspec keywords: nanoparticles, transmission electron microscopy, biomedical materials, tumours, ultraviolet spectra, DNA, drugs, electrophoresis, polymers, platinum, pH, drug delivery systems, biochemistry, X‐ray chemical analysis, microorganisms, molecular biophysics, electrokinetic effects, X‐ray diffraction, scanning electron microscopy, cancer, nanofabrication, visible spectra, nanomedicine, Fourier transform infrared spectra, materials preparationOther keywords: 5FU–GKPtNP, 5‐fluorouracil loaded platinum nanoparticles, gum kondagogu platinum nanoparticles, antitumour activity, scanning electron microscopy‐energy dispersive X‐ray spectroscopy, biopolymer‐based platinum nanoparticles, biopolymer‐based platinum nanoparticles, ultraviolet‐visible spectroscopy, UV‐visible spectroscopy, transmission electron microscopy, X‐ray diffraction, zeta potential, Fourier transform infrared spectroscopy, inductively coupled plasma mass spectroscopy, nanomedicine, in vitro drug release studies, sustained release profile, Agrobacterium tumefaciens, in vitro potato tumour bioassay, tumour inhibition, tumour activity, agarose gel electrophoresis, UV‐spectroscopic analysis, DNA, time 120.0 min, Pt     

Biogenic synthesis,characterisation and antifungal activity of gum kondagogu‐silver nano bio composite construct: assessment of its mode of action

The biogenic synthesis of silver nanoparticles was achieved by using gum kondagogu (Cochlospermum gossypium), a natural biopolymer (Gk‐AgNPs). Synthesised nanoparticles were characterised by using UV–visible spectroscopy, inductively coupled plasma‐atomic emission spectrometer, X‐ray diffraction, transmission electron microscope techniques. The silver nano particle size determined was found to be 3.6 ± 2.2 nm. The synthesised Gk‐AgNPs showed antifungal activity and exhibited minimum inhibitory concentration and minimal fungicidal concentration values ranging from 3.5 to 6.5 µg mL⁻¹ against Aspergillus parasiticus (NRRL‐2999) and Aspergillus flavus (NRRL‐6513). Scanning electron microscopy–energy dispersive spectroscopy analysis revealed morphological changes including deformation, shrunken and ruptured mycelium of the fungi. At the biochemical level, the mode of action revealed that there was an elevated level of reactive oxygen species, lipid peroxidation, superoxide dismutase, and catalase enzyme activity. Increased oxidative stress led to increased outer membrane damage, which was confirmed by the entry of N ‐phenyl naphthylamine to the phospholipid layer of outer membrane and higher levels of K⁺ release from the fungi treated with Gk‐AgNPs. This study explores the possible application of biogenic silver nanoparticles produced from gum kondagogu as potent antifungal agents. The potent antifungal activity of Gk‐AgNPs gives scope for its relevance in biomedical application and as a seed dressing material.Inspec keywords: antibacterial activity, nanocomposites, silver, nanofabrication, nanoparticles, biomedical materials, polymers, visible spectra, ultraviolet spectra, atomic emission spectroscopy, X‐ray diffraction, transmission electron microscopy, scanning electron microscopy, microorganisms, X‐ray chemical analysis, enzymes, lipid bilayers, biomembranes, biomechanics, nanomedicineOther keywords: antifungal activity, gum kondagogu‐silver nanobiocomposite, Cochlospermum gossypium, natural biopolymer, UV‐visible spectroscopy, inductively coupled plasma‐atomic emission spectrometer, X‐ray diffraction, transmission electron microscope, fungicidal concentration, Aspergillus parasiticus, Aspergillus flavus, scanning electron microscopy, SEM‐energy dispersive spectroscopy, fungi deformation, ruptured mycelium, reactive oxygen species, lipid peroxidation, superoxide dismutase, catalase enzyme activity, oxidative stress, membrane damage, N‐phenyl naphthylamine, phospholipid layer, potassium ion release, biogenic silver nanoparticle, antifungal agent, seed dressing material, Ag     

Synthesis and characterisation of novel biopolymer stabilised organic Pt‐nanocomposite: assessment of its antioxidant and antitumour properties

Green synthesis of organic Pt‐nanocomposite was accomplished using carboplatin as a precursor and novel biopolymer – gum kondagogu (GK) as a reducing agent. The synthesised GK stabilised organic Pt‐nanocomposite (GKCPt NC) was characterised by different analytical techniques such as ultraviolet–visible spectroscopy, nanoparticle analyser, scanning electron microscopy and energy dispersive X‐ray analysis, X‐ray diffraction (XRD), Fourier‐transform infrared spectroscopy, transmission electron microscopy (TEM), X‐ray photoelectron spectroscopy (XPS) and inductively coupled plasma optical emission spectrophotometer. The XRD pattern established the amorphous nature of GKCPt NC. TEM analysis revealed the homogeneous, monodisperse and spherical nature, with Pt metal size of 3.08 ± 0.62 nm. The binding energy at 71.2 and 74.6 eV show the presence of metallic platinum, Pt(0) confirmed by XPS studies. Further, in vitro radical scavenging and antitumour activity of GKCPt NC have been investigated. In comparison to GK and carboplatin, GKCPt NC showed superior 1, 1‐diphenyl‐2‐picrylhydrazyle activity of 87.82%, whereas 2, 2‐azinobis‐(3‐ethylbenzthinzoline‐6‐sulphonic acid) activity was 38.50%, respectively. In vitro studies of the antitumour property of GK, GKCPt NC and carboplatin were evaluated by potato disc tumour bioassay model. The efficacy of synthesised GKCPt NC concentration (IC₅₀) on tumour inhibition was found to be 2.04‐fold lower as compared to carboplatin. Overall, the synthesised GKCPt NC shows both antitumour and antioxidant properties when compared to the original drug – carboplatin and might have promising applications in cancer therapy.Inspec keywords: nanoparticles, tumours, ultraviolet spectra, drugs, free radical reactions, X‐ray photoelectron spectra, platinum, nanocomposites, X‐ray diffraction, visible spectra, X‐ray chemical analysis, nanofabrication, transmission electron microscopy, scanning electron microscopy, cancer, polymer structure, filled polymers, Fourier transform infrared spectra, binding energy, drug delivery systems, nanomedicineOther keywords: antioxidant properties, green synthesis, ultraviolet–visible spectroscopy, energy dispersive X‐ray analysis, X‐ray diffraction, Fourier‐transform infrared spectroscopy, transmission electron microscopy, inductively coupled plasma optical emission spectrophotometry, antitumour activity, carboplatin precursor, biopolymer gum kondagogu stabilised organic Pt‐nanocomposite, reducing agent, different analytical techniques, scanning electron microscopy, X‐ray photoelectron spectroscopy, homogeneous particles, binding energy, in vitro radical scavenging, 1,1‐diphenyl‐2‐picrylhydrazyle activity, 2, 2‐azinobis‐(3‐ethylbenzthinzoline‐6‐sulphonic acid) activity, tumour inhibition, Pt     

Synthesis,characterisation and bactericidal effect of ZnO nanoparticles via chemical and bio‐assisted (Silybum marianum in vitro plantlets and callus extract) methods: a comparative study

Currently, the evolution of green chemistry in the synthesis of nanoparticles (NPs) with the usage of plants has captivated a great response. In this study, in vitro plantlets and callus of Silybum marianum were exploited as a stabilising agent for the synthesis of zinc oxide (ZnO) NPs using zinc acetate and sodium hydroxide as a substitute for chemical method. The contemporary investigation defines the synthesis of ZnO NPs prepared by chemical and bio‐extract‐assisted methods. Characterisation techniques such as X‐ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive X‐ray were used to confirm the synthesis. Although chemical and bio‐assisted methods are suitable choices for NPs synthesis, the bio‐assisted green assembly is advantageous due to superior stability. Moreover, this report describes the antibacterial activity of the synthesised NPs against standard strains of Klebsiella pneumonia and Bacillus subtilis.Inspec keywords: zinc compounds, II‐VI semiconductors, wide band gap semiconductors, nanoparticles, nanofabrication, semiconductor growth, antibacterial activity, X‐ray diffraction, X‐ray chemical analysis, scanning electron microscopy, Fourier transform infrared spectra, nanobiotechnologyOther keywords: chemical methods, bio‐assisted methods, Silybum marianum in vitro plantlets methods, Silybum marianum in vitro callus extract methods, green chemistry, zinc oxide nanoparticles, sodium hydroxide, zinc acetate, X‐ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, energy dispersive X‐ray analysis, bio‐assisted green assembly, antibacterial activity, Klebsiella pneumonia, Bacillus subtilis, ZnO     

Role of Ribes khorassanicum in the biosynthesis of AgNPs and their antibacterial properties

Silver nanoparticles (AgNPs) have been biosynthesised through the extracts of Ribes khorassanicum fruits, which served as the reducing agents and capping agents. Biosynthesised AgNPs have been found to be ultraviolet–visible (UV–vis) absorption spectra since they have displayed one surface plasmon resonance peak at 438 nm, attesting the formation of spherical NPs. These particles have been characterised by UV–vis, field‐emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy analysis. The formation of AgNPs at 1.0 mM concentration of AgNO₃ has resulted in NPs that contained mean diameters in a range of 20–40 nm. The green‐synthesised AgNPs have demonstrated high antibacterial effect against pathogenic bacteria (i.e. Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa). Biosynthesising metal NPs through plant extracts can serve as the facile and eco‐friendly alternative for chemical and/or physical methods that are utilised for large‐scale nanometal fabrication in various medical and industrial applications.Inspec keywords: X‐ray diffraction, X‐ray chemical analysis, nanofabrication, surface plasmon resonance, nanoparticles, antibacterial activity, microorganisms, scanning electron microscopy, silver, nanomedicine, visible spectra, ultraviolet spectra, transmission electron microscopy, Fourier transform infrared spectra, field emission scanning electron microscopy, biomedical materialsOther keywords: antibacterial properties, silver nanoparticles, reducing agents, capping agents, surface plasmon resonance peak, spherical NPs, field‐emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, transmission electron microscopy analysis, plant extracts, ultraviolet‐visible absorption spectra, Fourier transform infrared spectroscopy, antibacterial effect, Ribes khorassanicum fruits, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, surface plasmon resonance, AgNO₃ , Ag     

Catalytic reduction of 2,4‐dinitrophenylhydrazine by cuttlebone supported Pd NPs prepared using Conium maculatum leaf extract

A facile and green process to synthesise cuttlebone supported palladium nanoparticles (Pd NPs/cuttlebone) is reported using Conium maculatum leaf extract and in the absence of chemical solvents and hazardous materials. The antioxidant content of the C. maculatum leaf extract played a significant role in converting Pd²⁺ ions to Pd NPs. Various techniques were used for the characterisation of the Pd NPs/cuttlebone such as field‐emission scanning electron microscopy, X‐ray diffraction, energy dispersive X‐ray spectroscopy, Fourier transform infrared and ultraviolet–visible spectroscopy. This Pd NPs/cuttlebone showed excellent catalytic activity in the reduction of 2,4‐dinitrophenylhydrazine to 2,4‐diaminophenylhydrazine by sodium borohydride as the source of hydrogen at ambient condition. The catalyst could be separated and recycled up to five cycles with no loss of its activity.Inspec keywords: catalysis, catalysts, chemical engineering, palladium, nanoparticles, field emission electron microscopy, scanning electron microscopy, X‐ray diffraction, X‐ray chemical analysis, sodium compounds, ultraviolet spectroscopy, visible spectroscopyOther keywords: catalytic reduction, 2,4‐dinitrophenylhydrazine, cuttlebone, Conium maculatum leaf extract, green process, palladium nanoparticles, antioxidant content, field‐emission scanning electron microscopy, X‐ray diffraction, energy dispersive X‐ray spectroscopy, Fourier transform infrared, ultraviolet–visible spectroscopy, 2,4‐diaminophenylhydrazine, sodium borohydride     

Biosynthesis of waste pistachio shell supported silver nanoparticles for the catalytic reduction processes

Silver nanoparticles (NPs) are immobilised on pistachio shell surface by Cichorium intybus L. leaves extract as an antioxidant media. The Fourier transform infrared spectra, X‐ray diffraction, field‐emission scanning electron microscopy equipped with energy‐dispersive X‐ray spectroscopy, and transmission electron microscope analyses confirmed the support of silver NPs on the pistachio shell (Ag NPs/pistachio shell). Ag NPs on the pistachio shell had a diameter basically in the 10–15 nm range. Reduction reactions of 4‐nitrophenol (4‐NP), and organic dyes at ambient condition were used in the investigation of the catalytic performance of the prepared catalyst. Through this research, the Ag NPs/pistachio shell shows a high activity and recyclability, and reusability without loss of its catalytic activity.Inspec keywords: transmission electron microscopy, nanoparticles, X‐ray diffraction, catalysis, nanofabrication, dyes, X‐ray chemical analysis, reduction (chemical), silver, catalysts, Fourier transform infrared spectra, field emission scanning electron microscopyOther keywords: waste pistachio shell, silver nanoparticles, catalytic reduction processes, pistachio shell surface, antioxidant media, infrared spectra, X‐ray diffraction, field‐emission scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, transmission electron microscope analyses, reduction reactions, catalytic performance, catalytic activity, Cichorium intybus L. leaves extract, size 10.0 nm to 15.0 nm, Ag     

Antibacterial and anticancer activity of biosynthesised CuO nanoparticles

The present investigation aims for the synthesis of copper oxide nanoparticles (CuO NPs) using Nilgirianthus ciliatus plant extract. The obtained CuO NPs were characterised by X‐ray diffraction, Fourier transform infrared spectrum, ultraviolet–visible spectroscopy, photoluminescence, scanning electron microscopy and transmission electron microscopy analysis. Significant bacterial activity was manifested by CuO nanoparticles against both Gram‐positive (Staphylococcus aureus and Staphylococcus mutans) and Gram‐negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The synthesised CuO NPs have good cytotoxicity against both human breast cancer cell line (MCF‐7) and lung cancer cell line (A549) with minimum cytotoxic effect on normal L929 (fibroblast) cell lines.Inspec keywords: microorganisms, ultraviolet spectra, nanomedicine, transmission electron microscopy, visible spectra, cellular biophysics, antibacterial activity, nanoparticles, X‐ray diffraction, lung, copper compounds, cancer, toxicology, biomedical materials, scanning electron microscopy, photoluminescence, Fourier transform infrared spectraOther keywords: antibacterial activity, anticancer activity, biosynthesised CuO nanoparticles, copper oxide nanoparticles, Nilgirianthus ciliatus plant, X‐ray diffraction, infrared spectrum, ultraviolet–visible spectroscopy, transmission electron microscopy analysis, bacterial activity, Gram‐negative bacteria, synthesised CuO NPs, human breast cancer cell line, Staphylococcus aureus, Staphylococcus mutans, CuO     

Green synthesis of copper oxide nanoparticles and mosquito larvicidal activity against dengue,zika and chikungunya causing vector Aedes aegypti

In the present study, high purity copper oxide nanoparticles (NPs) were synthesised using Tridax procumbens leaf extract. Green syntheses of nano‐mosquitocides rely on plant compounds as reducing and stabilising agents. Copper oxide NPs were characterised using X‐ray diffraction (XRD) analysis, Fourier transform infrared (FT‐IR), Field‐emission scanning electron microscopy with energy dispersive spectroscopy, Ultraviolet–visible spectrophotometry and fluorescence spectroscopy. XRD studies of the NPs indicate crystalline nature which was perfectly matching with a monoclinic structure of bulk CuO with an average crystallite size of 16 nm. Formation of copper oxide NPs was confirmed by FT‐IR studies and photoluminescence spectra with emission peaks at 331, 411 and 433 nm were assigned to a near‐band‐edge emission band of CuO in the UV, violet and blue region. Gas chromatography–mass spectrometry studies inferred the phytochemical constituents of the leaf extract. Larvicidal activity of synthesised NPs using T. procumbens leaf extract was tested against Aedes aegypti species (dengue, chikungunya, zika and yellow fever transmit vector).Inspec keywords: photoluminescence, spectrophotometry, thermal analysis, chromatography, nanoparticles, antibacterial activity, field emission electron microscopy, microorganisms, wide band gap semiconductors, scanning electron microscopy, X‐ray diffraction, copper compounds, ultraviolet spectra, nanofabrication, X‐ray chemical analysis, crystallites, visible spectra, field emission scanning electron microscopy, nanobiotechnology, semiconductor materials, semiconductor growth, fluorescence, mass spectraOther keywords: energy dispersive spectroscopy, ultraviolet–visual spectrophotometry, fluorescence spectroscopy, chikungunya, green synthesis, mosquito larvicidal activity, zika, X‐ray diffraction analysis, field‐emission scanning electron microscopy, XRD, gas chromatography–mass spectrometry, copper oxide nanoparticles, dengue, tridax procumben leaf extract, nanomosquitocides, FTIR, monoclinic structure, crystallite size, photoluminescence spectra, near‐band‐edge emission band, phytochemical constituents, Aedes aegypti species, yellow fever transmit vector, CuO     

Structural and physicochemical properties of Rheum emodi mediated Mg(OH)2 nanoparticles and their antibacterial and cytotoxic potential

In the present investigation, Rheum emodi roots extract mediated magnesium hydroxide nanoparticles [Mg(OH)₂ NPs] through the bio‐inspired experimental technique were synthesised. Mg(OH)₂ NPs were characterised by using various characterisation techniques such as field emission scanning electron microscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and ultraviolet–visible spectroscopy. The formation of Mg(OH)₂ NPs was confirmed by X‐ray diffraction. The structural analysis confirmed the hexagonal crystal symmetry of Mg(OH)₂ NPs with space group P‐3m1 and space group no. 164 using the Rietveld refinement technique. TEM micrographs illustrated the nano‐size formation of Mg(OH)₂ NPs of spherical shape and size ∼14.86 nm. With the aid of FTIR data, plant metabolites such as anthraquinones have been identified as a stabilising and reducing agent for the synthesis of biogenic Mg(OH)₂ NPs. The synthesised Mg(OH)₂ NPs showed antimicrobial and cytotoxic potential against Gram‐negative and Gram‐positive bacteria such as Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) and MDA‐MB‐231 human breast cancer cell lines.Inspec keywords: antibacterial activity, microorganisms, visible spectra, cancer, X‐ray diffraction, cellular biophysics, nanomedicine, ultraviolet spectra, nanoparticles, transmission electron microscopy, nanofabrication, field emission scanning electron microscopy, Fourier transform infrared spectra, particle size, magnesium compounds, space groups, toxicologyOther keywords: physicochemical properties, structural properties, Rheum emodi root extract mediated magnesium hydroxide nanoparticles, bio‐inspired experimental technique, field emission scanning electron microscopy, transmission electron microscopy, TEM, Fourier transform infrared spectroscopy, FTIR spectroscopy, ultraviolet‐visible spectroscopy, X‐ray diffraction, hexagonal crystal symmetry, space group P‐3m1, space group no. 164, Rietveld refinement technique, nanosize formation, plant metabolites, spherical shape, antibacterial potential, cytotoxic potential, reducing agent, anthraquinones, stabilising agent, Gram‐positive bacteria, Gram‐negative bacteria, Escherichia coli, Staphylococcus aureus, MDA‐MB‐231 human breast cancer cell lines, Mg(OH)₂      

Enhanced wound healing activity of Ag–ZnO composite NPs in Wistar Albino rats

In the present study, silver (Ag) and Ag–zinc oxide (ZnO) composite nanoparticles (NPs) were synthesised and studied their wound‐healing efficacy on rat model. Ultraviolet–visible spectroscopy of AgNPs displayed an intense surface plasmon (SP) resonance absorption at 450 nm. After the addition of aqueous Zn acetate solution, SP resonance band has shown at 413.2 nm indicating a distinct blue shift of about 37 nm. X‐ray diffraction analysis Ag–ZnO composite NPs displayed existence of two mixed sets of diffraction peaks, i.e. both Ag and ZnO, whereas AgNPs exhibited face‐centred cubic structures of metallic Ag. Scanning electron microscope (EM) and transmission EM analyses of Ag–ZnO composite NPs revealed the morphology to be monodispersed hexagonal and quasi‐hexagonal NPs with distribution of particle size of 20–40 nm. Furthermore, the authors investigated the wound‐healing properties of Ag–ZnO composite NPs in an animal model and found that rapid healing within 10 days when compared with pure AgNPs and standard drug dermazin.Inspec keywords: wounds, tissue engineering, biomedical materials, nanocomposites, nanofabrication, nanomedicine, silver, zinc compounds, II‐VI semiconductors, wide band gap semiconductors, ultraviolet spectra, visible spectra, nanoparticles, particle size, surface plasmon resonance, spectral line shift, X‐ray diffraction, scanning electron microscopy, transmission electron microscopyOther keywords: enhanced wound healing activity, Ag‐ZnO composite nanoparticles, Wistar Albino rats, wound‐healing efficacy, ultraviolet‐visible spectroscopy, intense surface plasmon resonance absorption, aqueous Zn acetate solution, SP resonance band, blue shift, X‐ray diffraction analysis, diffraction peaks, face‐centred cubic structures, scanning electron microscope, SEM, transmission electron microscope, TEM, monodispersed hexagonal nanoparticles, quasihexagonal nanoparticles, particle size, animal model, time 10 d, size 20 nm to 40 nm, Ag‐ZnO     

Preparation of silver iodide nanoparticles using laser ablation in liquid for antibacterial applications

In this study, the authors reported the first synthesis process of silver iodide (AgI) nanoparticles (NPs) by pulsed laser ablation of the AgI target in deionised distilled water. The optical and structural properties of AgI NPs were investigated by using UV–vis absorption, X‐ray diffraction, scanning electron microscope (SEM), energy dispersive X‐ray, Fourier transform infrared spectroscopy, and transmission electron microscope (TEM). The optical data showed the presence of plasmon peak at 434 nm and the optical bandgap was found to be 2.6 eV at room temperature. SEM results confirm the agglomeration and aggregation of synthesised AgI NPs. TEM investigation showed that AgI NPs have a spherical shape and the average particle size was around 20 nm. The particle size distribution was the Gaussian type. The results showed that the synthesised AgI NPs have antibacterial activities against both bacterial strains and the activities were more potent against gram‐negative bacteria.Inspec keywords: antibacterial activity, nanoparticles, X‐ray chemical analysis, particle size, transmission electron microscopy, X‐ray diffraction, nanofabrication, scanning electron microscopy, visible spectra, ultraviolet spectra, silver compounds, pulsed laser deposition, Fourier transform infrared spectra, optical constants, energy gap, aggregationOther keywords: synthesis process, pulsed laser ablation, AgI target, deionised distilled water, optical properties, structural properties, UV–vis absorption, X‐ray diffraction, transmission electron microscope, optical data, optical bandgap, antibacterial activities, silver iodide nanoparticles, energy dispersive X‐ray analysis, SEM, wavelength 434.0 nm, temperature 293 K to 298 K, AgI     

Biosynthesis of the CuO nanoparticles using Euphorbia Chamaesyce leaf extract and investigation of their catalytic activity for the reduction of 4‐nitrophenol

Through this study an eco‐friendly, simple, efficient, cheap and biocompatible approach to the biosynthesis and stabilisation of CuO nanoparticles (NPs) using the Euphorbia Chamaesyce leaf extract is presented. The CuO NPs were monitored and characterised by field emission scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier transformed infrared spectroscopy, transmission electron microscope and UV‐visible spectroscopy. The biosynthesised CuO NPs showed good catalytic activity for the reduction of 4‐nitrophenol (4‐NP) in water during 180 s and reused 4 times without considerable loss of activity.Inspec keywords: copper compounds, nanoparticles, nanofabrication, catalysis, reduction (chemical), field emission electron microscopy, X‐ray chemical analysis, Fourier transform infrared spectra, transmission electron microscopy, ultraviolet spectra, visible spectraOther keywords: biosynthesis, CuO nanoparticles, Euphorbia Chamaesyce leaf extract, catalytic activity, 4‐nitrophenol reduction, nanoparticle stabilisation, field emission scanning electron microscopy, energy dispersive X‐ray spectroscopy, Fourier transformed infrared spectroscopy, transmission electron microscope, UV‐visible spectroscopy, CuO     

Silver nanoparticles biologically synthesised using tea leaf extracts and their use for extension of fruit shelf life

The biosynthesis of nanoparticles (NPs) from plant extracts is important in nanotechnology because the employed methods are environmentally friendly and cost‐effective. In this study, silver NPs (AgNPs) were synthesised using Chinese tea (Oolong tea) extract. The effects of the relative content of the employed silver nitrate, the reaction temperature, the incubation time, and the tea‐to‐water ratio on the formation of the AgNPs were examined. The synthesised AgNPs were also analysed by UV–vis spectroscopy, dynamic light scattering, transmission electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, and thermo‐gravimetric analysis. The NPs were observed to be highly crystalline, approximately spherical, and 10–50 nm in diameter. They were also tested for their use in preserving the postharvest quality of cherry tomatoes, with good results obtained. The tea AgNP treatment was specifically found to reduce the weight loss of the tomatoes, as well as changes in their total soluble solids, vitamin C, and titratable acid contents. The findings of this study indicate that postharvest tea AgNP treatment affords a clean, safe, high‐quality, and environmentally friendly method for extending the shelf life of fruits.Inspec keywords: silver, nanoparticles, nanofabrication, ultraviolet spectra, visible spectra, light scattering, transmission electron microscopy, X‐ray diffraction, Fourier transform infrared spectra, thermal analysisOther keywords: silver nanoparticles, tea leaf extracts, fruit shelf life, Chinese tea extract, Oolong tea, silver nitrate, reaction temperature, incubation time, tea‐water ratio, UV‐vis spectroscopy, dynamic light scattering, transmission electron microscopy, X‐ray diffraction, Fourier transform infrared spectroscopy, thermo‐gravimetric analysis, cherry tomatoes, Ag     

Role of AgNPs in the enhancement of seed germination and its effect on plumule and radicle length of Pennisetum glaucum

The authors have investigated beneficial effects of 1 mM of silver nanoparticles (AgNPs) on agriculturally important plant Pennisetum glaucum (Bajara). The extracellular AgNPs were synthesised using Bacillus subtilis spizizenni and characterised using ultraviolet–visible absorption spectroscopy, Fourier transform infrared spectroscopy (FT‐IR) and transmission electron microscopy (TEM). Optical absorption spectrum showed characteristic peak of AgNPs at 423 nm. FT‐IR analysis of AgNPs showed peak at 3435 cm⁻¹, which indicates the presence of N–H group (primary, secondary amines and amides) on the surface of AgNPs. TEM studies indicate that synthesised AgNPs have average size of ∼2 nm. Energy dispersive X‐ray spectroscopy showed strong signal of Ag at 3 keV. Treatment of 1 mM AgNPs to the bajara seeds was found to be sufficient for excellent germination of seeds within 3 days. There was also significant increase in radicle and plumule length as compared with control bajara seeds according to statistical analysis by one‐way analysis of variance, followed by Tukey''s test. The percentage of AgNPs detected in root samples was 0.003% (by inductively coupled plasma atomic emission spectroscopy), which is negligible. There is still need to study the bioavailability and the type of interaction of AgNPs with plants, necessary for application in agriculture.Inspec keywords: transmission electron microscopy, ultraviolet spectra, scanning electron microscopy, nanofabrication, X‐ray diffraction, nanoparticles, visible spectra, silver, atomic emission spectroscopy, X‐ray chemical analysis, Fourier transform infrared spectra, statistical analysis, agricultureOther keywords: ultraviolet–visible absorption spectroscopy, transmission electron microscopy, Pennisetum glaucum, Bacillus subtilis spizizenni, energy dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy, optical absorption spectrum, plumule length, radicle length, silver nanoparticles, Tukey''s test, inductively coupled plasma atomic emission spectroscopy, statistical analysis, Bajara seeds, scanning electron microscopy, X‐ray diffraction, analysis of variance, electron volt energy 3.0 keV, time 3.0 d, Ag     

Facile phyto‐mediated synthesis of silver nanoparticles using Chinese winter jujube (Ziziphus jujuba Mill. cv. Dongzao) extract and their antibacterial/catalytic properties

The aqueous extract of Chinese winter jujube (Ziziphus jujuba Mill. cv. Dongzao) was used as reducing and capping agents for the synthesis of silver nanoparticles (AgNPs) for the first time. The resulting AgNPs were characterised by UV/Visible (UV–Vis) spectroscopy, atomic force microscope, transmission electron microscopy, selected area electron diffraction, X‐ray diffraction, scanning electron microscopy, energy‐dispersive X‐ray and Fourier transform infrared spectroscopy (FTIR). The colloidal solution of AgNPs gave a maximum UV–Vis absorbance at 446 nm. The synthesised nanoparticles were almost in the spherical shapes with an average size of 11.5 ± 4. 8 nm. FTIR spectra were applied to identify the functional groups which were possibly responsible for the conversion of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with the biomolecules in the extract. The biosynthesised AgNPs showed a remarkable catalytic activity at room temperature, and they also showed good antibacterial properties against Escherichia coli and Staphylococcus aureus.Inspec keywords: silver, nanoparticles, nanofabrication, antibacterial activity, biomedical materials, nanobiotechnology, scanning electron microscopy, X‐ray diffraction, transmission electron microscopy, ultraviolet spectra, visible spectra, X‐ray chemical analysis, Fourier transform infrared spectra, catalysisOther keywords: wavelength 446 nm, temperature 293 K to 298 K, Ag, Escherichia coli, Staphylococcus aureus, biomolecules, catalytic activity, metal ions, colloidal solution, FTIR spectra, UV‐vis absorbance, TEM, SEM, XRD, Fourier transform infrared spectroscopy, energy‐dispersive X‐ray spectroscopy, scanning electron microscopy, X‐ray diffraction, selected area electron diffraction, transmission electron microscopy, atomic force microscopy, UV‐visible spectroscopy, catalytic properties, antibacterial properties, Chinese winter jujube extract, silver nanoparticles, facile phyto‐mediated synthesis     

Biogenic synthesis of Au,Ag and Au–Ag alloy nanoparticles using Cannabis sativa leaf extract

Biogenic synthesis of gold (Au), silver (Ag) and bimetallic alloy Au–Ag nanoparticles (NPs) from aqueous solutions using Cannabis sativa as reducing and stabilising agent has been presented in this report. Formation of NPs was monitored using UV–visible spectroscopy. Morphology of the synthesised metallic and bimetallic NPs was investigated using X‐ray diffraction and scanning electron microscopy. Elemental composition and the surface chemical state of NPs were confirmed by energy dispersive X‐ray spectroscopy analysis. Fourier transform‐infrared spectroscopy was utilised to identify the possible biomolecules responsible for the reduction and stabilisation of the NPs. Biological applicability of biosynthesised NPs was tested against five bacterial strains namely Klebsiella pneumonia, Bacillus subtilis (B. subtilis), Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa (P. aeruginosa) and Leishmania major promastigotes. The results showed considerable antibacterial and anti‐leishmanial activity. The Au–Ag bimetallic NPs showed improved antibacterial activity against B. subtilis and P. aeruginosa as compared to Au and Ag alone, while maximum anti‐leishmanial activity was observed at 250 μg ml⁻¹ NP concentration. These results suggest that biosynthesised NPs can be used as potent antibiotic and anti‐leishmanial agents.Inspec keywords: silver, silver alloys, gold, gold alloys, nanoparticles, nanofabrication, reduction (chemical), ultraviolet spectra, visible spectra, X‐ray diffraction, scanning electron microscopy, X‐ray chemical analysis, Fourier transform infrared spectra, microorganisms, antibacterial activityOther keywords: biogenic synthesis, Cannabis sativa leaf extract, bimetallic alloy Au–Ag nanoparticles, aqueous solutions, reducing agent, stabilising agent, UV–visible spectroscopy, X‐ray diffraction, scanning electron microscopy, elemental composition, surface chemical state, energy dispersive X‐ray spectroscopy analysis, Fourier transform‐infrared spectroscopy, biomolecules, bacterial strains, Klebsiella pneumonia, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Leishmania major promastigotes, antibacterial activity, anti‐leishmanial activity, Ag, Au, AuAg     

Green synthesis of copper oxide nanoparticles using aqueous extract of Convolvulus percicus L. as reusable catalysts in cross‐ coupling reactions and their antibacterial activity

CuO nanoparticles (NPs) were prepared by Convolvulus percicus leaves extract as a reducing and stabilising agent. The green synthesised copper oxide NPs was characterised by transmission electron microscope, energy dispersive X‐Ray spectroscopy, X‐ray diffraction, Fourier transform infrared and ultraviolet‐visible analysis. The activities of the CuO NPs as catalyst were tested in the formation of C‐N and C‐O bonds. The N ‐arylated and O ‐arylated products of amides, N‐H heterocycles and phenols were obtained in excellent yields. Furthermore, the separation and recovery of copper oxide NPs was very simple, effective and economical. The recovered catalyst can be reused several times without significant loss of its catalytic activity. Moreover, the antibacterial activity of these NPs was tested against two human pathogenic microbes and showed significant antimicrobial activity against these pathogenic bacteria.Inspec keywords: copper compounds, nanoparticles, nanomedicine, antibacterial activity, biomedical materials, nanofabrication, microorganisms, catalysts, transmission electron microscopy, X‐ray chemical analysis, X‐ray diffraction, Fourier transform spectra, infrared spectra, ultraviolet spectra, visible spectra, catalysisOther keywords: green synthesis, copper oxide nanoparticles, Convolvulus percicus L. aqueous extract, reusable catalysts, cross‐coupling reactions, antibacterial activity, reducing agent, stabilising agent, transmission electron microscope, energy dispersive X‐ray spectroscopy, X‐ray diffraction, Fourier transform infrared spectra, ultraviolet‐visible spectra, C‐N bonds, C‐O bonds, N‐arylated products, O‐arylated products, amides, N‐H heterocycles, phenols, catalytic activity, human pathogenic microbes, antimicrobial activity, CuO     

In vitro germination and biochemical profiling of Brassica napus in response to biosynthesised zinc nanoparticles

With the progression of nanotechnology, the use of nanoparticles (NPs) in consumer products has increased dramatically and green synthesis is one of the cheapest and eco‐friendly methods to obtain non‐hazardous NPs. In the current research zinc (Zn) NPs synthesis was carried out by using the fresh and healthy leaves of Mentha arvensis L. followed by characterisation through ultraviolet (UV)–visible spectroscopy, X‐ray diffraction (XRD) and scanning electron microscopy (SEM). UV–visible spectroscopy confirmed the green synthesis of ZnNPs, while XRD confirmed the size of NPs, which was 30–70 nm. SEM shows that the shape of ZnNPs was irregular. The effects of green synthesised NPs on two different varieties of Brassica napus were evaluated. Exposure to ZnNPs (5, 15, and 25 mg/l⁻¹) caused a significant increase in root and shoot length of B. napus. The application of NPs significantly improved plant germination and triggered the production of secondary metabolite and antioxidant enzymes. ZnNPs showed a significant increase in chlorophyll, superoxide dismutase, total flavonoid content (TFC) and antioxidant enzymes while total phenolic content was decreased when TFC increased. Thus, it has been concluded from the current study that ZnNPs may possibly trigger the production of antioxidant enzymes and various biochemical compounds.Inspec keywords: zinc, nanoparticles, nanofabrication, ultraviolet spectra, visible spectra, X‐ray diffraction, scanning electron microscopy, particle size, enzymes, molecular biophysics, biochemistry, nanobiotechnology, botanyOther keywords: biochemical profiling, Brassica napus, biosynthesised zinc nanoparticles, nanotechnology, Mentha arvensis L, ultraviolet‐visible spectroscopy, X‐ray diffraction, Zn, biochemical compounds, total phenolic content, total flavonoid content, superoxide dismutase, chlorophyll, antioxidant enzymes, secondary metabolite, plant germination, green synthesis, SEM, scanning electron microscopy, XRD