Assignment of the dynamin-1 gene (DNM1) to human chromosome 9q34 by fluorescence in situ hybridization and somatic cell hybrid analysis

The dynamins are recently discovered GTP-binding proteins postulated to mediate the scission of clathrin-coated vesicles at the plasma membrane. Of the three known mammalian dynamins, dynamin-1 (DNM1) appears to be particularly important for the formation of synaptic vesicles at presynaptic nerve termini. To investigate the possibility that mutations in the DNM1 gene cause a human disease, we determined the chromosomal localization of human DNM1. We conclude from fluorescence in situ hybridization and from the analysis of somatic cell hybrids that the map position in 9q34. This region has syntenic homology with mouse chromosome 2p, in agreement with the map position of the mouse DNM1 gene [see accompanying article by Klocke et al. (1997, Genomics 41:290-292)]. We discuss the potential relevance of the human DNM1 localization to diseases that were mapped genetically to the same chromosomal region.     

Assignment1 of the bystin gene BYSL to human chromosome band 6p21.1 by in situ hybridization

It is a sad fact that even with liberal doses of curiosity, an interesting issue and enough time and money, many good research ideas fail to reach the stage of a completed project. Not all of us have the time to devote to the research methods courses and workshops that have sprung up around the country in the past few years. The Research Committee of the RACGP plans to help. This is the first article in a series that will provide the new GP researcher with guidelines on asking the right question, choosing the best method, and getting results published. The series aims to make research in general practice more accessible by demystifying the jargon, clarifying the statistics and explaining the rules of publication.