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1.
Mary E. McKillican 《Journal of the American Oil Chemists' Society》1964,41(8):554-557
The phospholipid-glycolipid mixture, isolated chromatographically from the “free” (hexane soluble) and “bound” (hexane insoluble,
water-saturated butanol extractable) lipid of wheat endosperm, was fractionated by column and thinlayer silicic acid chromatography.
The components were identified by hydrolysis followed by thin-layer or paper chromatography of the products. They included
a sterol-containing glycolipid, hitherto unreported in wheat. The fatty acid and sterol compositions of the phospholipidglycolipid
components were determined by gasliquid chromatography. Differences were found between varieties and between components.
Contribution No. 14 of the Food Research Institute, Canada Dept. of Agriculture, Ottawa, Can.
Presented at the AOCS Fall Meeting, Minneapolis, 1963. 相似文献
2.
The low temperature crystallization technique for the enrichment of “minor” components, such as sterols and sterol esters,
from vegetable oils was applied to low erucic acid rapeseed oils. The recovery of free sterols and sterol esters was estimated
by use of14C-cholesterol and14C-cholesterol oleate. 80% of the free sterols and 45% of the sterol esters were recovered in the liquid fraction, while in
two studies total recoveries were 95% and 99%, respectively. This technique showed some selectivity toward the sterol bound
fatty acids when compared to direct preparative thin layer chromatography (TLC) of the crude oil. Gas liquid chromatography
(GLC) analysis of the free and esterified sterols as TMS-derivatives showed very little selectivity in the enrichment procedure.
The fatty acid patterns of the sterol esters demonstrated, however, a preference in the liquid fraction for those sterol esters
which have a high linoleic and linolenic acid content. The content of free sterols was 0.3–0.4% and that of sterol esters
0.7–1.2% of the rapeseed oils in both winter and summer types of low erucic acid rapeseed (Brassica napus) when the lipid classes were isolated by direct preparative TLC of the oils. The free sterols in the seven cultivars or breeding
lines analyzed were composed of 44–55% sitosterol, 27–36% campesterol, 17–21% brassicasterol, and a trace of cholesterol.
The esterified sterols were 47–57% sitosterol, 36–44% campesterol, 6–9% brassicasterol, and traces of cholesterol and Δ5-avenasterol.
The fatty acid patterns of these esters were characterized by ca. 30% oleic acid and ca. 50% linoleic acid, whereas these
acids constitute 60% and 20%, respectively, of the total fatty acids in the oil. Little or no variation in sterol and sterol
ester patterns with locality within Sweden was observed for the one cultivar of summer rapeseed investigated by the low temperature
crystallization technique. 相似文献
3.
Analyses of free sterol, steryl ester and fatty acid components from yeast secretion mutants indicated that free and esterified
sterol remained relatively constant over a growth range of 24 C to 34 C. The saturated fatty acid components (16∶0 and 18∶0)
increased while the unsaturated fatty acids (16∶1 and 18∶1) decreased as the growth temperature increased. In secretory mutants,
fatty acid composition changes are more pronounced than in the wild-type strain. A shift toward increased saturated and decreased
unsaturated fatty acid was observed when cells were subjected to a 2-hr temperature upshift to 37 C. Steady-state fluorescence
anisotropy data indicated that modifications to the lipid component of yeast plasma membrane produced lipid thermotropic transitions
that were 3 C to 6 C higher in yeast cells subjected to thermal stress. 相似文献
4.
The fatty acid composition of oyster larvae at various stages, as well as of the algal diet, were determined by gas liquid
chromatography (GC). Saturated fatty acids are the major fatty acid components in all larval stages and account for 34–62%,
30–35% and 35–81% of the neutral, polar and total lipids of algal-fed larvae respectively. Weight percentage of saturated
fatty acid in “starved” larvae was consistently higher (63–81%) during the whole period. The total polyunsaturated fatty acids
were higher in the polar lipids than in the neutral lipids. The concentration of the ω3 fatty acids also was comparatively
higher in the polar lipids than in the neutral lipids. In the total and neutral lipid fractions, the weight percentage of
polyunsaturated and ω3 fatty acids was higher in the eyed than in the pre-eyed (pediveliger) larvae. Eicosapentaenoic acid
(20∶5ω3) and 22∶6ω3 were not detected in lipids of “starved” and young larvae. There was an accumulation of 20∶5ω3, 22∶6ω3,
and total ω3 fatty acids in the older larvae. Lipid classes were separated by thin layer chromatography (TLC). There was no
qualitative change in lipid composition during larval development, but a marked increased of triacylglycerol in larvae up
to the stage of maturation in algae-fed larvae.
Contribution number 1195 of the Virginia Institute of Marine Science, Gloucester Point, VA 23062 相似文献
5.
Normal phase high performance liquid chromatography methods are described for the separation of neutral lipid, fatty acid
and five phospholipid classes using spectrophotometric detection at 206 nm. Separations were accomplished in less than 10
min for each lipid class. A mobile phase consisting of hexane/methyltertiarybutylether/acetic acid (100∶5∶0.02) proved effective
in separating cholesteryl ester and triglyceride with recoveries of 100% for radiolabeled cholesteryl oleate and 98% for radiolabeled
triolein. Free fatty acid and cholesterol were separated by two different mobile phases. The first, hexane/methyltertiarybutylether/acetic
acid (70∶30∶0.02) effectively separated free fatty acids and cholesterol, but did not separate cholesterol from 1,2-diglyceride.
A mobile phase consisting of hexane/isopropanol/acetic acid (100∶2∶0.02) effectively separated free fatty acid, cholesterol,
1,2-diglyceride and 1,3-diglyceride. Recoveries of oleic acid and cholesterol were 100% and 97%, respectively. Five phospholipid
classes were separated using methylteriarybutylether/methanol/aqueous ammonium acetate (pH 8.6) (5∶8∶2) as the mobile phase.
The recoveries of phosphatidylinositol, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin and lysophosphatidylcholine
were each greater than 96%. 相似文献
6.
M. A. Ali M. A. Gafur M. S. Rahman G. M. Ahmed 《Journal of the American Oil Chemists' Society》1985,62(3):520-523
The kernels of 10 different mango varieties were extracted. The physico-chemical characteristics and lipid class composition
of fats were studied. The fat content of mango kernels grown under the soil and climatic conditions of Bangladesh varied from
7.1% to 10%, depending on the variety. The total lipid extracts were fractionated into lipid classes by a combination of column
and thin layer chromatography (TLC). The hydrocarbon and sterol esters varied from 0.3% to 0.7%, triglycerides from 55.6%
to 91.5%, partial glycerides from 2.3% to 4% and free sterol from 0.3% to 0.6%. Free fatty acids amounted to 3.0–37% as oleic;
glycolipids were 0.6–1.2% and phospholipids 0.11–0.8%. The fatty acid composition of triglyceride (TG) fractions was analyzed
by gas liquid chromatography (GLC). Palmitic acid varied from 7.9 molar % to 10.0 molar %, stearic from 38.2% to 40.2%, oleic
from 41.1% to 43.8%, linoleic from 6.0% to 7.6%, linolenic from 0.6% to 1.0% and arachidic acid from 1.7% to 2.6%. TLC revealed
the presence of lyso-phosphatidylcholine, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and phosphatidic
acid in the phospholipid fraction. 相似文献
7.
To examine whether dietary fat alters membrane lipid composition and peroxidation of polyunsaturated fatty acids in “non-proliferative”
and “proliferative” cells in the large intestine, Sprague-Dawley rats were fed diets providing a polyunsaturated-to-saturated
fatty acid ratio of 1.2 or 0.3 at a high or low level of fat intake for a 25-day period. Cell populations were isolated and
the effect of dietary fat on membrane polyunsaturated fatty acid content and peroxide levels was determined. Neither fat level
nor fatty acid composition of diet influenced total cholesterol, total phospholipids, and percentage of phospholipid classes
in membrane phospholipids. Feeding the high fat and/or high polyunsaturated-to-saturated fatty acid ratio diet increased polyunsaturated
fatty acid content of mucosal cell phospholipids. Increase in polyunsaturated fatty acid content was paralleled by a decrease
in the monounsaturated fatty acid content of mucosal cell phospholipids. Membrane content of total saturated fatty acids was
not significantly affected by diet. Variation in phospholipid fatty acid composition between “non-proliferative” and ”proliferative”
cells was observed. Lipid peroxide levels in mucosal cell lipid fractions were altered by dietary fat treatment. Animals fed
high fat diets, compared to groups fed low fat diets, exhibited higher membrane peroxide levels when results are expressed
as nmol/mg protein. Higher peroxide levels were observed in mucosal cells for rats fed high polyunsaturated-to-saturated fatty
acid ratio diets when results were expressed per nmol of phospholipid. It is concluded that changes in fat level and fatty
acid composition of the diet alters the mucosal cell membrane lipid composition in the rat large intestine and influences
susceptibility of mucosal cell lipid to peroxidation. Further research is required to delineate which dietary factors—fat
level, polyunsaturated-to-saturated fatty acid ratio, or both—have a primary influence on the degree of lipid peroxidation. 相似文献
8.
The effect of temperature on the lipid composition ofCephalosporium falciforme andCephalosporium kiliense, causative agents of maduromycosis, was investigated. The fungi were grown at 28.5 C and 37 C in a chemically defined medium.
The lipids were solvent extracted, purified on Sephadex, and separated into their component classes by silicic acid column
chromatography. Five lipid classes were found: (a) sterol esters, (b) triacylglycerides, (c) free fatty acids, (d) sterols,
and (e) phospholipids. Fatty acids were analyzed by gas liquid chromatography and phospholipids by thin layer chromatography.
Temperature induced changes of varying degrees occurred in both the fatty acid phospholipid fractions of each organism. 相似文献
9.
The lipid classes, fatty acids of total and individual lipids and sterols of Antarctic krill (Euphausia superba Dana) from two areas of the Antarctic Ocean were analyzed by thin layer chromatography (TLC), gas liquid chromatography (GLC)
and gas liquid chromatography/mass spectrometry (GLC/MS). Basic differences in the lipid composition of krill from the Scotia
Sea (caught in Dec. 1977) and krill from the Gerlache Strait (caught in Mar. 1981) were not observed. The main lipid classes
found were: phosphatidylcholine (PC) (33–36%), phosphatidylethanolamine (PE) (5–6%), triacylglycerol (TG) (33–40%), free fatty
acids (FFA) (8–16%) and sterols (1.4–1.7%). Wax esters and sterol esters were present only in traces. More than 50 fatty acids
could be identified using GLC/MS, the major ones being 14∶0, 16∶0, 16∶1(n−7), 18∶1(n−9), 18∶1(n−7), 20∶5(n−3) and 22∶6(n−3).
Phytanic acid was found in a concentration of 3% of total fatty acids. Short, medium-chain and hydroxy fatty acids (C≤10)
were not detectable. The sterol fraction consisted of cholesterol, desmosterol and 22-dehydrocholesterol. 相似文献
10.
Mary E. McKillican 《Journal of the American Oil Chemists' Society》1966,43(7):461-465
Seeds ofCrambe abyssinica C.D. 6619 and theBrassica napus varieties Golden and Zero-erucic were collected at different stages of maturity and the free lipid extracted with hexane.
The lipid thus obtained was separated into lipid classes by silicic acid column chromatography. The lipid classes were further
examined by thin-layer chromatography and the component fatty acids and sterols by gas-liquid chromatography.
The relative amounts of the lipid classes in crambe and both rape varieties varied as the seed matured and a period of great
change occurred about 10 days after fertilization. The greatest change was in triglycerides and phospholipids plus glycolipids.
Free fatty acids, present in immature seeds, has almost disappeared at maturity. The lipid classes of crambe and both types
of rape were in similar proportion at maturity. Differences in phospholipid and glycolipid composition were found between
crambe and rape and between immature and mature rape. The fatty acid composition differred between lipid classes and changed
with maturity. Changes in 18-carbon acids of Zero-erucic rape were concurrent with the development of erucic and eicosenoic
acids in Golden rape.
Contribution No. 36 of the Food Research Institute. Presented at the AOCS Meeting, Cincinnati, October 1965. 相似文献
11.
Separation of sterol esters from wax esters in the lipids of vernix caseosa and adult human skin surface was accomplished
by column chromatography on MgO. The fatty acids of the sterol esters and wax esters of both samples were separated into saturates
and monoenes, and examined in detail by gas liquid chromatography (GLC). The saturated fatty acids of the wax esters of vernix
caseosa and of adult human skin surface were remarkably similar. They ranged in chain length from at least C11 to C30, six skeletal types being present: straight even, straight odd, iso, anteiso, other monomethyl branched and dimethyl branched.
A large number of patterns of monoenes were observed, each pattern consisting of desaturation of a specific chain at Δ6 or
Δ9 plus its extension or degradation products. The mole per cent of the total Δ6 and Δ9 patterns of wax ester fatty acid monoenes
of vernix caseosa were 87% and 12%, respectively, and 98% and 1%, respectively, for adult human skin surface lipid. The sterol
ester fatty acids of vernix caseosa were much different from those of adult human skin surface: vernix caseosa saturates were
largely branched and of lengths greater than C18, whereas the saturates of adult human surface lipid resembled the wax ester fatty acids. Of the vernix caseosa monoene patterns,
the mole per cent was 30% Δ6 and 70% Δ9, whereas of the adult human skin surface sterol ester fatty acids 89% were Δ6 and
11% Δ9. Chain extension was particularly pronounced in the sterol ester fatty acid monoenes of vernix caseosa amounting to
7–8 C2 units in some cases. The fatty acids of the sterol esters of both vernix caseosa and adult human skin surface appear to be
derived from the sebaceous gland and from the keratinizing epidermis, but those of the wax esters are from the sebaceous glands
only. 相似文献
12.
The total lipids of eleven species of Myctophids caught at depths between 20 and 700 m in the northern Pacific Ocean were
analyzed using silicic acid column chromatography (lipid classes) and capillary gas chromatography (fatty acid and fatty alcohol
composition). The major components in the lipid classes were triacylglycerols or wax esters; triacylglycerols were the dominant
acyl neutral lipids (68.1–96.1%) in eight species, and wax esters were found as the dominant lipid (85.5–87.9%) in three species.
The major fatty acids and alcohols contained in the was esters of the three fishes were 18:1n–9, 20:1n–9, 20:1n–11, and 22:1n–11
for fatty acids, and 16:0, 18:1, 20:1, and 22:1 for fatty alcohols. Fatty acids in the triacylglycerols ranging from C14 to C22 were predominantly of even chain length. The major components were 16:0, 16:1n–7, 18:1n–9, 20:1n–11, 22:1n–11, 20:5n–3 (icosapentaenoic
acid), and 22:6n–3 (docosahexaenoic acid). In both the triacylglycerols and the wax esters, the major fatty components were
monoenoic acids and alcohols. It is suggested from the lipid chemistry of the Myctophids that they may prey on the same organisms
as the certain pelagic fishes such as saury and herring, because the large quantities of monoenoic fatty acids are similar
to those of saury, herring, and sprats whose lipids originate from their prey organisms such as zooplanktons which are rich
in monoenoic wax esters. 相似文献
13.
The detailed composition of cellular lipid of more than 23 species of yeast has been determined quantitatively by thinchrography
on quartz rods, a method previously used for estimating cellular lipids of seven species of yeast. That data was fortified
by neutral and phospholipid quantitations on 30 species of yeast cells. Most of the test organisms contained 7–15% total lipid
and 3–6% total phospholipid per dry cell weight, except for the extremely high accumulation of triglycerides in two species
ofLipomyces. Qualitatively, 30 species of yeast cells contained similar neutral lipid constituents (triglyceride, sterol ester, free
fatty acid, and free sterol) and polar lipid components (phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine,
phosphatidyl inositol, cardiolipin, and ceramide monohexoside) without minor constituents. Based on the quantitative composition
of neutral lipids, the 30 species of yeast were divided into two groups, the triglyceride predominant group and the sterol
derivative group. These groupings were fairly well overlapped from the standpoint of the distribution characteristics of fatty
acid. The relative polar lipid compositions also grossly resembled each other. Only one exception of polar lipid composition
in yeast cells was found inRhodotorula rubra species which contained phosphatidyl ethanolamine as the most abundant phospholipid. Fatty acid distribution patterns in
yeast cells consistently coincided with other reports concerning fatty acid composition of yeast cells. Correlation of lipid
composition and classification of yeasts are suggested and discussed.
A part of this investigation has been reported at the 14th conference of the Japan Oil Chemists' Society, Nagoya, Japan, October
1975. 相似文献
14.
Nestor R. Bottino 《Lipids》1978,13(1):18-23
The blubber, liver, and muscle of the Antarctic sei whale were analyzed for total lipid content, composition of lipid by classes
and positional distribution of fatty acids in individual lipids. The major glycerolipids (triglycerides, phosphatidylcholine,
and phosphatidylethanolamine) were fractionated by silver nitrate thin layer chromatography. The phospholipid fractions were
analyzed for fatty acid positional distribution. The whale stomach contained almost exclusively the amphipodParathemisto gaudichaudi. Its lipids were also studied and compared with the lipids of the body tissues. The results indicate that the stomach content
lipids are subjected to modifications before being deposited in the blubber, liver, and muscle. According to the silver nitrate
thin layer chromatographic studies, liver and blubber triglycerides resemble each other in their patterns of positional distribution
of fatty acids and in molecular species composition. The phospholipids of liver and blubber also exhibited closely related
fatty acid distribution patterns. In general, while the proportions of lipid classes and their predominant fatty acids varied
from tissue to tissue, the patterns according to which the lipids had been synthesized seemed to be common.
“...And beneath the effulgent Antarctic skies I have boarded the Argo-Navis, and joined the chase against the starry Cetus
far beyond the utmost stretch of Hydrus and the Flying Fish.” (1). 相似文献
15.
Two improved methods have been developed for preparation of fatty acid methyl esters (FAME) from major O-ester lipid classes in blood, i.e., cholesterol ester, triacylglycerol, and glycerophospholipids. The methods involve simple
operations, and use neither harmful solvents such as chloroform or benzene nor highly reactive volatile reagents such as acetyl
chloride. The FAME synthesis reaction proceeds under mild temperature conditions. The methods include (1) extraction of lipids
from 0.2 ml of blood with 0.2 ml of tert-butyl methyl ether and 0.1 ml of methanol, (2) separation of the total lipids into lipid classes using a solid-phase extraction
column or thin-layer chromatography, and (3) methanolysis of each lipid class at room temperature or at 45 °C. In all the
operations, solvent concentration is performed only once prior to gas–liquid chromatography (GC). No noticeable differences
in composition determined by GC have been found between FAME prepared by the present methods and those prepared by a conventional
method involving lipid extraction with chloroform/methanol. The mild reaction and simplified procedures of the present methods
enabled safe and reproducible analysis of the fatty acid compositions of the major ester-lipid classes in blood. 相似文献
16.
R. G. Ackman S. N. Hooper S. Epstein M. Kelleher 《Journal of the American Oil Chemists' Society》1972,49(6):378-382
A sample of barracudina, a small fish potentially available in large quantities off Nova Scotia, contained 17.7% body lipid
of which 10% was triglyceride and 85% wax ester. The triglyceride, of calculated iodine value 48, was unusually rich in 14:0
(25.8%), 18:0 (4.3%), 20:0 (1.19%), 22:0 (0.45%) and 24:0 (0.75%). The wax ester fatty acids had a calculated iodine value
of 126 and a “normal” marine oil fatty acid composition. The wax ester fatty alcohols contained 42.2% hexadecanol, 29.5% octadecenols,
8.2% eicosenols and 3.8% docosenols. Certain interrelationships between fatty alcohols and fatty acids are indicated by details
of composition. The potential exploitation of barracudina lipid as a substitute for sperm oil in some uses appears possible. 相似文献
17.
Nikolaus Weber Klaus Vosmann Kurt Aitzetmüller Christin Filipponi Horst Taraschewski 《Lipids》1994,29(6):421-427
The sterol composition of free sterol and steryl ester fractions of the fish parasiteParatenuisentis ambiguus was determined. In addition, the fatty acid composition of various neutral lipid classes, i.e., wax esters, steryl esters,
triacylglycerols and free fatty acids, as well as the composition of the 1-O-alkyl moieties of total ether glycerolipids of the parasite, were investigated. The results of these studies were compared
with those obtained on the intestinal tract tissue of its host, the eel (Anguilla anguilla). Cholesterol is the major sterol in bothP. ambiguus andA. anguilla. However, the sterols ofP. ambiguus contain high proportions (>20%) of other sterols, such as campesterol and various dehydrosterols. [e.g., 7-dehydrocholesterol
and cholesta-5,22(E)-dienol]. The presence of these minor sterols agrees with the known biotransformations of exogenous sterols in various helminths.
Considerable differences are found in the fatty acid composition of neutral lipid fractions, as well as the total lipid extract
from the endoparasite as compared to the host tissue. In particular, eicosapentaenoic acid (20∶5n−3), other polyunsaturated
fatty acids, such as 20∶4n−6, 22∶5n−3 and 22∶6n−3, as well as long-chain saturated fatty acids, such as 20∶0, are generally
enriched in the neutral lipid fractions of the parasite as compared to those of infected eel intestine. The analysis of ether
glycerolipids revealed that 1-O-hexadecyl (16∶0) and 1-O-hexadecenyl (16∶1) moieties were present in similar proportions in the ether lipids of bothP. ambiguus and eel intestine, whereas 1-O-octadecyl (18∶0) moieties are more prominent in the parasite and 1-O-octadecenyl (18∶1) moieties in the eel. The results of these studies show thatP. ambiguus has specific mechanisms for the regulation of the sterol and fatty acid composition of its neutral lipids.
Dedicated to Professor Helmut K. Mangold on the occasion of his 70th birthday. 相似文献
18.
One sample of canola seed (variety Tower) and five samples of screenings were commercially processed to yield first an “expeller
oil” and subsequently an “extractor oil” by the hexane extraction of the residue. The screening samples contained 25–50% intact
or broken canola seed. The balance included 21–31% weed seeds (especially lambsquarter and stinkweed), hulls, fragments of
the embryo, and chaff. All the oil samples were analyzed for sterol and fatty acid composition. The extractor screening samples
had slightly higher sterol contents than the corresponding expeller samples, while the Tower samples gave the lowest values.
The averages (in mg/g oil or extract) for the extractor screening samples were: brassicasterol, 1.0; campesterol, 4.1; and
β-sitosterol, 7.3. For expeller screening samples the average were: 0.9, 3.6 and 6.2 and for the Tower oils they were, respectively,
0.9, 3.8, 5.3 and 0.9, 3.5, 4.7. The fatty acid compositions of the screening samples for both extractor and expeller oils
were similar to that of the Tower oil except for the higher proportions of docosenoic acid (22:1) and eicosenoic acid (20:1)
and the more obvious presence of three C18 conjugated dienes totalling up to 0.6% of one screening oil sample. The docosenoic acid level (mainly erucic acid) ranged
from 3.0 to 7.0% for the extractor oils and from 2.5 to 8.0% for the expeller samples, compared to 0.1% for the two Tower
oils. The oil contents of the screenings ranged from 20 to 30%, and the fatty acids and sterols appear to be nutritionally
useful and innocuous in all respects.
Presented in part at the ISF/AOCS World Congress, New York, April–May 1980. 相似文献
19.
Lipid changes in maturing oil-bearing plants. III. Changes in lipid classes in flax and safflower oils 总被引:1,自引:1,他引:0
Seeds from Raja flax and Indian safflower were collected at increasing stages of maturity and the free lipid extracted from
them with hexane. The true lipid material obtained in this manner was separated into lipid classes by silicic acid column
chromatography using a diethyl ether-hexane gradient and methanol for the phospholipids. Thin-layer chromatography was used
to establish the homogeneity of each lipid class. The composition of the lipid classes was examined by a combination of gas-liquid,
silicic acid-impregnated paper and thin-layer chromatography.
With both flax and safflower, the relative amounts of the different lipid classes were shown to vary as the seed matured;
the phospholipids showed the greatest degree of change. Free fatty acid, mono- and diglycerides were not encountered; acidity,
when present, was not due to lipid material. Differences within lipid classes were also investigated.
Presented at AOCS meeting in Chicago, Ill., 1961.
Contribution No. 84 from the Genetics and Plant Breeding Research Institute, Research Branch, Canada Department of Agriculture,
Ottawa. 相似文献
20.
The potential effects of oil specimens related to cases of toxic oil syndrome (TOS) on the liver microsomal lipid composition
from guinea pigs were investigated. For four weeks, animals were fed diets supplemented with either “case oil” (oil related
to cases of TOS) or “control oil” (oil unrelated to cases of TOS), either previously heated or not. Results were compared
with those from guinea pigs fed the same diet with no oil. The administration of case oil produced changes in liver microsomal
lipid composition. Statistically significant differences were also found between heated case and heated control oils. The
cholesterol/phospholipid molar ratios and the major phospholipid class distribution were unaffected under these diet conditions.
However, increases in the relative contents of linoleic and arachidonic acids and, simultaneously, a reduction in palmitic
and palmitoleic acid levels were observed by diet effects. Heated oil administration decreased the saturated/unsaturated ratios
in all cases. Our data suggest that changes observed in the fatty acid composition are attributable to the free fatty acid
contents of administered oils. The toxic constituents of case oil seem to be able to alter the liver microsomal lipid composition. 相似文献