Is Kaposi's-sarcoma-associated herpesvirus detectable in semen of HIV-infected homosexual men?

We explored a possible route of transmission of Kaposi's-sarcoma-associated herpes virus (KSHV) with nested and unnested PCR techniques. We looked for KSHV DNA sequences in semen of HIV-positive homosexual men and HIV-negative healthy semen donors. With unnested primers we found KSHV sequences in 21 of 33 (64%) homosexual men and in none of 30 healthy donors. With a nested PCR assay, 30 of 33 (91%) specimens from the homosexual men and 7 of 30 (23%) specimens from healthy donors had detectable KSHV sequences. Over 5 years of follow-up, 13 of 30 KSHV-positive homosexual men (43%) developed KS compared with none of the 3 KSHV-negative homosexual men.     

Immunochemical differences between glucocorticoid receptors from corticoid-sensitive and -resistant malignant lymphocytes

We have explored the possibility of using antibodies against purified rat liver glucocorticoid receptors to study the immunochemical properties of glucocorticoid receptors from murine and human malignant lymphocytes. For this purpose, purified immune immunoglobulin G was covalently linked to Sepharose CL-4B. We then examined the ability of the affinity gel to recognize cytosolic [3H]triamcinolone acetonide-receptor complexes from the corticoid-sensitive (CS) and -resistant strains of mouse lymphoma P1798, from CS lymphocytes of patients with chronic lymphatic leukemia, and from a CS clone of human leukemic lymphoblasts in tissue culture (CH6). Mouse thymus was used as a source of glucocorticoid receptor from normal CS lymphocytes. Whereas the immunoaffinity column retained 70 to 84% of the 58- to 62-A (Stokes radius) [3H]triamcinolone acetonide-receptor complexes characteristic of the CS mouse and human lymphocytes, it failed to recognize the 27- to 28-A (Stokes radius) glucocorticoid receptor present in corticoid-resistant mouse lymphoma P1798 cells. Therefore, under appropriate experimental conditions, it was possible to demonstrate cross-reactivity between the antiserum against rat liver glucocorticoid receptor and the 58- to 62-A (Stokes radius) glucocorticoid receptor from species as diverse as mouse and humans.     

A longitudinal study of seroreactivity against Mycoplasma penetrans in HIV-infected homosexual men: association with disease progression

The soluble form of the vascular cell adhesion molecule-1 (VCAM-1) is detectable in human sera and is elevated in diabetic patients, with unknown clinical significance. In the present study, the relationship between serum soluble VCAM-1 and diabetic microvascular complications (retinopathy, nephropathy, and neuropathy) was evaluated in 95 Japanese patients with Type 2 diabetes mellitus (DM). Serum soluble VCAM-1 concentration was higher in patients with more advanced stages of retinopathy as well as nephropathy. There was a significant correlation between soluble VCAM-1 and log10 (urinary albumin excretion) in 69 patients with normal serum creatinine levels (r = 0.51, p < 0.0001) and a significant correlation between soluble VCAM-1 and log10 (serum creatinine) in all the patients (r = 0.83, p < 0.0001). Soluble VCAM-1 concentration was also elevated in patients with neuropathy. There was a significant correlation between soluble VCAM-1 concentration and the number of microvascular complications (r = 0.59, p < 0.0001). However, multivariate regression analysis revealed that only diabetic nephropathy, was associated with the soluble VCAM-1 concentration. The elevation of circulating VCAM-1 level in diabetic nephropathy may result from underlying systemic endothelial dysfunction, increased VCAM-1 production in damaged renal tubular or glomerular epithelial cells and/or decreased renal clearance of this molecule, depending on the stage of nephropathy.     

Circadian rhythm of insulin-induced hypoglycemia in man

Four healthy men were given an insulin tolerance test (0.1 IU/kg BW) six times in different days at the following clock hours: 0400 h, 0800h, 1200 h, 1600 h, 2000 h, and 2400 h, in random order. Four days separated two consecutive tests. The glucose disappearance rate (K value) was calculated for each test between 5 min and 25 min after iv injection of insulin. The statistical evaluation by Cosinor test showed a statistically significant circadian rhythm for the hypoglycemic action of exogenous insulin with a peak at 1030 h (95% condifence limits: 0830 to 1430 h).     

Increased prevalence of oral Candida albicans serotype B in homosexual men: a comparative and longitudinal study in HIV-infected and HIV-negative patients

Several investigators have shown a comparatively high prevalence of Candida albicans serotype B among HIV-infected individuals. We serotyped oral C. albicans strains from 50 HIV-infected homosexual men, 39 HIV-seronegative homosexual men and 40 clinical oral isolates of a control group. The prevalence of serotype B was significantly higher in homosexual men, regardless of HIV serostatus, than in the control subjects. We suggest that the reported high prevalence of serotype B among AIDS patients in Europe and the USA simply reflects the high proportion of homosexual men among HIV-infected patients. In 22 subjects, oral C. albicans isolates were obtained at two or more time points, up to 8 years apart. No change in serotype was observed over time. The serotype prevalences in HIV-infected patients with oral thrush or AIDS-defining illness were similar to the group of homosexual men as a whole, indicating that there is no serotype-related variation in pathogenicity.     

Circadian rhythm in the response of central 5-HT1A receptors to 8-OH-DPAT in rats

Professional practice changes in the operating room have led to the development of a new role for nurses--as surgical assistants. Essentially, the surgical assistant nurse helps ensure the patient's safety while providing technical and clinical assistance to the surgeon in such areas as exposing and manipulating tissues, suturing fascia and using various specialized instruments. The surgical assistant nurse function is being implemented through a pilot project at the Institut de cardiologie de Montréal. Developed by representatives of the nursing and surgical departments there, the project was approved by the Collège des médecins du Québec in April 1995 and subsequently by the institute's council of physicians, dentists and pharmacists. It has also gained recognition from the Ordre des infirmières et infirmiers du Québec (OIIQ). The pilot project incorporated a 186-hour training program, which was delivered from May to September 1995 according to three distinct learning modules: maintenance of a safe environment for the patient, clinical assistance and technical assistance. A tutorial approach to learning included individual and team work, simulation exercises, technical courses, lab work and practical training in the operating room. At the end of the training program, two surgical assistant nurses began practising in their new role. An evaluation of the project will be performed in September 1996.     

Elevated physical health risk among gay men who conceal their homosexual identity.

This study examined the incidence of infectious and neoplastic diseases among 222 HIV-seronegative gay men who participated in the Natural History of AIDS Psychosocial Study. Those who concealed the expression of their homosexual identity experienced a significantly higher incidence of cancer (odds ratio?=?3.18) and several infectious diseases (pneumonia, bronchitis, sinusitis, and tuberculosis, odds ratio?=?2.91) over a 5-year follow-up period. These effects could not be attributed to differences in age, ethnicity, socioeconomic status, repressive coping style, health-relevant behavioral patterns (e.g., drug use, exercise), anxiety, depression, or reporting biases (e.g., negative affectivity, social desirability). Results are interpreted in the context of previous data linking concealed homosexual identity to other physical health outcomes (e.g., HIV progression and psychosomatic symptomatology) and theories linking psychological inhibition to physical illness. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Police discourses of homosexual men in New Zealand

The present study examined the discourses of homosexuality in the police, using the discourse analytic method developed by Potter and Wetherell (1987). The study asked how police officers constructed gay men using their discourses and why gay men should or should not be employed by the police. Eight police officers were interviewed. Analysis revealed officers drew on three distinct discourses to construct gay men. The effeminate discourse associated homosexuals with effeminate behaviors. The deviant discourse associated homosexuals with behaviors that are morally and legally prescribed, and the discourse of conditional acceptance was used by officers to positively present, while voicing reservations about homosexuals. These discourses formed the linguistic resources officers used to construct types of homosexuals that were subsequently rejected as potential police officers. Several arguments were used to justify the conclusion that homosexuals were unsuited to the police. These related to the internal and external pressures impinging on the department with regard to the employment of homosexuals. The implications of the discourses are discussed with reference to gay men, the police, and the ideologies of heterosexism and gender.     

Physical and biochemical characteristics of homosexual men.

Compared the height, weight, obesity, muscularity linearity, biacromial-bi-iliac ratio, and muscle strength of 44 homosexual and 111 heterosexual men. Homosexuals had less subcutaneous fat and smaller muscle/bone development and were longer in proportion to bulk. Their shoulders were narrower in relation to pelvic width, and their muscle strength was less. Linearity and muscularity were related to childhood build and certain behaviors. Urinary metabolites and blood serum lipids were compared for smaller groups (27-28 homosexuals, 109-111 heterosexuals). The homosexuals had lower creatinine and 17-ketogenic steroid levels, lower androsterone-etiocholanolone ratio, and higher 11-keto-etiocholanolone. They also had lower triglycerides, phospholipids, cholesterol, and beta-lipoproteins. It is concluded that the data support the thesis of an unidentified common factor underlying physical and personality characteristics and homosexuality. (16 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Circadian rhythm of breath hydrogen in young women

PURPOSE: The retention forces of a newly developed compomer cement (Dyract Cem), a glass ionomer cement (Ketac Cem Aplicap), and a resin cement (F21) were examined. MATERIAL AND METHODS: Cemented cast gold crowns were removed along the path of insertion with a Zwick universal testing device. The impact of both a cured and a noncured additional bonding layer that were applied to the inner surface of the crowns was examined across the Dyract Cem group. RESULTS: The mean adhesive strength was measured at 2.36 +/- 0.69 N/mm2 in the Ketac Cem group, at 0.60 +/- 0.28 N/mm2 in the F21 group, and at 1.85 +/- 0.94 N/mm2 in the Dyract Cem group, respectively. The application of an additional bonding layer to the inner surface of the crowns did not significantly improve the retentive strength of Dyract Cem; the respective mean strengths were observed at 1.46 +/-0.33 N/mm2 for the uncured and at 1.70 +/- 0.76 N/mm2 for the cured bonding layers. CONCLUSIONS: Dyract Cem and Ketac Cem showed significantly higher retentive strengths than F21 (p < 0.001, Wilcoxon test, 5% level). No significant difference was found in bond strength between Ketac Cem and Dyract Cem.     

Circadian rhythm of intraocular pressure in the rat

In order to investigate the relationship between the bioactive conformation of a peptide and its set of thermodynamically accessible structures in solution, the conformational profile of the tetrapeptide Ac-Pro-Ala-Pro-Tyr-OH was characterized by computational methods. Search of the conformational space was performed within the molecular mechanics frame-work using the AMBER4.0 force field with an effective dielectric constant of 80. Unique structures of the peptide were compared with its bioactive conformation for the protein Streptomyces griseus Protease A, as taken from the crystal structure of the enzyme-peptide complex. The results show that the bound conformation is close to one of the unique conformations characterized in the conformational search of the isolated peptide. Moreover, the lowest energy minimum characterized in the conformational search exhibits large deviations when compared to the bound conformation of the crystal structure.     

Childhood parental relationships of homosexual men.

Administered a 27-item questionnaire adapted from I. Bieber, et al. (see 40:2) to 43 homosexual and 142 hetersexual men in order to determine whether certain items which Bieber, et al. had reported as differentiating homosexual and hetersexual males who were in psychotherapy would also differentiate similar groups who had never sought psychotherapy. Data were based on retrospective self-reports regarding childhood fears and activities, and interparental and parent-child relationships. In the Bieber study, each S's psychoanalyst completed the questionnaire, so that their data were based on analysts' reconstructions of Ss' early lives. Results from the present study were similar to Bieber's in revealing more "negative" features in the backgrounds of the homosexuals. Results were considered neither to support nor refute the Bieber conclusions regarding the etiological importance of parental relationships in homosexuality. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Perceptions of general practice among homosexual men

BACKGROUND: Primary care has an important role to play in the prevention and management of the human immunodeficiency virus (HIV). It has been suggested that homosexual men experience a variety of problems in relation to primary care. AIM: As part of a larger study, it was decided to examine the extent to which a sample of homosexually active men experienced difficulties in general practice and whether they consulted their general practitioner for problems related to HIV or the acquired immune deficiency syndrome (AIDS). METHOD: Homosexual men were recruited for interview in 1991-92 from a variety of sources including genitourinary clinics and homosexual organizations. RESULTS: Of 623 men registered with a general practitioner 44% had not informed their general practitioner of their sexual orientation and 44% of the 77 men who were HIV antibody positive, as confirmed by the study, had not informed their general practitioner of this fact. Men who viewed their practice as unsympathetic towards homosexual men were less likely to have informed their general practitioner of their sexual orientation or HIV status. The majority of men (87%) nevertheless viewed primary care as an appropriate source of HIV/AIDS advice. CONCLUSION: There is considerable scope for improvement in the acceptability of general practice to homosexual men.     

Circadian rhythm disorders, motivation and Alzheimer's disease

In Alzheimer's disease, when the breakdown of the sleep's circadian rythm is sudden, it reflects frequently a concomitant somatic or psychiatric disorder. If the trouble appears progressively, there is less pathological or disturbing behaviors in the beginning. Later, it is often associated with a loss of interest for the daily living activities (perte de motivation).     

Circadian rhythm of plasma corticosteroids in heroin dependent subjects

The circadian rhythm of corticosteroid secretion was investigated in six heroin dependent subjects by measuring plasma corticosteroids at 09.00 hours and 24.00 hours. All results were within the normal range and it seems unlikely that chronic heroin dependence affects the hypothalamic control of corticosteroid secretion.