共查询到20条相似文献,搜索用时 125 毫秒
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采用标准加入法定量,建立了甘蓝中同时测定5种常用有机磷(敌敌畏、甲基对硫磷、毒死蜱、马拉硫磷、对硫磷)的气相色谱方法.用乙腈振荡提取残留在样品中的5种有机磷农药,采用NPD检测器对其进行测定.所测定的5种有机磷在DB-1701毛细管柱上得到了很好的分离.重复性实验中相对标准偏差为2.7040%~3.5095%,检测限为0.005~0.01 mg/kg.实验室比对中,测定同一样品中不同质量浓度的多农残,单点回收率不稳定,误差较大,标准加入法消除了基质的影响,克服了上述定量不准确的问题,在0.05~1.5 mg/L范围内有良好的线性关系.验证实验中,相对误差范围为1.2975~3.2779. 相似文献
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采用气相色谱法,以p,p'-DDE为内标物,对马拉硫磷.水胺硫磷悬乳剂进行测定。两种有效成分马拉硫磷、水胺硫磷在同一条件下测定,方法回收率均在99.0%~100.6%之间,变异系数均小于0.40%。方法简便、快速、准确且重现性好。 相似文献
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[目的]为了研究有机磷化合物在体外对猪肾细胞损伤作用。[方法]选择敌敌畏和马拉硫磷作用猪肾细胞系PK15。MTT比色法测量2种有机磷化合物对细胞抑制作用,荧光染色和DNA ladder法检测它们对PK15的致凋亡作用。同时检测氧化相关因子超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和PON1基因m RNA水平。[结果]敌敌畏和马拉硫磷抑制PK15细胞的生长呈时间-剂量效应,并能诱导PK15产生较明显的凋亡现象,同时能使细胞中的超氧化物歧化酶活性下降、丙二醛水平上升。[结论]2种有机磷农药可使细胞的抗氧化能力下降,致使细胞中的氧自由基水平上升,可能是有机磷化合物致细胞凋亡、损伤的因素之一。 相似文献
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高效液相色谱法测定大米中的马拉硫磷 总被引:6,自引:0,他引:6
马拉硫磷是一种高效、低毒、残效期短、分解快的化学杀虫剂。目前测定马拉硫磷农药残留量的方法是气相色谱法,但分析结果受许多因素影响。本文采用高效液相色谱法,样品中残留的马拉硫磷经提取、分离、净化、浓缩后进样测定,结果令人满意。 相似文献
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采用气相色谱法同时测定氯氰·马拉硫磷乳油中两种组分的含量,以癸二酸二丁酯为内标物,HP-5石英毛细管色谱柱分离,FID检测。试验结果表明:该方法在一定质量浓度范围内成良好的线性关系,相关系数r氯氰=0.9999、r马拉硫磷=0.9998,氯氰和马拉硫磷的标准偏差分别为0.018、0.122,变异系数分别为0.756%、0.665%,平均回收率分别为99.5%、99.7%。 相似文献
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建立大米中对硫磷、马拉硫磷、杀螟硫磷三种有机磷农药残留量检测的气相色谱方法。大米粉碎后用乙腈提取,外标法色谱峰面积定量。在0.01~1.00μg/mL线性范围内,硫磷、马拉硫磷、杀螟硫磷三种有机磷农药标准曲线的相关系数在0.9998~0.9999,最低检出限均为0.005μg/mL,平均回收率在104%~111%,相对标准偏差均≤3.61%。该方法对大米中有机磷农药的提取效果好,净化完全,杂质干扰少,前处理操作简单,定量准确,适合谷物中多组分有机磷农药残留的同时测定,易于推广应用。 相似文献
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构筑了乙酰胆碱酯酶/Nafion/丝网印刷电极(AChE/Nafion/SCE)修饰电极,研究该修饰电极在羧酸二茂铁与羧酸二茂铁和氯化乙酰巯基胆碱混合溶液中的电化学行为,建立测定有机磷农药-马拉硫磷的计时电流法。该传感器与固体电极所制传感器相比,具有制备简单,灵敏度高,体积小,使用简单,酶膜稳定不易脱落,测量电流稳定,制造成本低等优点。 相似文献
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研究了气相色谱–质谱(GC–MS)和气相色谱–二级质谱联用技术(GC–MS–MS)同时测定甲胺磷,氧乐果,谷硫磷和速灭磷等10种有机磷农药的分析方法。用GC–MS–MS方法进行测试,选择目标化合物的特征离子作为母离子,排除了背景干扰离子的影响,为复杂样品中微量农药的定性分析提供了新的方法。 相似文献
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《Journal of Industrial and Engineering Chemistry》2014,20(6):4393-4400
In the present paper, Amberlyst-15 resin was used for the removal of malathion. Various experimental parameters such as effect of pH, contact time, resin dose, initial malathion concentration and temperature were studied to find the optimum conditions for malathion removal. The removal rate of malathion by Amberlyst-15 was rapid and equilibrium was established within 30 min. Kinetic studies showed better applicability for pseudo-second-order model and equilibrium data was best fitted to Freundlich isotherm model. The thermodynamic parameters showed that the adsorption of malathion was feasible, endothermic and spontaneous. The concentration of malathion was determined using ultra-performance liquid chromatography–tandem mass spectrometry. 相似文献
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We evaluated the sublethal effects of malathion treatment on the behavioral responses of male Asian corn borers, Ostrinia furnacalis (Guenée), to their sex pheromones. Doses of malathion causing 1–50% mortality were topically applied to Asian corn borer male moths. The survivors were tested for behavioral and electroantennogram (EAG) responses to their sex pheromones. Sublethal doses of malathion affected males’ ability to locate a pheromone source. In wind tunnel tests, male moths treated with sublethal doses of malathion took 2.5 to 12 times as long to take flight as control moths. Malathion-treated male moths were 60–96% less likely to locate a sex pheromone source than control moths. Multiple linear regression analysis revealed that the proportions of males performing each key stage were significantly correlated with the doses of malathion, but not with the time since treatment. Sensitivity and specificity of perception to pheromones in male moths were also disrupted by sublethal doses of malathion. The EAG responses of males treated with malathion decreased 0.13 mV on average compared with control moths. In addition, the ratios of pheromone components that elicited the largest responses shifted from approximately 5:5 to 1:9 (E/Z) and 9:1. We conclude that treatment with sublethal doses of malathion significantly reduced the likelihood that male Asian corn borers could successfully locate a normal female releasing sex pheromones. 相似文献