胶体金试纸条法快速检测纺织品中总铅和总镉含量

建立了一种基于铅、镉单克隆抗体的胶体金试纸条方法,用于快速检测纺织品中总铅、总镉含量,通过加标实验及对实际试样进行测试,探讨了试纸条的灵敏度、准确性和稳定性.结果表明:制备胶体金试纸条时,铅、镉单克隆抗体较佳用量分别为50,45μL;试纸条对纺织品中总铅及总镉含量的检出限分别达5.0 mg/kg和0.625 mg/kg...     

氰戊菊酯残留胶体金免疫层析试纸条研制

应用胶体金免疫层析技术研制出一种准确、快速、简便检测氰戊菊酯农药残留的试纸条.采用柠檬酸三钠还原法制备胶体金,标记氰戊菊酯单克隆抗体,以硝酸纤维素膜为载体,包被氰戊菊酯抗原作为检测线(T线),包被兔抗鼠IgG抗体作为质控线(C线),经过竞争反应后目测结果.实验结果表明,该快速检测试纸条100%抑制浓度为800 ng/m...     

胶体金免疫层析试纸条在食品安全检测中的研究进展

为了提高免疫检测的速度,简化操作步骤,建立了胶体金免疫层析技术.它是利用纤维膜的毛细作用,使待测物和金标物(抗原/抗体)沿膜表面向前运动,与检测区的捕获物特异结合而聚集显色.它既有免疫学方法的特异性,又有简便快速,无需仪器的优点.本文主要介绍了胶体金免疫层析技术的在食品安全检测中的应用,并对胶体金免疫层析试纸条技术的发...     

犬细粒棘球绦虫感染胶体金检测试纸条的制备

目的制备犬细粒棘球绦虫感染胶体金检测试纸条。方法采用柠檬酸三钠还原法制备胶体金颗粒,用于标记抗细粒棘球绦虫Ediag A864单克隆抗体2D12制作金标垫,兔抗细粒棘球绦虫Ediag A864多克隆抗体标记检测线(T线),羊抗鼠Ig标记质控线(C线),制备胶体金试纸条,并进行特异性、敏感性、重复性及稳定性试验。用优化的胶体金检测试纸条对96份犬粪样品(36份参照阳性样,60份临床样品)进行检测。结果制备的犬细粒棘球绦虫感染胶体金检测试纸条与犬蛔虫阳性粪及犬贾第虫阳性粪样品均无交叉反应;粪便稀释度为1∶4时仍可检出正确结果;3次检测参照阳性样本均为阳性;于不同温度(室温、4及37℃)下保存不同时间(1、2、3、4个月)的试纸条均可检出正确结果。96份犬粪便样品中,36份参照阳性样品检出率为97.2%(35/36),60份临床样品均为阴性。结论制备的试纸条具有良好的特异性及稳定性,可应用于临床样品检测及大规模流行病学调查。     

ELISA法与胶体金试纸条检测HBsAg差异分析

目的探究胶体金试纸条与ELISA法检测HBsAg临床应用价值。方法分别用胶体金试纸条与ELISA法检测360份标本中的HBsAg,并对阳性率进行比较,然后将强阳性的10份标本作倍比稀释,观察2种检测方法灵敏度的差异。结果2种方法的阳性率之间无显著差异,ELISA法灵敏度较胶体金试纸条灵敏度高。结论2种方法皆是检测HBsAg的良好方法,胶体金试纸条法操作简便、快速适用于急诊及健康人群的筛查,ELISA法适用于常规检测。     

牛奶中抗生素快速检测试纸的研制

制作了牛奶中抗生素的快速检测试纸。探讨了TTC浓度、菌液浓度、浸泡时间等条件对试纸检测结果的影响。实验结果证明,抗生素检测试纸制作的最佳条件为:将中速滤纸浸泡在2%浓度的TTC试剂中5 min,干燥30 min。应用本试纸测试市售散装牛奶和袋装牛奶中氯霉素的含量,并与国标法检测结果相比较基本一致。检测试纸具有简便、快速、成本低等优点。     

河南研发出抗生素快速检测试纸

河南省科学院日前研发出一种新型快速检测试纸条。在新型快速检测试纸条上滴几滴鲜牛奶,只需5～109钟就可方便直观地确定牛奶中是否有抗生素药物残留。     

呋喃唑酮代谢物单克隆抗体及其新型免疫层析检测试纸条的制备

目的制备呋喃唑酮代谢物AOZ单克隆抗体及其胶体金与荧光淬灭免疫层析试纸条。方法将AOZ衍生成CPAOZ后,与载体蛋白BSA偶联,制备完全抗原CPAOZ-BSA,经皮下多点注射免疫BALB/c小鼠,共5次,取小鼠脾细胞与SP2/0细胞融合,获得稳定分泌特异性抗体的杂交瘤细胞株。利用获得的抗CPAOZ单克隆抗体制备胶体金及荧光淬灭免疫层析试纸条,并对试纸条的灵敏度及特异性进行验证。结果制备的完全抗原经紫外扫描鉴定偶联成功。共获得3株能稳定分泌抗CPAOZ单克隆抗体的杂交瘤细胞株,其中效价和特异性最好的1株2H11针对CPAOZ的50%抑制质量浓度(IC50)为0.88 ppb。以该抗体制备的胶体金及荧光淬灭免疫层析试纸条的最低检测限分别为7.5和0.062 5 ppb;两种试纸条与其他3种硝基呋喃类代谢物CPAMOZ、CPAHD、CPSEM均不存在交叉反应。结论成功制备了抗CPAOZ单抗及其胶体金免疫层析和荧光淬灭免疫层析试纸条,两种试纸条均具有较高的灵敏度和较强的特异性,其中荧光淬灭免疫层析试纸条的灵敏度较胶体金免疫层析试纸条高约100倍,具有良好的应用前景。     

试纸法快速检测果蔬中敌敌畏含量的研究

基于比色法原理,以定量滤纸为纸基,以间苯二酚溶液为显色剂,研制出一种快速检测果蔬中有机磷农药敌敌畏的试纸。结果表明,此方法的检出限为12.5 mg/kg,检测时间仅需5 min。该试纸法具有成本低、耗时短、操作简单、携带方便等优点,适合于果蔬中敌敌畏残留的现场初步筛查。     

试纸法快速检测果蔬中敌敌畏含量的研究

基于比色法原理,以定量滤纸为纸基,以间苯二酚溶液为显色剂,研制出一种快速检测果蔬中有机磷农药敌敌畏的试纸。结果表明,此方法的检出限为12.5 mg/kg,检测时间仅需5 min。该试纸法具有成本低、耗时短、操作简单、携带方便等优点,适合于果蔬中敌敌畏残留的现场初步筛查。     

土壤中的硝态氮检测试纸的制备及应用

根据NO-2氧化碘离子为单质碘,而碘遇淀粉显色这一反应特性,通过改变模拟土壤浸提液的pH值、催化剂用量、反应时间等还原浸提液中的硝态氮,探索出制备淀粉碘化钾试纸的最佳条件,并制作出标准色阶谱图。实验以分光光度法为验证手段,试纸法测量的土壤硝态氮含量与仪器检测结果基本吻合,且其它共存离子对显色几乎没有影响。     

Uranyl ion-selective optical test strip

A novel, optical sensor, test strip has been developed for the spectrophotometric determination of trace amounts of uranyl ions, UO₂²⁺, based on immobilization of C.I. Mordant Blue 29 (Chromazurol S)/cetyl N,N,N-trimethyl ammonium bromide ion pair on a triacetyl cellulose membrane. Optimization of the sensor for the detection of low levels of uranyl ion is described. The test strip responded linearly to uranyl ions between 3.0 × 10^?7 and 6.0 × 10^?5 mol L^?1; the reproducibility of the sensor at a medium level of UO₂²⁺ activity was ±0.55%. The optical sensor can be regenerated using 0.01 mol L^?1 HCl or 0.01 mol L^?1 NaF solution after 10 min. The developed test strip was used in the determination of UO₂²⁺ in ground water samples.     

PEG-based hydrogel-coated test strip for on-site urea determination

The urea test strip was prepared using polyethyleneglycol based hydrogel, urease and commercial pH-indicator paper. The swelling behavior of hydrogel indicated the ability of the urea to penetrate into the matrix and allowing the test strip to respond in a short time. The color formed on the strip was distinguished by naked eyes in one minute at room temperature. The linear range for urea was 20–200?mg/dL. The assays have good reproducibility. Relative standard deviation values were found within the range of 3.37–12.95% and 1.68–9.54% for intra- and inter-day assays, respectively.     

化妆品防腐剂及其检测技术研究进展

主要阐述了化妆品防腐剂的种类及其检测技术。简要介绍了防腐剂的作用机理和微生物使化妆品变质的原因。对常用化妆品防腐剂的种类和检测技术,如高效液相色谱、气相色谱、薄层色谱法和分光光度法等进行了重点论述。     

Thiobarbituric acid test for detecting lipid peroxides

The thiobarbituric acid (TBA) test has been used in the field of medical science in recent years to detect lipid peroxides. In this case, it is necessary for hydroperoxides to be decomposed to secondary products during the reaction. When purified methyl linoleate and methyl linolenate monohydro-peroxides were used as the sample for the TBA test, they did not decompose entirely to secondary products, but did so completely when an iron catalyst (ferrous sulfate) was added. However, the iron catalyst also accelerated the autoxidation of coexisting unsaturated fatty acids. Therefore, the addition of antioxidants was required. Fifteen min of heating was sufficient to complete the reaction. With additions of catalyst and antioxidant to the TBA test, it may be possible to make useful distinctions between hydroperoxides and secondary products of lipid oxidation.     

Simple,rapid method for detecting phase transitions of lipids

A simple rapid method was worked out for studying the physical and structural state of lipids by measuring the refractive indices of a layered lipid film. Melting processes of n-paraffins and phase transitions of phospholipids and the lecithin-cholesterol system also can be followed as a function of temperature. In accordance with data from the relevant literature, the measured refractive indices show that cholesterol lowers the phase transition temperature of lecithin. By isorefraction curves, the most ordered structure of the layers is indicated to occur at the equimolar ratio of cholesterol and lecithin. The method may be applied to study the effect of lipid-soluble agents upon lipid structure and is applicable for routine investigations and industrial purposes as well.     

Modelling the rapid chloride permeability test

A computer model has been written to simulate the Rapid Chloride Permeability Test (RCPT) described in ASTM C1202. The model represents the key processes of diffusion and electromigration using standard equations but then maintains charge neutrality by modelling changes to the voltage distribution. This method enables the model to predict current-time transients similar to those recorded in experiments and it can then be used to obtain basic parameters such as diffusion coefficients for tested samples by optimising to the observed data. Experimental data showing a non-linear voltage distribution is presented together with model results which show that non-linearity has a significant effect on the current. Other predictions from the model are compared with published data and shown to give good agreement.     

Automation in urinalysis: evaluation of three urine test strip analysers

A clinical laboratory evaluation was conducted on the Clinitek Auto 2000, the Super Aution Analyzer and the Urotron RL9 for the determination of glucose, protein, pH, blood, ketone-bodies and bilirubin.Precision of the systems was tested using three commercial control urine materials, and reported as the percentage of times the instrument repeats a certain value. Good repeatability was obtained with all the instruments.Accuracy of the systems was evaluated by comparison with quantitative procedures, and to check agreement between methods yielding semi-quantitative and quantitative results, ranges of acceptability were defined, based on the criteria reported in a previous paper [2]. It was then found that 87.5 to 98.9% of results from the Urotron RL9 and the Clinitek Auto 2000 were acceptable. With the Super Aution Analyzer the level of agreement was apparently lower because of the higher number of concentration steps used by this instrument.     

测土施肥的技术方法

测土施肥是国家推广的施肥技术。介绍测土施肥技术方法,包括:土壤样品采集方法,样品缩分分析,确定土壤能提供的养分量及土壤养分校正系数,确定目标产量及肥料养分利用率,最终确定作物施肥量。并举实例计算某作物的适宜施肥量。