黑曲霉低聚异麦芽糖高产菌株的诱变选育

以黑曲霉GXM-3为出发菌株进行60Co-γ射线与紫外线照射复合诱变育种,通过苔盼兰平板筛选及摇瓶培养,获得42株转化木薯淀粉液化液生成低聚异麦芽糖浆能力较强的突变株。其中,突变株D-597的转化能力最强。其在3L发酵罐中发酵80h,糖浆产物中低聚异麦芽糖含量达到最高值169.4mg/mL,比出发菌株提高了37.2%,发酵周期则缩短了40h。突变株D-597在利用木薯淀粉生产低聚异麦芽糖方面具有一定的工业应用前景。     

黑曲霉低聚异麦芽糖高产菌株的诱变选育

以黑曲霉GXM-3为出发菌株进行60Co-γ射线与紫外线照射复合诱变育种,通过苔盼兰平板筛选及摇瓶培养,获得42株转化木薯淀粉液化液生成低聚异麦芽糖浆能力较强的突变株。其中,突变株D-597的转化能力最强。其在3L发酵罐中发酵80h,糖浆产物中低聚异麦芽糖含量达到最高值169.4mg/mL,比出发菌株提高了37.2%,发酵周期则缩短了40h。突变株D-597在利用木薯淀粉生产低聚异麦芽糖方面具有一定的工业应用前景。      

黑曲霉AnM1产葡萄糖酸及纯化低聚异麦芽糖

该文对黑曲霉AnM1摇瓶发酵产葡萄糖酸及利用黑曲霉AnM1纯化低聚异麦芽糖进行研究。根据葡萄糖酸生产强度和得率确定黑曲霉AnM1摇瓶发酵培养基。发酵后收集的黑曲霉AnM1菌体可重复利用产葡萄糖酸,重复利用3次后葡萄糖酸的生产强度仍在7 g/（L·h）以上,得率为1.02 g/g葡萄糖。利用黑曲霉AnM1菌体氧化低聚异麦芽糖反应液中的葡萄糖,可将低聚异麦芽糖含量提高至总糖量的90%以上,其中有效三糖（异麦芽糖、潘糖、异麦芽三糖）含量占总糖的60%以上。黑曲霉AnM1具有较好的葡萄糖酸生产能力,菌体可用于纯化低聚异麦芽糖并能重复使用,在葡萄糖酸和低聚异麦芽糖生产方面具有很好的应用前景。     

低聚异麦芽糖的生理功能研究

低聚异麦芽糖是一种天然的生理活性物质,具有明显的增殖双歧杆菌的作用。本文主要研究了低聚异麦芽糖的生理功能,为其开发应用提供科学的依据。     

响应面法优化黑曲霉发酵产低聚异麦芽糖培养基

采用全因子试验、最陡爬坡试验以及Box-Behnken试验设计对黑曲霉CU-1(Aspergillus niger CU-1)发酵生产低聚异麦芽糖培养基的主要成分进行优化。结果表明:最优培养基成分为:麸皮浸汁体积分数4%、玉米浆添加量19.67g/L、NaNO3添加量2.24g/L、木薯淀粉糖化液添加量250g/L,在该培养条件下,在3.6L发酵罐中进行验证,发酵液中异麦芽糖、潘糖和异麦芽三糖总产量达到37.4%,低聚异麦芽糖总产量高达83.1%,说明Box-Behnken试验设计法用于黑曲霉发酵生产低聚异麦芽糖培养基优化是可行的,数学模型的预测值与实验观察值相符。     

低聚异麦芽糖在面包中的应用

低聚异麦芽糖具有良好的理化性质．如溶解性好、耐酸、耐热、糊精含量低等，同时还具有促进腩道内有益菌群——双歧轩菌等的增殖、预防龋齿等生理功能，是一种集营养、保健、疗效于一身的功能性低聚糖。研究了商品低聚异麦芽糖的部分理化特性，如甜度、耐酸性、耐热性、可溶性固形物、pH值、水分活度等，并探讨了低聚异麦芽糖在面包中的应用用低泉异麦芽糖产品部分代替蔗糖，应用于面包生产中，不仅质量较好，而且可以生产出新的功能性食品．     

低聚异麦芽糖的应用开发

低聚异麦芽糖不仅具有某些糖类的性质，而且具有防龋齿、增殖双歧杆菌等特殊的保健功能，对其开发并在食品、调味品、饮料、冷饮中应用，有着广阔的市场前景。     

低聚异麦芽糖的发展趋势

自二十世纪90年代初起,欧美、日本等发达国家和地区对双歧因子的应用开发已十分广泛,新加坡、荷兰、丹麦、马来西亚、韩国等也对其应用展开深入的研究。人们对集“营养、保健、食疗”于一体的双歧因子类产品需求旺盛。例如日本年需低聚糖双     

低聚异麦芽糖的生产和发展应用研究

低聚异麦芽糖是功能性低聚糖的大宗产品。主要对低聚异麦芽糖的理化性质、生理功能、国内外研究进展,以及工业生产现状和应用前景进行了综述。     

低聚异麦芽糖的最新研究进展

低聚异麦芽糖除具有优良的甜味特性、保湿性、耐酸耐热性、低水分活度和防止淀粉类食品老化等物理特征外，还具有增殖肠道双歧杆菌，防病抗病，延年益寿等特殊功效，而被广泛用于食品、饮料、医药、保健、啤酒、白酒等诸多领域，具有十分广阔的应用前景^[1]。     

黑曲霉突变株9-1-D糖化酶糖化淀粉条件研究

研究了黑曲霉突变株9-1-D耐热糖化酶糖化淀粉的条件,结果表明,耐热糖化酶9-1-D的最适糖化温度为60℃,糖化液的DE值为94.83%.在65℃时糖化液的DE值仍可达到90%以上,达到相同DE值时,糖化时间较市售糖化酶缩短了15h.     

Continuous Synthesis of Glucoamylase by Immobilized Fungal Mycelium of Aspergillus niger

The extracellular glucoamylase enzyme (EC 3.2.1.3) was synthesized continuously by the immobilized mycelial fragments of A. niger. Of the several polymeric matrices attempted for immobilization k-carrageenan and alginate were found to be the most effective. However, the enzyme activity exhibited by the immobilized mycelia (I.M.) was 15-20% lower than that of free cells under batch conditions. The immobilized cells have retained nearly the same enzymatic activity (120IU/g of I.M.) for 6 repeated batches and thereafter decline in activity was noticed. An aerated packed bed reactor with I.M. was operated continuously for 360 h. The volumetric productivity of the reactor was 1600IU/L/h for 192 h and reduced to 25% in 360 h.     

蜡样芽孢杆菌XZ30-2发酵液对黑曲霉的控制及安全性评价

该研究测定了蜡样芽孢杆菌(Bacillus cereus) XZ30-2对黑曲霉的抑制活性,制备XZ30-2的发酵液并分析其稳定性,进一步研究发酵液冻干粉对小麦感染黑曲霉的防治效果,并开展发酵液的动物安全性评价.结果 表明:蜡样芽孢杆菌XZ30-2对黑曲霉有较强的抑制活性,拮抗带宽5.32 mm,发酵液抑菌率为54.3...     

Intensification of Amylase Synthesis with Aspergillus niger by Way of Multistage Mutagenization

Conidia of amylolytically active Aspergillus niger C strain were subjected to four-stage mutagenization, using different combinations of mutagens in each stage (stage I – UV irradiation + ethyleneimine, stage II – UV irradiation + nitrosomethylurea, stage III – UV irradiation + N-methyl-N′-nitro-N-nitrosoguanidine, stage IV – acryflavine). In all, 378 strains were isolated after mutagenization, which were initially evaluated for the total amylolytic activity by the method of test-tube microculture. Nine most active mutants were then tested in submerged culture determining the activity of glucoamylase and α-amylase in post-culture liquid. The activity of α-amylase increased from over 7 to nearly 31%, and that of glucoamylase from 4 to over 61%.     

Amylase Synthesis by Forced Heterocaryons of Aspergillus niger in Submerged Culture Conditions

From 43 auxotrophic mutants A. niger found earlier 21 forced heterocaryons were obtained. All were examined with respect to total amylolytic activity by the method of test-tube microculture, whereas 12 of them (with the highest indices of this activity) were also tested with regard to glucoamylase synthesis. In most cases the amylase activity of hererocaryons was much higher than that of their component strains of lower and frequently of a higher activity. A distinct increase of this activity was also shown by heterocaryons formed from auxotrophs, originating from them in relation to prototrophic parent cultures with a weak amylase activity. However, none of the heterocaryons equalled the level of amylase synthesis, reached by a highly active prototroph A. niger C in this respect.     

Synthesis of Glucoamylase by Somatic Diploids of Aspergillus niger in Submerged Culture Conditions

Out of twelve forced heterocaryons of A. niger, in nine cases somatic diploids were obtained comprising a total of 107 strains. All were initially examined with respect to general amylase activity in test-tube microcultures. Over 21% of all diploid recombinants reached a higher coefficient of amylase activity than the parent haploid highly active in this respect. Four most active diploids selected from this group were examined with regard to glucoamylase synthesis. Also in this case diploids were characterized by a certain predominance in their activity over the initial prototrophic haploid strain (maximally about 16%).     

黑曲霉SL2-111产果胶酯酶的纯化与性质

通过硫酸铵分级沉淀、DEAE-Sepharose Fast Flow阴离子交换柱层析、Sephacryl S-200分子筛柱层析3个步骤,从黑曲霉SL2-111发酵的果胶酶粗品中纯化出一种电泳纯的果胶酯酶。经SDS-PAGE测定其分子质量约为48.8ku。最适pH值和最适温度分别为5.8和60℃。在50℃以下,pH值5.8-6.2之间酶活力相对稳定。     

响应面法优化黑曲霉SH312-26-19产果胶酶发酵条件

在单因素试验的基础上,运用响应面法优化黑曲霉SH312-26-19产果胶酶的发酵条件,并与市售果胶酶进行产甲醇含量的对比试验。结果表明,黑曲霉最适发酵条件为培养基中麸皮添加量11 g,发酵时间4 d,发酵温度30 ℃。在此优化条件下,该黑曲霉产果胶酶活力达到（2 518.69±19.34） U/mL。该果胶酶在酶促反应体系中甲醇的生成量分别比市售果胶酶1和市售果胶酶2低17.9%和12.8%,显著低于市售果胶酶（P＜0.05）。