人促甲状腺激素生物素—亲和素系统免疫放射分析法的建立

报道将ＢＡＳ引入双们点夹心ＩＲＭＡ，建立了人血清ＴＳＨ固相试管双位点夹心ＢＡＳ－ＩＲＭＡ。该法批内ＣＶ为１．４％－８．３％，批间ＣＶＯ ６．１％－８．４％，最小检出量为０．０４ｍＩＵ／Ｌ，平均回收率为１００．１６％，灵敏度约是同条件下ＩＲＭＡ的３倍；与ＩＲＭＡ对比测定２０份病人血管ＴＳＨ样品，经统计学处理，两者测定结果呈良好相关性。     

HCG免疫放射分析法的建立

采用两种抗ＨＣＶ单克隆抗体，固相试管包被技术建立了ＨＣＧ夹心免疫放射法，与人促黄体激素，促卵泡激素和促甲状腺激素均无交叉反应，方法的灵敏度为０．５ＩＵ／Ｌ，批内，批间变异系数分别小于５．４％，６．４％。药盒４℃下存放１个月质量稳定。     

血清3,5,3’-三碘甲腺原氨酸酶联免疫分析试剂盒的研制

以辣根过氧化物酶标记链亲合素(SA),T₃抗体包被微孔,T₃生物素化,四甲基联苯胺(TMB)为底物,建立了3,5,3’-三碘甲腺原氨酸（T₃）生物素-亲合素酶联免疫分析(T₃-BA-EL1SA)方法。结果显示,本方法分析灵敏度为0.2 μg/L,批内、批间变异系数分别为7.1％～8.3％和6.5％～10.5％,回收率为98.1％～107.2％;高浓度T₃血清样品系列倍比稀释后,测定值与稀释度呈线性相关,相关系数为0.995 7。本试剂盒操作简便、快速,适用于临床检测和科研应用。     

高灵敏度人促甲状腺激素(hTSH) 酶联免疫分析试剂盒

以辣根过氧化物酶标记链亲合素 (SA) ,二株识别人促甲状腺激素 (hTSH)高度特异的单克隆抗体分别用于包被微孔和生物素化 ,以四甲基联苯胺 (TMB)为底物 ,建立了高灵敏度人促甲状腺激素生物素 亲合素酶联免疫分析 (sTSH BA ELISA)试剂盒的制备方法。本试剂盒的灵敏度为 0 0 2mIU/L ,批内变异系数 <7 8% ,批间变异系数 <9 6% ,回收率为 93 3 %～ 1 0 7 8% ,特异性鉴定显示与其它糖蛋白激素 (如FSH ,HCG ,LH)无明显的交叉反应性。正常参考值范围为 0 3～ 4 1mIU/L。本法所制备的试剂盒与中国原子能科学研究院同位素研究所的TSH IRMA试剂盒的相关方程为 y =1 .2xIRMA 0 1 4,相关系数r =0 969。本试剂盒较常规ELISA灵敏度高、简便、快速 ,适用于临床检测和科研应用     

滤纸干血-新生儿促甲状腺素酶联免疫分析试剂盒的研制

以辣根过氧化物酶标记链亲合素(SA),两株识别人促甲状腺素(hTSH)高度特异的单克隆抗体分别用于包被微孔和生物素化,以四甲基联苯胺(TMB)为底物,建立了滤纸干血-新生儿促甲状腺素生物素-亲合素酶联免疫分析(NeonatalTSH-BA-ELISA)试剂盒的制备方法。本方法的灵敏度为0.5mIU/L,批内变异系数<15%,批间变异系数<20%,回收率为90.1%～103.3%。本方法所制备的试剂盒与NeonatalTSH-IR-MA(磁颗粒法)试剂盒具有良好的相关性,相关方程为y=1.01x+0.34,相关系数为r=0.873。本试剂盒无放射性,操作简便、快速,适用于临床检测和科研应用。     

用放射性受体结合分析法研究补肾填精中药对老年大鼠脑组织中神经递质受体的影响

用放射性受体结合分析法研究了补肾填精中药对雌、雄性老年大鼠脑组织中神经递质受体的影响。实验表明:雌、雄性老年大鼠大脑皮层组织中α_1和DA(多巴胺)受体的结合容量(R_1)有不同程度降低,补肾填精中药固真冲剂可明显增加老年大鼠大脑皮层组织中α_1和DA受体的R_1值,这一作用被认为可能对脑组织产生延缓衰老的作用。     

第三代电子动量谱仪的多参数符合测量系统研制

本文介绍了一种新研制的用于第３代电子动量谱仪的多参数符合测量系统。该系统以６个慢符合道的电荷脉冲信号（它含有位置信息）和一个快符合道中的符合时间信号作为７个参数，采用７个数据获到通道并行获取数据，经计算机系统处理，可同时得到（ｅ，２ｅ）反应相关的符合多道时间谱，电离能谱和电子动量谱。     

Discrete event dynamic system (DES)-based modeling for dynamic material flow in the pyroprocess

A modeling and simulation methodology was proposed in order to implement the dynamic material flow of the pyroprocess. Since the static mass balance provides the limited information on the material flow, it is hard to predict dynamic behavior according to event. Therefore, a discrete event system (DES)-based model named, PyroFlow, was developed at the Korea Atomic Energy Research Institute (KAERI). PyroFlow is able to calculate dynamic mass balance and also show various dynamic operational results in real time. By using PyroFlow, it is easy to rapidly predict unforeseeable results, such as throughput in unit process, accumulated product in buffer and operation status. As preliminary simulations, bottleneck analyses in the pyroprocess were carried out and consequently it was presented that operation strategy had influence on the productivity of the pyroprocess.     

Phébus-FP: Results and significance for plant safety in Switzerland

Phébus-FP is a multi-national collaborative programme comprising four integral and one debris bed melting and fission product release experiments. The integral experiments simulate the heat-up, degradation and fission product release and transport, thermal hydraulic response, aerosol behaviour and iodine chemistry in the containment. The total programme, including interpretation effort, runs from 1993 to ca. 2007. The experiments demonstrated new characteristics of core degradation, fission product and iodine chemistry and accident integral behaviour. PSI has participated extensively through planning support, interpretation of data from the experiments and analyses of the results to establish confidence in the models being used for plant application. A significant part of the PSI effort was performed to interpret the significance of silver iodide as an effective retention agent under typical reactor conditions. The conclusions are of an interim nature, since the data reduction and interpretation are not yet complete. The significance of the Phébus results for plant safety will be fully realised only after successful benchmarking of the computer codes against Phébus, and application to plant sequences. Such work remains in progress within the Phébus project and in various national programmes.