The phenolic profiles and antioxidant activity in different types of tea

Phenolics are suggested to be the major bioactive compounds responsible for the health benefits of tea. Seven types of tea were extracted using boiling water. The extraction kinetics was investigated. To more fully characterise the antioxidant profiles and possible associated health benefits of these tea types, the total water‐soluble solid content, phenolics, flavonoids, and antioxidant and antiproliferative activities were quantified. Green tea leaves (GTL) were found to have the highest phenolic content (128.7 ± 1.7 mg g^?1, P < 0.05), followed by teas of decaffeinated green tea bag (dGTB), green tea bag (GTB), black tea bag (BTB), decaffeinated BTB, black tea leaves and oolong tea leaves (OTL). Among the three leaf teas, GT displayed the greatest antioxidant activity (4850.2 ± 60.7 μmol g^?1, P < 0.05), followed by black tea (BT) and oolong tea (OT). As for the four bag teas, decaffeinated GT possessed the strongest antioxidant activity (4431.1 ± 335.0 μmol g^?1, P < 0.05), followed by GT, BT and decaffeinated BT. The results display that phenolic/flavonoid content well correlated with antioxidant activity. Proliferation of three human cancer cells was significantly inhibited in a dose‐dependent manner after exposure to some tea extracts. This may influence consumers in selecting the type of tea and tea brewing times, exhibiting greater health benefits.     

Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune

Antioxidant properties of hot water extract (HWE), hot water extracted polysaccharides (HWP) and hot alkali extracted polysaccharides (HWAE) were obtained from fruiting bodies of the wild basidiomycete Schizophyllum commune. All extracts contained both α- and β-glucans as determined by Megazyme β-glucan assay kit. Glucose was found by TLC and NMR to be the prevalent monosaccharide in all extracts. Total phenol contents were in descending order of HWP ≈ HWE > HWAE. Median effective concentrations (EC₅₀ values) of antioxidant activities were 8.3 ± 0.1, 6.9 ± 0.0 and 8.9 ± 0.1 mg/mL, and of the DPPH scavenging activity 0.8 ± 0.0, 0.6 ± 0.0 and 1.8 ± 0.0 mg/mL, for HWE, HWP and HWAE, respectively. EC₅₀ values of reducing power were 7.6 ± 0.1, 7.9 ± 0.0 and 12.5 ± 0.1 mg/mL, whereas those of the chelating abilities on ferrous ions were 3.1 ± 0.0, 4.6 ± 0.1 and 4.9 ± 0.1 mg/mL. The EC₅₀ values of the antioxidant activity, of the DPPH scavenging, and of the reducing power were correlated with total polysaccharide as well as with total phenol content. The antioxidant activities of all the extracts may be caused by both polysaccharides and polyphenols or by a complex of both.     

茶叶加工过程对咖啡叶化学成分和抗氧化活性的影响

本研究采用茶叶加工方法对咖啡叶进行加工,探索茶叶加工步骤（包括萎凋、揉捻、发酵、干燥）对不同叶龄咖啡叶的基础成分、植物化学成分和抗氧化活性的影响。结果表明,加工步骤和叶龄对基础成分、植物化学成分和抗氧化活性有不同程度的影响。咖啡叶中水浸出物的含量在整个加工步骤中总体呈下降趋势;可溶性糖的含量在前三个步骤中呈下降趋势,但是干燥后其含量有所上升;萎凋使咖啡幼叶中的游离氨基酸含量提高,然而从发酵步骤开始降低。咖啡因含量在幼叶中呈降低趋势但在成熟叶中变化不显著;芒果苷和芦丁含量在萎凋时升高,揉捻和发酵时降低,干燥时升高;绿原酸含量在发酵步骤结束之前呈下降趋势,干燥时又升高;总酚和总黄酮含量以及抗氧化活性的基本变化趋势与绿原酸相似。鲜咖啡幼叶的水分含量、可溶性糖、可溶性蛋白、植物化学成分和抗氧化活性均显著高于成熟叶且在相同步骤下其含量在幼叶与成熟叶中存在显著性差异。发酵2 h的基础成分含量、植物化学成分含量和抗氧化活性高于48 h。不同茶叶加工步骤对咖啡叶的植物化学成分和抗氧化活性具有不同的影响,其中发酵的时间对除了水分之外的基础成分和植物化学成分的影响最为显著。     

Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa

Partially purified polysaccharides (FP) and hot alkali extract (FNa) obtained from fruiting bodies of the wild basidiomycete Grifola frondosa were examined for their antimicrobial, antioxidant and cytotoxic activity. The structural properties of FP and FNa samples were investigated by FT-IR and high resolution 1H- and 13C-NMR spectroscopy. From a group of various G − and G + bacteria the antibacterial effects were highest against the G + B. cereus. FNa was the better antioxidant shown by the lower EC₅₀ values of DPPH scavenging ability, ferric-reducing antioxidant power and ferrous ion-chelating ability. Ferric-reducing antioxidant power and ferrous ion-chelating ability were mostly linked to total polysaccharides, total- and β-glucan content, as well as total protein content. Both extracts displayed a moderate dose dependent antiproliferative action towards malignant human breast cancer MDA-MB-453, cervical adenocarcinoma HeLa and myelogenous leukemia K562 cells not observed in the non cancer derived MRC-5 fibroblasts. The highest effect was found in HeLa cells for FP extract. The mean diameter of Ca-alginate bead loading FP was 960.7 μm while the mean diameter of beads encapsulating FNa extract was 1051.7 μm.     

3 种不同产地灵芝子实体主要化学成分比较

为探讨不同产地灵芝子实体（安徽1号、吉林1号和吉林2号）主要化学成分的差异,采用多种分析方法对其基本物质组成、微量元素、多糖、灵芝酸、氨基酸和脂肪酸进行分析。结果表明,不同产地灵芝子实体中蛋白质、氨基酸、脂肪酸、微量元素和三萜类化合物含量差异较大;3 种灵芝子实体中均有较高含量的Ca、Mg、Fe微量元素,以及较高含量的必需氨基酸和不饱和脂肪酸,氨基酸和脂肪酸总量由高到低依次均为安徽1号＞吉林2号＞吉林1号;3 种灵芝子实体水溶性粗多糖含量相近,但碱溶性粗多糖含量、纯化多糖的单糖组成差异较大;在3 种灵芝子实体样品中,安徽1号的灵芝酸A和灵芝酸B总含量（5.62 mg/g）最高。这些结论将为进一步完善灵芝子实体的质量标准提供技术支持。     

Infrared characterisation,monosaccharide profile and antioxidant activity of chemical fractionated polysaccharides from the edible seaweed sugar Kombu (Saccharina latissima)

The central goal of this study was to evaluate the antioxidant activities related to the nondigestible polysaccharides present in the brown alga Saccharina latissima. For this purpose, a chemical fractionation procedure based on the different solubilities of polysaccharides was achieved. FTIR spectra of freeze‐dried polysaccharide fractions were recorded to confirm the presence of sulphate. A significant correlation was found between sulphate content in the different fractions tested and antioxidant activities, either by reduction power (RP) or radical scavenging assay (RSA) (RP, P < 0.0001; and RSA‐ABTS P < 0.0001). The acidic fraction, with the highest sulphate content among soluble fractions and presumably containing sulphated fucans, showed the highest antioxidant activity either for reducing power or scavenging assays. Consistently, it is reported that the acid‐soluble fraction containing fucans shows higher antioxidant activity than those containing alginate and laminaran in Turbinaria conoides seaweed.     

Antioxidant and antiproliferative effects of methanol extracts from raw and fermented parts of mulberry plant (<Emphasis Type="Italic">Morus alba</Emphasis> L.)

Total phenolic content, phenolic acids, radical scavenging activity and antiproliferative properties of different parts of mulberry (Morus alba L.) were determined. The highest phenolic content was found in methanol extracts of mulberry root (117.7 ± 2.0), followed by leaves (71.4 ± 2.4), branches (49.0 ± 1.5) and fruit (11.2 ± 0.3) [mg ferulic acid equivalents (FAE) kg⁻¹ dry weight (DW)] (P < 0.05). The highest level of total phenolics in the fractions was in roots (166.2 ± 7.5 and 160.8 ± 7.2 mg kg⁻¹ DW for BuOH and EtOAC, respectively). Methanol extracts and their fractions dose dependently increased radical scavenging activity of mulberry branches, roots and leafs (more than 70%). Total phenolic content of the tested plant extracts was highly correlated with the radical scavenging activity. The antiproliferative effect of mulberry parts on human cell lines was different and connected to the concentrations of the investigated extracts. The fermentation of the mulberry leaves did increase their methanol extract antiproliferative effect only on human gastric carcinoma (SNU-601) cell line in concentration of 1,000 mg mL⁻¹. In conclusion, some plant parts of the Korean mulberry could be recommended as preventative and therapeutic agents, based on their antioxidant and antiproliferative properties.     

Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification

Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1 → 6)-β-d-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-γ inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by β-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-γ inducing activity in PBMCs fourfold.In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100 μg/mL the cells’ viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against ^•OH and ^•O2⁻ radicals of HWPE I and PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against ^•OH radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms.The good immunomodulating and antioxidative properties of A. brasiliensis polysaccharide extract obtained by hot water extraction and ethanol precipitation make it suitable for everyday use as an inexpensive dietary supplement.     

Optimization of a process to obtain selenium-enriched freeze-dried broccoli with high antioxidant properties

Broccoli exhibits a high content of antioxidant and anticarcinogenic compounds, such as polyphenols and selenium. Since it is mostly consumed as a processed food, elucidating the processing conditions that minimize nutritional losses becomes relevant. The process composed of a blanching step followed by freeze-drying of selenium-enriched broccoli was optimized, in order to maximize the content of Se-methyl selenocysteine (SMSeC), total polyphenols content and anti-radical power (ARP). The optimal blanching conditions agreed for the three responses, and were immersion in water at 72 °C during 1 min. The optimal freeze-drying conditions were slow freezing rate, atmospheric pressure (96.13 kPa), and application of infrared radiation (IR). These conditions allowed keeping 25% ARP, 18% polyphenols content and 174% SMSeC compared to the fresh vegetable. These results will help in establishing the most adequate processing conditions that allow keeping the functional properties of broccoli as a processed food.     

Bioactive substance contents and antioxidant capacity of raw and blanched vegetables

Five commonly consumed vegetables in Malaysia namely, four-angled bean (Psophocarpus tetragonolobus D.C.), French bean (Phaseolus vulgaris L.), long bean (Vigna sesquipedalis L.), snow pea (Pisum sativum var. macrocarpon L.) and snap pea (Pisum sativum) were blanched in boiling water for 10 min. The contents of total phenolics, ascorbic acid and β-carotene, and the antioxidant capacity as typified by β-carotene and free radical scavenging activity (DPPH) assays were determined for the raw and blanched vegetables. The study revealed that blanching caused a significant (p < 0.05) increase in β-carotene content [fresh (389–539 µg/100 g), blanched (510–818 µg/100 g)], except in snow pea. Conversely, there was a significant (p < 0.05) decrease in ascorbic acid content [fresh (1.2–7.8 mg/100 g), blanched (0.67–3.8 mg/100 g)]. After blanching, the total phenolic content and antioxidant activity either decreased or increased depending on the type of vegetables. The total phenolic content was positively correlated with the antioxidant activity of the studied vegetables to some extent, but not with ascorbic acid or β-carotene.Industrial relevanceBeans (four-angled bean, French bean and long bean), and peas (snow and snap pea) are commonly consumed vegetables in Malaysia. However, the scientific information regarding blanching and its effect on the total phenolic content and antioxidant activities is very limited. This work showed that blanching caused an increase in the carotene, total phenolic content and antioxidant activities in some of the selected vegetables. This work is important since, consumption of raw vegetables does not have high nutritional qualities and antioxidant activities as shown in this work.     

A comparative study of phenolic compounds and antioxidant and antiproliferative activities in frequently consumed raw vegetables

The aim of this study was to assess the antioxidant value and antiproliferative activity of some vegetables such as raw garlic (Allium sativum L), white and yellow, and red onions (Allium cepa L), red and green peppers (Capsicum annuum L.), and white cabbage (Brassica oleracea var. capitata f. alba), which were harvested in the same year and in the same geographical and climatic conditions. It was found that the highest content of some bioactive compounds (25.19 ± 2.2 mg GAE/g, 3.84 ± 0.3 mg CE/g, 4.88 ± 0.3 mg CE/g, 59.20 ± 0.8 μg CE/g, 1992 ± 99.8 μg AA/g, 452.7 ± 26.1 mg CGE/kg DW for polyphenols, flavonoids, tannins, flavanols, ascorbic acid, and anthocyanins, respectively) and the antioxidant activity (41.32 ± 3.9, 31.05 ± 2.7, 59.17 ± 5.2 and 58.94 ± 5.1 μM TE/g for DPPH, FRAP, CUPRAC and ABTS, respectively) was in red onion. Methanol extracts in concentration of 1,000 μg ml⁻¹ of garlic and red onion exhibited antiproliferative activity (83.1 ± 2.1 and 85.0 ± 3.2% of viability, respectively). In spite of relatively high antioxidant activity in methanol extracts of yellow onion, red and green pepper, no antiproliferative activity on both tumor cell lines was registered. In conclusion, among the studied vegetables raw red onion was the preferable. The interrelationship was in the following order: red onion > white onion = yellow onion > red pepper > garlic = green pepper > white cabbage. The antiproliferative activities of these vegetables were different: some samples reacted only on Calu-6 and the others—on SNU-601. Thus, vegetables from the same cultivation place were examined for their antioxidant and antiproliferative activities with four different methods. Based on obtained data a direct comparison between these vegetables was possible for the first time.     

Effect of water blanching on phenolic compounds,antioxidant activities,enzyme inactivation,microbial reduction,and surface structure of samnamul (Aruncus dioicus var kamtschaticus)

Water blanching is widely used in food industry, but imprudent process causes considerable quality deterioration. To determine optimum condition, the effect of water blanching at different temperature (80, 90 and 98 °C) and time (0–10 min) on the characteristics of samnamul was investigated. Total phenolic and flavonoid content and antioxidant activities showed dramatic increase up to maximum and decreased rapidly as further blanching prolonged at all temperatures. The blanching at 98 °C for 30 s showed the highest values of them. Phenolic compounds such as shanzhiside methyl ester, isoquercetin, quercetin 3-O-rhamnoside, kaempferol 3-O-glucoside and sergeolide showed different temperature dependence. At 30 s, quercetin derivatives levels increased with increasing temperature and showed highest heat-stability. Based on our study, the blanching at high temperature of 98 °C for a short time of 30 s may be important to increase or maintain nutritional quality, while oxidative enzyme activities and the microorganism numbers decreased.     

Traditional and industrial oven-dry processing of olive fruits: influence on textural properties,cell wall polysaccharide composition,and enzymatic activity

The preparation of table olives according to the Italian traditional “Ferrandina” method (Fer) includes an initial blanching step of black Cassanese olives, followed by salting and oven-drying. Its industrial implementation, also called the “Sybaris” method (Syb), replaces the blanching procedure by cutting the olives followed by immersion in water. The measurement of tensile properties showed that the Fer processing increased the weakness, softness, and deformability of the skin and the flesh of olive fruits, while the flesh of the Syb fruits became stronger and stiffer. These differences are probably correlated to the degradation and/or reorganisation of cell wall polysaccharides in the fruits. The degradation of pectic and hemicellulosic polysaccharides in the Fer olives was inferred by their increased solubility in aqueous solutions. Contrarily, retention of pectic polysaccharides was observed in Syb olives. As no correlation was found between cell wall degrading enzymatic activities and cell wall polysaccharides extractability, it is probable that these modifications were driven by heat.     

The stability of the casein–gluconate matrix in reduced‐lactose kefir with soluble fraction polysaccharides containing β‐glucan from Pleurotus ostreatus

In this experimental study, the soluble fraction of polysaccharides (SFP) was isolated from the fruiting bodies of Pleurotus ostreatus mushrooms, to stabilise the casein–gluconate kefir matrix. Soluble fraction of polysaccharides dissolved in water or in milk was added to the lactose‐reduced milk (0.5% w/v). Fermentation was carried out at 22 °C (to pH 4.4, 31°SH). Kefirs with SFP dissolved in the milk showed higher firmness, cohesiveness and consistency than the control kefir. The dissolving of SFP in the milk reduced spontaneous whey syneresis and prevented changes in water holding capacity. Soluble fraction of polysaccharides decreased the feeling of prickling in the kefirs and their mouth‐coating properties.     

Application of Analytical Methods for the Determination of Bioactive Compounds in Some Berries

Fluorometry, ESI-MS, FTIR, and radical scavenging assays were used for characterization of bioactive compounds and the levels of their antioxidant activities. Polyphenols, flavonoids, anthocyanins, and ascorbic acid and the level of antioxidant activity of water extracts of “Murtilla-like” [Myrteola nummularia (Poiret) Berg.], and other widely consumed berries were determined and compared. The contents of bioactive compounds and the levels of antioxidant activities in water extracts differed significantly in the investigated samples (P?<?0.05). “Murtilla-like” extracts contained polyphenols (mg GAE/g)—19.13?±?0.9, flavonoids, (mg CE/g)—3.12?±?0.1, anthocyanins (mg CGE/g)—120.23?±?5.4, and ascorbic acid (mg/g)—2.20?±?0.1; and antioxidant activities (μmolTE/g) by ABTS and CUPRAC assays were 200.55?±?8.7 and CUPRAC 116.76?±?5.7, respectively. Chemometrical processing was done on the basis of kinetic data of two variables (concentration and reaction time) by DPPH scavenging reaction. Polyphenol content highly correlated with antioxidant capacity (R² from 0.96 to 0.83). The quenching properties of berries were studied by the interaction of water polyphenol extracts with a small protein such as BSA by 3-D fluorescence and FTIR spectroscopy. These methods were used as additional tools for the characterization of polyphenols. Wild-grown non-investigated berries were compared with widely consumed ones, using their bioactive composition, antioxidant activities, and antiproliferative and fluorescence properties. In conclusion, the antioxidant properties of “Murtilla-like” can be used as a new source for consumption. The bioactivity of “Murtilla-like” is comparable with blueberries and raspberries. 3-D fluorescence and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of food products.

A comparative study of tocopherols composition and antioxidant properties of in vivo and in vitro ectomycorrhizal fungi

In aerobic organisms, the free radicals are constantly being produced during the normal cellular metabolism. The antioxidant properties of many organisms and particularly of wild mushrooms with their content in antioxidant compounds such as tocopherols, can detoxify potentially damaging forms of activated oxygen. Herein, a comparative study of tocopherols composition and antioxidant properties of in vivo (fruiting bodies) and in vitro (mycelia) ectomycorrhizal fungi: Paxillus involutus and Pisolithus arhizus. Tocopherols were determined by high performance liquid chromatography (HPLC) coupled to a fluorescence detector. The antioxidant properties were studied in terms of DPPH radical-scavenging activity, reducing power and inhibition of β-carotene bleaching. Fruiting bodies revealed the highest antioxidant properties, including scavenging effects on free radicals (EC₅₀ = 0.61 and 0.56 mg/ml) and inhibition of lipid peroxidation capacity (EC₅₀ = 0.40 and 0.24 mg/ml for P. involutus and P. arhizus, respectively), than mycelia produced in vitro cultures. Nevertheless, mycelia revealed higher levels of total tocopherols than fruiting bodies, and particularly P. arhizus mycelium proved to be a powerful source of γ-tocopherol (154.39 μg/g dry weight).     

江山白菇F21品系菇根的生化特性分析

以金属含量、氨基酸组成、差异表达蛋白以及抗氧化酶活性为指标对江山白菇F21品系菇根的生化组成进行了分析。结果表明：与菇身相比,菇根中粗脂肪、铁、磷以及必需氨基酸中的亮氨酸和赖氨酸含量较低,而灰分、砷、钙和镁元素含量较高;通过菇根与菇身的比较蛋白质组学分析,共鉴定出9 种差异表达蛋白,涉及双组分信号转导系统、乙酰辅酶A代谢、蛋白质合成、乙醛酸及二羧酸代谢、氮代谢多个生物学过程,同时菇根中的菇毒素含量显著低于菇身;货架期菇根中的抗氧化酶活性较低。     

Bioactives of fruiting bodies and submerged culture mycelia of Agaricus brasiliensis (A. blazei) and their antioxidant properties

Agaricus brasiliensis is a mushroom native from Brazil largely studied due to its polysaccharide contents, particularly β-glucans. In this study, the phenolics and organic acids contents as well as the antioxidant activities of its fruiting bodies and its mycelia obtained from submerged cultivation were compared. The hydroalcoholic extracts obtained from the fruiting bodies, early stationary mycelia and late stationary mycelia contain at least ten phenolic compounds and ten organic acids. Three phenolic compounds were identified as gallic acid, syringic acid and pyrogallol. Eight organic acids were identified as benzoic, oxalic, malic, acetic, alpha-ketoglutaric, citric, fumaric and trans-aconitic acids. All extracts presented antioxidant properties. The latter were evaluated by four assays: DPPH and ABTS radical scavenging activities, chelating ability for ferrous ions and inhibition of lipid peroxidation. The fruiting body extracts were more effective in the DPPH radical scavenging activity and lipid peroxidation inhibition that the mycelia extracts (P ≤ 0.05). The mycelia extracts were more effective in the ABTS radical scavenging activity and ferrous ion chelating ability (P ≤ 0.05). In conclusion, our results show that the mycelia of A. brasiliensis obtained in submerged cultivation can also be, as its fruiting bodies, valuable sources of antioxidant compounds.     

Grape juice obtained using steam extraction and other small‐scale extraction methods: phenolic content,antioxidant capacity and stability during storage

Grape juices made using small‐scale production techniques are widely consumed. The extraction procedures employed to produce them, however, can affect bioactive compounds and antioxidant activity in the final product. In this study, juices prepared using four extraction methods (steam, extractor, juicer and blender) were evaluated for soluble and hydrolysable polyphenol content, total anthocyanin content, antioxidant capacity, physicochemical characteristics and colour. Acceptance of steam‐extracted juices and their stability during storage were also evaluated. Steam extraction resulted in a higher soluble phenolic (1073 ± 58 mg gallic acid L^?1) and anthocyanin content (138 ± 22 mg cyanidin L^?1), as well as a higher antioxidant capacity when compared to juices prepared using other methods. Although steam‐extracted juice remained microbiologically stable during 24 months of storage, changes in phytochemical compounds and antioxidant capacity did occur. Our results indicate that steam‐extracted grape juices have high commercial potential.     

不同栽培基质桑黄化学成分及抗氧化活性比较

目的 以杨树桑黄Sanghuangporus vaninii为研究对象,比较分析了应用段木和木屑栽培的桑黄子实体中 水分、总灰分、酸不溶性灰分、浸出物、粗蛋白质、粗脂肪、粗纤维、总多糖、总黄酮、总三萜含量,麦角甾醇和麦角甾酮的含量差异,并研究了其乙醇提取物的体外抗氧化活性。方法 分别选取段木和木屑栽培的桑黄子实体,依据国家标准对其水分、总灰分、酸不溶性灰分、浸出物、粗蛋白质、粗脂肪、粗纤维的含量进行测定,通过紫外分光光度法、高效液相色谱法（HPLC）测定总多糖、总黄酮、总三萜、麦角甾醇、麦角甾酮含量,采用DPPH·自由基清除能力、ABTS+·自由基清除能力评价其乙醇提取物的抗氧化活性。结果 不同栽培基质对上述13个指标均具有影响。段木栽培的桑黄中水分、总灰分、酸不溶性灰分、浸出物、粗蛋白质、粗脂肪、粗纤维、总多糖、总三萜、麦角甾酮含量均高于木屑栽培桑黄;木屑栽培的桑黄中总黄酮、麦角甾醇含量高于段木栽培的桑黄;段木栽培桑黄的抗氧化活性高于木屑栽培桑黄。结论 段木培养基更适宜桑黄子实体中各种化学成分的积累,从而提高其抗氧化活性,代料栽培产桑黄子实体的培养基需进一步优化,研究结果为桑黄栽培基质的优化和桑黄饮品质量评价提供了科学的参考。