Influences of preharvest spraying Cryptococcus laurentii combined with postharvest chitosan coating on postharvest diseases and quality of table grapes in storage

The effects of preharvest spray with Cryptococcus laurentii combined with chitosan coating after harvest on decay and quality of table grapes during storage periods were evaluated in the present study. Preharvest spray with C. laurentii (PreA) significantly decreased decay index (DI), and postharvest chitosan coating (PCC) enhanced the effectiveness of the pre-harvest spray when fruits were stored at 0 °C. PreA combination with PCC increased the activities of polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) of fruit in storage. PreA + PCC treatment was effective in reducing weight loss of fruits by 85% at 17 d storage and 38% at 42 d storage as compared to PreA alone at the same stage. In addition, PreA enhanced the ratio of soluble solids content (SSC) to titratable acid (TA) by 12% at harvest time, 7% at 17 d storage and 25% at 42 d storage, mainly by increasing SSC and decreasing TA in fruit stored at 0 °C. These results suggested that integration of preharvest spray with C. laurentii and postharvest chitosan coating treatment may be a promising management strategy for decay control and quality maintenance of table grapes.     

Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage

The effects of preharvest chitosan spray (PCS) or/and postharvest chitosan coating (PCC) treatments on the quality and physiological response of table grape fruit stored at 20 or 0 °C was evaluated, respectively. PCS/PCC treatment showed the best control effect on decay. PCC or PCS/PCC treatment significantly decreased the weight loss of fruit stored at 20 °C. Additionally, all chitosan treatments inhibited the increase in rate of soluble solid content to titratable acid in fruit, stored at 20 °C, while enhancing the rate at 0 °C and affecting the content of total phenolic compounds in the fruit. Furthermore, the activities of superoxide dismutase decreased in all chitosan treatments and PCS or/and PCC treatments also changed the activities of polyphenol oxidase, peroxidase and phenylalanine ammonia-lyase. The results indicated the beneficial effect of chitosan by preharvest spray and/or postharvest coating on fruit quality and resistance to fruit decay.     

采前翠贝喷洒及采后壳聚糖涂膜对厚皮甜瓜低温贮藏期间冷害及品质的影响

厚皮甜瓜(Cucumis melo L.)对低温敏感,在低温条件下贮藏易发生冷害,导致果实品质下降,货架期缩短。本实验以\     

SO2对红地球葡萄的伤害及调控技术

本试验在１９９８－１９９９两年度进行,利用单一不同剂量的ＳＯ２、Ｓ昨配保鲜剂作为采前处理：１％的硝酸钙,不同浓度的果蔬液体保鲜剂、２倍的果亮、０．１％的壳聚糖对采收前的红地球葡萄喷布,干后采收贮藏作为采后处理,研究了不同处理对红地球葡萄漂白率及防腐保镁效果的影响。结果表明,红地球葡萄果皮对ＳＯ２最敏感,复配保镁剂通过减少ＳＯ２的使用剂降低果粒的漂白率：采前液体保鲜剂Ⅱ、果亮、壳聚糖则通过在葡萄表面     

采前纳他霉素处理对玫瑰香葡萄货架期品质及生理的影响

以玫瑰香葡萄为试材,采用不同浓度的纳他霉素进行采前浸果处理,研究常温(25℃)货架期内葡萄的贮藏品质及生理的变化。结果表明,质量浓度为0.5g/L的纳他霉素可以抑制采后葡萄果粒和果梗的霉烂,显著降低失重率,提高好果率,保持葡萄果实硬度,维持其低水平的呼吸强度,延长葡萄贮藏寿命,提高葡萄贮藏品质和安全性。     

Suppression of postharvest blue mould of apple fruit by Cryptococcus laurentii and N6‐benzyladenine

BACKGROUND: Cryptococcus laurentii is a well‐known postharvest yeast antagonist. N⁶‐benzyladenine (6‐BA), a cytokinin plant hormone, has a role in retarding ripening and senescence of harvested produce. This study was conducted to evaluate the efficacy of C. laurentii and 6‐BA in reducing the blue mould disease of apple fruit. RESULTS: The combination of C. laurentii with 6‐BA (20 µg mL^?1) was more effective in suppressing the Penicillium expansum infection in apple fruit wounds than C. laurentii alone, although 6‐BA (20 µg mL^?1) alone neither affected the growth of C. laurentii nor reduced the incidence of the blue mould disease in vivo. Moreover, treatment of apple fruit with C. laurentii and 6‐BA (20 µg mL^?1) resulted in stimulation of superoxide dismutase activity but in inhibition of the increase in peroxidase activity. CONCLUSION: 6‐BA (20 µg mL^?1) could enhance the efficacy of C. laurentii in reducing the postharvest blue mould disease of apple fruit, which offered great potential in minimizing the postharvest decay of apple fruit in an integrated pest management strategy. Copyright © 2008 Society of Chemical Industry     

Antifungal activity of chitosan and its application to control post‐harvest quality and fungal rotting of Tankan citrus fruit (Citrus tankan Hayata)

In this study, the antifungal activity of chitosan A (MW = 92.1 kDa) and B (MW = 357.3 kDa), with 94.2% N‐deacetylation were examined at various concentrations against fungi including Penicillium digitatum, Penicillium italicum, Botrydiplodia lecanidion and Botrytis cinerea. The effectiveness of these chitosans to control the post‐harvest quality of Tankan fruit, a popular sub‐tropical citrus fruit, was investigated. It was found that chitosan, depending on type and concentration, caused 25.0–90.5% growth inhibition on test organisms after 5 days of cultivation at 24 °C. Chitosan treatment significantly reduced (P < 0.05) the percentage decay of Tankan fruit during storage at 24 °C. After 42 days of storage at 13 °C, chitosan‐coated Tankan fruits were firmer, exhibited less decay and weight loss, and showed higher titratable acidity, ascorbic acid, and total soluble solids than the control fruit. Weight loss of Tankan fruits decreased as the concentration of chitosan was increased. On the other hand, chitosan A, regardless of concentration tested, was found to be more effective in retaining the firmness, titratable acidity and water content, total solid content and ascorbic acid content of Tankan fruits than chitosan B. Copyright © 2006 Society of Chemical Industry     

A combination of hot air treatment and nano‐packing reduces fruit decay and maintains quality in postharvest Chinese bayberries

BACKGOUND: Chinese bayberry is susceptible to microbial decay during postharvest handling and storage. To search for effective alternatives to currently used fungicides for disease control, the efficacy of hot air (HA) treatment at 48 °C for 3 h alone or in combination with nano‐packing (NP) containing nano‐TiO₂ and nano‐Ag in controlling green mould decay caused by Penicillium citrinum and natural decay, and their effects on fruit quality in harvested Chinese bayberries were investigated in this study. RESULTS: The combined treatment of HA and NP resulted in a remarkably improved control of green mould and natural decay in Chinese bayberries compared with treatment of HA or NP alone. The in vitro growth of P. citrinum was significantly inhibited by individual HA or NP. In addition, HA in combination with NP showed interactive effect on inhibiting fruit respiration, ethylene production and membrane lipid peroxidation, and maintained higher fruit firmness and DPPH radical scavenging activity. CONCLUSION: These results suggest that a combination of HA treatment and NP may be a useful technique to reduce fruit decay and maintain quality in Chinese bayberries during postharvest storage and retail conditions. Copyright © 2010 Society of Chemical Industry     

采前涂膜处理对宣化牛奶葡萄贮藏生理及品质的影响

研究采前涂膜处理对宣化牛奶葡萄的采后贮藏生理及保鲜效果。经采前挂蔓涂膜处理的牛奶葡萄在0℃条件贮藏100d,贮藏期间测定了果实的呼吸强度、乙烯释放量、总酚含量、叶绿素含量、可滴定酸含量等生理和品质指标。结果表明：采前涂膜处理可以显著抑制宣化牛奶葡萄果实在贮藏过程中的呼吸强度,处理组果实的呼吸强度比对照低42.5%～53.8%、并在一定程度上减少果实的乙烯释放量;采前涂膜能有效控制葡萄腐烂,贮藏结束时(100d)对照组腐烂率达75.3%,而涂膜处理组的腐烂率只有5.2%。同时可以减少褐变,贮藏75d时处理组的褐变指数比对照低81.6%。保持果实鲜绿色,处理组果实果皮叶绿素含量在50d和75d时为0.04mg/g和0.33mg/g,而对照组则为0.02mg/g和0.015mg/g;也可以保持葡萄果实可溶性固形物、可滴定酸含量,保持感官品质,延长保鲜期约50d。     

The effects of composite coatings containing chitosan and Mentha (piperita L. or x villosa Huds) essential oil on postharvest mold occurrence and quality of table grape cv. Isabella

The effects of coatings containing shrimp chitosan and the essential oil from Mentha piperita L. (MPEO) or M. villosa Huds (MVEO) to control common mold infections in table grape cv. Isabella (Vitis labrusca L.) that were caused by Aspergillus niger, Botrytis cinerea, Penicillium expansum and Rhizopus stolonifer were evaluated. The effects of the coatings on physicochemical and sensory characteristics of the grapes were also assessed. The coatings containing chitosan (4, 8 mg/mL) and MPEO or MVEO (1.25, 2.5, 5 μL/mL) delayed the mold growth and reduced the incidence of infections caused by all test fungi in grapes during storage at room and low temperatures. The coatings (chitosan 4 mg/mL; MPEO or MVEO 1.25, 2.5 μL/mL) did not negatively affect the physicochemical and sensory characteristics of grapes. From these results, coatings containing chitosan and MPEO or MVEO are potential postharvest treatments to control common mold infections in table grape cv. Isabella.Industrial relevanceIn table grapes, the reduction of losses resulting from fungal rot is a major goal of postharvest technology, which seeks to use effective methods to control the contamination and the growth of phytopathogenic fungi. However, the negative consumer perception of synthetic fungicides used for many years to solve the problem of mold infections in table grapes and the development of fungicide-resistant strains have impelled researchers to study the efficacy of natural compounds against postharvest pathogenic fungi. In this context, edible coatings composed of chitosan and essential oils have been considered as an environmentally friendly and added-value technology to control fungal postharvest decay in table grapes because of their biodegradability and lack of phytotoxicity. In this study, the authors evaluated composite coatings that contained shrimp chitosan and reduced amounts of Mentha (piperita L. or x vilosa Huds) essential oil as postharvest treatment to control the occurrence of mold infections caused by fungi in table grape cv. Isabella (Vitis labrusca L.), and their effects on the quality attributes of this fruit during storage. The tested composite coatings are presented as possible alternative technologies to control fungal infections and related post-harvest losses in table grape cv. Isabella.     

Effect of 1‐methylcyclopropene on anthracnose rot caused by Colletotrichum acutatum and disease resistance in loquat fruit

BACKGOUND: Loquat (Eriobotrya japonica Lindl.) fruit are susceptible to fungal decay during postharvest storage at ambient temperature. The control of postharvest diseases of loquat is based primarily on the use of synthetic fungicides. To look for an alternative method for disease control, we investigated the effect of 1‐methylcyclopropene (1‐MCP) on controlling anthracnose rot caused by Colletotrichum acutatum of loquat fruit. RESULTS: 1‐MCP treatment significantly reduced decay incidence of loquat fruit. The treatment markedly inhibited accumulation of superoxide radicals and hydrogen peroxide and maintained higher superoxide dismutase, catalase and ascorbate peroxidase. Meanwhile, 1‐MCP treatment induced higher activities of two defense‐related enzymes—chitinase and β‐1,3‐glucanase—during 6 days' storage. Furthermore, the treatment inhibited increases in fruit firmness and internal browning index, and maintained higher extractable juice rate, total soluble solids and titratable acidity contents, thereby delaying the development of senescence. CONCLUSION: 1‐MCP was effective in reducing decay and might have enhanced the disease resistance in loquat fruit by increasing chitinase and β‐1,3‐glucanase, and maintained natural resistance by delaying senescence development. Copyright © 2010 Society of Chemical Industry     

邻苯基苯酚结合果蜡涂膜处理对采后贡柑贮藏品质的影响

为延长贡柑贮藏期,探究邻苯基苯酚结合果蜡涂膜处理对采后贡柑果实的保鲜效果,本研究以采后德庆贡柑果实为实验材料,研究了0.2％浓度邻苯基苯酚结合果蜡涂膜处理对采后贡柑在常温贮藏期间果实品质的影响,观察和测定了贮藏期间果实的腐烂率、硬度、可滴定酸(TA)、可溶性固形物(TSS)、维生素C(VC)、细胞膜的通透性、丙二醛(M...     

奉节脐橙果皮褐变的调控技术研究

以奉园72-1 脐橙为材料,研究了田间喷钙、套袋处理、延迟采收、打蜡等对果实常温贮藏期间果皮褐变率和果实品质的影响。结果表明,田间喷钙结合套袋处理可显著降低果实贮藏期间的果皮褐变率(p<0.01),钙处理还显著提高了果面的红色,改善果实商品性;而延迟采收和打蜡均极显著地增加果皮褐变率(p<0.01);说明果树缺钙、涂蜡和不适采收期都是引发脐橙果皮褐变的重要因素。     

Efficacy of preharvest and postharvest Candida sake biocontrol treatments to prevent blue mould on apples during cold storage

This study compared the biocontrol efficiency of preharvest and postharvest applied yeast cells of Candida sake to apples (cv. Golden Delicious) wounded before and after harvest and inoculated with Penicillium expansum prior to cold storage conditions in two seasons, 1994/95 and 1995/96. The establishment of populations of C. sake during this period also was determined. In both years, postharvest treatment with the antagonist resulted in significant (P<0.05) and effective control of Penicillium rot whether pre- or postharvest wounds were made. Maximum disease control achieved, in terms of incidence and severity, was greater than 80% reduction in lesion diameter and 50% reduction in the incidence of lesions. However, preharvest application of the antagonistic yeast at a concentration of 3·10⁶ CFU/ml was less effective against Penicillium rot than postharvest treatment. No advantages in biocontrol were observed when apples were treated with the yeast antagonist both pre- and postharvest. Candida sake population levels during cold storage of apples receiving only preharvest application of the antagonist decreased prior and more rapidly than levels in apples receiving a postharvest application. High populations of C. sake were present in postharvest-treated apples, even after 90 days in cold storage.     

采前喷钙对贮藏期欧李果实腐烂率及糖酸含量的影响

研究采前一周喷施不同质量分数Ca(NO3)2溶液（1.0%、1.5%、2.0%）对贮藏期‘京欧1号’品种欧李果实腐烂率、糖酸含量及糖酸比值动态变化的影响。结果表明,采前喷钙降低了贮藏期欧李果实的腐烂率,增加了不同种类糖酸含量,特别是在贮藏45 d时,喷施1.5% Ca(NO3)2处理果实腐烂率为8.4%,并且果实中的蔗糖、葡萄糖、山梨糖醇、苹果酸、琥珀酸、酒石酸、柠檬酸和草酸含量以及糖酸比显著高于对照及其他喷钙处理。采前喷钙处理利于欧李果实的贮藏,以采前一周喷施1.5% Ca(NO3)2处理效果最好。     

采前喷施拮抗菌罗伦隐球酵母对草莓保鲜效果的影响

研究采前喷施拮抗菌罗伦隐球酵母(Cryptococcus laurentii)对常温贮藏(20℃,4d)后的\     

龙眼果实品质劣变和贮藏保鲜技术研究进展

龙眼是中国南方重要的亚热带特色水果,其果实成熟于高温季节,采后极易发生果皮褐变、果肉自溶及病原菌侵染所致果实腐烂等品质劣变,是限制龙眼果实采后保鲜期的主要因素。本文就龙眼果实采后品质劣变(果皮褐变、果肉自溶、病原菌侵染导致果实变质腐烂),热处理、紫外线-强效应波长(UV-B)辐照处理、二氧化氯处理、壳聚糖处理、低温贮藏、气调冷藏、微生物生物保鲜等龙眼果实采后处理及保鲜技术的国内外研究进展进行综述,旨在为延缓采后龙眼果实品质劣变、延长龙眼果实保鲜期提供技术参考。     

Shelf life extension of fresh fruit and vegetables by chitosan treatment

Among alternatives that are currently under investigation to replace the use of synthetic fungicides to control postharvest diseases in fresh produce and to extend their shelf life, chitosan application has shown promising disease control, at both preharvest and postharvest stages. Chitosan shows a dual mode of action, on the pathogen and on the plant, as it reduces the growth of decay-causing fungi and foodborne pathogens and induces resistance responses in the host tissues. Chitosan coating forms a semipermeable film on the surface of fruit and vegetables, thereby delaying the rate of respiration, decreasing weight loss, maintaining the overall quality, and prolonging the shelf life. Moreover, the coating can provide a substrate for incorporation of other functional food additives, such as minerals, vitamins, or other drugs or nutraceutical compounds that can be used to enhance the beneficial properties of fresh commodities, or in some cases the antimicrobial activity of chitosan. Chitosan coating has been approved as GRAS substance by USFDA, and its application is safe for the consumer and the environment. This review summarizes the most relevant and recent knowledge in the application of chitosan in postharvest disease control and maintenance of overall fruit and vegetable quality during postharvest storage.     

Effects of Pre- and Postharvest Chitosan Treatments to Control Storage Grey Mold of Table Grapes

ABSTRACT: The effectiveness of pre- and postharvest treatments with chitosan (0.1, 0.5, and 1.0%) to control Botrytis cinerea on table grapes was investigated. In postharvest treatments, small bunches dipped in chitosan solutions and inoculated with the pathogen showed a reduction of incidence, severity, and nesting of grey mold, in comparison with the control. Single berries artificially wounded, treated with the polymer, and inoculated with B. cinerea showed a reduced percentage of infected berries and lesion dia. Higher chitosan concentrations demonstrated greater decay reduction. All preharvest treatments significantly reduced the incidence of grey mold, as compared to the control. Table grapes treated with 1.0% chitosan showed a significant increase of phenylalanine ammonia-lyase (PAL) activity. Consequently, besides a direct activity against B. cinerea , chitosan produces other effects contributing to reduce decay.     

Control of Postharvest Blue Mold of Nanfeng Mandarin by Application of Strain YS‐1 Paenibacillus brasilensis

In order to study its commercial value, antagonistic spectrum and storage application of YS‐1 Paenibacillus brasilensis were investigated in this paper. YS‐1 P. brasilensis showed obvious antifungal activity to 5 different fruit pathogens, which was of broad antagonistic spectrum. Effect and application of YS‐1 P. brasilensis fermentation liquid on Nanfeng mandarin at different storage temperatures were also investigated with the puncture inoculation method. Results showed that lesion diameter and disease incidence at 25 °C were higher than those at 5 °C after end of the storage, and there was significant difference between them. P. brasilensis fermentation liquid was effective for control of Penicillium italicum on Nanfeng mandarin stored at 5 °C for 25 d or 25 °C for 20 d. Preharvest treatment combined with postharvest treatment significantly reduced the decay rate of Nanfeng mandarin by 5.8% more than the control, particularly in the 1st 2 mo of storage. Fruits treated with P. brasilensis fermentation liquid in preharvest and postharvest period tended to have higher total sugar content, titratable acidity, ascorbic acid (AsA) content, and soluble solids content than those in the control group, and there was significant difference between the 2 groups. A delay was observed in the drop in AsA content. In this article, strain YS‐1 is reported for the 1st time as a biocontrol agent against blue mold of Nanfeng mandarin. The research will provide an application reference for preservation of citrus.