石榴皮原花青素醇提条件的响应曲面优化及其抗氧化活性

在单因素实验的基础上,以提取时间、提取温度、料液比为自变量,原花青素得率为响应值,运用Box-Be-hnhen试验设计,采用响应面分析法对石榴皮原花青素的醇提条件进行优化。结果表明,最佳提取条件为:时间64.905 min,温度58.623℃,料液比1∶20.086。在此条件下,原花青素的得率达1.62%;含原花青素的醇提取物具有很强的还原力和较好螯合金属离子的能力。     

响应面法优化百香果中原花青素提取工艺研究

采用乙醇提取百香果皮中的原花青素,探讨反应时间、反应温度、固液比及乙醇体积分数对原花青素提取率的影响。结果表明,最佳提取工艺为:反应温度40℃,反应时间70 min,固液比1∶15 g/mL,乙醇体积分数55%,原花青素提取率1.82%,同时,于最佳工艺条件下探究了百香果籽中原花青素含量,结果表明,百香果籽中未含有原花青素成份。     

响应面法优化混合溶剂提取原花青素的研究

通过微波辅助混合溶剂提取技术结合响应面法优化原花青素提取条件,以期建立更高产率的提取方法。在单因素设计基础之上,选取液料比、微波功率、萃取时间、萃取温度4个主要因素对原花青素提取率的影响,建立多元回归拟合分析,得出原花青素提取最佳工艺条件为:液料比1:10,萃取温度61℃,微波功率625 W,萃取时间39 min,此条件下原花青素提取率1.78%,为预测值的89.45%。     

葡萄籽中原花青素提取工艺研究

通过浸提的方法从葡萄籽中提取原花青素,考察了不同溶剂、浸提时间、浸提温度、乙醇体积分数和料液比等单因素对浸提效果的影响,确定了最佳的单因素水平。并通过正交实验,得出了原花青素提取的最佳工艺条件:乙醇的体积分数为70%,提取温度为50℃,料液比为1∶7(g/mL),提取时间为30min,提取次数为3次。     

超声辅助提取落叶松树皮原花青素的工艺研究

对落叶松树皮中原花青素的超声波辅助提取工艺进行了研究,考察了提取温度、料液比、超声时间、溶剂浓度等4个因素对原花青素提取的影响。在单因素试验和正交试验的基础上,优选出超声波辅助提取落叶松树皮原花青素的最佳工艺条件为:以60%的乙醇溶液为溶剂,在50℃、料液比1∶25(g/mL)的条件下,用200 W的超声功率作用辅助提取60 min,原花青素的提取率可以达到1.0512%。     

原花青素提取、分离纯化方法研究进展

原花青素是一种具有多种重要生理活性的多酚类化合物。近年来,随着原花青素生理、药理活性被初步揭示,原花青素分离鉴定的研究正成为一个新的研究重点。参考了近年来国内外关于原花青素的大量研究,综述了原花青素提取、分离纯化方法的研究进展。     

微波辅助法提取紫甘薯中的原花青素及含量的测定

研究了以紫甘薯为原料,利用微波辅助法提取原花青素时提取剂浓度、料液比、微波处理时间等因素对提取率的影响。结果表明,在此最佳实验条件下,即以盐酸的乙醇溶液为提取液,在乙醇的体积分数为50％,料液比（g：mL）为1．70,微波处理70s,所得的原花青素浸提量为0．96mg／g。     

响应曲面法优化蜂胶中的活性黄酮提取

探讨了超声波辅助技术提取蜂胶中的活性黄酮类化合物的工艺。实验以芦丁为标样,在单因素实验基础上,采用Box-Behnken实验设计方法,以提取温度、固液比、提取时间以及乙醇浓度为自变量,黄酮提取率为响应值,研究各自变量及其交互作用对黄酮提取率的影响。利用Design expert软件,建立了提取率与超声波处理各因素的二次多项式数学模型,获得了蜂胶中黄酮的最佳提取条件为:提取温度51.32℃,固液比1∶10,提取时间12.88 min,乙醇浓度88.86%,最佳黄酮提取率为52.39%。经过实验验证,黄酮提取率可达51.98%。     

洋葱花青素提取工艺优化及其抗氧化活性研究

食品废弃物中有效成分再利用能提高资源利用率,其提取工艺成为近年来研究的热点。实验以紫洋葱皮为研究对象,利用单因素和Box-Behnken响应面优化法探索了其花青素提取的最佳工艺条件。实验利用水杨酸法、DPPH法和ABTS法进行了其抗氧化活性测定。结果表明,紫洋葱皮中花青素的最佳提取工艺：乙醇浓度为53%,料液比为1∶6(g∶mL),提取时间为73 min,提取温度为61℃,pH值为2。在此条件下,花青素的提取率为18.26%±0.04%。体外花青素抗氧化活性实验表明：花青素质量浓度为2.5 mg/L时,ABTS自由基清除率为79.66%;花青素质量浓度为1.0 mg/L时,·OH自由基清除率为70.65%,DPPH自由基清除率为77.97%。通过响应面法优化得到的该紫洋葱皮花青素提取工艺高效可靠,与阳性对照相比提取得到的花青素抗氧化活性较高。     

响应面法优化大豆荚壳异黄酮超声-回流萃取工艺

利用超声萃取和回流萃取方法进行单因素实验,选取提取温度、超声时间和乙醇体积分数为影响因子,应用响应面法(RSM)优化大豆荚壳异黄酮的提取条件。结果表明,大豆荚壳异黄酮优化浸提条件为:提取温度83℃、乙醇体积分数为84%、超声时间30min、大豆荚壳颗粒150μm、液料比25:1、回流萃取时间90min、超声功率108W,大豆荚壳总异黄酮的得率为3.5mg/g。响应面法大豆荚壳总异黄酮的提取条件优化合理可行,为提高大豆荚壳总异黄酮的提取率提供了依据。     

Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology

Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extraction conditions associated with anthocyanin yield, including extraction solvent, liquid-to-solid rate, temperature and extraction time, are discussed. The optimal conditions obtained by RSM for UAE from mulberry include 63.8% methanol contains 1% (v/v) trifluoroacetic acid (TFA), 43.2 °C temperature, 23.8 (v/w) liquid-to-solid ratio, and 40 min time for the maximum yield (64.70 ± 0.45 mg/g). The results indicated that the UAE can be an effective method for the extraction of some active components from plant materials.     

响应面法优化苦参碱粗品的结晶纯化过程

通过Box-Behnken试验设计,系统地考察了料液比、温度及结晶时间3个因素对苦参碱结晶收率的影响,并运用响应面法对建立的二次回归模型进行了综合分析。结果表明,当料液比为1∶28.78、温度为59.19℃、时间为30min时,苦参碱的结晶收率最大,其值为97.16%。     

Optimization of microwave-assisted extraction of bioactive compounds from pistachio (Pistacia vera L.) hull

ABSTRACT

Phenolic compounds were extracted from pistachio hull using microwave-assisted solvent extraction (MASE). The effects of four parameters, microwave power, extraction time, solvent to sample ratio, and ethanol concentration were evaluated. The extraction conditions were optimized by response surface methodology to enhance the total phenolic content (TPC). Optimal conditions were found as 140 W microwave power, 4.5 min extraction time, 19:1 (v/w) solvent to sample ratio, and 56% ethanol concentration to get maximum TPC (62.24 mg GAE/g dry hull). Also, MASE was compared with conventional solvent extraction (CSE) and MASE gave higher TPC, yield, and antioxidant activity.     

响应面分析法优化生姜挥发油提取工艺

在单因素实验的基础上,采用中心组合实验设计及响应面分析法优化了生姜中挥发油的水蒸汽蒸馏提取工艺。结果表明,提取时间(p<0.01),料液比(p<0.05)对挥发油提取得率具有显著影响。最佳工艺条件为:生姜干燥后粉碎过60目筛,加水15.6倍,水蒸汽蒸馏4.78 h。在此条件下,挥发油得率为2.00%,实验结果与模型预测值相符。     

响应面法优化螺旋藻中叶绿素的超声提取工艺

Chlorophylls were extracted by using ultrasonic from Spirulina platensis. Single factor examination and response surface analysis experiments were adopted to investigate the effects of extraction time, extraction solvent, solvent concentration, ratio of liquid to solid and extraction grade. The results showed that the optimal process parameters for this method were: extraction time of 56.5 min, ethanol concentration of 48.3% (vol) of ethanol/acetone solvent, and ratio of liquid to solid of 7. 9 ml·g^-1. The optimized chlorophylls extraction yield was 1.28%. The comparison experimental results indicated that the yield of chlorophylls by ultrasonic extraction was higher than that obtained from conventional solvent extraction.     

响应面法优化酶催化酯交换反应研究

为了利用植物油生产可再生的绿色能源——生物柴油,文章利用Novo435固定化脂肪酶,在无有机溶剂存在下催化菜籽油与甲醇酯交换合成生物柴油。利用响应面实验设计和分析方法对菜籽油的酯交换反应条件进行优化,得到了最佳工艺条件:醇油摩尔比1.5∶1,反应温度52℃,搅拌转速200 r/min,脂肪酶与油脂的质量比为10%,反应时间10 h,在此工艺条件下油脂的酯交换率达到48%(理论为50%)。理论甲醇量分3批加入,反应36 h后菜籽油的总酯交换率达到95%(理论酯交换率为100%)。每批试验后利用有机溶剂对脂肪酶进行清洗,然后继续反应,连续使用10个批次,油脂的酯交换率基本未变。     

Preparation of activated carbon from Jatropha hull with microwave heating: Optimization using response surface methodology

Preparation of activated carbon has been attempted using steam as the activating agent by microwave heating from Jatropha hull. The response surface methodology (RSM) technique is utilized to optimize the process conditions. The influences of the three major parameters, activation temperature, activation time and steam flow rate on the properties of activated carbon are investigated using analysis of variance (ANOVA), to identify the significant parameters. The optimum conditions for the preparation of activated carbon has been identified to be an activation temperature of 900 °C, activation time of 19 min and steam flow rate of 5 g/min. The optimum conditions resulted in an activated carbon with an iodine number of 988 mg/g and a yield of 16.56% respectively, while the BET surface area evaluated using nitrogen adsorption isotherm correspond to 1350 m²/g, with the pore volume of 1.07 cm³/g. The activated carbon is hetero porous with the micropore volume contributing to 40.8%.     

Optimization of enzymatic degumming process for rapeseed oil

An enzymatic process optimization and a largescale plant trial for rapeseed oil degumming were carried out by a novel microbial lipase. Response surface methodology was used to obtain the desired data in the process optimization. Enzyme dosage, temperature, and pH were important determining factors affecting oil degumming. The optimal set of variables was an enzyme dosage of 39.6 mg/kg, a temperature of 48.3°C, and a pH of 4.9. The phosphorus content could be reduced to 3.1 mg/kg at the optimal levels of the tested factors. An enzymatic degumming plant trial was performed on a 400 tons/d oil production line. pH was found to play an important role in degumming performance. When the pH was 4.6–5.1, the corresponding phosphorus content of degummed rapeseed oil could be reduced to less than 10 mg/kg, which met the demands of the physical refining process.