The effect of dietary α-tocopherol supplementation and antioxidant spraying on colour stability and lipid oxidation of turkey meat

Vitamin E was supplemented in the diet (250 mg kg^?1) of turkeys or sprayed directly on turkey meat. Turkey breast muscles were cut into 1.5 cm slices, wrapped with oxygen-permeable film and stored at 2–4°C for 1 week. Colour coordinates (L*, a*, b*), pH and reflectance values between 630 and 580 nm were determined at various times post mortem, TBARS (thiobarbituric acid-reactive substances) values and vitamin E content were assessed immediately after slaughter and at 7 days post mortem for vitamin E-supplemented, sprayed and control groups. Vitamin E supplementation or antioxidant spraying resulted in a lower myoglobin oxidation (P < 0.05) and a higher a* value at day 2, but not thereafter. No differences in TBARS values and ultimate pH were detected. When peroxidation was induced experimentally by addition of an activated mixture, vitamin E supplemented or antioxidant-sprayed samples exhibited lower TBARS values than the controls. Vitamin E supplementation in the diet or antioxidant spraying of meat produced a temporary delay in the rate of discoloration of turkey meat.     

Inhibition of oxidative rancidity in frozen cooked fish flakes by tert-butylhydroquinone and rosemary extract

The antioxidant properties of tert-butylhydroquinone (0·5 g kg^?1 + 20 g kg^?1 ascorbic acid—TBHQ-AS) and an extract of rosemary (2·5 g kg^?1) alone and in combination were determined by their addition as solutions to cooked fish flakes stored at - 20°C. Oxidation was measured by following changes in free fatty acids, thiobarbituric acid number, fatty acid composition and sensory evaluation. The order of effectiveness in inhibiting oxidation was TBHQ-AS > combination > rosemary > untreated control ?70°C > untreated control -20°C. Sensory evaluation indicated that green aroma and flavor notes were associated with the rosemary extract, while fish oil notes were associated with untreated samples.     

Intensity of lipid oxidation and formation of cholesterol oxidation products during frozen storage of raw and cooked chicken

Raw and cooked chicken breasts were stored at ?18 °C for 3 months under aerobic and vacuum conditions, and the intensity of lipid oxidation and the formation of COP (cholesterol oxidation products) were studied. Raw samples showed low COP levels (4.60–7.40 µg g^?1 fat), TBARS (thiobarbituric acid reactive substances) levels (0.01–0.03 mg kg^?1) and peroxide values (not detected) under both aerobic and vacuum conditions. Cooked samples (grilled and roasted) showed TBARS levels of 0.36–0.99 mg kg^?1 in aerobic conditions and 0.21–0.70 mg kg^?1 in vacuum conditions, whilst peroxide levels reached 38–40 and 19–23 meq O₂ kg^?1 in samples stored under aerobic and vacuum conditions respectively. Total COP levels in grilled and roasted samples were 28.91 and 39.34 µg g^?1 fat in aerobic packaging and 4.90 and 20.24 µ g g^?1 fat in vacuum packaging respectively. Significant correlations were found between the lipid oxidation parameters and cholesterol oxidation indices. In general, TBARS were better correlated with total COP than with only 7‐ketocholesterol. Vacuum packaging was particularly efficient in slowing down the oxidation process during frozen storage of cooked samples. Copyright © 2004 Society of Chemical Industry     

Vitamin E levels,thiobarbituric acid test and sensory evaluation of breast muscles from broilers fed α‐tocopheryl acetate‐ and β‐carotene‐supplemented diets

The objective of this study was to investigate the effect of dietary α‐tocopheryl acetate and β‐carotene supplementation on lipid oxidation of breast meat from broilers fed lard as the fat source. Supplementation of broilers with 100 mg kg^?1 α‐tocopheryl acetate increased the vitamin E levels in raw breast samples significantly (p < 0.05), whereas the presence of 1.5 mg kg^?1 dietary β‐carotene tended to decrease vitamin E deposition. The presence of vitamin E delayed lipid oxidation significantly, but thiobarbituric acid values of samples from broilers fed the β‐carotene‐supplemented diet did not differ from those of control samples. Vitamin E reduced sensory meat rancidity, whilst vitamin E, β‐carotene and their combination modified meat texture. The results show the effectiveness of dietary α‐tocopheryl acetate supplementation in protecting broiler meat against lipid oxidation. Copyright © 2004 Society of Chemical Industry     

The effect of storage temperatures on vitamin C and phenolics content of artichoke (Cynara scolymus L.) heads

The effect of temperatures (0, 2, 5, 7 and 10°C) for 14 days of storage on antioxidant constituents such as vitamin C and phenolic compounds of artichoke c.v. ‘Blanca de Tudela’ was studied. Three groups of phenolics were identified and quantified as: chlorogenic acid; 1,5-dicaffeoylquinic acid (1,5 di-CQA)+3,5-dicaffeoylquinic acid (3,5 di-CQA); and 1,4-dicaffeoylquinic acid (1,4-di-CQA)+4,5-dicaffeoylquinic acid (4,5 di-CQA). The content of vitamin C was higher in the internal bracts compared with the external ones (144 and 193 mg kg⁻¹ fresh wt., respectively) and decreased after 14 days of storage under the assayed temperatures. Each individual group of phenolics was 10-fold higher in the internal bracts than in the external ones. At harvest, total phenolics (618 mg kg⁻¹), chlorogenic acid (143 mg kg⁻¹) and 1,4 di-CQA+4,5 di-CQA (207 mg kg⁻¹), increased in internal bracts after storage, particularly at 2, 5 and 7°C. On the other hand, 1,5-di-CQA+3,5-di-CQA decreased from 260 to 150 mg kg⁻¹ after storage. Consumption of the edible artichoke fraction could be an important source of natural antioxidants and provide significant nutritional value to the diet.     

Comparative addition of rosemary extract and additives on sensory and antioxidant properties of retail packaged beef

The antioxidant capacity of an organoleptically mild, water‐ and oil‐soluble rosemary extract (RE) was compared with tert‐butyl‐4‐hydroxyanisol (BHA)/tert‐butyl‐4‐hydroxytoluene (BHT) and vitamin E (through dietary supplementation). An optimum concentration of RE (0.1%) was established from sensory trials and used for subsequent analyses. Beef burgers containing added antioxidants were held under simulated retail conditions and monitored for lipid (TBARS) and colour (Hunter ‘a’) stability. RE was more effective in controlling lipid oxidation in aerobically stored samples compared with control or BHA/BHT samples. Similar findings were observed for burgers stored under modified atmosphere although RE was also more effective than vitamin E under these conditions. Addition of RE to vitamin E supplemented beef resulted in lowest TBARS values and suggests a synergistic effect between RE and vitamin E. RE did not improve meat redness compared with other treatments. Water and oil (O/W) soluble RE offers considerable potential as a natural antioxidant for food products.     

Influence of the in vivo addition of alpha-tocopheryl acetate with three lipid sources on the lipid oxidation and fatty acid composition of Beluga sturgeon, Huso huso, during frozen storage

This study was conducted to investigate the effect of dietary alpha-tocopheryl acetate (vitamin E) and oil sources on fish flesh quality characteristics of Huso huso during frozen storage. Practical-type diets containing 0 or 250 mg vitamin E kg⁻¹ with three lipid sources, fish oil (FO), soybean oil (SO) and canola oil (CO), were fed to H. huso for 120 days. Fillet samples were analysed fresh or after storage at −18 ± 1 °C for 12 months. Replacement of FO by SO and CO in diets for H. huso significantly altered the fatty acid (FA) profile, which also influenced the FA composition during frozen storage. Dietary vitamin E had a significant effect on muscle vitamin E content and lipid oxidation during storage (P > 0.05). Oxidation was reduced for fish fed vitamin E and results showed that dietary vitamin E supplementation can slow down the level of lipid oxidation in H. huso muscles during frozen storage.     

The preservation of the quality of the muscle in frozen Australian red claw crayfish (Cherax quadricarinatus) by pre‐storage anti‐oxidant dipping treatments†

Australian red claw crayfish tails were dipped in either 0.06% (w/w) anti‐oxidant solutions (tocopherols, propyl gallate, or rosemary extract) or in water and subsequently stored in a ?20 °C freezer. At the end of 0, 1, 3, and 6 months of storage, the raw muscle samples were analysed for lipid oxidation, the thermal stability of proteins, cooking yield and shear force. Red claw treated with anti‐oxidants showed a lower (P < 0.05) thiobarbituric acid‐reactive substances (TBARS) production than the non‐anti‐oxidant control (dipped in water). Enthalpy of denaturation (ΔH) for the myosin head and shear values for the tails decreased (P < 0.05) in all samples after 6 months of storage, while cooking yield was unaffected by storage. Overall, red claw muscle was quite stable and the oxidation, measured as TBARS values, was small (<0.3 mg kg^?1) but could be further stabilized by anti‐oxidant dipping treatments. However the anti‐oxidant dipping did not prevent texture softening in red claw muscle during frozen storage.     

Quality characteristics of refined,bleached and deodorized palm olein and banana chips after deep‐fat frying

The changes in quality characteristics of refined, bleached and deodorized (RBD) palm olein used for deep‐fat frying of banana chips (180°C for 3 min at 17 min intervals for 2 h day^?1, to give six fryings per day and 30 fryings over five consecutive days) were studied. Four frying systems were used: RBD palm olein without antioxidant (system 1); RBD palm olein with 0.2 g kg^?1 α‐tocopherol (system 2); RBD palm olein with 1 g kg^?1 oleoresin rosemary (system 3); and RBD palm olein with the combination of 0.1 g kg^?1 α‐tocopherol + 0.5 g kg^?1 oleoresin rosemary (system 4). Fried oil samples were analysed for fatty acid composition, peroxide value (PV), anisidine value (AnV), totox value, free fatty acids (FFA) value, iodine value, at 232 and 268 nm, colour and viscosity. The crispness and sensory evaluation of the banana chips fried in this study were also evaluated. The results showed that RBD palm olein with added antioxidant reduced PV, AnV, totox value, at 232 and 268 nm, FFA and viscosity. The order of effectiveness in inhibiting oil oxidation in RBD palm olein was: oleoresin rosemary > α tocopherol + oleoresin rosemary > α‐tocopherol > control. For the crispness, the force values of banana chips fried in all systems were comparable. Sensory evaluation of fried banana chips for each system showed that there was no significant (P > 0.05) difference in terms of colour, flavour, odour, texture and overall acceptability. Copyright © 2003 Society of Chemical Industry     

Effects of rosemary and sage tea extracts on the sensory,chemical and microbiological changes of vacuum‐packed and refrigerated sardine (Sardina pilchardus) fillets

The effect of the natural antioxidants and antimicrobials from ethanol extracts of rosemary and sage tea on sensory, chemical [Total volatile basic nitrogen (TVB‐N), thiobarbituric acid reactive substances (TBARs), peroxide value (PV), free fatty acid (FFA)] and microbiological (total viable count‐TVC and total coliform count) changes of vacuum‐packaged sardine (Sardina pilchardus) fillets stored at 3 ± 1 °C was investigated for 20 days. The fish fillets were divided into three groups: untreated group (control, C) and treated groups that were immersed in a 1 L of distilled water containing 10 g rosemary (R group) or sage tea (S group) extracts for 4 min. The shelf life of sardine fillets was found to be 13 days for control (C), 20 days for R and S groups according to sensory assessment results, whose corresponded microbiological assessment showed a shorter shelf life (5 days for C group, 9 for R and S groups). At the end of storage period, TBARs values were 0.98 mg malonaldehyde kg^?1 for C group, 0.66 malonaldehyde kg^?1 for R group and 1.44 mg malonaldehyde kg^?1 for S group. Microbiological results showed that natural compounds from rosemary and sage tea resulted in a lower bacterial growth in fish fillets during the storage period.     

Improving the Sensory and Oxidative Stability of Cooked and Chill‐Stored Lamb Using Dietary Rosemary Diterpenes

Two dietary rosemary extracts (DREs) containing diterpenes (carnosic acid and carnosol at 1:1 and 2:1 w:w) were tested in fattening lambs to stabilize the sensory quality of cooked and chill‐stored patties. A total of 63 lambs were fed freely for 80 ± 5 d with a basal diet supplemented or not with DRE. Minced leg meat from each lamb was used to make patty batches. The patties were cooked at 72 ºC for 2 min, aerobically packed, kept at 2 ºC for up to 4 d and then reheated. Sensory traits (color, odor, flavor, and texture), CIELab color, and lipid oxidation (assessed as TBARS) were determined. In a first experiment, the lamb diet was supplemented with 600 mg of 1:1‐DRE or 2:1‐DRE kg^?1 feed. The 1:1‐DRE diet delayed discoloration, flavor deterioration, and rancidity, while the 2:1‐DRE diet was ineffective in this respect. In a second experiment, 4 supplementation levels of 1:1‐DRE (0, 200, 400, and 600 mg kg^?1 feed) were compared. Flavor deterioration was delayed when the lamb diet was supplemented with at least 400 mg 1:1‐DRE kg^?1 feed. The effects of the diet on the odor, flavor, and color were corroborated by differences in TBARS and CIELab. The results obtained suggest that rosemary diterpenes and/or their active secondary compounds deposited in muscle can act as endogenous antioxidants in cooked lamb. The carnosol intake seems crucial in the antioxidant actions achieved through DRE. The use of rosemary antioxidants in animal feeding would allow meat‐based dishes to be preserved longer without adding preservatives.     

香辛料提取物对低温油炸肉丝软罐头的抗氧化效应研究

目的通过添加具有抗氧化活性和较高安全性的香辛料提取物,解决中式肉类菜肴的软罐头食品中变色、风味变差以及脂肪氧化酸败等问题。方法向低温油炸肉丝软罐头中添加不同添加量的迷迭香、肉桂和VE提取物以及2种人工合成抗氧化剂丁基羟基茴香醚(butyl hydroxy anisd,BHA)和没食子酸丙酯(propylgallate,PG),测定其对软罐头菜肴产品在贮藏过程中硫代巴比妥酸值(thiobarbituric acid-reacitive substances,TBARS值)、过氧化物值(peroxide value,POV值)和色差值的影响。结果添加的3种共9组天然抗氧化剂中,迷迭香提取物抑制初级氧化产物氢过氧化物和次级氧化产物丙二醛的效果最好(P0.05)。添加300 mg/kg迷迭香、90 mg/kg PG和120 mg/kg BHA的3组处理组在21 d冷藏期内,其POV值和TBARS值显著小于其他各组(P0.05),说明添加300 mg/kg迷迭香可以替代人工抗氧化剂。结论低温油炸肉丝软罐头中添加300 mg/kg迷迭香提取物,可以减缓脂肪氧化速度,进而延长产品的货架期。     

Fortification of low-nitrite canned pork with willow herb (Epilobium angustifolium L.)

Nitrite is a multifunctional additive used for meat products. However, cancerogenic N-nitrosoamines (NAs) can be formed from nitrite. For this reason, nitrite should be reduced or eliminated. This study aimed to (1) determine the phenolic content, composition, and in vitro antioxidant and antimicrobial activity of water lyophilized extracts of willow herb (WH); and (2) assess the effect of the extracts on lipid oxidation, colour, microbiological and chemical safety of canned pork with lower nitrite content during storage. For the preparation of extract, redistilled water at a temp. of 90°C (1:10) was used. The extracts were filtered and freeze-dried for 72 h (−80°C, 0.04 mbar). Five different variants of product were tested in the study: control (with 100 mg kg⁻¹ NaNO₂) and herbal samples (with 50, 100, 150, and 1000 mg kg⁻¹ WH). The samples were assessed for their colour, antioxidant capacity, lipid oxidation, pH, sensory characteristics, and the presence of N-nitrosamines and pathogenic bacteria. Oenothein B was found as the predominating compound in the analysed extract (HPLC) followed by querceti-3-O-rhamnoside and oenothein A, myricetin 3-O-glucoside. The addition of WH extract contributed to maintaining the typical, cured colour of canned meat as well as reducing the NaNO₂ content. Furthermore, fortification with 150 mg kg⁻¹ of Epilobium angustifolium L. extract allowed stabilising the antioxidant properties of canned meat during storage to a greater extent compared to 1000 mg kg⁻¹ of extract.     

The effects of rosemary extract combination with vacuum packing on the quality changes of Atlantic mackerel fish burgers

The effects of the use of antioxidant technology on the quality of mackerel burgers stored at 4 °C were investigated in terms of sensory, biochemical [thiobarbituric acid (TBA), total volatile basic nitrogen (TVB‐N), peroxide value (PV) and free fatty acids (FFA)] and microbiological analyses [total viable count (TVC)]. Fish meat was divided into three groups; the control (C) without rosemary extract, the second group with 0.4% rosemary extracts (Group A) and the third with 0.8% rosemary extracts (Group B). Results showed that the TVB‐N level did not exceed acceptability limit (30–35 mg 100 g^?1) for all groups. TBA value for the control significantly increased from 1.47 to 4.80 MA kg^?1 whereas it slightly increased in treatment groups as the storage time increased. Significantly higher PV and FFA (P < 0.05) were obtained from the control group. Rosemary extract, in combination with vacuum pack was effective in controlling the growth of bacteria and biochemical indices.     

Nutritional and sensory qualities of raw meat and cooked brine‐injected turkey breast as affected by dietary enrichment with docosahexaenoic acid (DHA) and vitamin E

BACKGROUND: This aim of this study was to evaluate the effects of feeding turkeys with docosahexaenoic acid (DHA) and vitamin E on the fatty acid profile, proteolytic enzyme activities and oxidative status of raw breast meat and cooked brine‐injected breast meat. Four treatments were investigated: T1, basal diet (control); T2, basal diet plus 15 g kg^?1 DHA; T3, basal diet plus 100 mg kg^?1 vitamin E; T4, basal diet plus 5.4 g kg^?1 DHA plus 100 mg kg^?1 vitamin E. A sensory analysis of cooked brine‐injected breasts was conducted in order to assess the sensory characteristics of these products and relate them to the expected nutritional benefits. RESULTS: Among the four treatments tested, no differences were observed in enzyme activities. No activities of cathepsin B, cathepsins B + L and catalase were detected in cooked brine‐injected breast meat. Glutathione peroxidase activity was reduced and superoxide dismutase activity was similar to that measured in raw meat. The diets supplemented with DHA increased eicosapentaenoic acid and DHA levels in comparison with the control in both raw and cooked products. The increase in n‐3 polyunsaturated fatty acids (PUFAs) led to a reduction in n‐6/n‐3 PUFA ratio to values between 1.01 ± 0.11 and 2.94 ± 0.47. In cooked brine‐injected breast, treatment with 15 g kg^?1 DHA (T2) induced a considerable fishy flavour, while treatment with 5.4 g kg^?1 DHA plus 100 mg kg^?1 vitamin E (T4) induced a slight fishy flavour. However, fishy odour in T4 did not differ significantly from that of the control. CONCLUSION: By feeding turkeys with 5.4 g kg^?1 pure DHA plus 100 mg kg^?1 vitamin E, the nutritional quality is improved through the introduction of a natural antioxidant and the reduction in n‐6/n‐3 PUFA ratio. With this treatment the sensory characteristics were similar to those of control samples, except for the fishy flavour, which could probably be masked by modifying the technological process. Copyright © 2008 Society of Chemical Industry     

Ability of α-tocopherol,taurine and rosemary,in combination with vitamin C,to increase the oxidative stability of beef steaks packaged in modified atmosphere

Fresh beef steaks were sprayed on the surface with vitamin C (500 ppm), taurine (50 mM), rosemary (1000 ppm) and vitamin E (100 ppm), the three latter in combination with 500 ppm of Vitamin C, packaged in modified atmosphere (70% O₂+20% CO₂+10% N₂) and stored at 1±1 °C for 29 days. Metmyoglobin formation, lipid oxidation (TBARS), instrumental colour (CIE a¹), psychrotrophic bacterial counts (PCA) and sensory discolouration and odour were determined. Results demonstrated that surface application of antioxidant combinations resulted in an effective delay of oxidative deterioration of fresh beef steaks. Shelf life was extended beyond that of control, according to evaluation of sensory attributes. Both combinations of vitamin C with either rosemary or taurine significantly (P<0.01) extended the shelf life of fresh beef steaks by about 10 days. Rosemary was the most effective in delaying oxidation processes. The combination of vitamins E and C was significantly (P<0.01) less effective than those combinations in delaying meat oxidation.     

The pre- and post-grinding application of rosemary and its effects on lipid oxidation and color during storage of ground beef

The timing of the application of rosemary extract was evaluated as one-way of minimizing myoglobin and lipid oxidation in ground beef. In experiment 1, rosemary extract was added to beef at four different stages namely trim, cube, coarse, and fine ground beef. The beef was evaluated for color and TBARS values during 144 h of storage (4 °C). Results showed that when rosemary was added to the pre-grinding treatments of trim and cube, ground beef had the highest a^∗ values (redness), oxymyoglobin content, and lowest TBARS values at 144 h. In experiment 2, the effect of rosemary extract was evaluated on the color quality of case ready ground beef inoculated with 10⁷ CFU/g Escherichia coli. Microbial counts, color, and TBARS values were measured during 144 h of simulated storage. The results showed that both the rosemary treated samples that were inoculated and uninoculated remained redder longer and had lower TBARS values than the untreated inoculated and uninoculated controls. There was no significant inhibition of E. coli by the rosemary extract.     

Effectiveness of organic acid,ozonated water and chlorine dippings on microbial reduction and storage quality of fresh‐cut iceberg lettuce

BACKGROUND: The comparative effects of organic (citric and lactic) acids, ozone and chlorine on the microbiological population and quality parameters of fresh-cut lettuce during storage were evaluated. RESULTS: Dipping of lettuce in 100 mg L⁻¹ chlorine solution reduced the numbers of mesophilic and psychrotrophic bacteria and Enterobacteriaceae by 1.7, 2.0 and 1.6 log₁₀ colony-forming units (CFU) g⁻¹ respectively. Treatment of lettuce with citric (5 g L⁻¹) and lactic (5 mL L⁻¹) acid solutions and ozonated water (4 mg L⁻¹) reduced the populations of mesophilic and psychrotrophic bacteria by 1.7 and 1.5 log₁₀ CFU g⁻¹ respectively. Organic acid dippings resulted in lower mesophilic and psychrotrophic counts than ozonated water and chlorine dippings during 12 days of storage. Lactic acid dipping effectively reduced (by 2.2 log₁₀ CFU g⁻¹) and maintained low populations of Enterobacteriaceae on lettuce for the first 6 days of storage. No significant (P > 0.05) changes were observed in the texture and moisture content of lettuce samples dipped in chlorine, organic acids and ozonated water during storage. Colour, β-carotene and vitamin C values of fresh-cut iceberg lettuce did not change significantly (P > 0.05) until day 8. CONCLUSION: Lactic and citric acid and ozonated water dippings could be alternative treatments to chlorine dipping to prolong the shelf life of fresh-cut iceberg lettuce. Copyright © 2007 Society of Chemical Industry     

Degree of oxidation of sonicated soybean oil using various sonication process parameters

This research aimed to examine the effect of high-intensity ultrasound (HIU) on the oxidation of soybean oil (SBO). Samples (5, 100, and 250 g) were sonicated at 25 °C using various sonication conditions: power level (settings 1, 5, and 9), tip size (12.7, 3.2, and 2 mm), and duration (5, 10, and 60 s). After sonication, the degree of oxidation of samples (sonicated and non-sonicated SBO) were analysed using peroxide value (PV), oil stability index (OSI), and volatile compounds. Sonication was applied to obtain a range of absolute power (0–123.5 W), power density (0–14.2 W cm⁻³), and power intensity (0–1,120 W cm⁻²) values. The highest power density and power intensity were obtained with the highest HIU setting with the 3.2 mm tip. The highest power was obtained when HIU was applied using a 12.7 mm tip and setting 9 in 250 g of sample. No significant difference (P > 0.05) was observed in PV (0.20 ± 0.01 mEq kg⁻¹) nor OSI (10.05 ± 0.04 h), among the various sonication conditions tested. In addition, no differences were observed in the volatile compounds obtained. These results show that HIU can be used under mild sonication conditions such as the ones needed to change the physical properties of lipids without causing oxidative deterioration.