Economic feasibility evaluation of microfiltration of milk prior to cheesemaking

A nonlinear programming optimization model was used to evaluate the net revenues and potential profit-ability of microfiltration (MF) prior to cheesemaking in the 3-year period 1998 to 2000, using monthly milk price and composition data. The model identifies the optimal mix of milk resources and determines if MF cheesemaking produces a higher net revenue than conventional cheesemaking that uses NDM and condensed milk for fortification. This study demonstrates the potential of this model to evaluate new technologies in cheese manufacture and improve decision making in the cheese industry. The use of MF produced higher net revenues in 30 out of the 36 mo for both Cheddar and low-moisture, part-skim mozzarella, leading to an appreciable increase in net revenue (vs. conventional cheesemaking) for both cheeses. The benefit from MF in net revenue was greater when the cream price was high. The use of 3X MF yielded the same net revenue as 2X MF. An estimate of manufacturing costs of MF vs. conventional cheesemaking was also made. To this end, the yields of products were calculated by the optimization model, while the production cost of each product was estimated from data of two economic engineering studies and a MF cheesemaking trial. The manufacturing cost of MF Cheddar was slightly higher than the manufacturing cost of conventional Cheddar. However, the benefit in net revenue from the use of MF was estimated to be higher than the difference in manufacturing costs. Moreover, some advantages in the new coproducts of MF Cheddar could outweigh its higher manufacturing cost. The relationships between prices and recoveries of coproducts required to render MF profitable were identified.     

Valuation of milk composition and genotype in cheddar cheese production using an optimization model of cheese and whey production

A mass balance optimization model was developed to determine the value of the κ-casein genotype and milk composition in Cheddar cheese and whey production. Inputs were milk, nonfat dry milk, cream, condensed skim milk, and starter and salt. The products produced were Cheddar cheese, fat-reduced whey, cream, whey cream, casein fines, demineralized whey, 34% dried whey protein, 80% dried whey protein, lactose powder, and cow feed. The costs and prices used were based on market data from March 2004 and affected the results. Inputs were separated into components consisting of whey protein, ash, casein, fat, water, and lactose and were then distributed to products through specific constraints and retention equations. A unique 2-step optimization procedure was developed to ensure that the final composition of fat-reduced whey was correct. The model was evaluated for milk compositions ranging from 1.62 to 3.59% casein, 0.41 to 1.14% whey protein, 1.89 to 5.97% fat, and 4.06 to 5.64% lactose. The κ casein genotype was represented by different retentions of milk components in Cheddar cheese and ranged from 0.715 to 0.7411 kg of casein in cheese/kg of casein in milk and from 0.7795 to 0.9210 kg of fat in cheese/kg of fat in milk. Milk composition had a greater effect on Cheddar cheese production and profit than did genotype. Cheese production was significantly different and ranged from 9,846 kg with a high-casein milk composition to 6,834 kg with a high-fat milk composition per 100,000 kg of milk. Profit (per 100,000 kg of milk) was significantly different, ranging from $70,586 for a high-fat milk composition to $16,490 for a low-fat milk composition. However, cheese production was not significantly different, and profit was significant only for the lowest profit ($40,602) with the κ-casein genotype. Results from this model analysis showed that the optimization model is useful for determining costs and prices for cheese plant inputs and products, and that it can be used to evaluate the economic value of milk components to optimize cheese plant profits.     

Survival of foot-and-mouth disease virus in cheese.

Persistence of foot-and-mouth disease virus during the manufacture of Cheddar, Mozzarella, Camembert cheese prepared from milk of cows experimentally infected with the virus was studied. Cheese samples were made on a laboratory scale with commercial lactic acid starter cultures and the microbial protease MARZYME as a coagulant. Milk was heated at different temperatures for different intervals before it was made into cheese. Food-and-mouth disease virus survived the acidic conditions of Cheddar and Camembert cheese processing but not that of Mozzarella. Foot-and-mouth disease virus survived processing but not curing for 30 days in Cheddar cheese preparaed from heated milk. However, the virus survived curing for 60 days but not for 120 days in cheese (pH 5) prepared from unheated milk. Foot-and-mouth disease virus survived in Camembert cheese (pH 5) for 21 days at 2 C but not for 35 days.     

Fortification of cheese with vitamin D(3) using dairy protein emulsions as delivery systems

Vitamin D is an essential vitamin that is synthesized when the body is exposed to sunlight or after the consumption of fortified foods and supplements. The purpose of this research was to increase the retention of vitamin D(3) in Cheddar cheese by incorporating it as part of an oil-in-water emulsion using a milk protein emulsifier to obtain a fortification level of 280 IU/serving. Four oil-in-water vitamin D emulsions were made using sodium caseinate, calcium caseinate, nonfat dry milk (NDM), or whey protein. These emulsions were used to fortify milk, and the retention of vitamin D(3) in cheese curd in a model cheesemaking system was calculated. A nonemulsified vitamin D(3) oil was used as a control to fortify milk. Significantly more vitamin D(3) was retained in the curd when using the emulsified vitamin D(3) than the nonemulsified vitamin D(3) oil (control). No significant differences were observed in the retention of vitamin D(3) when emulsions were formulated with different emulsifiers. Mean vitamin D(3) retention in the model system cheese curd was 96% when the emulsions were added to either whole or skim milk compared with using the nonemulsified oil, which gave mean retentions of only 71% and 64% when added to whole and skim milk, respectively. A similar improvement in retention was achieved when cheese was made from whole and reduced-fat milk using standard manufacturing procedures on a small scale. When sufficient vitamin D(3) was added to produce cheese containing a target level of approximately 280 IU per 28-g serving, retention was greater when the vitamin D(3) was emulsified with NDM than when using nonemulsified vitamin D(3) oil. Only 58±3% of the nonemulsified vitamin D(3) oil was retained in full-fat Cheddar cheese, whereas 78±8% and 74±1% were retained when using the vitamin D(3) emulsion in full-fat and reduced-fat Cheddar cheese, respectively.     

Effect of heat treatments on the meltability of cheeses

The effect of heat treatments on the meltability of cheese was investigated. Cheddar cheeses of different composition and low-moisture, part-skim Mozzarella cheese were studied at 1, 3, 6, and 12 wk of aging. Cheese samples were heated to 60 degrees C and held for 0, 10, and 20 min before allowing the melted cheese to flow. Mean meltabilities, over all ages, of both Cheddar and Mozzarella cheeses decreased significantly as holding time increased. Meltability of young cheese was scarcely affected by the holding time, in sharp contrast to that of the old cheese where increasing the holding time greatly reduced meltability.     

EFFECTS of STERILIZING DOSES of GAMMA IRRADIATION ON LEVELS of VITAMIN A, AMINO ACIDS, and FATTY ACIDS IN SELECTED DAIRY PRODUCTS

Frozen dairy products intended for low microbial diets of immunosuppressed patients were sterilized by exposure to 40 kGy (4 Mrad) of cobalt-60 irradiation. Levels of vitamin A, amino acids, and selected fatty acids were measured in yogurt bars, ice cream, Cheddar cheese, mozzarella cheese, and nonfat dry milk before and after irradiation. Addition of antioxidants to the frozen dairy desserts prior to irradiation at - 78C was not effective in preventing degradation of vitamin A. There were significant decreases of approximately 90% in levels of vitamin A in Cheddar cheese and mozzarella cheese irradiated at -78C and 0–5C compared to the corresponding nonirradiated products. Levels of vitamin A in nonfat dry milk irradiated at three different temperatures (22C, 0–5C, or - 78C) were variable compared to levels in the nonirradiated product. Levels of palmitic, stearic, oleic, linoleic, and linolenic acids were retained in all irradiated dairy products analyzed. Irradiation at the specified temperatures did not affect levels of individual amino acids in nonfat dry milk or mozzarella cheese.     

Development and application of a processing model for the Irish dairy industry

A processing-sector model was developed that simulates (i) milk collection, (ii) standardization, and (iii) product manufacture. The model estimates the product yield, net milk value, and component values of milk based on milk quantity, composition, product portfolio, and product values. Product specifications of cheese, butter, skim and whole milk powders, liquid milk, and casein are met through milk separation followed by reconstitution in appropriate proportions. Excess cream or skim milk are used in other product manufacture. Volume-related costs, including milk collection, standardization, and processing costs, and product-related costs, including processing costs per tonne, packaging, storage, distribution, and marketing, are quantified. Operating costs, incurred irrespective of milk received and processing activities, are included in the model on a fixed-rate basis. The net milk value is estimated as sale value less total costs. The component values of fat and protein were estimated from net milk value using the marginal rate of technical substitution. Two product portfolio scenarios were examined: scenario 1 was representative of the Irish product mix in 2000, in which 27, 39, 13, and 21% of the milk pool was processed into cheese (€3,291.33/t), butter (€2,766.33/t), whole milk powder (€2,453.33/t), and skim milk powder (€2,017.00/t), respectively, and scenario 2 was representative of the 2008 product mix, in which 43, 30, 14, and 13% was processed into cheese, butter, whole milk powder, and skim milk powder, respectively, and sold at the same market prices. Within both scenarios 3 milk compositions were considered, which were representative of (i) typical Irish Holstein-Friesian, (ii) Jersey, and (iii) the New Zealand strain of Holstein-Friesian, each of which had differing milk constituents. The effect each milk composition had on product yield, processing costs, total revenue, component values of milk, and the net value of milk was examined. The value per liter of milk in scenario 1 was 24.8, 30.8, and 27.4 cents for Irish Holstein-Friesian, Jersey, and New Zealand strain of Holstein-Friesian milk, respectively. In scenario 2 the value per liter of milk was 26.1, 32.6, and 28.9 cents for Irish Holstein-Friesian, Jersey, and New Zealand strain of Holstein-Friesian milk, respectively.     

Impact of low concentration factor microfiltration on the composition and aging of Cheddar cheese

The effect of microfiltration (MF) on proteolysis, hardness, and flavor of Cheddar cheese during 6 mo of aging was determined. Raw skim milk was microfiltered two-fold in two cheese making trials. In trial 1, four vats of cheese were made in 1 d using unconcentrated milk (1X), 1.26X, 1.51X, and 1.82X concentration factors (CF). Casein-(CN)-to-fat ratio was constant among treatments. Proteolysis during cheese aging decreased with increasing CF due to either limitation of substrate availability for chymosin due to low moisture in the nonfat substance (MNFS), inhibition of chymosin activity by high molecular weight milk serum proteins, such as alpha2-macroglobulin, retained in the cheese or low residual chymosin in the cheese. Hardness of fresh cheese increased, and cheese flavor intensity decreased with increasing CF. In trial 2, the 1X and 1.8X CF were compared directly. Changes made in the cheese making procedure for the 1.8X CF (more chymosin and less cooking) increased the MNFS and made proteolysis during aging more comparable for the 1X and 1.8X cheeses. The significant difference in cheese hardness due to CF in trial 1 was eliminated in trial 2. In a triangle test, panelists could not differentiate between the 1X and 1.8X cheeses. Therefore, increasing chymosin and making the composition of the two cheeses more similar allowed production of aged Cheddar cheese from milk concentrated up to 1.8X by MF that was not perceived as different from aged Cheddar cheese produced without MF.     

发酵剂、氯化钙及凝乳酶添加量对白牦牛乳Mozzarella干酪质构的影响

以白牦牛乳为原料制作Mozzarella干酪,在单因素试验基础上采用Box-Behnken响应面法对发酵剂、CaCl_2、凝乳酶添加量进行优化,利用质构综合评分筛选最佳的工艺参数。结果表明,白牦牛乳Mozzarella干酪的最佳工艺参数为:发酵剂添加量0.24 g/L、CaCl_2添加量0.23 g/L、凝乳酶添加量0.04 g/L,此时白牦牛乳Mozzarella干酪质构综合评分为11.095,出品率为20.53%,感官评分为93.67。此工艺条件下制作的白牦牛乳Mozzarella干酪香味浓郁,质地均匀,软硬适度。     

Cheddar Cheese from Ultrafiltered Whole Milk Retentates in Industrial Cheese Making

Transporting whole milk retentates of ultrafiltration to a distant large industrial Cheddar cheese making site resulted in 16 lots of Cheddar cheese from vats containing 2,546 to 16,360 kg of cheese milk. Whole milk retentates concentrated by ultrafiltration to 4.5:1 were added to cheese milks to give mixtures concentrated 1.2:1 and 1.3:1 with approximately 20 and 30% more protein and fat, respectively, than in unsupplemented control whole milks or unsupplemented commercial reference milks.Gross composition of Cheddar cheese made from commercial reference, control, and retentate-supplemented milk generally showed no major differences. Yield increased in cheese made from retentate-supplemented milk. Yield efficiency per kilogram total solids rose in retentate cheese over controls but not among commercial reference, control, and retentate lots based on per kilogram fat or total protein. Milk components were higher in wheys from retentate cheeses, but loss of components per kilogram cheese obtained generally showed lower values in whey from retentate cheese.General quality of retentate Cheddar cheese was equal to that of reference unsupplemented commercial cheese and higher than unsupplemented control Cheddar cheeses. It appears technically feasible to ultrafilter milk at one site, such as the farm, collecting station, or specialized center, and transport it to an industrial site for Cheddar cheese making.     

USE OF MILK CONCENTRATED BY ULTRAFILTRATION FOR MAKING HARD CHEESE,SOFT CHEESE AND YOGHURT*

Whole milk was concentrated by a factor of two by ultrafiltration. It was used directly for making Cheddar and Cheshire cheese, an unripened soft cheese of the Coulommier type, and yoghurt. The yields of hard cheese from the concentrated milk were the same as those from normal whole milk. The cheeses were acceptable though the flavour was milder than that of good quality Cheddar and Cheshire cheese. Medium fat soft cheeses were made from the concentrated milk. The yield of cheese was 41 per cent greater than that made from normal whole milk and the making time was half that of the normal process. The cheeses were consumed fresh or stored in deep freeze. For making yoghurt, the usual reinforcement with skim milk powder was not necessary as the concentrated milk had a high total solids content, nor was it necessary to homogenize the mix. The yoghurt contained 21 per cent total solids and was a very acceptable product.     

Application of exopolysaccharide-producing cultures in reduced-fat Cheddar cheese: composition and proteolysis

Proteolysis during ripening of reduced fat Cheddar cheeses made with different exopolysaccharide (EPS)-producing and nonproducing cultures was studied. A ropy strain of Lactococcus lactis ssp. cremoris (JFR1) and capsule-forming nonropy and moderately ropy strains of Streptococcus thermophilus were used in making reduced-fat Cheddar cheese. Commercial Cheddar starter was used in making full-fat cheese. Results showed that the actual yield of cheese made with JFR1 was higher than that of all other reduced-fat cheeses. Cheese made with JFR1 contained higher moisture, moisture in the nonfat substance, and residual coagulant activity than all other reduced-fat cheeses. Proteolysis, as determined by PAGE and the level of water-soluble nitrogen, was also higher in cheese made with JFR1 than in all other cheeses. The HPLC analysis showed a significant increase in hydrophobic peptides (causing bitterness) during storage of cheese made with JFR1. Cheese made with the capsule-forming nonropy adjunct of S. thermophilus, which contained lower moisture and moisture in the nonfat substance levels and lower chymosin activity than did cheese made with JFR1, accumulated less hydrophobic peptides. In conclusion, some EPS-producing cultures produced reduced-fat Cheddar cheese with moisture in the nonfat substance similar to that in its full-fat counterpart without the need for modifying the standard cheese-making protocol. Such cultures might accumulate hydrophobic (bitter) peptides if they do not contain the system able to hydrolyze them. For making high quality reduced-fat Cheddar cheese, EPS-producing cultures should be used in conjunction with debittering strains.     

原辅料对再制马苏里拉干酪品质特性的影响

以天然马苏里拉干酪为目标,以干酪融化后特性(融化性、油脂析出率、拉伸性和褐变性)、感官、微观结构为指标,研究原辅料对再制马苏里拉干酪融化后品质特性的影响。结果表明:选择比例为1∶1(m/m,下同)的成熟度为3个月切达干酪和马苏里拉干酪,配合比例为1∶4色拉油和奶油进行再制马苏里拉干酪的制作,既保证了再制干酪所需的质构,同时也赋予产品特有的风味;添加量为1.3%的乳化盐和2.0%的柠檬酸能提供再制干酪所需的乳化性和合理的p H值,产品的加工特性最佳。     

Economic Value of Milk Components for Fluid Milk,Cheese, Butter,and Nonfat Dry Milk and Responses to Selection

The consequences of genetically and mechanically altering milk composition were investigated. A base price plus a component differential method of payment was shown to be equivalent to the sum of the components of milk yield (carrier, fat, and protein) times their relative economic value. Relative economic values of carrier, fat, and protein were derived for fluid milk. Linear programming models were developed to simulate the movement of milk through cheese and through butter and NDM manufacturing processes. These models were used to estimate the cost of moving milk through the respective plants, and these values were equated to the “breakeven” cost of carrier. New economic values for fat and protein were developed on these respective values of carrier in the cheese, and in the butter and NDM processes. Seven selection and marketing alternatives were investigated to determine the expected differences in economic returns by varying the selection criteria and the end products of value.     

The suitability of milk from a spring-calved dairy herd during the transition from normal to very late lactation for the manufacture of low-moisture Mozzarella cheese

Bulk milk from a spring-calved dairy herd on a good plane of nutrition was collected and made into low-moisture Mozzarella cheese weekly over 10 weeks from 22 September (218 days in lactation, DIL) to 27 November (284 DIL). The 10 weeks were divided into three lactation periods: normal lactation (NL), 218–240 DIL; late lactation (LL), 241–265 DIL; and very late lactation (VLL), 266–284 DIL. Lactation period did not significantly influence milk composition, percentages of milk fat or protein recovered to cheese, cheese yield and composition, primary proteolysis or cooking properties during storage. The curd firmness of the rennet-induced gels from the LL or VLL milk samples was significantly higher than that of the NL milk. The mean firmness over the 40-d storage period of cheese from the LL and VLL milks was significantly higher than that of the cheeses from the NL milk. Satisfactory Mozzarella cheese making characteristics of milk from spring-calved cows can largely be sustained into late lactation by maintaining good dietary nutrition and herd management practices.     

Managing diet quality for Cheddar cheese manufacturing milk. 1. The influence of protein and energy supplements.

The effects of supplementing cows' diets with protein and energy on milk composition and the composition and yield of Cheddar cheese were investigated. This research addresses the problems of seasonal reduction in the capacity of cheese curds to expel moisture as observed in parts of south-eastern Australia. Milk was collected from cows offered a basal diet of silage and hay supplemented with different sources and levels of dietary protein and energy. The protein supplements were sunflower, canola, cottonseed meal and lupin, and the energy supplements were maize grain, oats, wheat and barely. This milk was used to manufacture Cheddar cheese on a pilot scale. Cheese moisture content was dependent on the source and level of dietary protein and energy. Milk from cows offered the lupin protein supplements and wheat energy supplements consistently produced cheese with a lower moisture content and moisture in fat-free matter. Milk from these supplemented diets had increased casein concentrations and higher proportions of alpha S2-casein than milk from the poor quality control diet. Cheese yield was directly related to the total casein concentration of milk, but was not influenced by differences in casein composition. Supplementing the cows' diets increased the inorganic P, Mg and Ca concentrations in milk. A low inorganic P concentration in milk from cows offered the control diet was caused by a low intake of dietary P. These findings showed that changes in the mineral and casein composition of milk, associated with diet, could influence the composition of Cheddar cheese.     

Whole Milk Reverse Osmosis Retentates for Cheddar Cheese Manufacture: Cheese Composition and Yield

The impact of concentrating whole milk by reverse osmosis prior to Cheddar cheese making was studied. Heat treated, standardized, whole milk was reduced in volume by 0, 5, 10, 15, and 20% prior to Cheddar cheese manufacture. Milk solids at various milk volume reductions were 11.98, 12.88, 13.27, 14.17, and 15.05%, respectively. Permeates contained only traces of organic matter and would not create a significant by-product handling problem for a cheese plant. Solids content of the whey from cheese making increased with increasing milk concentration. Proximate compositions of reverse osmosis cheeses were comparable to control cheeses. Fat losses decreased, and fat retained in the cheese increased with increasing milk solids concentration. Improved fat recovery in the cheese was related to the amount of mechanical homogenization of milk fat during the concentration process. Actual, composition adjusted, and theoretical cheese yields were determined. Increased retention of whey solids and improved fat recovery gave cheese yield increases of 2 to 3% above expected theoretical yields at 20% milk volume reduction. Water removal from whole milk prior to Cheddar cheese manufacture gave increased productivity and cheese yield without requiring different cheese-making equipment or manufacturing procedures.     

乳酸菌胞外多糖对低脂Mozzarella奶酪质构、微观结构等品质的影响

目的 研究乳酸菌胞外多糖对低脂Mozzarella奶酪质构等品质特性的影响。方法 在脱除50%乳脂肪的原料中加入0.5%乳酸菌胞外多糖制作低脂Mozzarella奶酪, 同时以全脂Mozzarella奶酪和低脂Mozzarella奶酪为对照, 对Mozzarella奶酪成熟过程中的硬度、弹性、胶黏性、咀嚼性、融化性、油脂析出性、微观结构及感官评分等指标进行分析。结果 0.5%乳酸菌胞外多糖提高了低脂Mozzarella奶酪的水分含量、出品率, 改善了低脂Mozzarella奶酪致密的结构, 形成了类似全脂Mozzarella奶酪疏松、光滑的组织结构, 降低了低脂Mozzarella奶酪的硬度、胶黏性和咀嚼性, 提高了弹性、融化化性和油脂析出, 成熟90 d加入多糖低脂Mozzarella奶酪的滋味和气味、组织状态、色泽接近全脂Mozzarella奶酪。结论 乳酸菌胞外多糖可以提高低脂Mozzarella奶酪的水分含量和出品率, 改善低脂Mozzarella奶酪的组织结构和质构特性。     

Effect of protein-to-fat ratio of milk on the composition, manufacturing efficiency, and yield of cheddar cheese

Twenty-three Cheddar cheeses were prepared from milks with a protein content of 3.66% (wt/wt) and with different protein-to-fat ratio (PFR) in the range 0.70 to 1.15; the PFR of each milk differed by 0.02. For statistical analysis, the 23 cheeses were divided into 3 PFR groups: low (LPFR; 0.70 to 0.85), medium (MPFR; 0.88 to 1.00) and high (HPFR; 1.01 to 1.15), which were compared using ANOVA. The numbers of PFR values in the LPFR, MPFR, and HPFR groups were 9, 7, and 7, respectively. Data were also analyzed by linear regression analysis to establish potentially significant relationships among the PFR and response variables. Increasing PFR significantly increased the levels of cheese moisture, protein, Ca, and P, but significantly reduced the levels of moisture in nonfat substances, fat-in-DM, and salt-in-moisture. The percentage of milk fat recovered in the LPFR cheese was significantly lower than that in the MPFR or HPFR cheeses. In contrast, the recovery of water from milk to the LPFR cheese was significantly higher than that in the MPFR or HPFR cheeses. Increasing the PFR led to a significant decrease in the actual yield of cheese per 100 kg of milk but a significant increase occurred in the normalized yield of cheese per 100 kg of milk with reference values of fat plus protein (3.4 and 3.3%, wt/wt, respectively). The results demonstrate that alteration of the PFR of cheese milk in the range 0.70 to 1.15 has marked effects on cheese composition, component recoveries, and cheese yield.     

不同工艺参数对Mozzarella干酪质构和功能特性的影响

采用三因素二次通用旋转组合设计,研究热缩温度、堆酿pH、拉伸温度等关键工艺参数对全脂Mozzarella干酪的质构特性(硬度、凝聚性、弹性)和功能特性(融化性和油脂析出性)的影响规律,结果表明提高热缩温度可增加干酪硬度及干酪的油脂析出性;提高拉伸温度也可增加干酪的油脂析出性;堆酿pH对干酪的弹性有较大影响,随着堆酿pH的降低,干酪的弹性增大,并与热烫拉伸温度之间有交互作用,即低的堆酿pH和高的拉伸温度时干酪的弹性大。