Effects of lake-basin morphological and hydrological characteristics on the eutrophication of shallow lakes in eastern China

The Yangtze River floodplain contains numerous oxbow or riverine lakes, all of which were openly connected with the Yangtze River or its major tributaries prior to 1950s. However, stresses resulting from human settlement and utilization of catchment resources have exerted great pressures on these lake ecosystems changing their morphology, connectivity and trophic state lakes. This study examined the interaction of these changes and their impact on eutrophication for 90 shallow lakes in eastern China in 2008 to 2011. TN and TP in the study lakes had negative relationships with mean water depth (Z_mean), but no single lake-basin characteristic was found to dominate chlorophyll-a (Chl-a) growth. Instead, water depth and surface area were found to interactively affect Chl-a concentrations in smaller lakes. That is, Chl-a concentration in the lakes with Z_mean > 2 m and surface area (SA) ≤ 25 km² was significantly higher than that in relatively larger lakes with Z_mean > 2 m and SA > 25 km² (p-value ≤ 0.038). Chl-a concentration was higher in the lakes located within the lower Yangtze River basin which had longer retention times, than in the lakes located within the middle Yangtze River basin, where flow velocity is relatively larger. As expected, the water quality was found to be better in the lakes hydraulically connected with rivers than in those isolated from the river. This study revealed that lake-basin morphology and hydrology dominated algal blooms in the highly eutrophic shallow lakes in eastern China.     

Space-for-time proxy for climate change in deep lakes in the Canadian cordillera: Seasonality along a latitudinal climate gradient

A comparison of the annual thermal histories in seven deep, intermontane lakes in western Canada along a north–south transect from 49.67° to 61.25° N reveals latitudinal trends in thermal structure; specifically: 1) summer maximum and winter minimum mean temperatures decrease ~  0.2°C per degree latitude, 2) the dates of maximum and minimum heat content lag ~ 1 and ~  6 days per degree latitude, respectively, 3) the lengths of summer and winter stratification lag ~  8 days per degree latitude, 4) the timing of the spring and autumn turnovers lags ~  4 days per degree latitude, 5) the timing of the onset and break-up of ice cover lags ~ 6 days per degree latitude, and 6) the rate of response to atmospheric heating and cooling is ~  1MJ m^− 2 day^− 1 per degree latitude. We propose that insight into the response of a lake at a specific latitude to climate change can be acquired by examining the present conditions of a morphologically and dynamically similar lake at an appropriate latitude and altitude further south. Of interest is the potential transition of a lake from one mictic state to another, with consequences to the resupply of nutrients to the euphotic zone and to the phenology of spring and fall phytoplankton blooms. The absence or presence of ice cover and timing of freeze-up and break-up also affect a lake's heat budget and thermal history. The full interpretation of climate signals in lake systems requires an understanding of lake-specific physical forcing and response mechanisms.     

Microzooplankton distribution,dynamics, and trophic interactions relative to phytoplankton and quagga mussels in Saginaw Bay,Lake Huron

Invasive quagga mussels have recently replaced zebra mussels as the dominant filter-feeding bivalves in the Great Lakes. This study examined microzooplankton (i.e., grazers < 200 μm) and their trophic interactions with phytoplankton, bacteria, and bivalve mussels in Saginaw Bay, Lake Huron, following the zebra to quagga mussel shift. Microzooplankton distribution displayed strong spatial and temporal variability (1.73–28.5 μg C/L) relative to phytoplankton distribution. Ciliates were the dominant component, especially in the spring and early summer. Rotifers and dinoflagellates increased toward late summer/fall in the inner and outer parts of the bay, respectively. Microzooplankton grazing matched bacterial growth rates and removed ca. 30% of the phytoplankton standing stock in the < 100 μm size fraction per day. The greatest herbivory occurred at the site dominated by colonial cyanobacteria. Microzooplankton, which comprised < 4% of the quagga mussels prey field (i.e. available prey), contributed 77% and 34% to the quagga carbon-based diet during Microcystis and diatom blooms, respectively. Feeding on microzooplankton could buffer mussels during lean periods, or supplement other consumed resources, particularly during noxious cyanobacterial blooms. The results of this study demonstrate that microzooplankton are a resilient and critical component of the Saginaw Bay ecosystem.     

Assessment of Potential Effects of Climate Change on Heavy Lake-Effect Snowstorms Near Lake Erie

The potential effects of future climate change on the frequency of heavy lake-effect snowstorms in the Lake Erie snowbelt were assessed using recent transient simulations from two General Circulation Models (GCMs): the second-generation Hadley Centre (HadCM2) and the first generation Canadian Climate Centre (CGCM1) coupled ocean-atmosphere models. An analysis of historical heavy lake-effect snowstorms identified six weather conditions to be closely related to heavy lake-effect snowstorm occurrence: surface wind speed > 6 m/s, surface wind direction of south southwest to west northwest, surface air temperature in the range of −10°C to 0°C, lake surface to air temperature difference > 7°C, lower tropospheric stability (_{T lake − 850} >15°C), and a highly amplified middle tropospheric wave train. These criteria were applied to daily grid point data from the GCMs for two periods, the late 20^th Century and the late 21^st Century, to determine the relative frequency with which heavy lake-effect conditions were predicted. Surface conditions favorable for heavy lake-effect snow decreased in frequency by 50% and 90% for the HadCM2 and CGCM1, respectively, by the late 21^st Century. This reduction was due almost entirely to a decrease in the number of occurrences of surface air temperature in the range of −10 to 0°C, which in turn was the result of an increase in average winter air temperatures. Other surface conditions favorable for lake-effect snow occurred at about the same frequency in the late 21^st Century as in the late 20^th Century, suggesting that lake-effect rain events may replace lake-effect snow events. Changes in the middle tropospheric wave train were also noted in both models. However, there were sizable biases in the simulation of the present-day climate, raising questions about the validity of the future projections.     

Habitat characteristics of a unionid refuge in the thermal plume of a power plant in western Lake Erie

We examined habitat characteristics to determine why a rich community of unionid mussels lives in the thermal plume of the Bayshore Power Plant, Oregon Ohio. We expected to find that the power plant reduced the density and viability of dreissenid veligers and that the depth and organic matter content of sediments in the thermal outflow were greatest near the plant. Water temperature averaged 3.8 ± 1.5 °C warmer at the plant's outflow than intake, and the ratio of live:dead veligers was significantly lower at the outflow than intake on most dates (P ≤ 0.05). A laboratory experiment confirmed that heating water comparable to passing through the power plant significantly reduced veliger viability (P ≤ 0.01). However, wind direction affected the differences in veliger density between the intake and outflow (P ≤ 0.01), with easterly winds (opposing the direction of discharge) increasing the density of veligers at the outflow. Similarly, water temperature declined with distance from the plant, but east winds increased variations in water temperatures. Apparently wind direction facilitates (westerly) or slows (easterly) the discharge of water from the small embayment receiving the power plant's thermal effluent. Particulate organic matter content of the thermal plume varied with wind speed, apparently due to suspension of sediments in this shallow water. Finally, only the coarse (> 10 mm) size fraction of the benthic sediments was related to distance from the power plant. Thus the thermal regime of this habitat appears to be the primary explanation for this unionid refuge.     

Measurement of Diporeia respiration rate for Lake Superior

The freshwater amphipod Diporeia is a dominant macroinvertebrate species in Lake Superior’s benthic community and an important prey item for many fish. A capacity to predict growth and production rates of Diporeia using a bioenergetics model requires information on physiological processes of the species. The objective of this study is to quantify oxygen consumption of Lake Superior Diporeia and to determine if respiration rate changes with body length. Diporeia were collected from Lake Superior and kept over natural sediment maintained at 4 °C. Dissolved oxygen levels for groups of immature (2 mm), juvenile (4 mm), and adult (6 mm) Diporeia in 20 ml microcosms were measured using a polarographic microelectrode. Mass-specific respiration rates for Lake Superior Diporeia ranged from 32.0 to 44.7 mg O₂ g DW^− 1 day ⁻¹. A significant relationship between body length and mass-specific respiration rate (p > 0.1) was not found. The estimate of Diporeia respiration presented here is significantly higher (p < 0.05) than previous findings from populations in Lakes Michigan and Ontario. This study provides new data on respiration rates of Lake Superior Diporeia and compares findings to studies for other connecting Great Lakes.     

Systemic,early-season Microcystis blooms in western Lake Erie and two of its major agricultural tributaries (Maumee and Sandusky rivers)

Recurrent, massive cyanobacterial blooms composed mainly of the genus Microcystis indicate a broad-scale re-eutrophication of Lake Erie. In the past, ameliorating eutrophication relied on intentionally decreasing point-source tributary nutrient, especially phosphorus, loads to the lake. However, recent research has shown that tributaries load not only nutrients but also bloom-levels of phytoplankton, including Microcystis. We built on this previous work by sampling earlier in the year and in much smaller tributaries in both the Maumee and Sandusky systems. We found Microcystis wet biomasses in these tributaries averaged 3.16 mg/L (± 0.59 mg/L, one standard error of the mean) in 2009 and 3.42 mg/L (± 0.55 mg/L) in 2010. Importantly, we found Microcystis in small ditches in March, much earlier than previously observed. Microcystis biomass did not directly correspond to measured phosphorus, chlorophyll, or phycocyanin concentrations likely reflecting complexities associated with lagged physiological responses and/or non-linear growth relationships. Consequently, our findings emphasize that Microcystis blooms form a more broad-scale problem than previously documented, occurring far upstream much earlier in the year.     

The MERIS Maximum Chlorophyll Index; its merits and limitations for inland water algal bloom monitoring

Satellite remote sensing methods adopting wavelengths in the red and near infra-red have been shown to be superior to the standard blue to green ratio based approaches in the detection of algal blooms under turbid, eutrophic conditions. Here, the MERIS Maximum Chlorophyll Index (MCI) has been explored as a tool for monitoring algal blooms in North America's inland waters where waters range from optically complex, turbid, eutrophic conditions, to low chlorophyll and oligotrophic conditions. Assessment of the MERIS MCI product is made for intense blooms of cyanobacteria in Lake of the Woods, algal blooms in turbid waters of Lake Erie, and low chlorophyll conditions in Lake Ontario. The MCI product is shown to be a versatile tool in monitoring intense surficial algal blooms with chlorophyll concentrations in the 10–300 mg m^? 3 range, while limited in its application to low-biomass conditions as observed in Lake Ontario. Wavelength shifts in the position of the MCI peak for different chlorophyll concentration ranges, as well as variations in the inherent optical properties of water colouring constituents, are anticipated to account for regional variations in MCI–chlorophyll relationships and potentially hinder a universally applicable quantitative MCI product.     

Neonatal Behavioral Assessment Scale Performance in Humans Influenced by Maternal Consumption of Environmentally Contaminated Lake Ontario Fish

Behavioral effects in neonates of their mothers’ consumption of Lake Ontario fish were examined using the Neonatal Behavioral Assessment Scale (NBAS). Newborns of women who had consumed > 40 equivalent pounds of fish were placed in a high-exposure group (n = 152), those of women who had consumed < 40 equivalent pounds of fish were placed in a low-exposure group (n = 243), and offspring of women who reported having never eaten Lake Ontario fish comprised the control group (n = 164). Assessments were given at 12–24 hours after birth, and again at 25–48 hours after birth. Despite relatively low levels of fish consumption, newborns in the high-exposure group scored more poorly than those in both the low-exposure and control groups on the Reflex, Autonomic, and Habituation clusters of the NBAS. No significant group differences were found on the Orientation, Range of State, Regulation of State, or Motor clusters of the NBAS, nor did birth weight or head circumference differ between groups. These results represent the first replication and extension of the neonatal results of the Lake Michigan Maternal Infant Cohort study (Jacobson et al. 1984).     

Sensitivity of Ancient Lake Ohrid to Local Anthropogenic Impacts and Global Warming

Human impacts on the few ancient lakes of the world must be assessed, as any change can lead to an irreversible loss of endemic communities. In such an assessment, the sensitivity of Lake Ohrid (Macedonia/Albania; surface area A = 358 km², volume V = 55 km³, > 200 endemic species) to three major human impacts—water abstraction, eutrophication, and global warming—is evaluated.It is shown that ongoing eutrophication presents the major threat to this unique lake system, even under the conservative assumption of an increase in phosphorus (P) concentration from the current 4.5 to a potential future 9 mg P m⁻³. Eutrophication would lead to a significant reduction in light penetration, which is a prerequisite for endemic, deep living plankton communities. Moreover, a P increase to 9 mg P m⁻³ would create deep water anoxia through elevated oxygen consumption and increase in the water column stability due to more mineralization of organic material. Such anoxic conditions would severely threaten the endemic bottom fauna. The trend toward anoxia is further amplified by the predicted global warming of 0.04°C yr^–1, which significantly reduces the frequency of complete seasonal deep convective mixing compared to the current warming of 0.006°C yr⁻¹. This reduction in deep water exchange is triggered by the warming process rather than by overall higher temperatures in the lake. In contrast, deep convective mixing would be even more frequent than today under a higher temperature equilibrium, as a result of the temperature dependence of the thermal expansivity of water. Although water abstraction may change local habitats, e.g., karst spring areas, its effects on overall lake properties was shown to be of minor importance.     

Native rainbow smelt and nonnative alewife distribution related to temperature and light gradients in Lake Champlain

Alewife (Alosa pseudoharengus) recently became established in Lake Champlain and may compete with native rainbow smelt (Osmerus mordax) for food or consume larval rainbow smelt. The strength of this effect depends partly on the spatial and temporal overlap of different age groups of the two species; therefore, we need a better understanding of factors affecting alewife and rainbow smelt distributions in Lake Champlain. We used hydroacoustics, trawls, and gill nets to document vertical fish distribution, and recorded environmental data during 16 day–night surveys over two years. Temperature, temperature change, and light were all predictors of adult and age-0 rainbow smelt distribution, and temperature and light were predictors of age-0 alewives' distribution (based on GAMM models evaluated with AIC). Adult alewives were 5–30 m shallower and age-0 alewives were 2–15 m shallower than their rainbow smelt counterparts. Adult rainbow smelt distribution overlapped with age-0 rainbow smelt and age-0 alewives near the thermocline (10–25 m), whereas adult alewives were shallower (0–6 m) and overlapped with age-0 alewives and rainbow smelt in the epilimnion. Adult rainbow smelt were in water < 10–12 °C, whereas age-0 rainbow smelt were in 10–20 °C, and adult and age-0 alewives were in 15–22 °C water. Predicting these species distributions is necessary for quantifying the strength of predatory and competitive interactions between alewife and rainbow smelt, as well as between alewife and other fish species in Lake Champlain.     

A Comparison of Photosynthate Allocation in Lakes

We compared the relationships between photosynthate allocation to protein, carbohydrate, lipid and low molecular weight (LMW) fractions and the variables daylength and water temperature in Lakes Huron, Michigan, and Ontario as well as three smaller headwater lakes in the Lake Ontario drainage. In all lakes investigated the allocation of recently produced photosynthate to carbohydrate was strongly related to daylength (% carbohydrate = −3.5 * daylength (hr) + 72.8; n = 59, r² = 0.56). The percentage of photosynthate allocated to protein was a function of water temperature in all lakes although the y-intercept for the protein-temperature relationship was much lower in the three headwater lakes and Lake Ontario (% protein = 0.50 * temperature (°C) + 6.1; n = 37, r² = 0.52) than in Lake Huron and Lake Michigan (% protein = 0.68 * temperature (°C) + 24.2; n = 23, r² = 0.49). The increase in allocation to protein was related to a decrease in allocation to low molecular weight material (% LMW = −1.1 * % protein + 57.13; n = 60, r² = 0.72). The percentages of photosynthate in lipid and LMW material were not related to any of the environmental variables measured. Assuming that photosynthate allocation is related to biochemical composition, the phytoplankton in Lakes Huron and Michigan were more protein rich for a given temperature than those in Lake Ontario and in the smaller inland lakes. The protein deficit was due to an increase in allocation to LMW material.     

Feeding ecology of age-0 lake whitefish in Saginaw Bay,Lake Huron

Age-0 lake whitefish Coregonus clupeaformis (11–160 mm total length) were collected from Saginaw Bay, Lake Huron during April–November 2009 and 2010 for diet analysis and for the evaluation of ontogenetic changes in feeding ecology. Lake whitefish ≤ 50 mm ate mainly zooplankton, after which their diets switched mainly to benthic macroinvertebrates. Cyclopoida were the dominant prey consumed by very small lake whitefish (< 17 mm) and the most frequently selected zooplankton type for individual small fish. Once lake whitefish reached 18–19 mm, Cyclopoida in the diet declined and cladocerans emerged as an important diet item. Daphnia were the most common cladoceran in the diets, but for fish 31–50 mm Bosminidae were also relatively important. Although the shift to Daphnia could represent an ontogenetic point when lake whitefish were large enough to effectively handle this prey, it also took place when the relative availability of Daphnia increased. Once lake whitefish were > 50 mm, Chironomidae larvae became a dominant prey item and this shift to benthivory coincided with a 55% increase in length-adjusted energy content between June and July. However, as fish grew (around 110–120 mm), Sphaeriidae and the benthic zooplankton Chydoridae became increasingly important in the diet. As these less energetically rich prey were incorporated into the diet, there were corresponding 21 and 15% decreases in length-adjusted energy content from July to August and September, respectively.     

Accumulation of Contaminant Metals in the Amphipod Diporeia spp. in Western Lake Ontario

Sediment and amphipods (Diporeia spp.) were collected in areas in western Lake Ontario suspected of containing a range of sediment metal contents. The total metal contents (Al, As, Cd, Cr, Cu, Ni, Fe, V, and Zn) of depurated Diporeia tissues were measured and compared to the total metal contents of the surrounding sediment. The fractional bioavailability of As, Cu, Fe, and Zn was determined in those same sediments using a sequential chemical extraction (SCE) procedure and correlated with depurated Diporeia tissue metal contents. Results of these analyses were then used to assess the ability of Diporeia to accumulate sediment metal contamination. A comparison of metal biota-sediment accumulation factors (BSAFs) and sediment metal enrichment factors (EFs) for each metal yielded separate metal groups showing different behavior for tissue accumulation. Aluminum, Cr, Fe, Ni, and V were not enriched in the sediments and were not accumulated in Diporeia tissues, while As, Cd, Cu, and Zn were enriched in the sediments and accumulated in Diporeia tissues. SCE results showed that Cu (3.2 to 8.4 %) and Zn (13 to 19 %) contents in the easily extractable sediment fractions (MgCl₂ and Na-acetate) were significantly correlated with Cu (r = 0.86; P<0.01) and Zn (r = 0.69; P<0.05) contents in depurated Diporeia tissue. In addition, Cu and Zn in Diporeia tissues are also correlated to the total sediment metal content for each respective metal (Cu: r = 0.67, P < 0.05; Zn: r = 0.66, P < 0.05). The high BSAFs for Cu and Zn, in combination with the association of tissue metal contents with easily extracted sediment metal fractions, suggest that Diporeia may be a potential bioindicator for Cu and Zn contamination in sediments.     

Reproductive Potential and Fecundity of Lake Trout Strains in Southern and Eastern Waters of Lake Ontario, 1977–1994

We assessed the reproductive potential of various genetic strains of hatchery lake trout (Salvelinus namaycush) in southern and eastern Lake Ontario from indices of fecundity and indices of male abundance. Indices were constructed from catches of mature lake trout in gill nets during September 1980 to 1994 after correcting for mortality from sea lampreys (Petromyzon marinus) which occurred between September sampling and late fall spawning. Strain and age were assigned to individual lake trout based on clipped fins and maxillary bones or coded wire tags. Fecundity-length relationships for fish of the same age, determined from mature females collected in 1977 to 1981 and 1994, were not different (P > 0.05) among genetic strains. For all strains combined, fecundity-length relationships in 1977 to 1981 were not different among fish of various ages but in 1994, age-5 and -6 fish had fewer eggs (P < 0.003) than age-7 fish, and age-7 fish had fewer eggs (P < 0.003) than fish of age 8, 9, or 10. Annual indices of fecundity varied 19 fold and indices of mature males varied 11 fold; both indices were low in the early 1980s, increased sharply in the mid 1980s, and peaked in 1993. The strain which dominated fecundity and mature male indices shifted during the study from Seneca Lake strain to Lake Superior strain and then back to Seneca Lake strain. However, changes in either reproductive potential or genotypes do not appear responsible for the abrupt appearance of naturally-produced yearling lake trout throughout southern and eastern Lake Ontario in 1994–1995, the first widespread occurrence of juveniles produced by hatchery lake trout in Lake Ontario.     

Distribution of Metals in Different Size Fractions of Sediment from the Niagara River

This study was carried out to demonstrate that the concentration of major and trace elements, determined in sediment samples separated mechanically into different size fractions, gives better information for assessing a potential hazard of in situ, dredged, or resuspended sediments than the bulk total concentration. The concentrations of major elements (Si, Al, Ca, Mg, Na, K, Fe, Ti, Mn, and P), trace elements (Cr, Co, Cu, Zn, V, Ni, and Pb), and organic and carbonate C were determined in six size fractions (< 13 μm, 13–19 μm, 19–27 μm, 27–40 μm, 40–54 μm, and 54–150 μm) of bottom sediments collected at eight stations along the Niagara River. Fine particles (< 13 μm) separated from the river sediments exposed to pollution sources accumulated greater metal quantities than particles in the other size fractions. The contribution of trace elements from specific size fractions was calculated from the particle size distribution and trace elements concentration. With the exception of Ni, significant differences were found between trace elements concentration in specif ic particle size fractions of Niagara River sediments and that from the nearshore zone of Lake Erie.     

Evidence supporting the importance of nutrient regeneration by nano- and micrograzers for phytoplankton photosynthesis in Lake Malawi/Nyasa

Photosynthesis and nutrient status measurements were compared in size fractioned water samples collected in southern Lake Malawi in three different seasons. The size fractions analyzed were 0–2.0 μm, 0–20 μm, and 0–200 μm. Total chlorophyll concentrations were relatively invariant at about 1 μg L^?1 in all seasons (wet stratified, deep mixing, dry stratified). Over 90% of total chlorophyll was < 20 μm and 40–50% < 2 μm. Stoichiometric ratios of carbon (C):nitrogen (N) and C:phosphorus (P) for the < 2-μm seston were similar to < 20-μm and < 200-μm seston samples and indicated that all size fractions were moderately N and P deficient in all seasons. N and P uptake experiments demonstrated that when the < 2-μm picoplankton were isolated from the larger sizes, they showed high N and P nutrient debts, apparently in response to the removal of nano- and micrograzers. This effect was strongest for N debt in the dry stratified season, but was apparent for P debt in all seasons. The chlorophyll-normalized light saturated rate of photosynthesis, P^b_m, was always higher in whole water samples compared to the rates of the isolated picophytoplankton which were lowest in the dry season. We infer that nano- and micrograzers were an especially important source of regenerated nutrients when the lake was stratified although their removal as a source of nutrient to the picophytoplankton may affect P^b_m in all seasons. Nitrogen regeneration was especially critical to the picophytoplankton in the dry stratified season but less important in the windy deep mixing season.     

Precision of Ages Determined from Scales and Opercles for Yellow Perch Perca flavescens

Scales and opercles were used to age yellow perch Perca flavescens collected in 1989 from Lake Madison (South Dakota), Dauphin Lake (Manitoba), and southern Lake Michigan (Indiana). Three readers aged fish from Lake Madison and Dauphin Lake once and two readers aged fish from Lake Michigan twice. The coefficient of variation (CV) was calculated to compare precision. Ages determined from opercles were as precise as those from scales for fish from Lake Madison (CV = 0 for both structures), and more precise than ages from scales for fish from Dauphin Lake (CV_opercle = 14.0, CV_scale = 27.4, p < 0.001) and Lake Michigan (CV _opercle = 10.6, CV_scale = 13.9, p < 0.001). The high precision of scale and opercle ages for yellow perch from Lake Madison can be attributed to the fast growth rate of fish from that lake and also that only age 1 and 2 fish were aged. The greater precision of opercle ages in comparison to scale ages for Dauphin Lake and Lake Michigan yellow perch can be attributed to ease of recognition of false annuli on opercles as well as to difficulty in distinguishing between false and true annuli crowded on the edge of scales from mature, slower growing fish. Because true annuli are more easily recognized on opercles, ages determined from opercles may be more accurate than ages determined from scales for yellow perch growing at slow or moderate rates.     

Contaminant Concentrations and Reproductive Rate of Lake Superior Bald Eagles, 1989–2001

We investigated the trend in contaminant concentrations in Lake Superior bald eagles (Haliaeetus leucocephalus) from 1989–2001, and examined the relationship of contaminant concentrations to eagle reproductive rate during that time. Concentrations of dichloro-diphenyl-dichloroethylene (DDE) and total polychlorinated biphenyls (PCBs) in nestling blood plasma samples decreased significantly from 1989-2001 (p = 0.007 for DDE, p = 0.004 for total PCBs). Mean contaminant concentrations in eaglet plasma, 21.7 μg/kg DDE (n=51) and 86.7 μg/kg total PCBs (n = 54), were near or below the estimated threshold levels for impairment of reproduction as determined in other studies. A preliminary assessment of polybrominated diphenyl ether (PBDE) concentrations indicated a mean of 7.9 μg/kg total PBDEs in Lake Superior eaglet plasma (n = 5). The number of occupied bald eagle nests along the Wisconsin shore of Lake Superior increased from 15 to 24 per year, between 1989 and 2001 (p < 0.001, r² = 0.70, n = 13 years). Eagle reproductive rate did not increase or decrease significantly between 1989 and 2001 (p = 0.530, r² = 0.037, n = 13 years, mean productivity = 0.96 young per occupied nest). The lack of correlation between reproductive rate and contaminant concentrations, as well as the comparison of contaminant concentrations to the estimated thresholds for impairment of reproduction, suggest that DDE and PCBs no longer limit the reproductive rate of the Lake Superior eagle population in Wisconsin.