Content of zinc,iron and their absorption inhibitors in Nicaraguan common beans (Phaseolus vulgaris L.)

Common bean (Phaseolus vulgaris L.), the staple crop of Nicaragua, provides protein and nonhaem iron, but inhibitors such as phytate may prevent absorption of iron and zinc by the consumer. Warehouses in Nicaragua do not have controlled atmospheres, so beans are exposed to high temperatures and humidities that may accelerate quality loss. To evaluate the impact of 6 months of storage on quality, four national accessions of common bean were submitted to two treatments, a conventional warehouse with uncontrolled temperature and humidity, and accelerated ageing at 40 °C and 75% RH. Iron content was 61–81 mg/kg of which 3–4% was bioavailable, and zinc content was 21–25 mg/kg, of which 10–12% was bioavailable. Bioavailability generally increased in storage, significantly so in year-old INTA Linea 628 in accelerated ageing. The concentration of phytate was 8.6–9.6 mg/g and it contained 54–63% of the total phosphorus. Improvement in bioavailability of divalent cations is needed.     

Natural occurrence of free anthocyanin aglycones in beans (Phaseolus vulgaris L.)

The application of an improved method of extraction and purification has allowed the characterization of anthocyanins and free anthocyanidins in beans, Phaseolus vulgaris L. using LC-MS determination.     

Protein and starch content of raw,soaked and cooked beans (Phaseolus vulgaris L.)

Organoleptic qualities are an important factor in the market value of legumes, especially in developed countries. Unfortunately, the molecules that have the greatest influence on the texture of beans undergo important transformations during soaking and cooking. Moreover, the extent to which these changes are linear is unknown, making uncertain the use of raw beans in chemical screenings for sensory properties. Results of our experiments show that the amount of protein and amylose present in raw beans provides a good indicator of these substances in cooked beans (correlation coefficients between raw and cooked beans = 0.91, p ? 0.001 and 0.87, p ? 0.01, respectively). The Mg content in the raw seed coat also shows a strong correlation with that found in the cooked seed coat (r = 0.86, p ? 0.01). The correlations found for the other traits are weaker, indicating that the evaluation of raw samples is not predictive of the findings in cooked beans.     

Quality of spaghetti pasta containing Mexican common bean flour (Phaseolus vulgaris L.)

The objective of this research was to study the effect of the addition of common bean flour to semolina on the cooking quality and total phenolic content of pasta. Pasta was obtained at three temperatures (60, 70 and 80 °C) and two levels of added common bean flour (15% and 30%); plain pasta (100% semolina) was used as control. Moisture, optimal cooking time, cooking loss, water absorption capacity, colour change, firmness and total phenolic and furosine contents were measured. The cooking time and water absorption were diminished in spaghetti pasta with added common bean flour; cooking loss increased and firmness decreased as a function of the bean flour percentage. A linear relationship between colour change and common bean flour content in pasta was found. Increases of furosine and phenolic contents in pasta with the addition of bean flour were observed.     

Physical and functional characteristics of selected dry bean (Phaseolus vulgaris L.) flours

Many varieties of dry beans (Phaseolus vulgaris L.) are available with entirely different physico-chemical and sensory characteristics. Selected dry bean varieties (red kidney, small red kidney, cranberry and black) were processed into flour and analyzed for the physico-chemical and functional characteristics. The bulk density of the beans flours varied significantly (p < 0.05) from 0.515 g/ml for black bean flour to 0.556 g/ml for red kidney bean flour. The small red kidney bean flour had the highest water absorption capacity (2.65 g/g flour) while black bean flour showed the lowest at 2.23 g/g flour. Significant differences were observed for oil absorption capacities of bean flours, which ranged from 1.23 g/g for small red kidney bean flour to 1.52 g/g for red kidney bean flour. The bean flours emulsion capacity and stability and foaming capacity and stability also varied significantly and was variety-dependent. The highest apparent viscosity, 0.462 Pa.s, was recorded for small red kidney bean flour whereas black bean flour exhibited the lowest value of 0.073 Pa.s at 30 g/100 ml water content in the flour dispersions. The force-deformation curves for doughs from different bean flours showed that black bean flour had the highest peak force or hardness value of 90.7 N followed by doughs from cranberry, small red kidney and red kidney bean flours. The results of this study offer useful data on bean flours' potential uses in different food products.     

Effect of pressure cooking on the antioxidant activity of extracts from three common bean (Phaseolus vulgaris L.) cultivars

Common bean is a traditional legume that is becoming more attractive because of its dietary and associated health benefits. Flor de Mayo M38, Pinto Villa and Bayo Victoria, three common bean cultivars developed in Mexico, were tested for phenol content and free radical scavenging activity before and after autoclaving. Independent analysis of seed coat and cotyledon was performed for each cultivar. Longer cooking times enhanced diffusion of phenols from seed coats to cooking water and from there to cotyledons. Cooking waters showed a remarkable activity similar to crude seed coats extracts although their phenol content was 80% lower.     

Genetic and environmental effects on chemical composition related to sensory traits in common beans (Phaseolus vulgaris L.)

The chemical composition determines plant-derived foods’ sensory traits; genetic and environmental effects and their interactions determine chemical composition. Understanding the importance of each factor should help characterise foodstuffs according to the variety and place of cultivation. We studied the effects of variety (five landraces + two checks) and environment (five locations and two years) on some molecules important for beans’ (Phaseolus vulgaris L.) sensory value. The greatest location effects were on protein and starch content and variety effects were due to combinations of traits (mainly sucrose, protein, and malic acid). The variety × location interactions detected in protein, sucrose, citric acid, and malic acid were difficult to associate to the gastronomic success of a particular variety cultivated at a particular location. Our results showed that protected designation of origin (PDO) in beans could be delimited on the basis of chemical composition, derived from the particular environmental characteristics of a region and the genetic characteristics of the varieties admitted.     

The polyphenolic profiles of common bean (Phaseolus vulgaris L.)

Based on the phenolic profiles obtained by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS), 24 common bean samples, representing 17 varieties and 7 generic off-the-shelf items, belonging to ten US commercial market classes can be organized into six different groups. All of them contained the same hydroxycinnaminic acids, but the flavonoid components showed distinct differences. Black beans contained primarily the 3-O-glucosides of delphinidin, petunidin and malvidin, while pinto beans contained kaempferol and its 3-O-glycosides. Light red kidney bean contained traces of quercetin 3-O-glucoside and its malonates, but pink and dark red kidney beans contained the diglycosides of quercetin and kaempferol. Small red beans contained kaempferol 3-O-glucoside and pelargonidin 3-O-glucoside, while no flavonoids were detected in alubia, cranberry, great northern, and navy beans. This is the first report of the tentative identification of quercetin 3-O-pentosylhexoside and flavonoid glucoside malonates, and the first detailed detection of hydroxycinnamates, in common beans.     

Effects of micronization pretreatments on the physicochemical properties of navy and black beans (Phaseolus vulgaris L.)

Tempering pretreatments were evaluated for the micronization of navy and black beans. Navy and black beans were tempered to 28 g/100 g and 26 g/100 g moisture, respectively, using water, a mixture of salts of bicarbonate, carbonate and phosphate, a mixture of citric acid and ascorbic acid, or ethylenediaminetetraacetic acid (EDTA) and monitored for texture after cooking, and changes in starch, protein and colour. The mixture of sodium bicarbonate, sodium carbonate and dibasic sodium phosphate was more effective than the mixture of citric and ascorbic acid or disodium EDTA or water alone in reducing the hardness of micronized black beans, while the water and salt mixture were equally effective in reducing the hardness of navy beans and the firmness of both navy and black beans. Lower soluble protein levels and higher levels of gelatinized starch were significantly correlated with a decrease in the hardness of micronized beans. Colour was affected for all micronized black beans, but the colour of micronized navy beans was only affected by the acid treatment. The ability of the bean to imbibe and distribute water in its seed renders seed constituents more sensitive to the effect of heat and solutes, thereby influencing the tempering strategy.     

Effect of the cooking on physicochemical and starch digestibility properties of two varieties of common bean (Phaseolus vulgaris L.) grown under different water regimes

Growing and cooking conditions influence the quality and nutritional value of beans. The objective of this research was to determine the effect of cooking on digestibility and physicochemical properties of two varieties of bean grown under different water regimes. Black 8025 and Pinto Durango varieties were grown in irrigated and temporal (rain fed) conditions in two locations of Guanajuato, Mexico. The pasting profiles of the cooked beans showed a significant decrease in viscosity. The enthalpy of the raw and cooked beans ranged from 2.75 to 3.95 and 0.62 to 0.97 J/g, respectively. The percentage of rapidly digestible starch and slowly digestible starch increased, while the percentage of resistant starch was lower in cooked samples. Black 8025 beans had lower glycemic index than Pinto Durango, but no significant difference (P < 0.05) was noted between water regimes. The variety of bean had a more pronounced effect on digestibility properties than the water regime.     

In vitro and in vivo protein hydrolysis of beans (Phaseolus vulgaris) genetically modified to express different phaseolin types

Experiments were conducted to study whether phaseolin type could influence proteolysis susceptibility and nutritional value of total bean protein. The DOR-390 bean cultivar was genetically modified to express different phaseolin types (S, T or I). Beans were soaked and autoclaved. A sequential hydrolysis was carried out in vitro with pepsin and pancreatin. Differences in the degree of protein hydrolysis among bean lines started at 30 min and remained until 240 min, with the S bean proteins presenting lower values (P < 0.05). Subsequently, rats were fed with diets containing beans expressing different phaseolin types as the only source of protein for N digestibility and nutritional value determination. No differences (P > 0.05) in ileal protein digestibility and rat growth were observed. In conclusion, the differences in in vitro hydrolysis between bean lines expressing different phaseolin types had no consequences on growth and N retention in rats.     

Content of antinutritional factors and HCl-extractability of minerals from white bean (Phaseolus vulgaris) cultivars: Influence of soaking and/or cooking

White bean seeds were subjected to soaking in distilled water for 1, 2 and 3 days. In order to perform complete processing, the seeds were cooked until soft. Effects of soaking and/or cooking of white bean seeds on antinutritional factors, mineral contents and HCl-extractability were studied. Phytic acid and polyphenol contents of all cultivars were reduced. Soaking alone and soaking, followed by cooking, reduced mineral contents of the cultivars, but HCl-extractability was significantly (P ? 0.01) improved to varying extents, depending on cultivar type. Soaking and/or cooking treatment was thus found to be an effective technique and caused further improvement in the availability of both major and trace minerals in white bean.     

Shelf stability and sensory properties of flour tortillas fortified with pinto bean (Phaseolus vulgaris L.) flour: Effects of hydrocolloid addition

To flour tortillas formulations containing 25 g/100 g of pinto bean flour, 0.5 g/100 g and 0.75 g/100 g of guar gum and sodium carboxymethyl cellulose (CMC) were added and their shelf stability was studied at 4 and 25 °C over 7 days. Texture, determined instrumentally, rollability, and water holding capacity were the main parameters studied. Selected samples were evaluated by 55 participants to determine consumer acceptability. Firmness and cohesiveness were negatively affected by the addition of bean flour, however, this effect was partially overcome by the addition of hydrocolloids. Guar gum had a positive significant influence on water holding capacity and texture over time (P < 0.001), while CMC had no positive effects. Despite the instrumental texture data, which showed that bean tortillas had inferior attributes than the wheat control, consumers found the overall texture and acceptability of bean tortillas with and without guar gum on the range of “like very much” and “like moderately”, which was significantly higher than the wheat control (P < 0.01). Based on physical and sensorial properties it would appear that these foods are industrially feasible and highly acceptable by health-conscious consumers.     

Effect of cooking and sterilisation on the composition of amino acids in immature seeds of flageolet bean (Phaseolus vulgaris L.) cultivars

The aim of the work was to evaluate the amino acid content and protein quality in products of immature bean seeds with a dry matter content of 40%. The investigation included raw seeds, fresh seeds after cooking to consumption consistency and seeds after canning. Raw seeds of the cultivar Alamo contained more amino acids in 100 g fresh matter than the seeds of the cultivars Flaforte and Mona. Cooking and sterilisation brought about decreases in the content of all the amino acids in the investigated cultivars; 1–50% and 9–60% respectively. Higher losses being noted in sterilised seeds. If the results were expressed in 16 g N, the differences in amino acid content were much smaller. The amino acid content of Mona was lower than that of the remaining cultivars. The cooking of seeds did not bring about changes in relation to the raw material (except tyrosine which showed a decrease 35–45%); however, sterilisation affected the content of most amino acids (from −45 to +17%). Compared with the standard (FAO/WHO/UNU, 2007), the limiting amino acid was cystine with methionine (amino acid score 70–83). Of the seeds prepared for consumption, sterilised bean seeds had a higher EAA index value, while of the cultivars, the seeds of Alamo had the highest value (147–162).     

Effect of cooking and germination on phenolic composition and biological properties of dark beans (Phaseolus vulgaris L.)

Legumes are the base´s diet in several countries. They hold a high nutritional value, but other properties related to human health are nowadays being studied. The aim of this work was to study the influence of processes (boiling or germination) on the phenolic composition of dark beans (Phaseolus vulgaris L. c.v. Tolosana) and their effect on their antioxidant, neuroprotective and anticancer ability. Phenolic composition of raw and processed dark beans was analysed by HPLC-PAD and HPLC–ESI/MS. The antioxidant activity was evaluated by ORAC. Astrocytes cultures (U-373) have been used to test their neuroprotective effect. Anticancer activities were evaluated on three different cell lines (renal adenocarcinoma (TK-10), breast adenocarcinoma (MCF-7) and melanoma (UACC-62)) by sulphorhodamine B method. Qualitative and quantitative differences in phenolic composition have been observed between raw and processed dark beans that influence the antioxidant activity, mainly for germinated samples which show a decrease of antioxidant capacity. Although every assayed extracts decreased reactive oxygen species release and exhibited cytotoxicity activities on cancer cell lines, raw beans proved to be the most active in neuroprotective and antitumoral effects; this sample is especially rich in phenolic compounds, mainly anthocyanins. This study further demonstrated that phenolic composition of dark beans is related with cooking process and so with their neuroprotective and anticancer activity; cooking of dark beans improves their digestion and absorption at intestinal level, while maintaining its protective ability on oxidative process at cellular level.     

Chemical analysis of French beans (Phaseolus vulgaris L.) by headspace solid phase microextraction (HS-SPME) and simultaneous distillation/extraction (SDE)

Headspace Solid Phase Microextraction (HS-SPME) involving divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fibre and simultaneous distillation/extraction (SDE) techniques were applied to study the volatile and semi volatile compounds of thawed and cooked Phaseolus vulgaris L. A total of 104 compounds were detected by GC and GC/MS. Thereof, 76 compounds were identified for the first time in this species. The major differences between HS-SPME and SDE were found in the content of identified alcohols (23.62% SDE versus 62.20% SPME), terpenoids (39.15% SDE versus 2.45% SPME), heterocyclic compounds (13.78% SDE versus 1.21% SPME), hydrocarbons (2.22% SDE versus 13.87% SPME) and esters (0.98% SDE versus 12.98% SPME). The SPME technique was found to be useful for rapid and routine quality controls of thawed French beans, while SDE is favourable to study the entire set of flavour volatiles in the corresponding cooked samples.     

Emulsifying and foaming properties of Phaseolus vulgaris and coccineus proteins

Protein isolates from two Phaseolus cultivars, common bean (Phaseolus vulgaris L.) and scarlet runner bean (Phaseolus coccineus L.), were prepared by wet extraction methods (isoelectric precipitation – 4000 rpm, ultrafiltration, extraction with NaCl 2%, and isoelectric precipitation – 9900 rpm). The protein isolates were characterized by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then evaluated for their solubility. The emulsion stability of emulsions produced at pH 7.0 and 5.5 with 1% or 2% or 3% w/v protein isolate was evaluated by average droplet size diameter, viscosity and creaming measurements. Emulsions with 1% protein content were unstable through storage. Emulsions with 3% w/v protein isolate concentration, extracted by ultrafiltration at pH 5.5 from both cultivars, were flocculated; this was more pronounced for coccineus isolates. The foaming properties, for the respective foams, were investigated. Foams with 1% w/v protein showed little foaming ability Ultrafiltration isolates produced more foam, which was especially stable at pH 5.5.     

Functional properties of three common bean (Phaseolus vulgaris) cultivars stored under accelerated conditions followed by extrusion

Flor de Mayo M38, Pinto Villa and Bayo Victoria beans were stored under accelerated conditions for aging at 40 °C and 60% RH for 3 months. Following adjustment of moisture to 24, 26 and 28 g water/100 g solids, they were extruded at 165, 185 and 205 °C at a constant screw speed. Water absorption index (WAI) and bulk density of extrudates were measured. Feed humidity did not affect the evaluated properties. Storage conditions, temperature and cultivar were the parameters that influenced the evaluated properties. Higher value for WAI was obtained in aged beans, independently of cultivar, temperature and feed humidity used. The bulk density was similar for aged and not aged beans, but at the highest temperature it was lower for aged beans. This fact indicates that bean storing and interaction of temperature have important influence on the final quality of extrudates.