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1.
Mohammad Vahedi Fatemeh Shokrolahi Jalal Barzin Parvin Shokrollahi Leila Taghiyar Mohammad Kazemi Ashtiani 《大分子材料与工程》2020,305(4)
Despite excellent processing and biological properties of gelatin for use as a cell carrier, none of the gelatin‐based hydrogel cell carriers reported to date offer all characteristics including quick formation, injectability, self‐healing, and durability, which are simultaneously required for an ideal system. Here, a gelatin‐based hydrogel with dynamic Schiff base linkages, so‐called “dynamic hydrogel,” as an injectable cell carrier consisting of gelatin and amylopectin multiple aldehyde (AMPA), with all the required characteristics is reported. Biocompatibility and osteoinductivity of the hydrogel are verified through the culture of human bone marrow‐derived mesenchymal stem cells (hBMSCs). As live/dead results show, hBMSCs are alive and highly viable ≈85–90% within the hydrogel after 5 days. According to bromodeoxyuridine cell proliferation assay, a significant increase in the number of the cells seeded in the hydrogel confirms its clinical significance for cell therapy. Most importantly, histological visualization using Mason's Trichrome staining indicates secretion of extracellular matrix around the cells loaded in the hydrogel and also expression level evaluation of the crucial osteogenic markers, confirms that the hydrogel can provide osteoinductive support for osteocyte differentiation of hBMSCs after 14 days. Therefore, this hydrogel provides more progress on the path toward bone tissue engineering and further treatment of bone diseases. 相似文献
2.
During tissue morphogenesis and homeostasis, cells experience various signals in their environments, including gradients of physical and chemical cues. Spatial and temporal gradients regulate various cell behaviours such as proliferation, migration, and differentiation during development, inflammation, wound healing, and cancer. One of the goals of functional tissue engineering is to create microenvironments that mimic the cellular and tissue complexity found in vivo by incorporating physical, chemical, temporal, and spatial gradients within engineered three-dimensional (3D) scaffolds. Hydrogels are ideal materials for 3D tissue scaffolds that mimic the extracellular matrix (ECM). Various techniques from material science, microscale engineering, and microfluidics are used to synthesise biomimetic hydrogels with encapsulated cells and tailored microenvironments. In particular, a host of methods exist to incorporate micrometer to centimetre scale chemical and physical gradients within hydrogels to mimic the cellular cues found in vivo. In this review, we draw on specific biological examples to motivate hydrogel gradients as tools for studying cell-material interactions. We provide a brief overview of techniques to generate gradient hydrogels and showcase their use to study particular cell behaviours in two-dimensional (2D) and 3D environments. We conclude by summarizing the current and future trends in gradient hydrogels and cell-material interactions in context with the long-term goals of tissue engineering. 相似文献
3.
Raúl Sanz-Horta Ana Matesanz Jos Luis Jorcano Diego Velasco Pablo Acedo Alberto Gallardo Helmut Reinecke Carlos Elvira 《International journal of molecular sciences》2022,23(8)
Fibrin hydrogels are one of the most popular scaffolds used in tissue engineering due to their excellent biological properties. Special attention should be paid to the use of human plasma-derived fibrin hydrogels as a 3D scaffold in the production of autologous skin grafts, skeletal muscle regeneration and bone tissue repair. However, mechanical weakness and rapid degradation, which causes plasma-derived fibrin matrices to shrink significantly, prompted us to improve their stability. In our study, plasma-derived fibrin was chemically bonded to oxidized alginate (alginate di-aldehyde, ADA) at 10%, 20%, 50% and 80% oxidation, by Schiff base formation, to produce natural hydrogels for tissue engineering applications. First, gelling time studies showed that the degree of ADA oxidation inhibits fibrin polymerization, which we associate with fiber increment and decreased fiber density; moreover, the storage modulus increased when increasing the final volume of CaCl2 (1% w/v) from 80 µL to 200 µL per milliliter of hydrogel. The contraction was similar in matrices with and without human primary fibroblasts (hFBs). In addition, proliferation studies with encapsulated hFBs showed an increment in cell viability in hydrogels with ADA at 10% oxidation at days 1 and 3 with 80 µL of CaCl2; by increasing this compound (CaCl2), the proliferation does not significantly increase until day 7. In the presence of 10% alginate oxidation, the proliferation results are similar to the control, in contrast to the sample with 20% oxidation whose proliferation decreases. Finally, the viability studies showed that the hFB morphology was maintained regardless of the degree of oxidation used; however, the quantity of CaCl2 influences the spread of the hFBs. 相似文献
4.
Chengqi Lyu Chunyan Li Jiayue Sun Lin Teng Yushi He Derong Zou Jiayu Lu Dan Li 《大分子材料与工程》2021,306(1):2000535
Graphene has attracted great interest for its uses in tissue regeneration. Multilayered graphene hydrogel (MGH) membranes show great potential as barrier membranes in guided bone regeneration. However, when using the conventional methyl methacrylate-embedding process, the MGH membrane swells and loses its natural form, which seriously limits the observation of bone regeneration. A rapid embedding method is developed by adding the cross-linker tetra(ethylene glycol)diacrylate and increasing the polymerization temperature (45 °C) and initiator concentration (64% benzoyl peroxide in penetration liquid). By the rapid embedding method, the shape and properties of the MGH membrane can be well preserved, and the bone tissue can also be well embedded. The resulting blocks have uniform hardness, which makes it possible to obtain thin sections using a cutting and grinding procedure. The rapid embedding method has no significant effect on the conventional methods of bone regeneration detection. The interface between the MGH membrane and new bone tissue can be clearly observed. This new hard-tissue-embedding method can lead to better observation of the interaction between bone and graphene, providing an optimized experimental technology for the study of hard tissue regeneration with graphene in the future. 相似文献
5.
Juan Liu Huaiyuan Zheng Patrina S. P. Poh Hans-Günther Machens Arndt F. Schilling 《International journal of molecular sciences》2015,16(7):15997-16016
Hydrogels are commonly used biomaterials for tissue engineering. With their high-water content, good biocompatibility and biodegradability they resemble the natural extracellular environment and have been widely used as scaffolds for 3D cell culture and studies of cell biology. The possible size of such hydrogel constructs with embedded cells is limited by the cellular demand for oxygen and nutrients. For the fabrication of large and complex tissue constructs, vascular structures become necessary within the hydrogels to supply the encapsulated cells. In this review, we discuss the types of hydrogels that are currently used for the fabrication of constructs with embedded vascular networks, the key properties of hydrogels needed for this purpose and current techniques to engineer perfusable vascular structures into these hydrogels. We then discuss directions for future research aimed at engineering of vascularized tissue for implantation. 相似文献
6.
Monica Boffito Emilia Gioffredi Valeria Chiono Stefano Calzone Elia Ranzato Simona Martinotti Gianluca Ciardelli 《Polymer International》2016,65(7):756-769
Poloxamer P407 (P407) is a Food and Drug Administration approved triblock copolymer; its hydrogels show fast dissolution in aqueous environment and weak mechanical strength, limiting their in vivo application. In this work, an amphiphilic poly(ether urethane) (NHP407) was synthesized from P407, an aliphatic diisocyanate (1,6‐hexanediisocyanate) and an amino acid derived diol (N‐Boc serinol). NHP407 solutions in water‐based media were able to form biocompatible injectable thermosensitive hydrogels with a lower critical gelation temperature behavior, having lower critical gelation concentration (6% w/v versus 18% w/v), superior gel strength (G′ at 37 °C about 40 000 Pa versus 10 000 Pa), faster gelation kinetics (<5 min versus 15–30 min) and higher stability in physiological conditions (28 days versus 5 days) compared to P407 hydrogels. Gel strength and PBS absorption at 37 °C increased whereas dissolution rate (in phosphate‐buffered saline (PBS) at 37 °C) and permeability to nutrients (studied using fluorescein isothiocyanate–dextran model molecule) decreased as a function of NHP407 hydrogel concentration from 10% to 20% w/v. By varying the concentration, NHP407 hydrogels were thus prepared with different properties which could suit specific applications, such as in situ drug/cell delivery or bioprinting of scaffolds. Moreover, deprotected amino groups in NHP407 could be exploited for the grafting of bioactive molecules obtaining biomimetic hydrogels. © 2016 Society of Chemical Industry 相似文献
7.
Pawe&#x; Dec Andrzej Modrzejewski Andrzej Pawlik 《International journal of molecular sciences》2023,24(1)
The treatment of bone defects remains one of the major challenges in modern clinical practice. Nowadays, with the increased incidence of bone disease in an aging population, the demand for materials to repair bone defects continues to grow. Recent advances in the development of biomaterials offer new possibilities for exploring modern bone tissue engineering strategies. Both natural and synthetic biomaterials have been used for tissue repair. A variety of porous structures that promote cell adhesion, differentiation, and proliferation enable better implant integration with increasingly better physical properties. The selection of a suitable biomaterial on which the patient’s new tissue will grow is one of the key issues when designing a modern tissue scaffold and planning the entire treatment process. The purpose of this article is to present a comprehensive literature review of existing and novel biomaterials used in the surgical treatment of bone tissue defects. The materials described are divided into three groups—organic, inorganic, and synthetic polymers—taking into account current trends. This review highlights different types of existing and novel natural and synthetic materials used in bone tissue engineering and their advantages and disadvantages for bone defects regeneration. 相似文献
8.
Luciana Sartore Chiara Pasini Stefano Pandini Kamol Dey Marco Ferrari Stefano Taboni Harley H. L. Chan Jason Townson Sowmya Viswanathan Smitha Mathews Ralph W. Gilbert Jonathan C. Irish Federica Re Piero Nicolai Domenico Russo 《International journal of molecular sciences》2022,23(9)
A great promise for tissue engineering is represented by scaffolds that host stem cells during proliferation and differentiation and simultaneously replace damaged tissue while maintaining the main vital functions. In this paper, a novel process was adopted to develop composite scaffolds with a core-shell structure for bone tissue regeneration, in which the core has the main function of temporary mechanical support, and the shell enhances biocompatibility and provides bioactive properties. An interconnected porous core was safely obtained, avoiding solvents or other chemical issues, by blending poly(lactic acid), poly(ε-caprolactone) and leachable superabsorbent polymer particles. After particle leaching in water, the core was grafted with a gelatin/chitosan hydrogel shell to create a cell-friendly bioactive environment within its pores. The physicochemical, morphological, and mechanical characterization of the hybrid structure and of its component materials was carried out by means of infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, and mechanical testing under different loading conditions. These hybrid polymer devices were found to closely mimic both the morphology and the stiffness of bones. In addition, in vitro studies showed that the core-shell scaffolds are efficiently seeded by human mesenchymal stromal cells, which remain viable, proliferate, and are capable of differentiating towards the osteogenic phenotype if adequately stimulated. 相似文献
9.
So far, several methods for myocardial tissue engineering have been developed to regenerate myocardium and even create contractile heart muscles. Among these approaches, hydrogel based methods have attracted much attention due to their ability to mimic the architecture of native extracellular matrix. Injectable hydrogels are a specific class of hydrogels which can be formed in situ by physical and/or chemical crosslinking. Generally, using these hydrogels is more advantageous because they are minimally (less) invasive in comparison with open surgery. Moreover, with respect to the fact that ‘myocardium is a conductive tissue’, utilization of conductive polymers for myocardial tissue engineering has demonstrated promising results. Both the injectable hydrogels and conductive polymers have some merits and demerits, but studies show that using a combination of them has prominently enhanced regeneration of the myocardium. In this review, the focus is on injectable hydrogels, conductive polymers and injectable conductive hydrogels for myocardial tissue engineering. © 2018 Society of Chemical Industry 相似文献
10.
Emine Alarcin Ayca Bal-
ztürk Hüseyin Avci Hamed Ghorbanpoor Fatma Dogan Guzel Ali Akpek Gzde Yesiltas Tuba Canak-Ipek Meltem Avci-Adali 《International journal of molecular sciences》2021,22(11)
Traumatic injuries, tumor resections, and degenerative diseases can damage skeletal muscle and lead to functional impairment and severe disability. Skeletal muscle regeneration is a complex process that depends on various cell types, signaling molecules, architectural cues, and physicochemical properties to be successful. To promote muscle repair and regeneration, various strategies for skeletal muscle tissue engineering have been developed in the last decades. However, there is still a high demand for the development of new methods and materials that promote skeletal muscle repair and functional regeneration to bring approaches closer to therapies in the clinic that structurally and functionally repair muscle. The combination of stem cells, biomaterials, and biomolecules is used to induce skeletal muscle regeneration. In this review, we provide an overview of different cell types used to treat skeletal muscle injury, highlight current strategies in biomaterial-based approaches, the importance of topography for the successful creation of functional striated muscle fibers, and discuss novel methods for muscle regeneration and challenges for their future clinical implementation. 相似文献
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Vahid Kheiri Mollaqasem Azadeh Asefnejad Mohammad Reza Nourani Vahabodin Goodarzi Mohammad Reza Kalaee 《应用聚合物科学杂志》2021,138(6):49797
Bone tissue scaffolds should have both desired mechanical stability and cell activities including biocompatibility, cell differentiation, and maturation. Also, suitable mineralization is another key factor for these materials. Hence, in current work, in order to achieve a scaffold with desired mechanical and bioactivity properties, core-shell nanofibers based on the polycaprolactone and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with different concentration of graphene oxide (GO) (0.5, 1, and 1.5 wt%) and calcium phosphate (CP) (1 and 3 wt%) were prepared to utilize as bone scaffold. Microstructure of nanofibers observed by field emission scanning electron microscope (FE-SEM) and results exhibited that the most of nanofibers had 270–500 nm diameter. Attenuated total reflectance Fourier transform infrared spectroscopy and energy dispersive X-ray evaluations verified appearance of GO and CP into the electrospun scaffolds (ES). Transmission electron microscopy analysis endorsed core-shell structure of nanofibers. X-ray diffraction study moreover determination of semicrystalline structure, verified presence of GO and CaPO4 into the nanofibers. Water contact angle demonstrates that, ES2 and ES3 situated in suitable domain of hydrophilicity. Tensile analysis determined that, ES2, ES3, and ES4 had the highest mechanical properties for use as bone scaffold. Cell viability assessment confirmed biocompatibility of scaffold during 7 days. Alkaline phosphatase and alizarin red staining exhibited maturating and differentiating of osteocytes after 21 days seeding on the scaffolds. 相似文献
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Jasmin Whittaker Rajkamal Balu Namita R. Choudhury Naba K. Dutta 《Polymer International》2014,63(9):1545-1557
In recent years, protein‐based elastomeric hydrogels have gained increased research interest in biomedical applications for their remarkable self‐assembly behaviour, tunable 3D porous structure, high resilience (elasticity), fatigue lifetime (durability), water uptake, excellent biocompatibility and biological activity. The proteins and polypeptides can be derived naturally (animal or insect sources) or by recombinant (bacterial expression) routes and can be crosslinked via physical or chemical approaches to obtain elastomeric hydrogels. Here we review and present the recent accomplishments in the synthesis, fabrication and biomedical applications of protein‐based elastomeric hydrogels such as elastin, resilin, flagelliform spider silk and their derivatives. © 2013 Society of Chemical Industry 相似文献
15.
Anuj Kumar 《国际聚合物材料杂志》2017,66(4):159-182
Poly (vinyl alcohol) (PVA) is a hydrophilic polymer with excellent biocompatibility and has been applied in various biomedical areas due to its favorable properties. PVA-based hydrogels have been recognized as promising biomaterials and suitable candidates for tissue engineering applications and can be manipulated to act various critical roles. However, due to some disadvantages (i.e., lack of cell-adhesive property), they needs further modification for desired and targeted applications. This review highlights recent progress in the design and fabrication of PVA-based hydrogels, including crosslinking and processing techniques. Finally, major challenges and future perspectives in tissue engineering are briefly discussed. 相似文献
16.
Shear-thinning and self-healing hydrogels are being investigated in various biomedical applications including drug delivery, tissue engineering, and 3D bioprinting. Such hydrogels are formed through dynamic and reversible interactions between polymers or polypeptides that allow these shear-thinning and self-healing properties, including physical associations (e.g., hydrogen bonds, guest–host interactions, biorecognition motifs, hydrophobicity, electrostatics, and metal–ligand coordination) and dynamic covalent chemistry (e.g., Schiff base, oxime chemistry, disulfide bonds, and reversible Diels–Alder). Their shear-thinning properties allow for injectability, as the hydrogel exhibits viscous flow under shear, and their self-healing nature allows for stabilization when shear is removed. Hydrogels can be formulated as uniform polymer and polypeptide assemblies, as hydrogel nanocomposites, or in granular hydrogel form. This review focuses on recent advances in shear-thinning and self-healing hydrogels that are promising for biomedical applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48668. 相似文献
17.
Chau Le Bao Helen Waller Alessandra Dellaquila Daniel Peters Jeremy Lakey Frdric Chaubet Teresa Simon-Yarza 《International journal of molecular sciences》2022,23(23)
In tissue engineering, the composition and the structural arrangement of molecular components within the extracellular matrix (ECM) determine the physical and biochemical features of a scaffold, which consequently modulate cell behavior and function. The microenvironment of the ECM plays a fundamental role in regulating angiogenesis. Numerous strategies in tissue engineering have attempted to control the spatial cues mimicking in vivo angiogenesis by using simplified systems. The aim of this study was to develop 3D porous crosslinked hydrogels with different spatial presentation of pro-angiogenic molecules to guide endothelial cell (EC) behavior. Hydrogels with pores and preformed microchannels were made with pharmaceutical-grade pullulan and dextran and functionalized with novel pro-angiogenic protein polymers (Caf1-YIGSR and Caf1-VEGF). Hydrogel functionalization was achieved by electrostatic interactions via incorporation of diethylaminoethyl (DEAE)–dextran. Spatial-controlled coating of hydrogels was realized through a combination of freeze-drying and physical absorption with Caf1 molecules. Cells in functionalized scaffolds survived, adhered, and proliferated over seven days. When incorporated alone, Caf1-YIGSR mainly induced cell adhesion and proliferation, whereas Caf1-VEGF promoted cell migration and sprouting. Most importantly, directed cell migration required the presence of both proteins in the microchannel and in the pores, highlighting the need for an adhesive substrate provided by Caf1-YIGSR for Caf1-VEGF to be effective. This study demonstrates the ability to guide EC behavior through spatial control of pro-angiogenic cues for the study of pro-angiogenic signals in 3D and to develop pro-angiogenic implantable materials. 相似文献
18.
Anuj Kumar So-Yeon Won Ankur Sood So-Yeon Choi Ritu Singhmar Rakesh Bhaskar Vineet Kumar Sun Mi Zo Sung-Soo Han 《International journal of molecular sciences》2022,23(22)
Hydrogel is a three-dimensional (3D) soft and highly hydrophilic, polymeric network that can swell in water and imbibe a high amount of water or biological fluids. Hydrogels have been used widely in various biomedical applications. Hydrogel may provide a fluidic tissue-like 3D microenvironment by maintaining the original network for tissue engineering. However, their low mechanical performances limit their broad applicability in various functional tissues. This property causes substantial challenges in designing and preparing strong hydrogel networks. Therefore, we report the triple-networked hybrid hydrogel network with enhanced mechanical properties by incorporating dual-crosslinking and nanofillers (e.g., montmorillonite (MMT), graphene nanoplatelets (GNPs)). In this study, we prepared hybrid hydrogels composed of polyacrylamide, poly (vinyl alcohol), sodium alginate, MMT, and MMT/GNPs through dynamic crosslinking. The freeze-dried hybrid hydrogels showed good 3D porous architecture. The results exhibited a magnificent porous structure, interconnected pore-network surface morphology, enhanced mechanical properties, and cellular activity of hybrid hydrogels. 相似文献
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Filippo Rossi Xanthippi Chatzistavrou Giuseppe Perale Aldo R. Boccaccini 《应用聚合物科学杂志》2012,123(1):398-408
Hydrogels studied in this investigation, synthesized starting from agarose and Carbomer 974P, were chosen for their potential use in tissue engineering. The strong ability of hydrogels to mimic living tissues should be complemented with optimized degradation time profiles: a critical property for biomaterials but essential for the integration with target tissue. In this study, chosen hydrogels were characterized both from a rheological and a structural point of view before studying the chemistry of their degradation, which was performed by several analysis: infrared bond response [Fourier transform infrared (FT‐IR)], calorimetry [differential scanning Calorimetry (DSC)], and % mass loss. Degradation behaviors of Agar‐Carbomer hydrogels with different degrees of crosslinkers were evaluated monitoring peak shifts and thermal property changes. It was found that the amount of crosslinks heavily affect the time and the magnitude related to the process. The results indicate that the degradation rates of Agar‐Carbomer hydrogels can be controlled and tuned to adapt the hydrogel degradation kinetics for different cell housing and drug delivery applications. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 相似文献