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1.
The lipid composition of beef and human pituitary was determined by chromatographic and spectrophotometric methods. Beef pituitary
lipid contained about 25% nonpolar lipids and 75% phospholipids whereas nonpolar lipids made up approximately 60% of the total
in human pituitaries. The main nonpolar (i.e., low polarity) lipids in human pituitary were triglycerides, cholesterol, free
fatty acids and an unidentified component in the triglyceride fraction. Cholesterol was the major nonpolar lipid component
in freshly collected beef anterior and posterior pituitary, but the amount of free fatty acids appeared to increase during
storage. Preliminary investigation of the unknown nonpolar lipid in human pituitaries suggested that it was an unsaturated
hydroxy compound with no carbonyl functions. Thin layer chromatography indicated that it was also present in smaller amounts
in freshly collected beef pituitaries. The main phospholipids of beef anterior, posterior and human pituitary were phosphatidyl
ethanolamine, phosphatidyl choline, phosphatidyl inositol, phosphatidyl serine and sphingomyelin. The fatty acid composition
of total nonpolar lipids, free fatty acids, total phospholipids, phosphatidyl ethanolamine and phosphatidyl choline of beef
anterior and posterior pituitary was determined by gas liquid chromatography. Mixtures of saturated and unsaturated fatty
acids ranging from C12 to C22 were present; the main fatty acids were palmitic, stearic, oleic, linoleic and arachidonic. 相似文献
2.
Analyses of renal medullary lipid droplets from normal,hydronephrotic, and indomethacin treated rabbits 总被引:1,自引:0,他引:1
Lipid droplets isolated from rabbit renal medullary tissue were analyzed and found to be composed of triglyceride and free fatty acids in a ratio of 2.9:1. These triglycerides were unique when compared to triglycerides of other rabbit tissues examined, in that they contained high percentages of octadecanoic acid (stearic acid, 9.8%), 5,8,11,14-eicosatetraenoic acid (arachidonic acid, 6.8%), and 7,10,13,16-docosatetraenoic acid (adrenic acid, 10%). Lipid droplet triglycerides were found to increase during experimental hydronephrosis and after administration of indomethacin, a prostaglandin synthetase and phosphodiesterase inhibitor. From gas liquid chromatography of fatty acid methyl esters of these triglycerides, it was determined that they were enriched further in their percent composition of 9,12-octadecadienoic acid (linoleic acid) and arachidonic acid, a prostaglandin precursor. The inverse relationship between lipid droplets and prostaglandin content in the inner medulla suggested a significant role of lipid droplet triglycerides as storage pools for prostaglandin precursors. 相似文献
3.
Total lipids from whole pig adrenal glands as well as from their mitochondria, microsomes, liposomes, and cell sap were extracted and fractionated first into neutral lipids and phospholipids. The highest percentage of neutral lipids was found in the cell sap, and the lowest in the microsomal fraction. Neutral lipids were subfractionated into cholesteryl esters, free cholesterol, triglycerides, and free fatty acids. Cholesteryl esters were distributed throughout the liposomes. Free fatty acids represented a substantial part of cell sap lipids, but were present also in the mitochondria, microsomes, and liposomes. Fatty acids of all fractions were analyzed by gas liquid chromatography. Free fatty acids and cholesteryl ester fatty acids from all cellular fractions were similar in composition and were characterized by considerable quantities of linoleic and arachidonic acid. Triglycerides were characterized by an increased percentage of palmitic and a low content of arachidonic acid. Phosphatidyl choline, phosphatidyl ethanolamine, diphosphatidyl glycerol, and sphingomyelin plus phosphatidyl inositol were isolated from the lipids by preparative thin layer chromatography, and their fatty acids analyzed by gas liquid chromatography. Phosphatidyl choline and phosphatidyl ethanolamine from mitochondria, microsomes, and cell sap were very similar in respect of their fatty acid composition. Sphingomyelin plus phosphatidyl inositol was characterized by a high content of C22:2omega6. Diphosphatidyl glycerol was present in mitochondria and in the cell sap. 相似文献
4.
Henk F. J. Hendriks Pieter J. A. M. Brekelmans Rien Buytenhek Adriaan Brouwer A. Margreet de Leeuw Dick L. Knook 《Lipids》1987,22(4):266-273
The neutral lipid and phospholipid compositions of purified sinusoidal (fat-storing, endothelial and Kupffer) cells, parenchymal
cells and liver homogenates were determined by thin layer chromatography. In addition, the retinoid content of the same purified
cell populations was determined by high performance liquid chromatography. From each cell type, both a lipid droplet fraction
and a pellet fraction (containing the majority of the remaining cell organelles) were prepared by differential centrifugation.
Electron microscopic analysis showed that lipid droplets isolated from fat-storing cells were larger (up to 8 μm) than those
isolated from parenchymal cells (up to 2.5 μm). Moreover, the parenchymal lipid droplets seemed to be surrounded by a membranous
structure, while the fat-storing lipid droplets seemed not to be. Both fat-storing and parenchymal cells contained high concentrations
of neutral lipids, 57.9 μg and 71.0 μg/106 cells, respectively, while endothelial and Kupffer cells contained only 8.6 μg and 13.8 μg/106 cells of neutral lipids, respectively. Sixty-five percent of fat-storing cell lipid droplet fractions comprised esters of
retinol and cholesterol. This combined ester fraction contained mainly retinyl esters. In addition, considerable quantities
(20%) of triglycerides were present. Parenchymal cell lipid droplet fractions comprised triglycerides (62%) and cholesteryl
esters (up to 30%). The pellet fractions prepared from all four cell types consisted mainly of cholesterol (41–67%) and free
fatt acids (20–28%). The phospholipid content was much higher in parenchymal cells than in the sinusoidal liver cell types.
The relative proportions of the four major phospholipid classes were comparable in all liver cell types analyzed. It is concluded
that parenchymal cell lipid droplets comprised mainly triglycerides and cholesteryl esters, which is in agreement with the
function of parenchymal cells in lipid metabolism. Fat-storing cell lipid droplets consisted of retinyl esters and triglycerides,
which correlates well with their function in retionid storage and metabolism. 相似文献
5.
The lipids of human pancreas with special reference to the presence of fatty acid methyl esters 总被引:2,自引:0,他引:2
Total lipids were extracted from human pancreas with chloroform-methanol, chloroform-methanol following acidification, and
benzene. A similar proportional amount of total lipid was obtained by each procedure. Regardless of the method of extraction
(i.e., whether or not methanol was present), a small proportion (about 1%) of the total lipid was found to consist of fatty
acid methyl esters. Triglycerides constituted the major fraction (about 80%) of the pancreatic lipids; in addition to methyl
esters, the remaining lipids comprised free fatty acids, phospholipids, cholesterol esters, and traces of free cholesterol.
In general, each class of lipid had a similar over-all fatty acid composition with palmitic and oleic acids as predominant
components. The methyl esters had a relatively high content of linolenic acid, and the free fatty acids contained a notably
high proportion of palmitic acid, in each case accompanied by a corresponding decrease in the proportion of oleic acid present. 相似文献
6.
The lipid components of four strains ofAgricus bisporus (Lange) Sing., the cultivated mushroom, were analyzed. Both sporophore and mycelial samples were obtained from beds in normal
production. A method for obtaining mycelium free of compost was developed. Neutral lipids were separated from polar lipids
by silicic acid column chromatography. Each fraction was separated by thin layer chromatography. Fatty acid methyl esters
were analyzed by gas liquid chromatography and mass spectrometry. Sporophore extracts contained free sterol, free fatty acid,
triglycerides, phosphatidyl choline and phosphatidyl ethanolamine. High amounts of linoleic acid were found in both neutral
and polar lipid fractions. Mycelial extracts contained free fatty acids, triglycerides, phosphatidylcholine and phosphatidyl
ethanolamine. No free sterol could be detected. Linoleic acid was also present in large amounts.
Paper 3798 in the Journal Series of The Pennsylvania Agricultural Experiment Station. 相似文献
7.
The lipid class and fatty acid composition profiles of mammary glands of female rats fed a nutritionally adequate diet are compared to those of tumors induced in the mammary glands by intravenous injection of dimethylbenz(a)anthracene of animals fed the same diet. Ca. 95% of the lipids of the mammary glands of the control group of animals consisted of triglycerides; glycolipids and phospholipids were present in only minor amounts. In contrast, the lipids of the mammary tumors contained much lower amounts of neutral lipids and higher concentrations of phospholipids. The glycolipid fraction was a minor component of both tissues but differed greatly in composition. The composition of the phospholipid and neutral lipid fractions, particularly the latter, of the mammary tumors also differed from that of the mammary glands of the control animals. The neutral lipids of the tumor tissues contained elevated levels of free fatty acids and cholesterol and much lower concentrations of triglyceride compared to the mammary gland lipids. Differences also were observed in the fatty acid composition of tumor and mammary gland lipid. The greatest differences occurred in the concentrations of polyunsaturated fatty acids which were generally much higher in the tumor lipids. 相似文献
8.
H. P. Nissen G. Ortel K. Macke H. W. Kreysel 《European Journal of Lipid Science and Technology》1983,85(1):39-42
The Skin-Surface Lipids in Psoriasis vulgaris (Ps) - Cholesterol Esters, Wax Esters, Triglycerides and Fatty Alcohols The composition of waxes and cholesterol esters, triglycerides and alcohols of hair lipids from subjects with psoriasis vulgaris and control group was determined by gas liquid chromatography. The amount of palmitoleic acid was lower in the lipid group of the waxes and cholesterol esters in psoriasis vulgaris. Triglycerides show two different changes in the fatty acid pattern by psoriasis: 1) the composition of shorter chain fatty acids was distinct lower but 2) patients with a heavy psoriasis show an increase of the shorter chain fatty acids especially lauric acid and a decrease of the longer chain fatty acids. The analysis of the alcohols indicates that psoriatic patients possess a higher content of alcohols with 12,13 and 14 carbonatoms than normal subjects. 相似文献
9.
Minimal deviation hepatoma 7288C cells were cultured in a modified Swim's medium supplemented with decreasing levels of serum,
lipid-free serum, lipid-free serum plus fatty acids, and other additives. Cellular and media neutral lipid classes were quantitated,
the fatty acids of triglycerides and sterol esters analyzed, and the carbon number distribution of triglycerides determined.
Cellular triglyceride biosynthesis virtually was inhibited when the medium was supplemented with bovine serum alone. This
inhibition was not observed when the medium was supplemented with fetal calf serum alone or mixtures of fetal calf serum and
bovine serum. Cells cultivated on medium supplemented with lipid-free serum plus palmitic or linoleic acids had much lower
levels of free and esterified cholesterol. The fatty acid composition of cellular triglycerides and cholesterol esters differed
dramatically from the corresponding media lipid classes. Except when linoleic acid was added to the medium, changes in the
media serum and lipid levels had only marginal effects upon the fatty acid composition of cellular triglycerides and cholesterol
esters. These data, in conjunction with earlier data that showed the media neutral lipid levels did not decrease during cell
growth, indicate that these hepatoma cells utilize little or no serum triglycerides and cholesterol esters. Linoleic acid
added to the medium dramatically reduced the level of 18∶1 acids in cellular triglycerides and cholesterol esters. Palmitic
acid added to the medium did not change the fatty acid compositions significantly. Comparison of experimentally determined
and calculated triglyceride carbon number percentages indicated a random distribution of fatty acids in this glyceride. The
fatty acid composition of cellular triglycerides was similar to the composition of the cholesterol esters. The lack of characteristic
and distinguishable compositions of these two classes that occur in most normal tissues suggests a loss of specificity in
the lipid metabolism of this neoplasm at the class level. 相似文献
10.
M. R. Sahasrabudhe 《Journal of the American Oil Chemists' Society》1979,56(2):80-84
Compositions of lipids extracted from a sample of Hinoat oat by seven solvent systems and that extracted with chloroform/methanol (2:1 v/v) from six selected cultivars representing high and low lipid contents are reported. Lipid components (steryl esters, triglycerides, partial glycerides, free fatty acids, glycolipids and phospholipids) were separated by silicic acid column chromatography and thin layer chromatography and quantitated by GLC analysis of fatty acids and phosphorus determinations. Twelve oat cultivars were examined for the fatty acid composition of lipid extracted with n-hexane. Lipids extracted from Hinoat by different solvent systems ranged from 5.6 to 8.8%. Quantitative distribution of lipid components extracted with chloroform/methanol from six cultivars containing 4.6 to 11.6% lipid showed a significant correlation (γ=0.99) between the total lipid and the neutral lipid content. Phospholipid content was similar in all cultivars, but glycolipids showed a two-fold increase in high lipid oats. Triglycerides contained less palmitic and more oleic acid than the glycolipids or phospholipids. Nine glycolipids and 11 phospholipids have been identified, and the polar lipid composition of Hinoat oat is presented. 相似文献
11.
M. A. Ali M. A. Gafur M. S. Rahman G. M. Ahmed 《Journal of the American Oil Chemists' Society》1985,62(3):520-523
The kernels of 10 different mango varieties were extracted. The physico-chemical characteristics and lipid class composition
of fats were studied. The fat content of mango kernels grown under the soil and climatic conditions of Bangladesh varied from
7.1% to 10%, depending on the variety. The total lipid extracts were fractionated into lipid classes by a combination of column
and thin layer chromatography (TLC). The hydrocarbon and sterol esters varied from 0.3% to 0.7%, triglycerides from 55.6%
to 91.5%, partial glycerides from 2.3% to 4% and free sterol from 0.3% to 0.6%. Free fatty acids amounted to 3.0–37% as oleic;
glycolipids were 0.6–1.2% and phospholipids 0.11–0.8%. The fatty acid composition of triglyceride (TG) fractions was analyzed
by gas liquid chromatography (GLC). Palmitic acid varied from 7.9 molar % to 10.0 molar %, stearic from 38.2% to 40.2%, oleic
from 41.1% to 43.8%, linoleic from 6.0% to 7.6%, linolenic from 0.6% to 1.0% and arachidic acid from 1.7% to 2.6%. TLC revealed
the presence of lyso-phosphatidylcholine, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and phosphatidic
acid in the phospholipid fraction. 相似文献
12.
Romain Colas Myriam Moret Hubert Vidal Philippe Moulin Michel Lagarde Catherine Calzada 《Lipids》2010,45(8):723-731
Increased oxidative stress is associated with type-2 diabetes and related cardiovascular diseases, but oxidative modification
of LDL has been partially characterized. Our aim was to compare the lipid and fatty acid composition as well as the redox
status of LDL from diabetic patients and healthy subjects. First, to ensure that isolation of LDL by sequential ultracentrifugation
did not result in lipid modifications, lipid composition and peroxide content were determined in LDL isolated either by ultracentrifugation
or fast-protein liquid chromatography. Both methods resulted in similar concentrations of lipids, fatty acids, hydroxy-octadecadienoic
acid (HODE) and malondialdehyde (MDA). Then, LDLs were isolated by ultracentrifugation from eight type-2 diabetic patients
and eight control subjects. Compared to control LDL, diabetic LDL contained decreased cholesteryl esters and increased triglyceride
concentrations. Ethanolamine plasmalogens decreased by 49%. Proportions of linoleic acid decreased in all lipid classes, while
proportions of arachidonic acid increased in cholesteryl esters. Total HODE concentrations increased by 56%, 12- and 15-hydroxy-eicosatetraenoic
acid by 161 and 86%, respectively, and MDA levels increased by twofold. α-Tocopherol concentrations, expressed relative to
triglycerides, were lower in LDL from patients compared to controls, while γ-tocopherol did not differ. Overall, LDL from
type-2 diabetic patients displayed increased oxidative stress. Determination of hydroxylated fatty acids and ethanolamine
plasmalogen depletion could be especially relevant in diabetes. 相似文献
13.
J. E. Kinsella 《Lipids》1972,7(3):165-170
The microsomes isolated from lactating bovine mammary tissue contained 4.3 mg lipid per milligram nitrogen. Phospholipids
comprised 83% of the lipids. The neutral lipids were composed of triglycerides (20–30%), diglycerides (5–10%), free fatty
acids (15–30%, cholesterol (35–40% and cholesterol esters (10–12%, respectively. Phosphatidylcholine was the predominant phospholipid
component (>50%), and the remainder consisted of phosphatidylethanolamine (21–13%), phosphatidylserine (4–6%), phosphatidylinositol
(8%), sphingomyelin (9%) and lysophosphatidylcholine (2%) respectively. The composition of the microsomal phospholipids was
similar to that of isolated mammary cells and tissue homogenates but quite different from milk and fat globule membrane phospholipids.
The triglycerides contained short chain fatty acids but their relative concentrations were lower than in milk triglycerides.
The various lipid fractions had a variable proportion of saturated fatty acids, i.e., triglycerides (47.7%), diglycerides
(86.7%), free fatty acids (70.6%), phosphatidylcholine (50.6%), phosphatidylethanolamine (50.8%), phosphatidylserine (35.3%),
phosphatidylinositol (40.5%) and sphingomyelin (82.3%), respectively. The molecular distribution of fatty acids in the microsomal
triglycerides and phosphatidylcholine was similar to that occurring in milk, i.e., the short chain and unsaturated fatty acids
were concentrated in the primary positions (sn1 andsn3) of the triglycerides, and the unsaturated acids were preferentially located in positionsn2 of the phosphatidylcholine. The compositional data indicate that mammary microsomes are not the direct source of the phospholipids
of the milk fat globule. 相似文献
14.
Groups of normal and hepatoma (7288CTC) bearing rats were maintained on normal chow and fat-free diets for 4 weeks. Normal liver, host liver, and hepatoma neutral lipids were examined in detail and compared. Water content, unaffected by diet was: hepatoma, 82 percent; host liver, 71 percent; and normal liver, 67 percent. The fat-free diet had no effect upon the hepatoma neutral lipids but elevated the triglyceride level in normal and host liver, shifted the triglyceride carbon number distribution to lower mol wt, and elevated the percentage of monoenoic acids in triglycerides and cholesteryl esters. Host triglyceride concentrations were ca. half, and cholesterol levels were reduced moderately relative to normal liver values. Hepatoma cholesterol levels were higher and triglyceride concentrations lower than normal and host liver values. Hepatoma triglycerides differed dramatically from liver and were characterized by increased concentrations of high mol wt species and a fivefold increase in the percentage of C-20 and C-22 fatty acids. The percentage of C-20 and C-22 fatty acids in hepatoma cholesteryl esters also increased ca. fivefold relative to liver. The data indicate that the systems that regulate triglyceride and cholesteryl ester fatty acid composition in liver do not control the compositions of these lipid classes in this hepatoma. The unchanged high level of essential fatty acids in the hepatoma lipids from the fat-free fed animals demonstrates the hepatoma's ability to absorb and conserve specific fatty acids. 相似文献
15.
The influence ofin vivo administration of detergents on serum lipid composition was studied in rats. Male Wistar rats received 50 mg Emulgen 913
(polyoxyethylene nonylphenylether, a nonionic detergent) or SDS (sodium dodecylsulfate, an anionic detergent) per kg of body
weight intraperitoneally for 3 consecutive days. Emulgen 913 and SDS administration increased the level of cholesterol esters
and phospholipids, respectively. But Emulgen 913 administration reduced the level of triglycerides in the Serum, and SDS administration
reduced also the levels of triglycerides and cholesterol esters. In spite of the changes in serum lipid composition, the administration
of these detergents did not affect the amount of total lipids in rat serum. The proportion of palmitic, oleic, and docosahexaenoic
acids in phospholipids was decreased by the administration of Emulgen 913 while the level of arachidonic acid was raised.
However, the level SDS administration had no effect on the fatty acid composition of the serum phospholipids. On the other
hand, both Emulgen 913 and SDS administration showed an effect on the fatty acid composition of triglycerides. It is postulated
that liver damage due to administration of detergents is responsible for the changes in serum lipid and fatty acid composition
in detergent-treated rats. 相似文献
16.
Beef and pork testes, graafian follicles and the residual ovaries were extracted and the lipids from each were separated into
lipid classes by thin-layer chromatography. The fatty acids from each class were analyzed as their methyl esters by gas-liquid
chromatography. The lipids from the reproductive tissues were found to be relatively rich in polyunsaturated acids, many of
which did not correspond to the more commonly encountered unsaturated acids. These less familiar acids were identified by
comparing their chromatographic characteristics with standards of established composition.
The polyunsaturated acids of lipids of the reproductive tissues examined are predominantly of the linoleate family. Only in
the phospholipids of ovarian tissues did the linolenate family of acids reach high proportions of the total polyunsaturates.
Nine members of the linoleate family were identified in the lipids of reproductive tissues. Five higher metabolites of oleate
were identified as normal components of these tissues. Diglycerides were found as a significant lipid class only in testis
tissue. The diglycerides and cholesteryl esters of beef testis contain tetracosatetraenoic acid as major fatty acids. The
triglycerides of reproductive tissues are notably rich in polyunsaturated acids. In the study, 16 polyunsaturated acids were
identified by ozonolysis-reduction and several others were tentatively identified by retention time data. Two acids, previously
unreported, are 10,13,16-docosatrienoic acid and 9,12,15,18-tetracosatetraenoic acid.
Presented in part at the First World Fat Congress, Hamburg, October, 1964, and at the meeting of American Oil Chemists’ Society,
October, 1964. 相似文献
17.
The changes in the lipids of liver tissues of nude mice with and without transplanted human cancerous tissues were studied
to clarify the effect of transplanted human tumor tissues on host liver lipid metabolism. The total lipid was extracted and
separated into phospholipid, triglyceride, and other fractions by thin layer chromatography. The amounts of methyl esters
of fatty acids of each lipid fraction were measured by quantitative gas liquid chromatography after each lipid fraction had
been subjected to methanolysis by 5% HCl-methanol. The phospholipid content of liver tissues of six tumor bearing nude mice
was increased and the triglyceride content decreased in comparison with these fractions in three control nude mice. The ratio
of the phospholipid fatty acid content to the triglyceride fatty acid content (phospholipid∶triglyceride [PL∶TG]) of six tumor
bearing nude mice was distributed between 7.6 and 33.5, whereas PL∶TG ratios of three control nude mice were distributed between
1.7 and 3.8. This result was similar to that reported for human liver tissues of patients with malignant neoplastic disease,
indicating that nude mice with transplanted human cancer may be useful for clarifying the mechanisms of the lipid-chemical
changes of liver tissues of patients with malignancies. 相似文献
18.
L1210 leukemia cells can utilize all of the main fatty acids that normally are present in the ascites fluid in which they
grow. This finding is consistent with the view that L1210 cells derive most of their fatty acids from the ascites fluid. From
80–90% of each fatty acid was incorporated into cell lipids without structural modification, suggesting that the lipid composition
of these cells can be altered by changing the type of fatty acids to which they are exposed. Most importantly, the palmitate
that was subsequently incorporated into total cell phospholipids was elongated and desaturated somewhat more than that incorporated
into triglycerides. This difference was due primarily to more extensive modification of the palmitate incorporated into the
ethanolamine phosphoglycerides fraction. Although there was no difference between total phospholipids and triglycerides with
linoleate, more of the linoleate incorporated into ethanolamine phosphoglycerides was elongated and further desaturated than
that incorporated into choline phosphoglycerides and triglycerides. These findings indicate that fatty acids incorporated
into various cell lipid fractions are not structurally modified to the same extent. There appears to be greater modification
of fatty acid used for ethanolamine phosphoglyceride synthesis as compared with triglyceride and choline phosphoglyceride
synthesis. 相似文献
19.
Jonas Blomberg 《Lipids》1974,9(7):461-470
Pilot whale head oil (blackfish head oil, raw) was analyzed by means of IR spectroscopy, NMR, thin layer chromatography, and
gas liquid chromatography-mass spectrometry. The oil consisted of hydrocarbons (mainly pristane) (3%); waxes and cholesterol
esters (9%); triglycerides (87%) (i.e. non-11%, mono-19% and di-57% isovalero triglycerides) and cholesterol and diglycerides
(1%). By mass spectrometry, the diisovalero triglycerides were shown to be mainly symmetrical. Fatty acids were isobranched
or normal (only traces of anteiso acids were found), saturated, or monounsaturated. Isovaleric acid predominated (54 mole
% fatty acids), the rest having 10–18 carbon atoms. A 5-carbon fatty acid was the only acid found in the waxes. The alcohol
composition qualitatively resembled that of the fatty acids, but major quantitative differences were present. This rules out
direct interconversion of all fatty acids and alcohols. The possible role of these lipids in ultrasound transmission is discussed. 相似文献
20.
Furan fatty acids (F acids) have been found in the livers and/or testes of 20 species, representing 9 families of male freshwater
fish. In 9 species they are major components of the lipids while in the remaining 11 species they occur to a much lesser extent.
The F acids in some species reach a maximum concentration in the testes lipids, and minimum liver lipid concentration, at
spawning. In all species in the testes, the F acids are confined almost exclusively to the triglyceride fraction while, in
the liver lipids, they are found, in order of decreasing concentration, in the cholesteryl esters, the triglycerides, and
the phospholipids. In the lipids of many individuals F6 12,15-epoxy-13,14-dimethyleicosa-12,14-dienoic acid, is the major fatty acid present. It is presumed that these acids perform
some as yet unidentified metabolic function. Isolation technology and identification of F acids by a specific thin layer chromatographic
spray reagent are discussed.
Scientific Journal Series 9910, Agricultural Experiment Station, University of Minnesota, St. Paul, MN 55108. 相似文献