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1.
In this study the FA compositions of healthy and cancerous human renal tissues from the same patients are compared with special
reference to the CLA and PUFA content. CLA was preferentially incorporated into neutral lipid compared with phospholipid classes.
Its distribution profile was similar to that of monounsaturated FA, but unlike that found with 18∶2n−6. Different incorporation
patterns were found for individual CLA isomers. Comparing renal cell carcinoma (RCC) and healthy kidney, the total CLA content
was significantly lower in the cholesterylester fraction and significantly higher in the PE and PS fractions from RCC. The
most significant differences between healthy and cancerous renal tissue were in the content of t10,c12-CLA. Furthermore, the lipid class distributions of n−6 PUFA were determined, and several significant differences between
RCC and healthy renal tissue were found. This is of interest, as it has been proposed that the anticarcinogenic properties
of dietary CLA are associated with their interference in the metabolism of 20∶4n−6. The involvement of CLA in preventing renal
cancer cannot be definitively demonstrated from the design of this study, nor was it intended, but the complete determination
of the FA composition of adjacent healthy and cancerous tissues may provide an insight if lipids are involved in this disease. 相似文献
2.
The fresh-water green alga Parietochloris incisa is the richest plant source of the PUFA arachidonic acid (20∶4n−6, AA). To elucidate the biosynthesis of AA in this alga
we labeled cultures of P. incisa with radioactive precursors. Pulse chase labeling with acetate resulted in its incorporation via the de novo biosynthesis pathway of FA. However, labeled acetate was also utilized for the elongation of C16 and C18 PUFA. Labeling with [1-14C]oleic acid has shown that the first steps of the lipid-linked FA desaturations utilize cytoplasmic lipids. PC and diacylglyceryltrimethylhomoserine
are the major lipids involved as acyl carriers for the Δ12 and Δ6 desaturations of oleic acid, leading sequentially to linoleic
and γ-linolenic acids. The latter is released from its lipid carrier and elongated to 20∶3n−6, which is reincorporated primarily
into PF and PC and finally desaturated to AA. Galactolipids, mostly monogalctosyldiacylglycerol (MGDG), serve as substrates
for the chloroplastic Δ12 desaturase and, apparently, the ω3 desaturation, common to higher plants and many green algae. The
predominant sequence desaturates the 18∶1/16∶0 molecular species of MGDG stepwise to the 18∶3n−3/16∶3n−3 molecular species
similar to the prokaryotic pathway of higher plants and green algae. 相似文献
3.
Milkfat is a complex mixture of many diverse FA, some of which have demonstrated health benefits including anticancer properties.
Attempts are under way to enrich milkfats with long-chain n−3 PUFA and CLA. It has been recommended that the analysis of these
milkfats requires gas chromatography (GC) equipped with long, highly polar capillary columns. However, many analyses have
been reported using CARBOWAXTM type (polyethylene glycol) capillary columns, such as SUPELCOWAX 10, even though the separation characteristics of many of
the FA and their isomers present in milkfats have not been described in detail. This includes the isomers of CLA, cis- and trans-octadecenoic acid (18∶1), linoleic acid (18∶2n−6), and linolenic acid (18∶3n−3), and the long-chain PUFA. On the other hand,
the resolution of these FA and their isomers has been more fully described using the highly polar capillary columns, such
as CP Sil 88 and SP2560 because of the improved resolution obtained using these polar columns. The present study was undertaken
to characterize the separation of these FA present in milkfats using a 60-m SUPELCOWAX 10 column, to compare the results to
those from a 100-m CP Sil 88 column, and to determine if these two columns could possibly serve to complement each other for
the analysis of total milkfat. The advantages of the SUPELCOWAX 10 column were a better resolution of the short-chain saturated
from their monounsaturated FA (MUFA) analogs, and a complete separation of the α-linolenic (18∶3n−3) and eicosadecenoic acid
(20∶1) isomers. It also provided an alternative elution order of the linoleic (18∶2n−6), 18∶3n−3 and γ-linolenic (18∶3n−6)
acid isomers. On the other hand, the CP Sil 88 column provided a better resolution of the CLA isomers, MUFA, the isolated
cis and trans MUFA fractions, the PUFA, and many the 18∶2n−6 and 18∶3n−3 isomers. A complete analysis of milk lipids using the CP Sil 88
column required the prior separation of total FAME using silver ion-TLC. The results of the present study confirm that the
100-m highly polar capillary GC columns are mandatory for the analysis of milk lipids, and at best, the 60 m SUPELCOWAX 10
capillary column serves as a complementary GC column to provide different separations in certain regions based on its intermediate
polarity. 相似文献
4.
The combined effects of age and of diet deficient in n−3 fatty acids on Δ6 desaturation of linoleic acid and on lipid fatty
acid composition were studied in the liver of the rat at 2, 6, 12, 18 and 24 mon of age. The profiles of Δ6 desaturase activity
and fatty acid composition were studied in the deficient rats refed, at these different ages either with 18∶3n−3 (mixture
of peanut and rapeseed oils) or with 20∶5n−3+22∶6n−3 (fish oil) diets for 2, 4, 8 or 12 wk. Results showed that the liver
Δ6 desaturation activity in the control rats remained high at 2 and 6 mon, decreased by 30% from 6 to 12 mon, and then remained
stable from 12 to 24 mon. In the deficient rats, this activity remained high during the entire period studied. Thus, the profile
of liver Δ6 desaturase activity after puberty was not related to age only; it also depended on the polyunsaturated fatty acid
(PUFA) n−6 and n−3 balance in the diet. In the controls, in parallel with the Δ6 desaturase activity, PUFA metabolism could
be divided into three periods: a “young” period, and “old age” period, separated by a period of transition between 6 and 12
mon. Recovery from PUFA n−3 deficiency occurred at all ages but in a different manner depending on whether the rats were “young”
or “old”. Recovery was faster if long-chain n−3 PUFA rather than α-linolenic acid were supplied in the diet. 相似文献
5.
L. Ulmann J. P. Blond C. Maniongui J. P. Poisson G. Durand J. Bézard G. Pascal 《Lipids》1991,26(2):127-133
The combined effects of age and dietary n−6 and n−3 fatty acids were studied in 3-, 6- and 9-month-old rats. At each age,
two groups were fed diets containing 5% (w/w) of vegetable oils rich in either 18∶3n−6 (borage group) or 18∶3n−6 plus 18∶4n−3
(black currant group), for a period increasing with age. A control group was fed the essential fatty acids 18∶2n−6 and 18∶3n−3
only. For each group, Δ6, Δ5 and δ9 desaturase activities were measured in liver microsomes, and fatty acid composition was
determined in microsomal phospholipids. Desaturase activity varied as a function of age and dietary lipids. Δ6 Desaturation
of 18∶3n−3 was more sensitive to these factors while Δ6 desaturation of 18∶2n−6 and Δ9 desaturation were more dependent on
season than the other two. Desaturase activity was influenced more by the black currant than by the borage diet, especially
at 6 and 9 months of age. A large proportion of arachidonic acid was maintained in the microsomes independent of the diet.
Changes in the fatty acid composition did not strictly reflect the differences in desaturase activities. The effects of the
two factors (age and diet) on the activities of the desaturases are complex, suggesting that the enzymes are susceptible to
other factors as well. 相似文献
6.
Marine fish have an absolute dietary requirement for C20 and C22 highly unsaturated fatty acids. Previous studies using cultured cell lines indicated that underlying this requirement in
marine fish was either a deficiency in fatty acyl Δ5 desaturase or C18–20 elongase activity. Recent research in turbot cells found low C18–20 elongase but high Δ5 desaturase activity. In the present study, the fatty acid desaturase/elongase pathway was investigated
in a cell line (SAF-1) from another carnivorous marine fish, sea bream. The metabolic conversions of a range of radiolabeled
polyunsaturated fatty acids that comprised the direct substrates for Δ6 desaturase ([1-14C]18∶2n−6 and [1-14C]18∶3n−3), C18–20 elongase ([U-14C]18∶4n−3), Δ5 desaturase ([1-14C]20∶3n−6 and [1-14C]20∶5n−3), and C20–22 elongase ([1-14C]20∶4n−6 and [1-14C]20∶5n−3) were utilized. The results showed that fatty acyl Δ6 desaturase in SAF-1 cells was highly active and that C18–20 elongase and C20–22 elongase activities were substantial. A deficiency in the desaturation/elongation pathway was clearly identified at the level
of the fatty acyl Δ5 desaturase, which was very low, particularly with 20∶4n−3 as substrate. In comparison, the apparent activities
of Δ6 desaturase, C18–20 elongase, and C20–22 elongase were approximately 94-, 27-, and 16-fold greater than that for Δ5 desaturase toward their respective n−3 polyunsaturated
fatty acid substrates. The evidence obtained in the SAF-1 cell line is consistent with the dietary requirement for C20 and C22 highly unsaturated fatty acids in the marine fish the sea bream, being primarily due to a deficiency in fatty acid Δ5 desaturase
activity. 相似文献
7.
Roberto J. de Antueno Richard C. Cantrill Yung-Sheng Huang Michele Elliot David F. Horrobin 《Lipids》1993,28(4):285-290
This study was undertaken to investigate the total plasma fatty acid composition and the relationship between plasma triacylglycerol
(TG) levels and liver Δ9 desaturase activity in mice fed n−3 and/or n−6 fatty acid or hydrogenated coconut oil (HCO) (maximum
25 mg/g) supplemented diets. Generally, plasma TG levels and Δ9 desaturase activity were inversely correlated with the ratio
of the sum of long chain n−3 fatty acids to 18∶2n−6 and to the ratio of the sum of long chain n−3 fatty acids to 18∶n−3, but
they were positively correlated with the ratio of products and substrates (18∶1/18∶0) of the enzyme in plasma total lipids.
The n−3 fatty acid (mainly 20∶5n−3) enriched diet, when compared to the HCO diet at 21 d, caused a significant reduction in
plasma TG levels but not in Δ9 desaturase activity. However, a marked reduction in plasma TG content (50–60%) and Δ9 desaturase
activity (55–70%) was observed when both 20∶5n−3 and 18∶3n−6 were supplemented in the diet. The plasma TG levels and Δ9 desaturase
activity rose again when the animals were fed the HCO diet or chow. The results suggest that low dose supplementation of a
mixture of n−3 (mainly 20∶5n−3) and n−6 (18∶3n−6) fatty acids modified both plasma TG content and liver Δ9 desaturase activity,
in parallel. 相似文献
8.
Jean François Pageaux Catherine Joulain Jean Michel Fayard Michel Lagarde Christian Laugier 《Lipids》1992,27(7):518-525
The fatty acid (FA) compositions of plasma and oviduct phospholipids (PL) and triglycerides (TG) were studied throughout the
natural sexual development of the Japanese quail. In the oviduct, PL concentration increased rapidly during the period of
active oviduct cell proliferation and then remained at a constant level during the phase of cellular hypertrophy. Oviduct
and plasma TG concentrations were 2- and 10-fold higher, respectively, in fully developed animals than in immature ones. During
natural sexual maturation of the quail, the FA compositions of PL and TG were markedly modified both in plasma and in oviduct.
These qualitative changes occurred predominantly during the period of intense cellular proliferation of oviduct cells, and
also were observed in immature animals injected with physiological doses of estradiol. In oviduct PL, the proportions of 20∶4n−6
and 22∶4n−6 decreased significantly (from 20 to 10% and 3.5 to 0.7%, respectively) whereas those of 18∶2n−6 increased (from
8.5 to 21%). In contrast, the plasma PL proportions of 20∶4n−6, 22∶4n−6 and 18∶2n−6 were decreased significantly and the percentage
of 18∶1n−9 doubled, suggesting that the oviduct is able to utilize certain plasma FA to a greater extent than others. Changes
in plasma and oviduct lipid composition occurring in the quail during sexual development may be attributed to estradiol, which
stimulates hepatic Δ9 desaturase and inhibits the oviduct Δ6 desaturase. The changes in FA composition observed in oviduct
phospholipids are discussed in relation to eicosanoid production and cellular proliferation. 相似文献
9.
The FA composition of Fasciola hepatica 12 kDA purified native FA-binding protein (nFh12), a candidate vaccine against fascioliasis, is described. The FA chain lengths ranged between 12 and 24 carbons. The
principal FA were 16∶0 18∶1n−9, 18∶0, 20∶4n−6, and 20∶1n−9. The acids 16∶0, 18∶1n−9, and 18∶0 comprised over half the FA that
were bound to the whole FA-binding protein. Small amounts (1.0–2.8%) of isoanteiso methyl-branched FA also were characterized. Forty-one different FA were identified in extracts of the adult flukes, with
the three most abundant FA also being 16∶0, 18∶1n−9, and 18∶0. A similar proportion of saturated vs. unsaturated FA was observed
between the whole extract from F. hepatica and the nFh12 protein. However, the n−3/n−6 ratio of PUFA was significantly different, being 1.2 in the whole extract vs. 9.6 in the
nFh12 protein complex. The nFh12 protein binds more n−5, n−6, and n−7 PUFA and less n−3 and n−9 PUFA than the whole extract. In addition, cholesterol
(56%), sitosterol (36%), and fucosterol (8%) also were bound to the nFh12 protein complex. 相似文献
10.
The American marten (Martes americana) is a boreal forest marten with low body adiposity but high metabolic rate. The study describes the FA composition in white
adipose tissue depots of the species and the influence of food deprivation on them. American marten (n=8) were fasted for 2 d with 7 control animals. Fasting resulted in a 13.4% weight loss, while the relative fat mass was >25%
lower in the fasted animals. The FA composition of the fat depots of the trunk was quite similar to other previously studied
mustelids with 14∶0, 16∶0, 18∶0, 16∶1n−7, 18∶1n−9, and 18∶2n−6 as the most abundant FA. In the extremities, there were higher
proportions of monounsaturated FA (MUFA) and PUFA. Food deprivation decreased the proportions of 16∶0 and 16∶1n−7, while the
proportion of long-chain MUFA increased in the trunk. The mobilization of FA was selective, as 16∶1n−7, 18∶1n−9, and particular
n−3 PUFA were preferentially mobilized. Relative mobilization correlated negatively with the carbon chain length in saturated
FA (SFA) and n−9 MUFA. The Δ9 desaturation of SFA enhanced the mobilization of the corresponding MUFA, but the positional
isomerism of the first double bond did not correlate consistently with relative mobilization in MUFA or PUFA. In the marten,
the FA composition of the extremities was highly resistant to fasting, and the tail tip and the paws contained more long-chain
PUFA to prevent the solidification of lipids and to maintain cell membrane fluidity during cooling. 相似文献
11.
Desaturase activities in rat model of insulin resistance induced by a sucrose-rich diet 总被引:1,自引:2,他引:1
Brenner RR Rimoldi OJ Lombardo YB González MS Bernasconi AM Chicco A Basabe JC 《Lipids》2003,38(7):733-742
A sucrose-rich diet, as compared with a similar starch diet, induces a time-dependent typical noninsulin-dependent diabetes
syndrome characterized by insulin resistance in rats. Within the first 3 wk, there was glucose intolerance associated with
hyperinsulinemia, hypertriglyceridemia, and high plasma FFA. In this study, we examined the effect of the sucrose-rich diet
vs. the starch diet during short-(3 wk) and long-term treatment (6 mon) on hepatic Δ9, Δ6, and Δ5 desaturases. These enzymes
modulate monounsaturated FA and PUFA biosynthesis, respectively. Sucrose feeding (3 wk) caused an initial hyperinsulinemia
that was normalized within 6 mon. In the early period (3 wk), stearoyl-CoA desaturase-1 (SCD-1) mRNA and activity were decreased,
whereas Δ6 desaturase mRNA abundance and Δ6 and Δ5 desaturase activities remained unchanged. After 6 mon of sucrose feeding,
activities of the Δ9, Δ6, and Δ5 desaturases were each increased. The SCD-1 and Δ6 desaturase mRNA were also correspondingly
higher. These increases were consistent with an increase in oleic acid, the 20∶4/18∶2 ratio, and 22∶4n−6 and 22∶5n−6 acids
in liver and muscle lipids. On the other hand, the percentage of 22∶6n−3 acid was decreased. In conclusion, a sucrose-rich
diet after 6 mon induces an increase in rat liver SCD-1 and Δ6 desaturase mRNA and enzymatic activities that are opposite
to the changes reported in insulin-dependent diabetes mellitus. It appears that neither blood insulin levels nor insulin resistance
is a factor affecting the Δ9, Δ6, and Δ5 desaturase changes in mRNA and activity found with the sucrose-rich diet. 相似文献
12.
Isolated hepatocytes from rainbow trout and rat were incubated with14C-labeled linoleic acid, linolenic acid, dihomogammalinolenic acid or eicosapentaenoic acid. The most striking difference
in the desaturase activity was the lower level of Δ5 desaturase in trout than in rat. No Δ4 desaturation of 22∶4(n−6) to 22∶5(n−6)
was observed in either of the two species, while the conversion of 22∶5(n−3) to 22∶6(n−3) was significant in both groups and
highest in rainbow trout. The chain-elongating activity was remarkably similar in the two species, except for the “dead-end”
elongation which was distinctly more important in fish. 相似文献
13.
The purpose of the study was to compare the polyunsaturated fatty acid (PUFA) status in patients with X-linked adrenoleukodystrophy
or adrenomyeloneuropathy (X-ALD/AMN) with that in disorders of peroxisome biogenesis (PB). Total fatty acids and plasmalogens
were quantified in plasma and red cells from 28 patients with X-ALD/AMN, 26 patients with generalized peroxisomal disorders,
and 37 controls. Total fatty acid methyl esters and plasmalogen dimethyl acetals were obtained by direct transmethylation
and separated by capillary column gas chromatography. The results confirm previous findings in that docosahexaenoic acid (DHA,
22∶6n−3) was greatly decreased in both plasma and erythrocytes from patients with PB disorders. When nutritional conditions
were adequate, patients with X-ALD/AMN had normal levels of DHA. A highly significant positive correlation was found between
the levels of DHA and those of plasmalogens in peroxisomal patients. As in other tissues, the parent n−6 fatty acid, linoleic
acid (LA, 18∶2n−6) was significantly increased in red cells from PB patients, whereas arachidonic acid (20∶4n−6) was virtually
within normal limits. In clear contrast to red cells and other tissues, arachidonate was significantly lower in plasma from
PB patients. The decrease in plasma arachidonate and the high tissue levels of LA suggest a defect of Δ6 desaturase and/or
Δ5 desaturase in PB patients. The n−6 fatty acids were normal in X-ALD/AMN patients. The present data show that X-ALD/AMN
patients do not have the profound PUFA alterations that PB patients have, at least in blood. 相似文献
14.
The effects of hypothyroidism and of daily treatment for up to 21 days with thyroxin (T4, 0.5 μg/100 g body weight) on the
fatty acid composition of total lipid, phosphatidylethanolamine, and phosphatidylcholine of rat liver mitochondria were studied.
The fatty acid compositions of hypothyroid and euthyroid (control) rats of similar age were compared. The n−6 and n−3 polyunsaturated
fatty acids (PUFA) were affected differently by the hypothyroid state. The levels of linoleic (18∶2n−6), γ-linolenic (18∶3n−6)
and dihomo-γ-linolenic acids (20∶3n−6) were higher in hypothyroid rats than in controls, while the level of arachidonic acid
(20∶4n−6) was lower, which suggests an impairment of the elongase and desaturase activities. The n−3 polyunsaturated fatty
acids, eicosapentaenoic (EPA, 20∶5n−3) and docosapentaenoic (22∶5n−3) acids, were higher in hypothyroid rats, whereas the
linolenic acid (18∶3n−3) content remained constant. The level of docosahexaenoic acid (DHA, 22∶6n−3) was dramatically decreased
in hypothyroid rats, while the levels of C22 n−6 fatty acids were unchanged. The differences were probably due to the competition between n−3 and n−6 PUFA for desaturases,
elongases and acyltransferases. When hypothyroid rats were treated with thyroxin, the changes induced by hypothyroidism in
the proportions of n−6 fatty acids were rapidly reversed, while the changes in the n−3 fatty acids were only partially reversed.
After 21 days of thyroxin treatments, the DHA content was only half as high in hypothyroid rats than in euthyroid rats. These
results suggest that the conversion of 18∶2n−6 to 20∶4n−6 is suppressed in the hypothyroid state which favors the transformation
of 18∶3n−3 to 20∶5n−3. The marked decrease in DHA content indicates an impairment of the enzymes involved in the DHA metabolism,
possibly the n−3 Δ4 desaturase or the acyltransferases. The increased levels of EPA and 22∶5n−3 is consistent with the inhibition
of the n−3 pathway at the Δ4 desaturase step. Observed modifications in the fatty acid composition may significantly alter
eicosanoid synthesis and membrane functions in hypothyroidism. 相似文献
15.
The effects of dietary stearidonic acid (18∶4n−3) on inflammatory mediator release in whole blood and splenocytes was investigated
in Balb/c mice, and the effects were compared with those of two other n−3 PUFA: α-linolenic acid (18∶3n−3) and EPA (20∶5n−3).
TAG mixtures containing 10% of 18∶4n−3, 18∶3n−3, or 20∶5n−3 as the respective sole n−3 PUFA were enzymatically synthesized.
Diets containing synthesized TAG mixtures were fed to Balb/c mice for 3 wk. The release of prostaglandin E2 (PGE2) and tumor necrosis factor (TNF) were measured in whole blood and splenocytes stimulated with lipopolysaccharide. In whole
blood, the production of INF was suppressed by all dietary n−3 PUFA (18∶3n−3, 18∶4n−3, and 20∶5n−3) as compared with the control
diet, which contained TAG prepared from safflower oil. PGE2 production was not significantly changed. Differences among the n−3 PUFA (18∶3n−3), 18∶4n−3, and 20∶5n−3) were not observed.
In splenocytes, PGE2 production was suppressed by dietary n−3 PUFA, but TNF production was not. GC analysis of plasma and splenocyte FA profiles
showed an increase in the levels of 20∶4n−3, 20∶5n−3, and 22∶6n−3 in mice fed the diet containing 18∶4n−3. 相似文献
16.
Dauglas R. Tocher 《Lipids》1993,28(4):267-272
The origin of docosahexaenoic acid (DHA, 22∶6n−3) that accumulates in turbot brain during development was investigated by
studying the incorporation and metabolismvia the desaturase/elongase pathways of [1-14C]-labelled polyunsaturated fatty acids (PUFA) in primary cultures of brain astrocytic glial cells. There was little specificity
evident in the total incorporation of PUFAs into the turbot astrocytes. However, specificity was apparent in the distribution
of the various PUFAs among the individual lipid classes. In particular, there was very specific incorporation of [14C]arachidonic acid (AA, 20∶4n−6) into phosphatidylinositol balanced by a lower incorporation of this acid into total diradyl
glycerophosphocholines. [14C]-Linolenic acid (LNA, 18∶3n−3) and [14C]eicosapentaenoic acid (EPA, 20∶5n−3) were metabolizedvia the desaturase/elongase pathways to a significantly greater extent than [14C]linoleic acid (18∶2n−6) and [14C]AA. The turbot astrocytes expressed very little Δ5 desaturase activity and only low levels of Δ4 desaturation activity.
Although the percentages were small, approximately 4–5 times as much labelled DHA was produced from [14C]EPA compared with [14C]LNA. However, it was concluded that very little DHA in the turbot brain could result from the metabolism of LNA and EPA
in astrocytic glial cells. 相似文献
17.
Supercritical fluid extracts of New Zealand green-lipped mussels (NZGLM) have been suggested to have therapeutic properties
related to their oil components. The large number of minor FA in NZGLM extract was characterized by a GC-CIMS/MS method that
excels at identification of double-bond positions in FAME. The extract contained five major lipid classes: sterol esters,
TAG, FFA, sterols, and polar lipids. The total FA content of the lipid extract was 0.664 g/mL. Fifty-three unsaturated FA
(UFA) were fully identified, of which 37 were PUFA, and a further 21 UFA were detected for which concentrations were too low
for assignment of double-bond positions. There were 17 saturated FA, with 14∶0, 16∶0, and 18∶0 present in the greatest concentration.
The 10 n−3 PUFA detected included 20∶5n−3 and 22∶6n−3, the two main n−3 FA; n−3 PUFA at low concentrations were 18∶3, 18∶4,
20∶3, 20∶4, 21∶5, 22∶5, 24∶6, and 28∶8. There were 43 UFA from the n−4, n−5, n−6, n−7, n−8, n−9, n−10, n−11 families, with
16∶2n−4, 16∶1n−5, 18∶1n−5, 18∶2n−6, 20∶4n−6, 16∶1n−7, 20∶1n−7, 16∶1n−9, 18∶1n−9, and 20∶1n−9 being the most abundant. In general,
we estimated that FAME concentrations greater than 0.05% (w/w) were sufficient to assign double-bond positions. In total,
91 FA were detected in an extract of the NZGLM, whereas previous studies of fresh flesh from the NZGLM had reported identification
of 42 FA. These data demonstrate a remarkable diversity of NZGLM FA. 相似文献
18.
Muscle tissue from the common two-banded sea bream Diplodus vulgaris L. originating from the Adriatic Sea, Croatia, was analyzed. The FA composition of neutral (TAG) and polar (PE, PC, PI/PS)
lipid classes was determined, as well as the lipid and water contents during winter and summer periods. Both the total lipid
and water contents were higher in the winter period. We identified 16 different FA. The major constituents of the total FA
in both seasons were saturates: palmitic (16∶0) and stearic acids (18∶0); monoenes: oleic (18∶1n−9) and palmitoleic acids
(16∶1n−7); and polyunsaturates: arachidonic acid (20∶4n−6), EPA (20∶5n−3), and DHA (22∶6n−3), but their amounts and ratios
differed significantly between the two seasons and between lipid fractions. The FA composition showed a noticeable pattern
of seasonality that reflected fluctuations mainly in TAG. The diminution of the monounsaturated FA content in the summer was
clearly followed by an increase in PUFA content. Diplodus vulgaris is a good source of natural n−3 PUFA and would therefore be suitable for inclusion in highly unsaturated low-fat diets. 相似文献
19.
Total lipid, phospholipid, and FA composition and distribution of FA between polar lipids (PL) and neutral lipids (NL) were
investigated in the boreal soft coral Gersemia rubiformis from the Bering Sea. The total lipids were mostly hydrocarbons and waxes (33.7%) and PL (33.1%). The content of monoalkyldiacylglycerols
(9.7%) exceeded the content of TAG (6.7%). PC and PE constituted 31.4% and 25.6% of total phospholipids, respectively. Principal
FA were 16∶0, 16∶1n−7, 18∶0, 18∶1n−9, 18∶1n−7, 20∶1n−7, 20∶4n−6, 20∶4n−3, 20∶5n−3 22∶5n−3, 22∶6n−3, 24∶5n−6, and 24∶6n−3.
Most n−6 PUFA (52% of total FA) were associated with the PL fraction; this was especially true for arachidonic and tetracosapentaenoic
acids. The NL were enriched with mono-, di-, trienoic, and n−3 PUFA. The variation in EPA levels in both NL and PL suggests
an origin of this acid from lipids of diatoms consumed by the corals. 相似文献
20.
Uptake and metabolism of saturated (16∶0, 18∶0) and unsaturated [18∶1(n−9), 18∶2(n−6), 18∶3(n−3)] fatty acids by cultured
epimastigotes ofTrypanosoma cruzi were studied. Between 17.5 and 33.5% of the total radioactivity of [1-14C]labeled fatty acids initially added to the culture medium was incorporated into the lipids ofT. cruzi and mostly choline and ethanolamine phospholipids. As demonstrated by argentation thin layer chromatography, gas liquid chromatography
and ozonolysis of the fatty acids synthesized, exogenous palmitic acid was elongated to stearic acid, and the latter was desaturated
to oleic acid and 18∶2 fatty acid. The 18∶2 fatty acid was tentatively identified as linoleic acid with the first bond in
the Δ9 position and the second bond toward the terminal methyl end. Exogenous stearic acid was also desaturated to oleic and
18∶2 fatty acid, while oleic acid was only converted into 18∶2. All of the saturated and unsaturated fatty acids investigated
were also converted to a small extent (2–4%) into polyunsaturated fatty acids. No radioactive aldehyde methyl ester fragments
of less than nine carbon atoms were detected after ozonolysis of any of the fatty acids studied. These results demonstrate
the existence of Δ9 and either Δ12 or Δ15 desaturases, or both, inT. cruzi and suggest that Δ6 desaturase or other desaturases of the animal type are likely absent in cultured forms of this organism. 相似文献