Changes in free fatty acid composition during storage of whole milk powder

Changes in fat matter due to the microbial thermostable lipases are among the most significant deteriorations influencing the shelf life of whole milk powder. As a consequence, free fatty acids are produced, being the short-chain acids mainly responsible for the rancidity flavour. The aim of this work was to evaluate changes in different fatty acid concentrations during the storage of whole milk powder produced in two different seasons. Samples of whole milk powder produced in winter and summer and packed under inert atmosphere were used in the study. The samples were stored at 21°C and 40°C. Samples stored at 21°C were evaluated every 3 months, whereas samples stored at 40°C were evaluated monthly by free fatty acids profile and sensory analysis. Data were processed by principal component analysis and anova . Results showed that changes in the free fatty acids of milk powders were correlated with the season of manufacture. Temperature and storage time had little influence on that profile.     

Changes in fatty acids of frog legs during frozen storage

The changes in the fatty acids of frog leg meat during frozen storage were studied with column and gas-liquid chromatography. The major component of the lipids, the phospholipids constituted 90% of total lipids. 16:0, 18:0, 22:0 and 18:2 polyunsaturated fatty acid were the major fatty acids of the frog legs meat lipids. Short chain fatty acids were noticed to the extent of 10%. During frozen storage the proportion of phospholipids decreased, while that of neutral lipids increased. Since the total lipid content was constant, phospholipases were presumably responsible for this change. Alterations in the fatty acid composition of the neutral lipids during storage were consistent with such a hypothesis.     

Irradiation effect on fatty acid composition and conjugated linoleic acid isomers in frozen lamb meat

The effect of gamma radiation processing on the lipid content, fatty acid composition and conjugated linoleic acid (CLA) profile in frozen lamb meat was investigated. Samples of longissimus thoracis muscle from lambs fed lucerne basal diets either unsupplemented or supplemented with polyunsaturated vegetable oils were irradiated (7 kGy) and analysed. CLA contents in lamb meat did not affect (P > 0.05) the levels of lipid oxidation induced by the irradiation. No significant differences (P > 0.05) were observed for fatty acid composition, related nutritional indexes (n − 6/n − 3 and PUFA/SFA), as well as for total lipid and CLA contents, between non-irradiated (control) and irradiated meat samples. In contrast, meat irradiation affected the relative proportions of total trans, trans and cis/trans CLA isomers (P < 0.001), in addition to the percentage of some minor individual CLA isomers (t11, t13 and t9, t11, with P < 0.05 and P < 0.001, respectively). The percentage of total cis/trans CLA isomers slightly decreased in irradiated samples, while the relative proportion of total trans, trans isomers slightly increased. This observation may be explained by the higher susceptibility to autoxidation of the cis double bond relative to the trans configuration.     

Cholesterol oxide, cholesterol, total lipid and fatty acid contents in processed meat products during storage

The effects of storage time on the formation of cholesterol oxides and on alterations in the fatty acid composition of processed meat products manufactured by Brazilian industries were investigated in this study. Cholesterol oxides and cholesterol were determined by HPLC using photodiode array and refractive index detectors. Samples of jerked beef, Italian-type salami, chicken mortadella and Chester mortadella were analysed at 30 day intervals starting at zero time, for 90 days for the mortadella and 120 days for the jerked beef and salami. The mortadellas were stored under refrigeration at 6 °C and the jerked beef and salami at room temperature, but protected from the light. No cholesterol oxides were formed during the storage time in any of the samples. The cholesterol content, the fatty acid composition and total lipid contents showed no significant differences during storage with the exception of the total lipid content of the jerked beef, which varied from 3.5 at zero time to 2.4 g/100 g after 120 days storage.     

Changes of fatty acid composition during the processing of fish

Since the 70's a special attention has been paid to fish oil for its beneficial nutritive effects [1]. Fish products are an important source of polyunsaturated fatty acids (PUFAs) especially n-3 (ω-3) fatty acids - eicosapentaenoic (20:5, n-3) and docosahexaenoic (22:6, n-3) acid which are believed to cause the lowering of the incidence of thrombosis and atherosclerosis [2]. Due to the chemical properties all PUFAs undergo oxidative changes yielding products without any positive nutrition effects. Therefore, the processed fish - preserved, marinated or smoked - differ in the n-3 PUFAs content from the fresh fish meat. The present work aims to compare the effect of smoking and marinating on the n-3 fatty acids content of mackerel meat.     

Wine industry residues extracts as natural antioxidants in raw and cooked chicken meat during frozen storage

The effect of Isabel (IGE) and Niagara (NGE) grape seed and peel extracts on lipid oxidation, instrumental colour, pH and sensory properties of raw and cooked processed chicken meat stored at -18°C for nine months was evaluated. The pH of raw and cooked samples was not affected by the addition of grape extracts. IGE and NGE were effective in inhibiting the lipid oxidation of raw and cooked chicken meat, with results comparable to synthetic antioxidants. The extracts caused alterations in colour, as evidenced by the instrumental (darkening and lower intensity of red and yellow colour) and sensory results of cooked samples. In the sensory evaluation of odour and flavour, IGE produced satisfactory results, which did not differ from synthetic antioxidants. These findings suggest that the IGE and NGE are effective in retarding lipid oxidation of raw and cooked chicken meat during frozen storage.     

Changes in triglyceride fatty acid composition of mammary secretions during involution

Changes in fatty acid composition were determined for fat triglycerides from mammary secretions of 5 Holstein cows during the first 31 d of the nonlactating period. Proportions of short-chain (4:0 to 8:0) and medium-chain (10:0 to 14:0) fatty acids declined by about 50% during the first 3 d of involution, whereas proportions of stearic (18:0) and oleic acids (18:1) increased during the same period. Little change in proportions of those fatty acids occurred after d 3 of involution. Palmitic acid (16:0) was unchanged in proportion during involution. Fatty acid composition of mammary secretion triglycerides changed rapidly in the early nonlactating period, which may reflect a specific decline in de novo fatty acid synthesis in the involuting gland.     

不同冻藏温度对鸡胸肉电阻抗特性的影响

为研究不同冻藏温度条件下鸡胸肉的阻抗特性差异,将白羽鸡鸡胸肉在-10、-20与-30℃的条件下分别冻藏3、6、9、12个月,相应的冻藏时间取20块鸡胸肉解冻,在0.05²00 k Hz的频率范围内,选取6个频率点进行电阻抗特性的分析,检测鸡胸肉的阻抗幅值与阻抗相对变化值（Q值）以及理化品质指标。结果表明:相同冻藏时间内,冻藏温度越高,鸡肉的解冻汁液流失率、脂肪氧化程度、剪切力越大（p<0.05）,蛋白质溶解度越小（p<0.05）。随着检测频率的上升,不同冻藏温度鸡胸肉的阻抗幅值均呈下降趋势。在50 Hz频率时,各个冻藏时间的鸡胸肉在不同冻藏温度条件下的阻抗幅值均存在差异（p<0.05）;在200 k Hz频率时,-10℃处理组的阻抗幅值在冻藏3个月与6个月时显著低于其它处理组（p<0.05）。在冻藏过程中,-10℃冻藏组的Q值均显著高于-30℃处理组的Q值（p<0.05）。冻藏温度对解冻鸡胸肉的阻抗幅值与Q值产生影响,阻抗特性的差异能够反映冷冻鸡胸肉品质的差异,不同冻藏温度鸡肉的电阻抗特性差异为阻抗技术在冻肉品质检测中的应用提供了基础。      

白牦牛肉脂肪酸组成及分析

为分析白牦牛肉的脂肪酸组成及功能特性,采用气相色谱-质谱联用仪(GC-MS)对甘肃天祝白牦牛肉进行定性定量测定,并与当地黄牛肉进行比较。结果表明:白牦牛肉与黄牛肉中的脂肪酸均以饱和脂肪酸为主,在单不饱和脂肪酸和多不饱和脂肪酸尤其是功能性脂肪酸方面,两者有显著的差异(P<0.05或P<0.01),牦牛肉脂肪酸种类更丰富、营养价值更高。     

Effect of dietary fish oil on fatty acid composition, lipid oxidation and sensory property of chicken frankfurters during storage

Broilers fed with three levels of fish oil (0, 2 and 4%) for 6 weeks were used in the manufacture of chicken frankfurters. The meat samples were vacuum-packed and stored at 0°C for 0, 10, 20 and 30 days. Cooking yield, proximate and fatty acid composition, lipid oxidation, microbial and sensory properties of the meat products were measured. Cooking yield, moisture, fat, protein, ash and cholesterol contents of frankfurters were not affected (P>0.05) by levels of dietary fish oil. Frankfurters processed from chickens fed diets enriched with 2 and 4% of supplemental fish oil had a higher (P<0.05) level of n-3 fatty acids (eicosapentaenoic acid and docosahexaenoic acid), but had a lower level of n-6 fatty acids (P<0.05) than the controls (0% fish oil). TBA values and pH values of meat samples were not affected (P>0.05) by levels of supplemental fish oil in the diets, however, pH values of the vacuum-packaged chicken frankfurters decreased (P<0.05) as the storage time increased. No differences (P>0.05) in total anaerobic plate counts were observed among the fish oil treatments during storage. Supplementation of 2 and 4% fish oil in the diets did not increase (P>0.05) the intensity of fishy flavor in chicken frankfurters.     

Cholesterol content and fatty acid composition of rhea meat

The influence of species and muscle on the intramuscular fat content, cholesterol content and fatty acid composition of Greater and Lesser Rhea meat were evaluated. Muscle has no influence (P>0.05) on any of these characteristics. Neither intramuscular fat (1.23%) nor cholesterol content (57 mg/100 g) differed (P>0.05) between species. Despite differences (P<0.05) in the percentage of individual fatty acids, total saturated fatty acids (33.1%) did not differ (P>0.05) between species, while total monounsaturated fatty acids (26.8 vs 32.2%) was lower (P<0.05) and total polyunsaturated fatty acids (39.7 vs 33.6%) higher (P<0.05) for Greater than Lesser Rhea.     

Changes in the chemical composition of rabbit meat during growth

Relative growth rates of water, fat and protein by means of the allometric equation and changes in chemical composition of total meat, meat from hind leg and M. longissimus lumbaris from 1 to 20 weeks of age were studied in 320 rabbits of two strains (New Zealand White and California) and both sexes. All the chemical components showed changes in their allometric coefficients which determined variations in their relative percentages (P < 0·001) during the experimental period. Protein was characterized by a positive allometry although its rate of deposition decreased from 3-4 weeks of age. Water exhibited an important decrease in rate of deposition, passing from positive to negative allometry while, almost simultaneously, the rate of deposition of fat increased, passing from negative to positive allometry; nevertheless, the moment of change of these two components differed among the parts chemically analysed. All these changes were concentrated in a short interval of ages (3-7 weeks of age) that coincided with the one found for anatomic components of the carcass in a previous work (Deltoro & López, 1985). Significant differences (P < 0·001) for mean values were obtained among parts chemically analysed and, to a lesser degree, between lines and sexes. Meat from hind leg and M. longissimus lumbaris had a higher content of protein and a lower content of fat than the rest of the carcass meat. Relating the results to fat-free matter decreased differences but remained significant in most cases and indications of having attained chemical maturity at the end of the experimental period were obtained.     

Measuring changes in radio-frequency dielectric properties of chicken meat during storage

Changes in dielectric properties of stored chicken meat were tracked by using a radio-frequency dielectric spectroscopy method. For this purpose, the dielectric properties were measured with an open-ended coaxial-line probe and a vector network analyzer over a broad frequency range from 200 MHz to 20 GHz at 23 °C. Chicken meat samples were stored in a refrigerator for 8 days at 4 °C. Dielectric constant and loss factor were measured daily over that period. The dielectric constant decreased with frequency and storage duration with a slope change at about 4 GHz. The dielectric loss factor increased with storage duration for frequencies below 3 GHz and remained almost constant above that. The frequency dependence of the dielectric loss factor was dominated by ionic conduction in the lower frequency range and dipolar relaxation of water above 3 GHz. For frequencies below 3 GHz, both the dielectric loss factor and loss tangent increased linearly with storage duration. A three-dimensional representation of the dielectric loss factor as a function of storage duration and conductivity provided analytical expressions that can be used to track chicken meat aging from measurement of the dielectric properties at a single frequency.     

风干牦牛肉氨基酸与脂肪酸组成分析评价

通过对风干牦牛肉原料及成品中氨基酸与脂肪酸的含量进行测定,分析其组成变化,并对风干牦牛肉的营养价值进行评价。结果显示:原料肉和风干牦牛肉均含有十八种氨基酸和十四种脂肪酸,且风干牦牛肉的氨基酸和脂肪酸总量分别高于原料肉1.34g/100g干物质和0.62%（p<0.05）。风干牦牛肉的氨基酸总量为81.14g/100g干物质,EAA/TAA为39.45%,EAA/NEAA为65.15%。脂肪酸总量为97.97%,P:S为0.32,接近理想模式,n-6:n-3为1.4,处于理想范围。因此可以判断风干牦牛肉是一种具有独特风味的优质蛋白质来源,也是具有均衡脂肪酸的高营养价值的肉制品。      

Effect of refrigerated and frozen storage on the survival of Campylobacter jejuni in cooked chicken meat breast

This experimental work aimed to examine the survivability of Campylobacter jejuni in cooked chicken breast under several conditions: storage for 1, 3, and 7 d at refrigerated temperatures (4 °C) and for 20 d at frozen temperatures (-18 °C). In addition, storage at ambient temperature (26 to 28 °C) was involved. Chicken samples were inoculated with a mixed culture of C. jejuni strains (ATCC: 29428 and 33219) of known concentrations (50 and 500 CFU/g). Bacterial cells were recovered and enumerated using standard procedure (Preston method). Bacteria were not detected in the majority of samples stored at ambient temperature. Refrigeration reduced survivals in 95, 90, and 77.5% for samples inoculated with 500 CFU/g and kept for 1, 3, and 7 d, respectively. The maximum reduction reached 1 log(10) cycle for all refrigeration durations. It was observed that bacteria died in 17.5% of samples kept for 7 d at 4 °C. However, survivors in samples inoculated with 50 CFU/g were not detected in 50, 65, and 55% of samples kept for 1, 3, and 7 d, respectively. Freezing rendered survivors not detectable in 70% of samples inoculated with 50 CFU/g, while survived viable counts were reduced in 92.5% of samples inoculated with 500 CFU/g. These findings suggested that C. jejuni could be killed or just sublethally injured with or without reduction in viable counts under the investigated storage temperatures, which may indicate the ability of this bacterium to survive in chicken meat stored under refrigerated and frozen conditions.     

Resistance to freezing and frozen storage of Streptococcus thermophilus is related to membrane fatty acid composition.

The resistance to freezing and frozen storage of Streptococcus thermophilus was related to the fatty acid composition of the cell membrane. The effects of four experimental factors were investigated on the fatty acid concentrations and on the recovery of acidification activity of S. thermophilus stored at -20 degrees C by using a complete experimental design: incorporating oleic acid in the culture medium, fermentation pH, addition of glycerol as cryoprotective agent and duration of storage. The acidification activity decreased during the freezing and the frozen storage of S. thermophilus. The storage time slightly enhanced the unsaturated fatty acid concentrations. The addition of glycerol did not modify the fatty acid composition but increased the resistance to frozen storage. The addition of oleic acid and the decrease of the fermentation pH enhanced the ratio unsaturated:saturated fatty acids and improved the recovery of the acidification activity. These results indicate that the resistance to frozen storage was closely related to the membrane fatty acid composition. We interpreted this as an adaptation of S. thermophilus to the addition of oleic acid and the unfavorable growth conditions that corresponded to a low fermentation pH.     

草鱼脆化过程中腹内脂肪酸组成变化

以不同脆化期的草鱼腹内脂肪为原料,采用气相-色谱质谱（GC-MS）联用技术,研究草鱼脆化过程中腹内脂肪酸组成变化。结果显示,不同脆化期的草鱼腹内脂肪中共检出19种脂肪酸,其中饱和脂肪酸（saturated fatty acid,SFA）7种,单不饱和脂肪酸（monounsaturated fatty acid,MUFA）2种,多不饱和脂肪酸（polyunsaturated fatty acid,PUFA）10种。随着脆化时间的延长,MUFA含量显著增加,PUFA、n-6 PUFA含量显著减少（p<0.05）。整个脆化过程中,SFA∶MUFA∶PUFA的比值近似于1∶2∶1,以普通草鱼最为接近。而n-3 PUFA在脆化20 d时开始减少（p>0.05）,始终低于普通草鱼。n-6/n-3 PUFA的比值为3.36%±0.04%⁴.10%±0.04%,脆化20 d时最高。研究表明,虽然脆化改变了腹内脂肪酸的组成,但脆肉鲩腹内脂肪仍具有较好的开发价值。