Tyr/MWNTs-Chit/GCE生物传感器的制备及其在快速检测大肠杆菌中的应用

该文基于多壁碳纳米管(muhiwalled carbon nanotubes,MWNTs)-壳聚糖(Chit)复合物修饰电极制备了灵敏、稳定的酪氨酸酶(tyrosinase.Tyr)生物传感器.由于MWNTs-Chit复合物具有好的生物兼容性以及电催化能力,Tyrr/MWNTs-Chit/GCE生物传感器在苯酚的检测中具有高灵敏度(412 mA/mo1),低检测限(5.0nmol/L),较宽的线性范围(1.0×10-8～2.8×10-5mol/L)以及良好的稳定性(10天后仍保持93%的活性).把Tyr/MWNTs-Chit/GCE生物传感器进一步应用于大肠杆菌的检测,大肠杆菌在104～107 cfu/mL范围内与电流响应成正比;经过5.0 h的培养,进一步降低大肠杆菌的检测限至10 cfu/mL.     

基于DNA-Hb修饰的聚合二氧化锆/金电极过氧化氢生物传感器的研究

以聚合二氧化锆(ZrO2)薄膜修饰的金电极为基底,通过在二氧化锆修饰电极表面滴涂DNA和血红蛋白(Hb)溶液制备了性能优良的DNA-Hb/ZrO2/Au过氧化氢传感器.该传感器对H2O2的还原显示出较好的电催化响应,固定在电极表面的Hb在0.1 mol/L(pH5.0)PBS中对过氧化氢响应灵敏度高,检测范围宽(1.7×10-7～3.0×10-3～mol/L),检测下限低(8.0×10-8～tool/L),并且表现出良好的热稳定性和高选择性.     

多糖修饰电流型测酚生物电极的响应特性

研制多糖修饰电流型测酚酪氨酸酶碳糊电极.选择了麦芽糊精作为酪氨酸酶的激活剂,采用Nafion 117膜来抗阴离子干扰,聚乙烯亚胺(PEI)作包埋剂以抗阳离子干扰,论文通过正交实验确定了以碳糊电极为基底电极的酪氨酸酶电极的各组分含量为:酪氨酸酶400 U,10%麦芽糊精溶液10μL,0.2%PEI溶液10μL,0.5%Nafion 117溶液2μL.所研制生物电极的工作条件为:工作电位-100mV vs.Hg/Hg2Cl2(s)电极,工作pH为5.40,测量时间1.0 min.在此测量条件下,所研制电极对苯酚的检测下限为5.00×10-8mol/L,线性区间为2.00×10-7mol/L～5.00×10-5mol/L,RSD=1.18%,RE=-0.60%.用所研制电极测定炼油废水中酚的含量测定结果令人满意,加标回收率为100.7%.     

壳聚糖修饰的过氧化氢生物传感器的研究

用壳聚糖固定辣根过氧化物酶(HRP),采用硫堇作为电子媒介,戊二醛作为交联剂,制备了HRP/壳聚糖/硫堇/GC电极.讨论了硫堇的固定方法,考察了该传感器在优化的实验条件下对H2O2的响应特性,实验结果表明,该传感器对H2O2表现出良好的响应特性,如响应快、稳定性好和抗干扰性能强等优点,检测范围为5.0 ×10-6～3.0×10-3mol/L.检出限为1.0×10-6mol/L.电极在用于实际试样回收率的测定中,结果良好.     

基于纳米银/石墨烯/酪氨酸酶/聚酰胺-胺的双酚A传感器

采用石墨烯纳米材料,并结合酪氨酸酶、聚酰胺-胺(PAMAM)和纳米银修饰玻碳电极研制了新型BPA生物传感器。运用循环伏安法和电化学交流阻抗考察了修饰电极的电化学行为。由于石墨烯独特的物理化学性质,结合聚酰胺-胺和纳米银的协同作用,该修饰电极对于BPA有较好的电流响应。在最佳条件下,该传感器对双酚A的线性检测范围为1.0×10-7～3.3×10-5mol/L,检测限为3.0×10-8 mol/L(信噪比为3),相关系数为0.998。     

以萘酚绿B为介体的葡萄糖生物传感器

该文以聚乙烯醇缩丁醛为固定葡萄糖氧化酶(GOD)的载体,将GOD附在铂丝电极上制得葡萄糖酶电极.文中讨论了溶解性媒介体萘酚绿B的浓度、溶液的pH值和温度对该电极电流响应的影响.这一介体型葡萄糖传感器在优化的实验条件下,对葡萄糖表现出良好的响应特性,如响应快、重现性和稳定性好.传感器线性范围为8.0×10-4mol/L～3.6×10-2mol/L,相关系数为0.9933,检测限为5.0×10-4mol/L.     

纳米银／半胱氨酸修饰葡萄糖生物传感器的研究

为优化电流型生物传感器的性能,采用相转移法结合超声波法制备纳米银溶胶,并用透射电镜对其粒径和形态进行了表征.将纳米银/半胱氨酸与葡萄糖氧化酶(GOD)共同修饰在电极表面制成酶电极.结果表明:制备的纳米银粒子近似球形,平均直径为6.27 nm;酶电极的线性响应范围为5.0×10-5～1.5×10-3 mol/L;检出限为7.14×10-6 mol/L(S/N=3);响应时间小于5 s.4℃保存30 d,该电极仍有83.7%的初始响应电流,重复性和稳定性良好.     

基于电聚合膜／溶胶-凝胶固定酶的葡萄糖传感器的研究

该文以铂电极为基底,电聚合乙酰苯胺作为修饰膜,采用氧化锆溶胶-凝胶法固定酶制备葡萄糖酶传感器.探讨了酶的浓度,溶液的pH值等对电极响应的影响,考察了电极的重现性,干扰及使用寿命.该方法有效的排除了尿酸(UA),抗坏血酸(AA)对测定的干扰.该传感器在葡萄糖浓度8.2×10-6～8.0×10-3mol/L范围内有线性响应,检出限为4.1×10-6mol/L.对葡萄糖有较好的响应特性、稳定性和使用寿命.     

一种新型的苯酚电化学传感器研究

制备了一种L-半胱氨卟啉镍(Ⅱ)(NiL)自组装电化学苯酚传感器(NiL/Au CME).该电极具有良好的电活性.实验表明该电极上,苯酚可被NiL催化氧化,氧化峰电流和苯酚浓度成正比,可用于对苯酚进行测定,测定过程无需再向体系中添加媒介体.该电极对苯酚表现出快速的响应(响应时间＜10 s).传感器对苯酚的测定具有较宽的线性范围(2×10-7～3×10-4 mol/L),检出限为1.0×10-7 mol/L.测定了电极稳定性,将该电极用于地表水中苯酚含量检测,并与标准4-氨基安替比林分光光度法作了对照,结果满意.     

一种新型的检测阿特拉津的酪氨酸酶传感器的研制

采用自组装的方法制备了酪氨酸酶传感器,运用循环伏安法探讨了该传感器的电化学特性,研究了阿特拉津在该修饰电极上的电化学行为.实验结果表明,阿特拉津在该修饰电极上具有良好的电流响应.阿特拉津在8.7×10-7～8.2×10-5 mol/L范围内,电流与浓度有良好的线性关系,线性方程为,Ip=-0.328c(Atr)+4.414,相关系数为0.9934,检出限为5.4×10-7 mol/L(信噪比为3).     

浅谈反映人体测量学来源的人体比例理论

人体测量学并不是现代社会的产物。有着很长发展历史的人体比例理论中就包含了现代人体测量学的基本内容,尽管其还不够系统完整,但至少说明了在艺术创作中诞生的人体比例理论对现代人体测量学的影响。并随着时间的推移而不断的完善发展,为现代人体测量学的系统提出奠定了坚实的基础。     

On the relation of the quality of the system with the configuration of the structure of its control device

By examples of the design in the input-output relations of control devices with various configurations, the dependence on the configuration of the value of the criteria characterizing the system quality is shown. The complexity of solving the problem in the state space is illustrated.     

长江水质预测的建模

分析了在长江水质的评价和预测建模过程中出现的一些问题,利用灰色模型的指数特性,建立预测长江水质的GM（1,1）的改进模型。根据数据的周期波动特性,采用灰色系统理论建立了残差序列周期修正GM（1,1）模型,用复合残差来修正预报数据,提高了数据预报的准确程度和模型的适用性。     

Study of the response of the area of the palm to impact: Relationship between the physical characteristic of the palm impact and the subject perception of the severity of the palm impact

Even though using the hand as a hammer causes injury, the practice continues, and may be increasing, in industry and in jobs that require intensive manual labor. This was an exploratory study aimed at measuring the relationships between the forces of strikes, the relative angles of strikes, and/or the number of strikes (up to 3) with the perception of severity of strikes when using the base of the palm of the hand. Eight subjects struck a metal force plate with the base of the palm of the hand at three levels of force, three levels of angle with respect to the surface of a force plate, and with three different numbers of times in a trial. Subjects were then asked to give a psychophysical rating after each trial. The subjective perception of the severity of each impact and force-time curves were recorded. From each force-time curve, peak force, maximum acceleration of force application, and the area of the force curve from initiation to peak were determined. Sixteen ANOVA models were analyzed.     

JSP实现基于中值排序基数法的BBS树状结构

用JSP实现基于中值排序基数法的BBS树状结构,给出数据结构的设计以及具体的算法实现。     

基于单机的虚拟网络实验平台的实现

为了能够解决学生网络实验的可操作性和实验室安全管理的稳定性之间的矛盾,引入VMware,从而在单机上实现虚拟网络实验平台,提高网络实验教学的课堂效果.     

基于单机的虚拟网络实验平台的实现

为了能够解决学生网络实验的可操作性和实验室安全管理的稳定性之间的矛盾,引入VMware,从而在单机上实现虚拟网络实验平台,提高网络实验教学的课堂效果。     

浅谈紫禁城的屋顶文化

紫禁城是我国古代建筑的精品,显示着古代匠师们在建筑上的卓越成就。其苦心经营的屋顶建构更是彰显了宫殿壮丽的威严和飞动的气势。随着我国城市化建设的加速发展,正确地认识中国传统建筑文化并设计具有中国文化的建筑迫在眉睫。本文将从紫荆城屋顶的造型和色彩两方面探析紫禁城的屋顶文化。     

Reconstruction of the corrective component of the transfer function of the interpolator in the process of calibration of the precision event timer

The peculiarities of the interpolation method based on digital signal processing are considered. It is shown that the transfer function of the interpolator may be represented by the composition of two functional components. A nonstatistical method of reconstruction of the form of the corrective component of the transfer function based on processing of the scatter diagram of the ordered pairs of the experimental and predicted data is investigated. It is shown that such pairs may be obtained in the process of periodic event flow timing with estimations of the time positions of the signal and the predetermined instants of the event occurrence being elements of the ordered pairs. The efficiency of the reconstruction method is illustrated by the experimental results.     

Convergence,the university of the future and the future of the university

 The paper questions the ability of current university systems to respond appropriately to the complex demands of an Information Economy. It argues that new relationships between creative subjects and technology require new thinking about the nature and purpose of universities per se. In particular, attention is drawn to the growing involvement of the private sector in higher education. It is argued that it may not be appropriate to think of the `university of the future' in terms of current public sector and quasi public sector institutions, but rather in terms of an emporium, based on an international trade in educational services, and with the `University' as we now understand it occupying the functions of licensing, quality assurance and cultural custodianship. Accepted: 25 June 2002