Forest carbon densities and uncertainties from Lidar, QuickBird, and field measurements in California

Greenhouse gas inventories and emissions reduction programs require robust methods to quantify carbon sequestration in forests. We compare forest carbon estimates from Light Detection and Ranging (Lidar) data and QuickBird high-resolution satellite images, calibrated and validated by field measurements of individual trees. We conducted the tests at two sites in California: (1) 59 km² of secondary and old-growth coast redwood (Sequoia sempervirens) forest (Garcia-Mailliard area) and (2) 58 km² of old-growth Sierra Nevada forest (North Yuba area). Regression of aboveground live tree carbon density, calculated from field measurements, against Lidar height metrics and against QuickBird-derived tree crown diameter generated equations of carbon density as a function of the remote sensing parameters. Employing Monte Carlo methods, we quantified uncertainties of forest carbon estimates from uncertainties in field measurements, remote sensing accuracy, biomass regression equations, and spatial autocorrelation. Validation of QuickBird crown diameters against field measurements of the same trees showed significant correlation (r = 0.82, P < 0.05). Comparison of stand-level Lidar height metrics with field-derived Lorey's mean height showed significant correlation (Garcia-Mailliard r = 0.94, P < 0.0001; North Yuba R = 0.89, P < 0.0001). Field measurements of five aboveground carbon pools (live trees, dead trees, shrubs, coarse woody debris, and litter) yielded aboveground carbon densities (mean ± standard error without Monte Carlo) as high as 320 ± 35 Mg ha^− 1 (old-growth coast redwood) and 510 ± 120 Mg ha^− 1 (red fir [Abies magnifica] forest), as great or greater than tropical rainforest. Lidar and QuickBird detected aboveground carbon in live trees, 70-97% of the total. Large sample sizes in the Monte Carlo analyses of remote sensing data generated low estimates of uncertainty. Lidar showed lower uncertainty and higher accuracy than QuickBird, due to high correlation of biomass to height and undercounting of trees by the crown detection algorithm. Lidar achieved uncertainties of < 1%, providing estimates of aboveground live tree carbon density (mean ± 95% confidence interval with Monte Carlo) of 82 ± 0.7 Mg ha^− 1 in Garcia-Mailliard and 140 ± 0.9 Mg ha^− 1 in North Yuba. The method that we tested, combining field measurements, Lidar, and Monte Carlo, can produce robust wall-to-wall spatial data on forest carbon.     

Measuring biomass changes due to woody encroachment and deforestation/degradation in a forest-savanna boundary region of central Africa using multi-temporal L-band radar backscatter

Satellite L-band synthetic aperture radar backscatter data from 1996 and 2007 (from JERS-1 and ALOS PALSAR respectively), were used with field data collected in 2007 and a back-calibration method to produce biomass maps of a 15 000 km² forest-savanna ecotone region of central Cameroon. The relationship between the radar backscatter and aboveground biomass (AGB) was strong (r² = 0.86 for ALOS HV to biomass plots, r² = 0.95 relating ALOS-derived biomass for 40 suspected unchanged regions to JERS-1 HH). The root mean square error (RMSE) associated with AGB estimation varied from ~ 25% for AGB < 100 Mg ha^− 1 to ~ 40% for AGB > 100 Mg ha^− 1 for the ALOS HV data. Change detection showed a significant loss of AGB over high biomass forests, due to suspected deforestation and degradation, and significant biomass gains along the forest-savanna boundary, particularly in areas of low population density. Analysis of the errors involved showed that radar data can detect changes in broad AGB class in forest-savanna transition areas with an accuracy > 95%. However, quantitative assessment of changes in AGB in Mg ha^− 1 at a pixel level will require radar images from sensors with similar characteristics collecting data from the same season over multiple years.     

Forest structure and aboveground biomass in the southwestern United States from MODIS and MISR

Red band bidirectional reflectance factor data from the NASA MODerate resolution Imaging Spectroradiometer (MODIS) acquired over the southwestern United States were interpreted through a simple geometric-optical (GO) canopy reflectance model to provide maps of fractional crown cover (dimensionless), mean canopy height (m), and aboveground woody biomass (Mg ha^− 1) on a 250 m grid. Model adjustment was performed after dynamic injection of a background contribution predicted via the kernel weights of a bidirectional reflectance distribution function (BRDF) model. Accuracy was assessed with respect to similar maps obtained with data from the NASA Multiangle Imaging Spectroradiometer (MISR) and to contemporaneous US Forest Service (USFS) maps based partly on Forest Inventory and Analysis (FIA) data. MODIS and MISR retrievals of forest fractional cover and mean height both showed compatibility with the USFS maps, with MODIS mean absolute errors (MAE) of 0.09 and 8.4 m respectively, compared with MISR MAE of 0.10 and 2.2 m, respectively. The respective MAE for aboveground woody biomass was ~ 10 Mg ha^− 1, the same as that from MISR, although the MODIS retrievals showed a much weaker correlation, noting that these statistics do not represent evaluation with respect to ground survey data. Good height retrieval accuracies with respect to averages from high resolution discrete return lidar data and matches between mean crown aspect ratio and mean crown radius maps and known vegetation type distributions both support the contention that the GO model results are not spurious when adjusted against MISR bidirectional reflectance factor data. These results highlight an alternative to empirical methods for the exploitation of moderate resolution remote sensing data in the mapping of woody plant canopies and assessment of woody biomass loss and recovery from disturbance in the southwestern United States and in parts of the world where similar environmental conditions prevail.     

Empirical relationships between AIRSAR backscatter and LiDAR-derived forest biomass, Queensland, Australia

To evaluate the use of multi-frequency, polarimetric Synthetic Aperture Radar (SAR) data for quantifying the above ground biomass (AGB) of open forests and woodlands, NASA JPL AIRSAR (POLSAR) data were acquired over a 37 × 60 km area west of Injune, central Queensland, Australia. From field measurements recorded within 32 50 × 50 m plots, AGB was estimated by applying species-specific allometric equations to stand measurements. AGB was then scaled-up to the larger area using relationships established with Light Detection and Ranging (LiDAR) data acquired over 150 (10 columns, 15 rows) 500 × 150 m cells (or Primary Sampling Units, PSUs) spaced 4 × 4 km apart in the north- and east-west directions. Large-scale (1 : 4000) stereo aerial photographs were also acquired for each PSU to assess species composition. Based on the LiDAR extrapolations, the median AGB for the PSU grid was 82 Mg ha^− 1 (maximum 164 Mg ha^− 1), with the higher levels associated with forests containing a high proportion of Angophora and Callitris species. Empirical relationships between AGB and SAR backscatter confirmed that C-, L- and P-band saturated at different levels and revealed a greater strength in the relationship at higher incidence angles and a larger dynamic range and consistency of relationships at HV polarizations. A higher level of saturation (above ∼50 Mg ha^− 1) was observed at C-band HV compared to that reported for closed forests which was attributable to a link between foliage projected cover (FPC) and AGB. The study concludes that L-band HV backscatter data acquired at incidence angles approaching or exceeding 45° are best suited for estimating the AGB up to the saturation level of ∼80-85 Mg ha^− 1. For regional mapping of biomass below the level of saturation, the use of the Japanese Space Exploration Agency (JAXA) Advanced Land Observing Satellite (ALOS) Phase Arrayed L-band SAR (PALSAR) is advocated.     

Regional aboveground live carbon losses due to drought-induced tree dieback in piñon-juniper ecosystems

Recent large-scale dieback of piñon-juniper (P-J) woodlands and forests across the western US occurred as a result of multi-year drought and subsequent insect and disease outbreaks. P-J vegetation is spatially extensive, thus large-scale mortality events such as the one that has occurred over the past several years could significantly alter regional carbon (C) budgets. Our objective was to use a remote sensing technique coupled with field-based data to estimate changes in aboveground live C stocks across a 4100 km² region of Colorado caused by P-J tree mortality. We hypothesized that dieback would amplify the phenological dynamics of P-J vegetation, and these variations would be related to drought-induced losses of live P-J aboveground biomass (AGB) that are discernible using time-series remote sensing vegetation data. Here, we assess live P-J AGB loss using dry season fractional photosynthetic vegetation cover (PV) derived from multi-year Landsat images. Our results showed a strong linear positive relationship between the maximum decline in PV and field-measured losses of live P-J AGB during the period 2000-05 (r² = 0.64, p = 0.002). These results were then used to map AGB losses throughout the study region. Mean live aboveground C loss (± sd) was 10.0 (± 3.4) Mg C ha^− 1. Total aboveground live P-J C loss was 4.6 Tg C, which was approximately 39 times higher than the concurrent C loss attributed to wildfire and management treatments within or near to the national forests of the study region. Our results suggest that spatially extensive mortality events such as the one observed in P-J woodlands across the western US in the past decade may significantly alter the ecosystem C balance for decades to come. Remote sensing techniques to monitor changes in aboveground C stocks, such as the one developed in our study, may support regional and global C monitoring in the future.     

Estimating forage quantity and quality using aerial hyperspectral imagery for northern mixed-grass prairie

Sustainable rangeland stewardship calls for synoptic estimates of rangeland biomass quantity (kg dry matter ha^− 1) and quality [carbon:nitrogen (C:N) ratio]. These data are needed to support estimates of rangeland crude protein in forage, either by percent (CP_c) or by mass (CP_m). Biomass derived from remote sensing data is often compromised by the presence of both photosynthetically active (PV) and non-photosynthetically active (NPV) vegetation. Here, we explicitly quantify PV and NPV biomass using HyMap hyperspectral imagery. Biomass quality, defined as plant C:N ratio, was also estimated using a previously published algorithm. These independent algorithms for forage quantity and quality (both PV and NPV) were evaluated in two northern mixed-grass prairie ecoregions, one in the Northwestern Glaciated Plains (NGGP) and one in the Northwestern Great Plains (NGP). Total biomass (kg ha^− 1) and C:N ratios were mapped with 18% and 8% relative error, respectively. Outputs from both models were combined to quantify crude protein (kg ha^− 1) on a pasture scale. Results suggest synoptic maps of rangeland vegetation mass (both PV and NPV) and quality may be derived from hyperspectral aerial imagery with greater than 80% accuracy.     

Estimating biomass carbon stocks for a Mediterranean forest in central Spain using LiDAR height and intensity data

Biomass fractions (total aboveground, branches and foliage) were estimated from a small footprint discrete-return LiDAR system in an unmanaged Mediterranean forest in central Spain. Several biomass estimation models based on LiDAR height, intensity or height combined with intensity data were explored. Raw intensity data were normalized to a standard range in order to remove the range dependence of the intensity signal. In general terms, intensity-based models provided more accurate predictions of the biomass fractions. Height models selected were mainly based on a percentile of the height distribution. Intensity models selected included variables that consider the percentage of the intensity accumulated at different height percentiles, which implicitly take into account the height distribution. The general models derived considering all species together were based on height combined with intensity data. These models yielded R² values greater than 0.58 for the different biomass fractions considered and RMSE values of 28.89, 18.28 and 1.51 Mg ha⁻¹ for aboveground, branch and foliage biomass, respectively. Results greatly improved for species-specific models using the main species present in each plot, with R² values greater than 0.85, 0.70 and 0.90 for black pine, Spanish juniper and Holm oak, respectively, and with lower RMSE for the biomass fractions. Reductions in LiDAR point density had only a small effect on the results obtained, except for those models based on a variation of the Canopy Reflection Sum, which was weighted by the mean point density. Based on the species-specific equations derived, Holm oak dominated plots showed the highest average carbon contained by aboveground biomass and branch biomass 44.66 and 31.42 Mg ha^− 1 respectively, while for foliage biomass carbon, Spanish juniper showed the highest average value (3.04 Mg ha^− 1).     

The use of waveform lidar to measure northern temperate mixed conifer and deciduous forest structure in New Hampshire

The direct retrieval of canopy height and the estimation of aboveground biomass are two important measures of forest structure that can be quantified by airborne laser scanning at landscape scales. These and other metrics are central to studies attempting to quantify global carbon cycles and to improve understanding of the spatial variation in forest structure evident within differing biomes. Data acquired using NASA's Laser Vegetation Imaging Sensor (LVIS) over the Bartlett Experimental Forest (BEF) in central New Hampshire (USA) was used to assess the performance of waveform lidar in a northern temperate mixed conifer and deciduous forest.Using coincident plots established for this study, we found strong agreement between field and lidar measurements of height (r² = 0.80, p < 0.000) at the footprint level. Allometric calculations of aboveground biomass (AGBM) and LVIS metrics (AGBM: r² = 0.61, PRESS RMSE = 58.0 Mg ha^− 1, p < 0.000) and quadratic mean stem diameter (QMSD) and LVIS metrics (r² = 0.54, p = 0.002) also showed good agreement at the footprint level. Application of a generalized equation for determining AGBM proposed by Lefsky et al. (2002a) to footprint-level field data from Bartlett resulted in a coefficient of determination of 0.55; RMSE = 64.4 Mg ha^− 1; p = 0.002. This is slightly weaker than the strongest relationship found with the best-fit single term regression model.Relationships between a permanent grid of USDA Forest Service inventory plots and the mean values of aggregated LVIS metrics, however, were not as strong. This discrepancy suggests that validation efforts must be cautious in using pre-existing field data networks as a sole means of calibrating and verifying such remote sensing data. Stratification based on land-use or species composition, however, did provide the means to improve regression relationships at this scale. Regression models established at the footprint level for AGBM and QMSD were applied to LVIS data to generate predicted values for the whole of Bartlett. The accuracy of these models was assessed using varying subsets of the USFS NERS plot data. Coefficient of determinations ranged from fair to strong with aspects of land-use history and species composition influencing both the fit and the level of error seen in the predicted relationships.     

Biomass estimation over a large area based on standwise forest inventory data and ASTER and MODIS satellite data: A possibility to verify carbon inventories

According to the IPCC GPG (Intergovernmental Panel on Climate Change, Good Practice Guidance), remote sensing methods are especially suitable for independent verification of the national LULUCF (Land Use, Land-Use Change, and Forestry) carbon pool estimates, particularly the aboveground biomass. In the present study, we demonstrate the potential of standwise (forest stand is a homogenous forest unit with average size of 1-3 ha) forest inventory data, and ASTER and MODIS satellite data for estimating stand volume (m³ ha^− 1) and aboveground biomass (t ha^− 1) over a large area of boreal forests in southern Finland. The regression models, developed using standwise forest inventory data and standwise averages of moderate spatial resolution ASTER data (15 m × 15 m), were utilized to estimate stand volume for coarse resolution MODIS pixels (250 m × 250 m). The MODIS datasets for three 8-day periods produced slightly different predictions, but the averaged MODIS data produced the most accurate estimates. The inaccuracy in radiometric calibration between the datasets, the effect of gridding and compositing artifacts and phenological variability are the most probable reasons for this variability. Averaging of the several MODIS datasets seems to be one possibility to reduce bias. The estimates obtained were significantly close to the district-level mean values provided by the Finnish National Forest Inventory; the relative RMSE was 9.9%. The use of finer spatial resolution data is an essential step to integrate ground measurements with coarse spatial resolution data. Furthermore, the use of standwise forest inventory data reduces co-registration errors and helps in solving the scaling problem between the datasets. The approach employed here can be used for estimating the stand volume and biomass, and as required independent verification data.     

Impact of spatial variability of tropical forest structure on radar estimation of aboveground biomass

Understanding the spatial variability of tropical forest structure and its impact on the radar estimation of aboveground biomass (AGB) is important to assess the scale and accuracy of mapping AGB with future low frequency radar missions. We used forest inventory plots in old growth, secondary succession, and forest plantations at the La Selva Biological Station in Costa Rica to examine the spatial variability of AGB and its impact on the L-band and P-band polarimetric radar estimation of AGB at multiple spatial scales. Field estimation of AGB was determined from tree size measurements and an allometric equation developed for tropical wet forests. The field data showed very high spatial variability of forest structure with no spatial dependence at a scale above 11 m in old-growth forest. Plot sizes of greater than 0.25 ha reduced the coefficients of variation in AGB to below 20% and yielded a stationary and normal distribution of AGB over the landscape. Radar backscatter measurements at all polarization channels were strongly positively correlated with AGB at three scales of 0.25 ha, 0.5 ha, and 1.0 ha. Among these measurements, PHV and LHV showed strong sensitivity to AGB < 300 Mg ha^− 1 and AGB < 150 Mg ha^− 1 respectively at the 1.0 ha scale. The sensitivity varied across forest types because of differences in the effects of forest canopy and gap structure on radar attenuation and scattering. Spatial variability of structure and speckle noise in radar measurements contributed equally to degrading the sensitivity of the radar measurements to AGB at spatial scales less than 1.0 ha. By using algorithms based on polarized radar backscatter, we estimated AGB with RMSE = 22.6 Mg ha^− 1 for AGB < 300 Mg ha^− 1 at P-band and RMSE = 23.8 Mg ha^− 1 for AGB < 150 Mg ha^− 1 at L-band and with the accuracy optimized at 1-ha scale within 95% confidence interval. By adding the forest height, estimated from the C-band Interferometry data as an independent variable to the algorithm, the AGB estimation improved beyond the backscatter sensitivity by 20% at P-band and 40% at L-band. The results suggested the estimation of AGB can be improved substantially from the fusion of lidar or InSAR derived forest height with the polarimetric backscatter.     

Quantification of aboveground rangeland productivity and anthropogenic degradation on the Arabian Peninsula using Landsat imagery and field inventory data

The productivity of semi-arid rangelands on the Arabian Peninsula is spatially and temporally highly variable, and increasing grazing pressure as well as the likely effects of climatic change further threatens vegetation resources. Using the Al Jabal al Akhdar mountains in northern Oman as an example, our objectives were to analyse the availability and spatial distribution of aboveground net primary production (ANPP) and the extent and causes of vegetation changes during the last decades with a remote sensing approach. A combination of destructive and non-destructive biomass measurements by life-form specific allometric equations was used to identify the ANPP of the ground vegetation (< 50 cm) and the leaf and twig biomass of phanerophytes. The ANPP differed significantly among the life forms and the different plant communities, and the biomass of the sparsely vegetated ground was more than 50 times lower (mean = 0.22 t DM ha^− 1) than the biomass of phanerophytes (mean = 12.3 t DM ha^− 1). Among the different vegetation indices calculated NDVI proved to be the best predictor for rangeland biomass.Temporal trend analysis of Landsat satellite images from 1986 to 2009 was conducted using a pixel-based least square regression with the annual maximum Normalized Differenced Vegetation Index (NDVI_max) as a dependent variable. Additionally, linear relationships of NDVI_max and annual rainfall along the time series were calculated. The extent of human-induced changes was analysed using the residual trends method. A strongly significant negative biomass trend detected for 83% of the study area reflected a decrease in annual rainfall but even without clear evidence of deforestation of trees and shrubs, human-induced vegetation degradation due to settlement activities were also important.     

Model-assisted regional forest biomass estimation using LiDAR and InSAR as auxiliary data: A case study from a boreal forest area

There is a need for accurate inventory methods that produce relevant and timely information on the forest resources and carbon stocks for forest management planning and for implementation of national strategies under the United Nations Collaborative Program on Reduced Emissions from Deforestation and Forest Degradation in Developing Countries (REDD). Such methods should produce information that is consistent across various geographical scales. Airborne scanning Light Detection and Ranging (LiDAR) is among the most promising remote sensing technologies for estimation of forest resource information such as timber volume and biomass, while acquisition of three dimensional data with Interferometric Synthetic Aperture Radar (InSAR) from space is seen as a relevant option for inventory in the tropics because of its ability to “see through the clouds” and its potential for frequent updates at low costs. Based on a stratified probability sample of 201 field survey plots collected in a 960 km² boreal forest area in Norway, we demonstrate how total above-ground biomass (AGB) can be estimated at three distinct geographical levels in such a way that the estimates at a smaller level always sum up to the estimate at a larger level. The three levels are (1) a district (the entire study area), (2) a village, local community or estate level, and (3) a stand or patch level. The LiDAR and InSAR data were treated as auxiliary information in the estimation. At the two largest geographical levels model-assisted estimators were employed. A model-based estimation was conducted at the smallest level. Estimates of AGB and corresponding error estimates based on (1) the field sample survey were compared with estimates obtained by using (2) LiDAR and (3) InSAR data as auxiliary information. For the entire study area, the estimates of AGB were 116.0, 101.2, and 111.3 Mg ha⁻¹, respectively. Corresponding standard error estimates were 3.7, 1.6, and 3.2 Mg ha⁻¹. At the smallest geographical level (stand) an independent validation on 35 large field plots was carried out. RMSE values of 17.1-17.3 Mg ha⁻¹ and 42.6-53.2 Mg ha⁻¹ were found for LiDAR and InSAR, respectively. A time lag of six years between acquisition of InSAR data and field inventory has introduced some errors. Significant differences between estimates and reference values were found, illustrating the risk of using pure model-based methods in the estimation when there is a lack of fit in the models. We conclude that the examined remote sensing techniques can provide biomass estimates with smaller estimated errors than a field-based sample survey. The improvement can be highly significant, especially for LiDAR.     

Mapping biomass and stress in the Sierra Nevada using lidar and hyperspectral data fusion

In this paper, we explored fusion of structural metrics from the Laser Vegetation Imaging Sensor (LVIS) and spectral characteristics from the Airborne Visible Infrared Imaging Spectrometer (AVIRIS) for biomass estimation in the Sierra Nevada. In addition, we combined the two sensors to map species-specific biomass and stress at landscape scale. Multiple endmember spectral mixture analysis (MESMA) was used to classify vegetation from AVIRIS images and obtain sub-pixel fractions of green vegetation, non-photosynthetic vegetation, soil, and shade. LVIS metrics, AVIRIS spectral indices, and MESMA fractions were compared with field measures of biomass using linear and stepwise regressions at stand (1 ha) level. AVIRIS metrics such as water band indices and shade fractions showed strong correlation with LVIS canopy height (r² = 0.69, RMSE = 5.2 m) and explained around 60% variability in biomass. LVIS variables were found to be consistently good predictors of total and species specific biomass (r² = 0.77, RMSE = 70.12 Mg/ha). Prediction by LVIS after species stratification of field data reduced errors by 12% (r² = 0.84, RMSE = 58.78 Mg/ha) over using LVIS metrics alone. Species-specific biomass maps and associated errors created from fusion were different from those produced without fusion, particularly for hardwoods and pines, although mean biomass differences between the two techniques were not statistically significant. A combined analysis of spatial maps from LVIS and AVIRIS showed increased water and chlorophyll stress in several high biomass stands in the study area. This study provides further evidence that lidar is better suited for biomass estimation, per se, while the best use of hyperspectral data may be to refine biomass predictions through a priori species stratification, while also providing information on canopy state, such as stress. Together, the two sensors have many potential applications in carbon dynamics, ecological and habitat studies.     

A LiDAR-derived canopy density model for tree stem and crown mapping in Australian forests

The retrieval of tree and forest structural attributes from Light Detection and Ranging (LiDAR) data has focused largely on utilising canopy height models, but these have proved only partially useful for mapping and attributing stems in complex, multi-layered forests. As a complementary approach, this paper presents a new index, termed the Height-Scaled Crown Openness Index (HSCOI), which provides a quantitative measure of the relative penetration of LiDAR pulses into the canopy. The HSCOI was developed from small footprint discrete return LiDAR data acquired over mixed species woodlands and open forests near Injune, Queensland, Australia, and allowed individual trees to be located (including those in the sub-canopy) and attributed with height using relationships (r² = 0.81, RMSE = 1.85 m, n = 115; 4 outliers removed) established with field data. A threshold contour of the HSCOI surface that encompassed ∼ 90% of LiDAR vegetation returns also facilitated mapping of forest areas, delineation of tree crowns and clusters, and estimation of canopy cover. At a stand level, tree density compared well with field measurements (r² = 0.82, RMSE = 133 stems ha^− 1, n = 30), with the most consistent results observed for stem densities ≤ 700 stems ha^− 1. By combining information extracted from both the HSCOI and the canopy height model, predominant stem height (r² = 0.91, RMSE = 0.77 m, n = 30), crown cover (r² = 0.78, RMSE = 9.25%, n = 30), and Foliage & Branch Projective Cover (FBPC; r² = 0.89, RMSE = 5.49%, n = 30) were estimated to levels sufficient for inventory of woodland and open forest structural types. When the approach was applied to forests in north east Victoria, stem density and crown cover were reliably estimated for forests with a structure similar to those observed in Queensland, but less so for forests of greater height and canopy closure.     

Estimation of diurnal air temperature using MSG SEVIRI data in West Africa

Spatially distributed air temperature data with high temporal resolution are desired for several modeling applications. By exploiting the thermal split window channels in combination with the red and near infrared channels of the geostationary MSG SEVIRI sensor, multiple daily air temperature estimates can be achieved using the contextual temperature-vegetation index method. Air temperature was estimated for 436 image acquisitions during the 2005 rainy season over West Africa and evaluated against in situ data from a field test site in Dahra, Northern Senegal. The methodology was adjusted using data from the test site resulting in RMSE = 2.55 K, MBE = − 0.30 K and R² = 0.63 for the estimated versus observed air temperatures. A spatial validation of the method using 12 synoptic weather stations from Senegal and Mali within the Senegal River basin resulted in overall values of RMSE = 2.96 K, MBE = − 1.11 K and R² = 0.68. The daytime temperature curve is interpolated using a sine function based on the multiple daily air temperature estimates from the SEVIRI data. These estimates (covering the 8:00-20:00 UCT time window) were in good agreement with observed values with RMSE = 2.99 K, MBE = − 0.70 K and R² = 0.64. The temperature-vegetation index method was applied as a moving window technique to produce distributed maps of air temperature with 15 min intervals and 3 km spatial resolution for application in a distributed hydrological model.     

Estimating Siberian timber volume using MODIS and ICESat/GLAS

Geosciences Laser Altimeter System (GLAS) space LiDAR data are used to attribute a MODerate resolution Imaging Spectrometer (MODIS) 500 m land cover classification of a 10° latitude by 12° longitude study area in south-central Siberia. Timber volume estimates are generated for 16 forest classes, i.e., four forest cover types × four canopy density classes, across this 811,414 km² area and compared with a ground-based regional volume estimate. Two regional GLAS/MODIS timber volume products, one considering only those pulses falling on slopes ≤ 10° and one utilizing all GLAS pulses regardless of slope, are generated. Using a two-phase(GLAS-ground plot) sampling design, GLAS/MODIS volumes average 163.4 ± 11.8 m³/ha across all 16 forest classes based on GLAS pulses on slopes ≤ 10° and 171.9 ± 12.4 m³/ha considering GLAS shots on all slopes. The increase in regional GLAS volume per-hectare estimates as a function of increasing slope most likely illustrate the effects of vertical waveform expansion due to the convolution of topography with the forest canopy response. A comparable, independent, ground-based estimate is 146 m³/ha [Shepashenko, D., Shvidenko, A., and Nilsson, S. (1998). Phytomass (live biomass) and carbon of Siberian forests. Biomass and Bioenergy, 14, 21-31], a difference of 11.9% and 17.7% for GLAS shots on slopes ≤ 10° and all GLAS shots regardless of slope, respectively. A ground-based estimate of total volume for the entire study area, 7.46 × 10⁹ m³, is derived using Shepashenko et al.'s per-hectare volume estimate in conjunction with forest area derived from a 1990 forest map [Grasia, M.G. (ed.). (1990). Forest Map of USSR. Soyuzgiproleskhoz, Moscow, RU. Scale: 1:2,500,000]. The comparable GLAS/MODIS estimate is 7.38 × 10⁹ m³, a difference of less than 1.1%. Results indicate that GLAS data can be used to attribute digital land cover maps to estimate forest resources over subcontinental areas encompassing hundreds of thousands of square kilometers.     

An assessment of the black ocean pixel assumption for MODIS SWIR bands

Recent studies show that an atmospheric correction algorithm using shortwave infrared (SWIR) bands improves satellite-derived ocean color products in turbid coastal waters. In this paper, the black pixel assumption (i.e., zero water-leaving radiance contribution) over the ocean for the Moderate Resolution Imaging Spectroradiometer (MODIS) SWIR bands at 1240, 1640, and 2130 nm is assessed for various coastal ocean regions. The black pixel assumption is found to be generally valid with the MODIS SWIR bands at 1640 and 2130 nm even for extremely turbid waters. For the MODIS 1240 nm band, however, ocean radiance contribution is generally negligible in mildly turbid waters such as regions along the U.S. east coast, while some slight radiance contributions are observed in extremely turbid waters, e.g., some regions along the China east coast, the estuary of the La Plata River. Particularly, in the Hangzhou Bay, the ocean radiance contribution at the SWIR band 1240 nm results in an overcorrection of atmospheric and surface effects, leading to errors of MODIS-derived normalized water-leaving radiance at the blue reaching ~ 0.5 mW cm^− 2 μm^− 1 sr^− 1. In addition, we found that, for non-extremely turbid waters, i.e., the ocean contribution at the near-infrared (NIR) band < ~ 1.0 mW cm^− 2 μm^− 1 sr^− 1, there exists a good relationship in the regional normalized water-leaving radiances between the red and the NIR bands. Thus, for non-extremely turbid waters, such a red-NIR radiance relationship derived regionally can possibly be used for making corrections for the regional NIR ocean contributions without using the SWIR bands, e.g., for atmospheric correction of ocean color products derived from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS).     

Direct electrochemistry of cytochrome P450 6A1 in mimic bio-membrane and its application for pesticides sensing

The direct electrochemistry of house fly cytochrome P4506A1 (CYP6A1) confined in dioctadecyl dimethyl ammonium bromide (DDAB) film was achieved. The immobilized CYP6A1 displayed a pair of redox peaks with a formal potential of −0.36 mV in pH 7.0 O₂-free phosphate buffers at scan rate of 1 V s⁻¹ and the direct electron transfer of CYP6A1 was characterized by voltammetry. The CYP6A1 in the DDAB film retained its bioactivity and could catalyze the reduction of dissolved oxygen. Upon addition of its substrate aldrin or heptachlor to the air-saturated solution, the reduction peak current of dissolved oxygen increased, which indicates the catalytic behavior of CYP6A1 to its substrates. By amperometry a calibration linear range was obtained to be 9.08 × 10⁻⁶-4.54 × 10⁻⁵ mol L⁻¹ with a sensitivity of 80 μA mM⁻¹ for aldrin or 8.91 × 10⁻⁶-4.46 × 10⁻⁵ mol L⁻¹ with a sensitivity of 66 μA mM⁻¹ for heptachlor. The apparent Michaelis-Menten constant for the electrocatalytic activity of CYP6A1 was found to be 7.468 × 10⁻⁵ mol L⁻¹ for aldrin and 4.316 × 10⁻⁵ mol L⁻¹ for heptachlor. The bioelectrocatalytic products were analysed using gas chromatography (GC) and electron ionization-mass spectrometry (EI-MS). The results confirmed that epoxidation was the main pathways of CYP6A1-mediated organochlorine pesticides oxidation.     

Response of the Sundarbans coastline to sea level rise and decreased sediment flow: A remote sensing assessment

The Sundarbans is the world's largest remaining single block of mangrove forest, covering approximately 1 million ha (~ 10,000 km²) of the Ganges-Brahmaputra delta along the coastal areas of India and Bangladesh. Sea level rise and alteration of water flows of the Himalayan headwaters are among the major disturbances threatening these coastal areas. But very few studies exist on the dynamics or current status of the Sundarbans coastline. We used Landsat images spanning from 1973 to 2010, and an algorithm that we developed, to consistently estimate the spatiotemporal dynamics of erosion and accretion for four different time intervals and the whole study period. Our results show that the direction and extent of erosion and accretion rates varied throughout the different periods. Erosion was the highest in the 1973-1979 interval, with 23.2 km² year⁻¹ of land loss. However, that rate substantially declined in the following periods, reaching a rate of 7-10 km² year⁻¹. Accretion showed a rate of 10 km² year⁻¹ between 1973 and 1989, but substantially declined to ~ 4 km² year⁻¹ between 1989 and 2010. Accretion rate has declined in the recent years but erosion rate has remained relatively high. As a result the delta front has undergone a net erosion of ~ 170 km² of coastal land in the 37 years of our study period. These numbers are significantly higher than the previously reported rates and magnitudes of erosion in this area. The methods and maps developed in this study may be helpful in management planning of this vulnerable coastline.