分子印迹技术研究进展

分子印迹技术是近些年来快速发展的一种高选择性分离及分子识别技术,由该技术制备的具有分子特异识别功能的高分子材料称为分子印迹聚合物。本文就其基本原理、方法及其在天然产物分离、食品检测、仿生传感、固相萃取、抗体与受体模拟领域的应用与研究进行综述。     

分子印迹模板分子类型研究进展

综述了模板分子的选择概况,包括有机小分子、生物大分子、细胞和病毒、无机离子和虚拟模板分子,并对模板分子的局限性做了详细介绍,以期对分子印迹技术的发展提供一些参考。     

分子识别和分子印迹聚合物微球

分子识别在许多分离、催化以及生物化学过程中常常起着决定性作用。例如 ,分子结构上的微小差别往往可以决定某个生化反应 (如酶反应 )是否能够顺利进行 ,所以长期以来 ,科学家们一直努力寻找分子识别的途径。二十多年前 ,Ｗｕｌｆｆ等采用一种全新的被称作分子印迹(Ｍｏｌｅｃｕｌａｒｉｍｐｒｉｎｔｉｎｇ)的技术合成出了对糖类和氨基酸衍生物有识别作用的聚合物。此聚合物被称为分子印迹聚合物 (亦称分子模板聚合物 )。分子印迹技术就是指以特定的分子为模板 ,制备对该分子有特殊识别功能和高选择性材料的技术。该技术在最近几年内发展极…     

克伦特罗分子印迹聚合物吸附性能研究

选用分子印迹技术,以克伦特罗为印迹分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成克伦特罗分子印迹聚合物,研究其吸附性能。结果表明,与组成相同的非印迹聚合物相比,克伦特罗印迹聚合物有较大的吸附性能,有望在克伦特罗分离分析中作为固相萃取的材料。     

分子印迹技术应用研究

分子印迹技术是在近年来才发展起来的一项新兴技术,具备分子特异识别的功能.本文对其发展历程、基本原理及其在分析化学、天然产物化学、生物学、膜分离、有机合成等方面在国内外应用现状进行综述.     

分子印迹材料的制备与应用

分子印迹技术（molecularly imprinted technique,MIT）来源于免疫学、生物化学,是模拟生物体内抗体与抗原、酶与底物等分子之间的识别过程,是合成对某一种分子具有专一识别性的聚合物的方法。本文对分子印迹材料的合成方法——共价法、非共价法和半共价法等进行了综述,并介绍了分子印迹材料与技术在分析化学与生物领域的应用。     

分子印迹技术研究进展

分子印迹技术作为一种新型的亲和分离技术,是当前发展高选择性材料的主要技术之一。利用该技术所制备的分子印迹聚合物具有分子特异识别功能。本文主要介绍分子印迹技术的方法、基本原理、分子印迹聚合物的制备方法及其在食品分析、环境分析和药物分析中的应用,并对以后的发展方向提出了展望。     

基于分子识别和分子印迹的三氯生新型功能材料设计及性能研究

利用分子印迹技术的特异性识别,采用本体热聚合,以酸碱相互作用为基本作用机理,分别以甲基丙烯酸二甲氨基乙酯(DMAEMA)、甲基丙烯酸二乙氨基乙酯(DEAM)、甲基丙烯酸-2-氨基乙基酯盐酸盐(AMA盐酸盐)、4-乙烯基吡啶(4-VP)和甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)和二乙烯基苯(DVB)为交联剂,偶氮二异丁腈(AIBN)为引发剂,乙腈为溶剂设计合成了三氯生(TCS)的分子印迹聚合物(MIPs)。吸附结果表明,其中DEAM是TCS的最佳功能单体,吸附率达到了78.5%,印迹因子达到了1.7,用DEAM作为功能单体合成的MIPs对TCS的选择性实验结果显示,对TCS的吸附容量明显高于其结构类似物,对TCS的吸附实验结果显示,5次回收后重复利用,吸附容量仅降低了5.1%,表明该MIPs可以重复使用多次。     

阿司匹林分子印迹聚合物的制备及分子识别性能研究

以阿司匹林为模板、丙烯酰胺为功能单体制备了能特异性识别阿司匹林的分子印迹聚合物,研究结果表明,该分子印迹聚合物通过特异的氢键作用力对阿司匹林进行识别,静态分布系数为5 65,对阿司匹林与结构类似物如苯、苯甲酸、乙酸苯酯、水杨酸的分离因子均大于1 89,选择性高。该分子印迹聚合物对阿司匹林的最大表观吸附量Qmax为67 7mg/g,平衡离解常数Kd为2 33×10-4mol/L,对阿司匹林的选择性吸附可在30min内基本完成。     

三氯卡班分子印迹聚合物的设计、合成及性能研究

以氢键相互作用为基本工作机理,采用本体热聚合方法,以含脲基化合物FM1、FM2和FM3为功能单体,DVB为交联剂,AIBN为引发剂,四氢呋喃为溶剂,合成了三氯卡班的分子印迹聚合物(MIPs)。研究表明,MIPs的最佳功能单体是FM3,MIPs对TCC的最高吸附率达到了93. 9%,印迹因子达到了2. 0,可多次重复利用。     

分子印迹技术在甾类物质识别和分析中的应用

分子印迹技术在甾类分子识别和分析中的应用及取得的进展。     

分子印迹技术及其在烟草中的应用研究进展

分子印迹技术具有构效预定性、特异识别性和广泛实用性的特点。介绍了分子印迹技术的产生、原理,综述了其在烟草中的应用。对其在烟草分析中的应用、农药残留快速分析和在卷烟行业减害降焦方面的研究进行了总结,并对此项新技术在烟草行业中的机理研究、新型功能单体的制备和在线监测技术等方面的应用进行了展望。     

Template imprinting amphoteric polymer for the recognition of proteins

A selective recognizing of proteins with amphoteric polymer is essential for application in protein separations. An amphoteric polymer was synthesized by copolymerization of the two functional monomers with the crosslinker in the presence of the template proteins. After extraction of the template protein, the polymer was applied as chromatographic stationary phase, showing high resolution capacity. The molecular imprinting technique allows the formation of specific recognition sites in amphoteric polymers through the use of template protein. Protein is preferentially bound to a polymer made in the presence of the same protein. The recognition ability of an amphoteric polymer is attributed to imprinted cavities that complement proteins in shape and electrostatic interactions. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 95: 358–361, 2005     

智能分子印迹水凝胶的研究进展

分子印迹技术具有预定性、识别性和实用性的特点,而智能水凝胶在外界环境刺激下能产生体积或相的转变。主要介绍了将2者结合起来的一类智能分子印迹水凝胶。它是一种能够实现环境调变响应的目标分子吸收与释放功能的高分子聚合物,其中环境变化主要包括温度、pH值、电刺激等。     

Synthesis of polymer particles with specific lysozyme recognition sites by a molecular imprinting technique

To prepare silica beads covered with a lysozyme‐imprinted polymer layer, we polymerized acrylamide and acrylic acid or acrylamide and N,N‐dimethylaminopropylacrylamide with (NH₄)₂S₂O₈ in a phosphate buffer containing the lysozyme, surface‐modified silica beads, and crosslinkers; the result was the formation of a polymer layer with a lysozyme recognition site on the silica‐bead surface. By quantitative analysis of the supernatant of the solution containing the silica beads, we confirmed that modified silica beads, in contrast to unmodified silica beads, can selectively adsorb lysozymes. The process of binding and releasing the lysozyme to and from the modified silica beads can be repeated several times without degradation of the rebinding ability. A quartz‐crystal microbalance sensor fabricated with a molecularly imprinted polymer layer with a lysozyme recognition site was prepared. When a lysozyme aqueous solution was added to the solution in which the sensor was immersed, a high level of sensitivity and response was observed. High selectivity was also demonstrated by tests with other protein solutions. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 81: 3378–3387, 2001     

Molecularly imprinted polymers: present and future prospective

Molecular Imprinting Technology (MIT) is a technique to design artificial receptors with a predetermined selectivity and specificity for a given analyte, which can be used as ideal materials in various application fields. Molecularly Imprinted Polymers (MIPs), the polymeric matrices obtained using the imprinting technology, are robust molecular recognition elements able to mimic natural recognition entities, such as antibodies and biological receptors, useful to separate and analyze complicated samples such as biological fluids and environmental samples. The scope of this review is to provide a general overview on MIPs field discussing first general aspects in MIP preparation and then dealing with various application aspects. This review aims to outline the molecularly imprinted process and present a summary of principal application fields of molecularly imprinted polymers, focusing on chemical sensing, separation science, drug delivery and catalysis. Some significant aspects about preparation and application of the molecular imprinting polymers with examples taken from the recent literature will be discussed. Theoretical and experimental parameters for MIPs design in terms of the interaction between template and polymer functionalities will be considered and synthesis methods for the improvement of MIP recognition properties will also be presented.     

智能印迹聚合物及其外场强化的识别机制研究进展

智能印迹聚合物在外部环境刺激下对模板分子具有响应性分子识别吸附能力,在吸附分离、药物传递、检测、固相萃取、催化等应用领域有着广阔的前景。本文首先对分子印迹聚合物进行了综述并指出在外场强化过程中常规分子印迹聚合物很难通过改变结合位点来控制结合特性的问题,针对这个问题进而提出具有柔性位点的智能响应型印迹聚合物。随后以具有不同响应功能的智能印迹聚合物为出发点,对磁、热、光、pH、生物大分子等单因子及双重因子响应功能印迹聚合物在外场强化过程中的响应与识别机制分别进行分析与总结,并综述了近些年来这些智能印迹聚合物在不同应用领域中相关研究工作进展。最后,基于不同响应功能的智能印迹聚合物的现状问题,对智能印迹聚合物在材料制备与理论两方面分别进行了展望。     

Metal ion imprinted microsphere prepared by surface molecular imprinting technique using water-in-oil-in-water emulsions

A novel metal ion imprinted polymer was prepared by the surface molecular imprinting technique. Trimethylolpropane trimethacrylate (TRIM), zinc ions, and 1,12-dodecanediol-O,O′-diphenyl phosphonic acid (DDDPA) were used as the matrix-forming monomer, imprint molecule, and functional monomer, respectively. We have prepared Zn(II)-imprinted microspheres utilizing water-in-oil-in-water emulsions, which are spherically well defined and uniform. TRIM comprises of three polymerizable groups that serve to align the recognition sites produced on the polymer surface with better template effect. We conducted diagnostic zinc adsorption tests by using the Zn(II)-imprinted and unimprinted polymers in order to make an assessment on the effectiveness of TRIM-based and divinylbenzene-based imprinted polymers. The template effect of the TRIM-based polymer was enhanced by the high rigidity of the polymer matrix compared to that of the imprinted polymer whose matrix is divinylbenzene. In addition, the adsorption equilibrium constant was evaluated on the basis of the Langmuir analysis for the adsorption data. The method to prepare metal-imprinted microspheres on the surface of polymers crosslinked by TRIM is easy, and the adsorbent produced can be readily used without subsequent grinding or sieving. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 73: 1223–1230, 1999     

Separation of hydroxybenzoic acid isomers using the molecular imprinting technique

Molecular imprinting polymers (MIPs) for salicylic acid (SA), 3‐hydroxybenzoic acid (3HBA), and 4‐hydroxybenzoic acid (4HBA) were synthesized using styrene and 4‐vinylpyridine (4‐VPy) as functional monomer and divinylbenzene (DVB) as crosslinker. The adsorption characteristics of hydroxybenzoic acid (HBA) isomers on each MIP were investigated. The materials used for the polymerization of each 3HBA and 4HBA‐MIPs were adsorbed relatively well. This verifies that the MIPs that can adsorb template selectively were synthesized. However, SA‐MIP had no molecular imprinting effect. SA has intramolecular hydrogen bond and it is difficult to adsorb on recognition site of SA‐MIP, because its structure differs from that of recognition site of SA‐MIP. It indicates that SA‐MIP had no recognition effect. 1,2,3,4‐Tetrahydro‐1‐naphthol (THN)‐MIP was synthesized, which is similar to SA with intramolecular hydrogen bond. SA was separated selectively using THN‐MIP. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007