一种新型医用高氮无镍不锈钢的生物相容性研究

研究了成骨细胞及血小板在不同氮含量的新型高氮无镍奥氏体不锈钢材料表面的粘附及细胞毒性表现。通过血小板粘附实验考察不同氮含量的高氮无镍奥氏体不锈钢材料的血液相容性;通过MTT实验和细胞粘附实验考察不同氮含量的高氮无镍奥氏体不锈钢材料的细胞相容性。结果表明,不同氮含量的高氮无镍奥氏体不锈钢材料对血小板粘附的影响不显著;不同氮含量的无镍不锈钢材料对成骨细胞的生长、形态和增殖不构成伤害,且氮含量对细胞的粘附影响并不显著;细胞毒性实验表明,高氮无镍奥氏体不锈钢和对照组钛合金材料性能稳定,对成骨细胞的生长没有产生明显的毒副作用。     

生物可降解Mg-2Zn-0.2Mn合金的体外生物相容性

采用真空铸造技术制备了Mg-2Zn-0.2Mn三元镁合金,并对该合金体外生物生物相容性能进行了研究。采用血小板粘附、溶血率评价镁合金的血液相容性,采用细胞增殖实验(CCK-8)、细胞凋亡实验(AO/PI)评价镁合金的细胞相容性。结果表明:血小板粘附实验表明,与常用的AZ31B镁合金和316L不锈钢样品相比较,Mg-2Zn0.2Mn镁合金表面粘附的血小板数量少,且激活和团聚较轻微;溶血实验的结果表明Mg-2Zn0.2Mn的溶血率为4.7%,远小于AZ31B的80.9%溶血率。血小板黏附和溶血实验说明Mg-2Zn0.2Mn镁合金具有优异的血液相容性。细胞增殖和凋亡实验结果也表明,较AZ31B样品,Mg-2Zn0.2Mn样品表面显示了更好的内皮细胞粘附和增殖能力,说明Mg-2Zn0.2Mn镁合金具有很好的血液相容性和内皮细胞相容性,有望作为今后可降解血管支架的候选材料。     

超声雾化喷涂法制备抗血小板膜糖蛋白单克隆抗体洗脱支架及其生物相容性研究

采用超声雾化喷涂法制备抗血小板膜糖蛋白单克隆抗体洗脱支架。以涂层均匀度和接触角大小来优化壳聚糖单抗涂层参数,在外层喷涂一层左旋聚乳酸作为屏蔽层控制单抗SZ-21的释放速度。通过溶血率检测涂层支架的血液相容性;MTT实验和细胞粘附实验考察涂层支架的细胞相容性。对支架表面涂层进行SEM和EDS分析,结果表明,聚合物和单抗成功涂层到支架表面,且涂层均匀。涂层支架溶血率低于国家标准的5%,涂层支架对血管内皮细胞没有产生明显的毒副作用;涂层支架有良好的细胞相容性,利于细胞在支架上生长。具有屏蔽层的单抗SZ-21涂层支架较没有涂层屏蔽层的单抗SZ-21涂层支架的SZ-21释放明显成缓慢释放,无暴释现象。     

基材与液晶种类对液晶复合膜血液相容性的影响

从仿生学的角度设计和制备结构及组成与生物膜及血管内壁相似的聚合物/液晶复合膜,作为新一类抗凝血生物材料,并通过血液相容性试验考察复合材料的抗凝血性能.结果显示:不同基材及液晶种类的聚合物/液晶复合膜,其溶血率值均小于5%,符合生物材料溶血试验的要求;复合膜的动态凝血性能取决于基材与液晶两者之间的兼容性及配比,动态凝血性能较佳的复合膜表面粘附的血小板大多呈单个粘附状态,数量较少,且基本无变形,呈现出良好的血液相容性.     

成纤维细胞和血小板在多壁碳纳米管上粘附性

使用化学气相沉积(CVD)的方法在碳纸基底上制备了多壁碳纳米管(MWCNTs),并研究了L929小鼠成纤维细胞在多壁碳纳米管和碳纸对照组上的粘附及增殖等生长行为,以及各种血液蛋白吸附于这两种材料表面后对人皮肤成纤维细胞粘附产生的影响,同时对比了这两种材料的血小板粘附情况.结果表明:种植在多壁碳纳米管上的成纤维细胞生长明显比碳纸上的旺盛,细胞浓度从第1天的12.5×10~5/mL明显增加到第7天的4.1×10~5/mL,多壁碳纳米管对细胞无毒性反应.预吸附白蛋白、纤维蛋白原、免疫球蛋白对细胞粘附量有促进作用,并且多壁碳纳米管的血小板粘附率低于碳纸.这些结果证明多壁碳纳米管具有良好的组织相容性和一定的血液相容性.     

水热反应时间对三维石墨烯血液相容性的影响

以石墨烯为原料,采用乙二胺水热还原的方法制备三维石墨烯,在实验中通过控制水热反应时间来调控所得到的三维石墨烯的孔径大小,并通过扫描电子显微镜(SEM)、X射线光电子能谱(XPS)对三维石墨烯的表面形貌、结构和成分进行了表征,利用蛋白电泳、血小板粘附实验、溶血实验对制备样品表面的蛋白吸附率、血小板粘附等情况进行了对比。随着水热反应时间的延长,三维石墨烯的孔径由340μm逐渐减小为230μm,且N元素被引入到材料中,形成了新的化学键。将水热反应时间由6h延长至24h,使得制备出的三维石墨烯样品表面对牛血清白蛋白(BSA)的吸附量由2.6%上升至16.8%,溶血率由2.2%降至0.3%;同时,抗血小板粘附的能力也在不断增强。水热反应时间的延长可以减小三维石墨烯材料的表面孔径、增加其比表面积,提高材料表面对BSA的吸附能力和抗血小板粘附的能力,同时不断降低材料溶血率。水热反应时间的延长有利于提高石墨烯材料的血液相容性。     

胶原模拟多肽对L929细胞粘附的影响

设计合成了3种模拟胶原三螺旋结构或/和整合素识别位点的胶原模拟多肽(CMP),对其进行体外细胞相容性评价,研究其对小鼠成纤维细胞(L929)生长、粘附的影响。实验证实,3种CMP对成纤维细胞生长无明显的细胞毒性反应;3种包被胶原模拟多肽的基底均能在一定程度上促进细胞粘附、生长,具有良好的细胞粘附率和细胞附着形态,其中包含三螺旋结构和整合素识别位点的CMP27具有更好的促粘附效果,细胞粘附数量和形态与胶原接近。初步研究结果证实,胶原三螺旋结构与整合素识别位点共同作用促进L929细胞粘附。因此,CMP可以有效促进细胞粘附,有望作为粘附剂应用于生物医学领域,可为设计以多肽为基础的生物活性材料提供新的研究思路。     

冷变形对00Cr18Mn15Mo2N0.9高氮无镍不锈钢摩擦磨损性能的影响EI北大核心CSCD

对新型00Cr18Mn15Mo2N0.9高氮无镍不锈钢进行不同变形量的冷轧处理,研究了高氮无镍不锈钢的冷变形性能以及冷变形对其摩擦磨损性能的影响。结果表明,高氮无镍不锈钢的奥氏体组织稳定,即使发生60%的冷变形也不产生形变马氏体;随着冷变形量的增加,高氮无镍不锈钢的强度、硬度提高,断后延伸率、加工硬化指数逐渐减小。在2、5和10 N载荷作用下,00Cr18Mn15Mo2N0.9高氮无镍不锈钢的磨损速率随着冷变形量的增加呈现先减小后增加的趋势,且载荷为2 N和5 N时在20%变形量处高氮无镍不锈钢具有最佳耐磨性,载荷为10 N时40%变形态高氮无镍不锈钢的耐磨性最佳。同时,随着冷变形程度和载荷的增加,00Cr18Mn15Mo2N0.9高氮无镍不锈钢的磨损机制逐渐由磨粒磨损、氧化磨损和脆性剥落转变为磨粒磨损和脆性剥落。     

生物材料对细胞生物学行为的影响

随着细胞生物学、基因工程等研究领域的发展,临床使用的生物医用材料已经由单纯的生物材料制成的植人物,发展成细胞与生物材料的复合物.生物医用材料不仅应具备生物安全性、生物相容性、血液相容性,还应该具有细胞外基质的作用.本文综述了生物材料作为细胞外基质,对细胞粘附、细胞生长及细胞凋亡的影响.     

冷变形对00Cr18Mn15Mo2N0.9高氮无镍不锈钢摩擦磨损性能的影响

对新型00Cr18Mn15Mo2N0.9高氮无镍不锈钢进行不同变形量的冷轧处理,研究了高氮无镍不锈钢的冷变形性能以及冷变形对其摩擦磨损性能的影响。结果表明,高氮无镍不锈钢的奥氏体组织稳定,即使发生60%的冷变形也不产生形变马氏体;随着冷变形量的增加,高氮无镍不锈钢的强度、硬度提高,断后延伸率、加工硬化指数逐渐减小。在2、5和10 N载荷作用下,00Cr18Mn15Mo2N0.9高氮无镍不锈钢的磨损速率随着冷变形量的增加呈现先减小后增加的趋势,且载荷为2 N和5 N时在20%变形量处高氮无镍不锈钢具有最佳耐磨性,载荷为10 N时40%变形态高氮无镍不锈钢的耐磨性最佳。同时,随着冷变形程度和载荷的增加,00Cr18Mn15Mo2N0.9高氮无镍不锈钢的磨损机制逐渐由磨粒磨损、氧化磨损和脆性剥落转变为磨粒磨损和脆性剥落。     

Effect of nitrogen on blood compatibility of nickel-free high nitrogen stainless steel for biomaterial

The aim of this paper was to study the effect of nitrogen content on blood compatibility including platelet adhesion and kinetic clotting time of nickel-free high nitrogen stainless steel (HNS), also in comparison with a conventional austenitic stainless steel AISI 317L. X-ray photoelectron spectroscopy (XPS) was used to analyze the surface chemical composition. The surface wettability and surface free energy (SFE) of these materials were characterized by water contact angle (WCA) measurement to analysis the relationship between surface properties and blood compatibility. Kinetic clotting time was used to evaluate the blood coagulation for these materials and platelet adhesion was assessed by scanning electron microscopy (SEM). The results showed that more platelets adhered on the surface of 317L stainless steel than that on HNS, and with the increase of nitrogen content, the amount of adherent platelets was further decreased on the surface of HNS. Kinetic clotting time results also showed the increased nitrogen content extended the initial clotting time of HNS. The results of surface properties also explained the effect of nitrogen on blood compatibility by traditional theory of SFE and interfacial energy.     

In vitro study of platelet adhesion on medical nickel-free stainless steel surface

A new nickel-free austenitic stainless steel, named BIOSSN4, has been recently developed to avoid the sensitivity to Ni ions. In the present study, the blood compatibility of the nickel-free stainless steel was evaluated in vitro by the platelet-rich plasma adhesion test with comparison to 316L stainless steel. The result shows that the nickel-free stainless steel only causes less activation of platelets, which was indicated by their morphology and low spreading. The result suggests that the nickel-free stainless steel should have better blood compatibility compared with 316L stainless steel and, as a bio-material, it should have obvious advantage and potential applications.     

磁控溅射制备TiO2薄膜对医用NiTi合金弹簧圈的表面改性

采用非平衡磁控溅射法制备了3种TiO2薄膜,对医用NiTi合金弹簧圈进行了表面改性处理.运用X射线衍射(XRD)、X射线光电子能谱仪(XPS)、原子力显微镜(AFM)﹑扫描电子显微镜(SEM)等手段系统研究了薄膜的表面结构、成分、微观形貌等,同时对薄膜的接触角进行了测试.通过血小板粘附和人脐静脉内皮细胞种植试验研究和评价了薄膜的血栓形成能力和内皮化性能.结果表明,医用NiTi合金弹簧圈表面镀一定结构和性质的TiO2薄膜后,其血栓形成能力和内皮化性能得到明显提高.     

医用金属材料表面含磁粉的TiO2薄膜的制备及其血液相容性

用溶胶-凝胶法在316L不锈钢及镍钛合金基片上制备含SrFe12O19磁性粉末的TiO2薄膜,分析了膜基的结合强度,并用X射线衍射仪(XRD)、扫描电镜(SEM)等对磁性粉末的表S面结构及形貌进行了分析.动态凝血时间和溶血率的测定结果表明用含磁粉的TiO2薄膜进行表面改性的316L不锈钢和NiTi合金动态凝血时间延长,溶血率下降,证明含磁粉的TiO2薄膜有很好的血液相溶性.     

Biocorrosion properties and blood and cell compatibility of pure iron as a biodegradable biomaterial

Biocorrosion properties and blood- and cell compatibility of pure iron were studied in comparison with 316L stainless steel and Mg–Mn–Zn magnesium alloy to reveal the possibility of pure iron as a biodegradable biomaterial. Both electrochemical and weight loss tests showed that pure iron showed a relatively high corrosion rate at the first several days and then decreased to a low level during the following immersion due to the formation of phosphates on the surface. However, the corrosion of pure iron did not cause significant increase in pH value to the solution. In comparison with 316L and Mg–Mn–Zn alloy, the pure iron exhibited biodegradable property in a moderate corrosion rate. Pure iron possessed similar dynamic blood clotting time, prothrombin time and plasma recalcification time to 316L and Mg–Mn–Zn alloy, but a lower hemolysis ratio and a significant lower number density of adhered platelets. MTT results revealed that the extract except the one with 25% 24 h extract actually displayed toxicity to cells and the toxicity increased with the increasing of the iron ion concentration and the incubation time. It was thought there should be an iron ion concentration threshold in the effect of iron ion on the cell toxicity.     

医用不锈钢的细胞膜仿生表面修饰

利用2-（甲基丙烯酰氧基）乙基-2-（三甲基氨基）乙基磷酸酯（MPC）与316L不锈钢表面上γ-氨基丙基三乙氧基硅烷（KH550）的Michael加成反应,将MPC化学接枝到不锈钢的表面．修饰表面的XPS结果证实了MPC的成功接枝,表明在不锈钢表面构建了仿细胞膜表面．体外血小板粘附实验显示,修饰表面具有明显阻抗血小板的粘附、聚集和激活性能,并具有良好的血液相容性．     

316L不锈钢和NiTi合金微磁场表面粗糙度对血液相容性的影响

在316L不锈钢、NiTi合金的含SrFe12O19磁性粉末的TiO2薄膜表面用溶胶-凝胶法再涂覆不同层数的TiO2薄膜,以降低材料微磁场表面的微粗糙度,并用扫描电镜、粗糙度仪分析薄膜的表面粗糙度.测试了不同粗糙度的微磁场表面的动态凝血时间和溶血率,研究了微磁场表面的粗糙度对材料血液相容性的影响.结果表明,粗糙度小的微磁场表面的血液相容性比粗糙度大的微磁场表面的血液相容性好.即对于平整光滑的微磁场表面,可以利用微磁场提高材料血液相容性的同时,进一步改善材料的血液相容性.     

Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behaviour

In this communication, we report a facile nanotopographical control over a stainless steel surface via an electrophoretic deposition of colloidal amphiphilic chitosan for preferential growth, proliferation or migration of vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs). Atomic force microscopy revealed that the colloidal surface exhibited a deposition time-dependent nanotopographical evolution, wherein two different nanotopographic textures indexed by ‘kurtosis’ (R_kur) value were easily designed, which were termed as ‘sharp’ (i.e. high peak-to-valley texture) surface and ‘flat’ (i.e. low peak-to-valley texture) surface. Cellular behaviour of VSMCs and HUVECs on both surfaces demonstrated topographically dependent morphogenesis, adherent responses and biochemical properties in comparison with bare stainless steel. The formation of a biofunctionalized surface upon a facile colloidal chitosan deposition envisions the potential application towards numerous biomedical devices, and this is especially promising for cardiovascular stents wherein a new surface with optimized texture can be designed and is expected to create an advantageous environment to stimulate HUVEC growth for improved healing performance.     

Hemocompatibility of lanthanum oxide films fabricated by dual plasma deposition

Lanthanum oxide films were fabricated using dual plasma deposition. X-ray photoelectron spectroscopy (XPS) showed that La existed in the + 3 oxidation state. X-ray diffraction (XRD) revealed a (101) oriented hexagonal structure. Blood platelet adhesion tests and endothelial cell cultures were used to evaluate the hemocompatibility of the as-deposited films. Scanning electron microscopy (SEM) and optical microscopy were employed to evaluate the surface morphology of the blood platelets and endothelial cells on the films. The results showed that the number of adhered, aggregated and morphologically changed platelets was reduced compared to that observed on low-temperature isotropic carbon (LTIC). Endothelial cells culture tests indicated good adhesion and proliferation of human umbilical vein endothelial (HUVE) cells in vitro. Our study suggests that lanthanum oxide films are potential blood-contacting biomedical materials.     

Nickel-free Stainless Steel for Medical Applications

BIOSS4 steel is essentially a nickel-free austenitic stainless steel developed by the Institute of Metal Research, Chinese Academy of Sciences, in response to nickel allergy problems associated with nickel-containing stainless steels that are widely used in medical applications. The high nitrogen content of this steel effectively maintains the austenitic stability and also contributes to the high levels of corrosion resistance and strength. BIOSS4 steel possesses a good combination of high strength and toughness, better corrosion resistance, and better blood compatibility, in comparison with the medical 316L stainless steel. Potential applications of BIOSS4 steel can include medical implantation material and orthodontic or orthopedic devices, as well as jewelries and other decorations.