Three-dimensional shape measurement of a diffusing surface by use of a femtosecond amplifying optical Kerr gate

We propose a system for three-dimensional (3D) shape measurement of a diffusing surface by use of a previously developed femtosecond amplifying optical Kerr gate (fs-amp OKG). The system has an opening time of 459 fs and a maximum transmittance of 185%. It also provides good 3D imaging performance: a transverse imaging resolution of 70 mum, a depth resolution of 100 mum, and a positioning accuracy of 5.9 mum in depth. It is found that the optical Kerr effect and the amplification process in the fs-amp OKG do not cause the quality of a time-resolved image to deteriorate. We prove the effectiveness of the proposed system by measuring the shapes of completely diffusing objects with stepped and spherical surfaces.     

Ultrafast optical Kerr gate of bismuth–plumbum oxide glass for time-gated ballistic imaging

We demonstrated the time-gated ballistic imaging technique using a femtosecond optical Kerr gate (OKG) of bismuth–plumbum oxide glass, the nonlinear optical properties of which were also investigated. The third-order nonlinear refractive-index n₂ of the bismuth–plumbum oxide glass was measured to be 2.19?×?10^?15?cm²/W, and the nonlinear response time was estimated to be shorter than 180?fs. For the time-gated ballistic imaging, the maximum measurable optical density of turbid media using the OKG of bismuth–plumbum oxide glass was 9.3, while only 7.0 for the OKG of quartz glass. And the intensities of the images for the bismuth–plumbum oxide glass were approximately two orders of magnitude higher than that for the quartz glass. The experimental results indicated that the bismuth–plumbum oxide glass was an excellent optical material for nonlinear optical applications.     

Ultrafast optical Kerr gate imaging in a poly-disperse turbid medium

The influence of the size parameter of the scatterers on ultrafast optical Kerr gate (OKG) imaging is investigated in highly scattering poly-disperse turbid media. The results show that in a poly-disperse turbid medium, which in our case, is a suspension of two different sized mono-disperse microspheres, the temporal and spatial behaviors of the light pulses transmitted through it are dominated by the smaller microspheres. The contrasts of the OKG images for the poly-disperse microsphere sample are closer to the contrasts of the OKG images for the smaller sized mono-disperse microsphere sample.     

Parallel optical coherence tomography system

We present the design and procedures for implementing a parallel optical coherence tomography (POCT) imaging system that can be adapted to an endoscopic format. The POCT system consists of a single mode fiber (SMF) array with multiple reduced diameter (15 microm) SMFs in the sample arm with 15 microm center spacing between fibers. The size of the array determines the size of the transverse imaging field. Electronic scanning eliminates the need for mechanically scanning in the lateral direction. Experimental image data obtained with this system show the capability for parallel axial scan acquisition with lateral resolution comparable to mechanically scanned optical coherence tomography systems.     

Kerr effect imaging of dynamic processes in magnetic recordingheads

Techniques for imaging microscopic dynamic magnetic phenomena in magnetic recording heads are reviewed. Two experimental apparatus which utilize the Kerr magnetooptic effect are described. A scanning magnetooptic photometer uses the principles of confocal optical microscopy in which a focused laser spot serves as a high-resolution (~0.3 μm) probe of magnetic activity to very high frequencies (250 MHz). Magnetooptic flash photography uses the technique of stroboscopic imaging with digital image processing to provide instantaneous (10 ns exposure time) images of magnetic phenomena on a microscopic scale by utilizing a pulse laser for illumination. Results from various studies of ferrite, metal-in-gap, and thin-film magnetic recording heads using these apparatus are reviewed along with their methods     

Three-dimensional time-resolved optical tomography of a conical breast phantom

A 32-channel time-resolved imaging device for medical optical tomography has been employed to evaluate a scheme for imaging the human female breast. The fully automated instrument and the reconstruction procedure have been tested on a conical phantom with tissue-equivalent optical properties. The imaging protocol has been designed to obviate compression of the breast and the need for coupling fluids. Images are generated from experimental data with an iterative reconstruction algorithm that employs a three-dimensional (3D) finite-element diffusion-based forward model. Embedded regions with twice the background optical properties are revealed in separate 3D absorption and scattering images of the phantom. The implications for 3D time-resolved optical tomography of the breast are discussed.     

Two-dimensional photoacoustic imaging by use of Fourier-transform image reconstruction and a detector with an anisotropic response

Theoretical and experimental aspects of two-dimensional (2D) biomedical photoacoustic imaging have been investigated. A 2D Fourier-transform-based reconstruction algorithm that is significantly faster and produces fewer artifacts than simple radial backprojection methods is described. The image-reconstruction time for a 208 x 482 pixel image is approximately 1 s. For the practical implementation of 2D photoacoustic imaging, a rectangular detector geometry was used to obtain an anisotropic detection sensitivity in order to reject out-of-plane signals, thereby permitting a tomographic image slice to be reconstructed. This approach was investigated by the numerical modeling of the broadband directional response of a rectangular detector and imaging of various spatially calibrated absorbing targets immersed in a turbid phantom. The experimental setup was based on a Q-switched Nd:YAG excitation laser source and a mechanically line-scanned Fabry-Perot polymer-film ultrasound sensor. For a 800 microm x 200 microm rectangular detector, the reconstructed image slice thickness was 0.8 mm up to a vertical distance of z = 3.5 mm from the detector, increasing thereafter to 2 mm at z = 10 mm. Horizontal and vertical spatial resolutions within the reconstructed slice were approximately 200 and 60 microm, respectively.     

Multiple-slice imaging of a tissue-equivalent phantom by use of time-resolved optical tomography

Following several years of development the construction of a multichannel time-resolved imaging device for medical optical tomography has been completed. Images are reconstructed from time-resolved measurements by use of a scheme that employs a finite-element diffusion-based forward model and an iterative reconstruction solver. Prior to testing on clinical subjects the fully automated instrument and the reconstruction software are evaluated with tissue-equivalent phantoms. We describe our first attempt to generate multiple-slice images of a phantom without uniform properties along the axial direction, while still using a computationally fast two-dimensional reconstruction algorithm. The image quality is improved by the employment of an approximate correction method that uses scaling factors derived from the ratios of finite-element forward simulations in two and three spatial dimensions. The 32-channel system was employed to generate maps of the internal scattering and the absorption properties at 14 different transverse planes across the phantom. The images clearly reveal the locations of small inhomogeneous regions embedded within the phantom. These results were obtained by use of purely temporal data and without resource to reference measurements.     

Two-dimensional imaging biosensor for the monitoring of lactate released from brain slices

Real-time monitoring of lactate release from brain slices has been studied with an optical two-dimensional (2D) imaging biosensor. The 2D biosensor is prepared by direct immobilization of lactate dehydrogenase (LDH) molecules onto a flat silica glass surface through a covalent binding mechanism. The biosensor is able to spatially differentiate lactate concentration variations with conventional optical microscopic spatial resolution. This biosensor has the capability to effectively detect lactate down to a concentration of 100 nM. The 2D biosensor responds uniformly with 2.5% RSD from pixel to pixel. With a 100 ms response time, this 2D biosensor has the capability of monitoring simultaneously many cells in one image. We have studied the impact of KCI on lactate release from brain slices. Clear differences have been observed in lactate release for different regions of the tissue. The real-time determination of the newly released lactate from the mouse brain slices clearly demonstrates the feasibility of monitoring lactate release from living specimens. The 2D biosensor will enable us to study cellular communications and possibly other biological processes that require simultaneous temporal and spatial resolution.     

Imaging with diffusing light: an experimental study of the effect of background optical properties

The overall image quality and diagnostic potential of time-resolved transmittance imaging depend on sensitivity to optical contrast, capacity to discriminate scattering from absorption contributions, and spatial resolution. We have investigated experimentally the effects of the optical properties of the background medium on the overall image quality of optical imaging based on fitting the experimental data to the solution of the diffusion equation and on time gating. Images were acquired from phantoms with different background optical properties, while the optical contrast between inhomogeneities and background is kept constant. Data were collected every 0.2 cm over a 6 cm x 6 cm area from realistic tissue phantoms containing cylindrical inhomogeneities (1 cm high and 1 cm in diameter) embedded in a 5-cm-thick turbid slab. The optical coefficients of the background were varied in the ranges of 5-15 cm(-1) for transport scattering and 0.02-0.08 cm(-1) for absorption. The optical contrast for the inclusions was kept at values of -50% and +50% for the scattering and -75% and +300% for the absorption. The results show that both high scattering and high absorption are beneficial.     

Real-time three-dimensional object recognition with multiple perspectives imaging

A novel system for recognizing three-dimensional (3D) objects by use of multiple perspectives imaging is proposed. A 3D object under incoherent illumination is projected into an array of two-dimensional (2D) elemental images by use of a microlens array. Each elemental 2D image corresponds to a different perspective of the 3D object. Multiple perspectives imaging based on integral photography has been used for 3D display. In this way, the whole set of 2D elemental images records 3D information about the input object. After an optical incoherent-to-coherent conversion, an optical processor is employed to perform the correlation between the input and the reference 3D objects. Use of micro-optics allows us to process the 3D information in real time and with a compact optical system. To the best of our knowledge this 3D processor is the first to apply the principle of integral photography to 3D image recognition. We present experimental results obtained with both a digital and an optical implementation of the system. We also show that the system can recognize a slightly out-of-plane rotated 3D object.     

Backward-mode multiwavelength photoacoustic scanner using a planar Fabry-Perot polymer film ultrasound sensor for high-resolution three-dimensional imaging of biological tissues

A multiwavelength backward-mode planar photoacoustic scanner for 3D imaging of soft tissues to depths of several millimeters with a spatial resolution in the tens to hundreds of micrometers range is described. The system comprises a tunable optical parametric oscillator laser system that provides nanosecond laser pulses between 600 and 1200 nm for generating the photoacoustic signals and an optical ultrasound mapping system based upon a Fabry-Perot polymer film sensor for detecting them. The system enables photoacoustic signals to be mapped in 2D over a 50 mm diameter aperture in steps of 10 microm with an optically defined element size of 64 microm. Two sensors were used, one with a 22 microm thick polymer film spacer and the other with a 38 mum thick spacer providing -3 dB acoustic bandwidths of 39 and 22 MHz, respectively. The measured noise equivalent pressure of the 38 microm sensor was 0.21 kPa over a 20 MHz measurement bandwidth. The instrument line-spread function (LSF) was measured as a function of position and the minimum lateral and vertical LSFs found to be 38 and 15 microm, respectively. To demonstrate the ability of the system to provide high-resolution 3D images, a range of absorbing objects were imaged. Among these was a blood vessel phantom that comprised a network of blood filled tubes of diameters ranging from 62 to 300 microm immersed in an optically scattering liquid. In addition, to demonstrate the applicability of the system to spectroscopic imaging, a phantom comprising tubes filled with dyes of different spectral characteristics was imaged at a range of wavelengths. It is considered that this type of instrument may provide a practicable alternative to piezoelectric-based photoacoustic systems for high-resolution structural and functional imaging of the skin microvasculature and other superficial structures.     

Optical Kerr switching technique for the production of a picosecond,multiwavelength CO2 laser pulse

A wavelength-independent method for optical gating, based on the optical Kerr effect, has been demonstrated. Using this method, we produced 100-ps, 10-kW, two-wavelength pulses (10.3 and 10.6 microm) with a signal-to-background ratio contrast of 10(5) by slicing a long CO2 pulse. The capability of gating consecutive pulses separated on a picosecond time scale with this method is also shown.     

Confocal theta line-scanning microscope for imaging human tissues

A confocal reflectance theta line-scanning microscope demonstrates imaging of nuclear and cellular morphology in human skin and oral mucosa in vivo. The illumination and detection are through a divided objective lens pupil, resulting in a theta-microscope configuration. A line is directly scanned in the pupil and descanned onto a linear detector array such that the theta line scanner consists of only seven main optical components. The experimentally measured lateral resolution is 1.0 microm and optical section thickness is 1.7 microm under nominal conditions at 830 nm wavelength. Through full-thickness human epidermis (i.e., in the dermis) the measured lateral resolution is 1.7 microm and the optical section thickness is 9.2 microm. The lateral resolution, sectioning, and image quality in epidermal (epithelial) tissue is comparable to that of point scanning confocal microscopy.     

High-speed path-length scanning with a multiple-pass cavity delay line

Techniques for high-speed delay scanning are important for low-coherence interferometry, optical coherence tomography, pump probe measurements, and other applications. We demonstrate a novel scanning delay line using a multiple-pass cavity. Differential delays are accumulated with each pass so that millimeter delays can be generated with tens of micrometer mirror displacements. With special design criteria, misalignment sensitivity can be dramatically reduced. The system is demonstrated to scan 6 m/s at 2-kHz repetition rates. Real-time optical coherence tomography imaging with 500 pixel images at four frames/s is performed. Using a Cr:forsterite laser source, we obtained axial image resolutions of 6 microm with 92-dB sensitivity.     

Preparation, characterization and femtosecond time-resolved optical Kerr effect of plasma polybenzonitrile derivative films

Plasma-polymerized thin films based on two benzonitrile derivatives, aminobenzonitrile (ABN) and nitrobenzonitrile (NBN), were prepared by r.f. glow discharge techniques. Fourier transform infrared (FTIR) and UV-Visible absorption spectra revealed that extensively conjugated C N double bonds have been formed during plasma polymerization in both derivatives. The films formed are transparent and dark brown in color. Morphological study using a field emission microscope (FEM) indicated that fine and homogeneous films could be obtained at comparatively low discharge powers. The femtosecond time-resolved optical Kerr effect was used to measure the third-order optical nonlinearity. A unique ultrafast response and non-resonant optical Kerr effect of two polybenzonitrile derivative films were observed. Furthermore, the effects of polymer molecular structures on the non-linear optical properties were also tentatively discussed.     

Investigation of nonlinear dynamics in CdS by time-resolved nondegenerate pump–probe system with phase object

The time-resolved nondegenerate pump–probe system with phase object is employed for investigation of nonlinear absorption and refraction dynamics in CdS. The 532?nm laser beam with 21?ps duration is used as the excitation and the laser beams of 600 and 680?nm with 10?ps duration from optical parametric generation are used for probing. The experimental results at both probe wavelengths show free-carrier absorption and large free-carrier refraction along with two-photon absorption and bound electronic optical Kerr effect. By numerically fitting the experimental data based on the nondegenerate pump–probe theory, the nondegenerate two-photon absorption coefficient, the nondegenerate Kerr coefficient, the free-carrier decay time, the free-carrier absorptive cross-section and free-carrier refractive coefficient at different wavelengths are all determined.     

Enhanced three-dimensional deconvolution microscopy using a measured depth-varying point-spread function

We present a technique to systematically measure the change in the blurring function of an optical microscope with distance between the source and the coverglass (the depth) and demonstrate its utility in three-dimensional (3D) deconvolution. By controlling the axial positions of the microscope stage and an optically trapped bead independently, we can record the 3D blurring function at different depths. We find that the peak intensity collected from a single bead decreases with depth and that the width of the axial, but not the lateral, profile increases with depth. We present simple convolution and deconvolution algorithms that use the full depth-varying point-spread functions and use these to demonstrate a reduction of elongation artifacts in a reconstructed image of a 2 microm sphere.     

Two-dimensional synthetic aperture laser optical feedback imaging using galvanometric scanning

We have improved the resolution of our laser optical feedback imaging (LOFI) setup by using a synthetic aperture (SA) process. We report a two-dimensional (2D) SA LOFI experiment where the unprocessed image (i.e., the classical LOFI image) is obtained point by point, line after line using full 2D galvanometric scanning. The 2D superresolved image is then obtained by successively computing two angular SA operations while a one-dimensional angular synthesis is preceded by a frequency synthesis to obtain a 2D superresolved image conventionally in the synthetic aperture radar (SAR) method and their corresponding laser method called synthetic aperture ladar. The numerical and experimental results are compared.     

Femtosecond snapshot imaging of propagating light itself

An ultrafast imaging technique has been developed to visualize directly a light pulse that is propagating in a medium. The method, called femtosecond time-resolved optical polarigraphy (FTOP), senses instantaneous changes in the birefringence within the medium that are induced by the propagation of an intense light. A snapshot sequence composed of each femtosecond probing the pulse delay enables ultrafast propagation dynamics of the intense femtosecond laser pulse in the medium, such as gases and liquids, to be visualized directly. Other examples include the filamentation dynamics in CS2 liquid and the propagation dynamics in air related to the interaction with laser breakdown plasma. FTOP can also be used to extract information on the optical Kerr constant and its decay time in media. This method is useful in the monitoring of the intensity distribution in the nonlinear propagation of intense light pulses, which is a frequently studied subject in the field of physics regarding nonlinear optics and laser processing.