提高酵母精呈味核苷酸（I＋G）含量的研究

本文以新鲜面包酵母为原料，探讨了麦芽根５’—磷酸二酯酶和自溶后ＲＮＡ的提取工艺以及用５’—磷酸二酯酶定向水解ＲＮＡ生成５’—核苷酸的酶解工艺，得出了提高呈味核苷酸含量的最佳工艺条件：麦芽根加７倍水，４℃下提取２０ｈ；自溶后升温到９５℃，调ｐＨ８５，提取ＲＮＡ１００ｍｉｎ；７０℃、ｐＨ５２条件下以１０ｍｌ／１００ｇ自溶液的量加入５’—磷酸二酯酶作用２５ｈ，可使产品酵母精粉中呈味核苷酸（Ｉ＋Ｇ）含量达到１６０９ｍｇ／１００ｇ。     

麦芽根中5′-磷酸二脂酶的提取及制备

要主要研究了麦芽根中5’—磷酸二酯酶的提取方法。采用细度40目的原料，通过正交试验确定最佳提取工艺为：加水6倍，pH5.0，在4℃条件下浸提20h后5’—磷酸二酯酶活力达到160μ/mL。不同浸提液种类、浓度、添加无机盐皆对酶活有影响，加入纤维食酶、果胶酶、viscozyme对麦芽根破壁具有协同作用。酶液经超滤浓缩可制备得率较高的粗酶粉。     

提高酵母抽提物5’-GMP含量的研究

对新鲜麦芽根中5’—磷酸二酯酶的提取及酶解条件进行了研究，并利用所得5‘-磷酸二酯酶应用于酵母抽提物-的生产中以提高5’-GMP的含量。     

麦芽根中5'-磷酸二酯酶的提取及制备

主要研究了麦芽根中 5 ' 磷酸二酯酶的提取方法。采用细度 40目的原料 ,通过正交试验确定最佳提取工艺为 :加水 6倍 ,pH5 .0 ,在 4℃条件下浸提 2 0h后 5′ 磷酸二酯酶活力达到 1 6 0u/mL。不同浸提液种类、浓度、添加无机盐皆对酶活有影响 ,加入纤维素酶、果胶酶、Viscozyme对麦芽根破壁具有协同作用。酶液经超滤浓缩可制备得率较高的粗酶粉     

5''''-磷酸二酯酶的提取

主要研究了桔青霉与麦芽根提取5′-磷酸二酯酶的方法,并对二者进行了比较.用快速登高法确定了麦芽根提取5′-磷酸二酯酶的最优区域.在此区域内浸提的5′-磷酸二酯酶酶活力可达300U/ml.酶液经超滤浓缩、冷冻干燥可制备得率较高的粗酶粉.     

5′-磷酸二酯酶的提取

主要研究了桔青霉与麦芽根提取5′-磷酸二酯酶的方法，并对二者进行了比较。用快速登高法确定了麦芽根提取5′-磷酸二酯酶的最优区域。在此区域内浸提的5′-磷酸二酯酶酶活力可达300U／ml。酶液经超滤浓缩、冷冻干燥可制备得率较高的粗酶粉。     

提高酵母抽提物5''''-GMP含量的研究

对新鲜麦芽根中5-磷酸二酯酶的提取及酶解条件进行了研究,并利用所得5-磷酸二酯酶应用于酵母抽提物的生产中以提高5-GMP的含量。     

麦芽根中5''-磷酸二酯酶的高效提取

研究了从麦芽根中5'-磷酸二酯酶的提取条件,通过正交确定最佳方法:提取pH6,加水比1:8.40℃提取3h后酶活可达537.86U/mL,浸提液中添加缓冲液,无机盐对酶活均有影响;并对酶液性质进行了初步探讨.     

麦芽根中5′-磷酸二酯酶的高效提取

研究了从麦芽根中5＇-磷酸二酯酶的提取条件,通过正交确定最佳方法：提取pH6,加水比1∶8,40℃提取3h后酶活可达537.86U/mL,浸提液中添加缓冲液,无机盐对酶活均有影响;并对酶液性质进行了初步探讨。     

利用发芽大麦提取5′-磷酸二酯酶的初步研究

文章研究了利用发芽的饲料级大麦来提取5′-磷酸二酯酶的工艺条件。试验结果显示,在25℃温度下,发芽64 h,麦芽中5′-磷酸二酯酶活性最高。利用此麦芽提取5′-磷酸二酯酶时,粉碎度为60目,在温度为50℃,提取液pH值为5.0,并且加入15%的NaCl,料液比为1∶10的条件下提取120 min,可以得到酶活力最高的提取酶液。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press