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Xia EQ Wang BW Xu XR Zhu L Song Y Li HB 《International journal of molecular sciences》2011,12(8):5319-5329
Oleanolic acid and ursolic acid are the main active components in fruit of Ligustrum lucidum Ait, and possess anticancer, antimutagenic, anti-inflammatory, antioxidative and antiprotozoal activities. In this study, microwave-assisted extraction of oleanolic acid and ursolic acid from Ligustrum lucidum was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, microwave power, extraction temperature and microwave time, on the extraction efficiencies of oleanolic acid and ursolic acid from Ligustrum lucidum were evaluated. The influence of experimental parameters on the extraction efficiency of ursolic acid was more significant than that of oleanolic acid (p < 0.05). The optimal extraction conditions were 80% ethanol aqueous solution, the ratio of material to liquid was 1:15, and extraction for 30 min at 70 °C under microwave irradiation of 500 W. Under optimal conditions, the yields of oleanolic acid and ursolic acid were 4.4 ± 0.20 mg/g and 5.8 ± 0.15 mg/g, respectively. The results obtained are helpful for the full utilization of Ligustrum lucidum, which also indicated that microwave-assisted extraction is a very useful method for extraction of oleanolic acid and ursolic acid from plant materials. 相似文献
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A quick method for the determination of total cholesterol in blood serum is described. The method uses antimony pentachloride solution as reagent, a chemical not used in quantitative determination of total cholesterol hitherto. A very fast extraction of the cholesterol from the blood serum is assisted by the selective permeability of vyon discs of different solvents. A correlation study between this method and the Kinn-Goldberg method, and an interference study, are included. 相似文献
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建立了同时测定槐花中芦丁、槲皮素和山柰酚含量的高效液相色谱分析方法。采用酸水解提取法和超声提取法提取槐花中芦丁、槲皮素和山柰酚。结果表明,酸水解提取液中检测不到芦丁,槲皮素含量远大于超声提取液中的含量,加标回收率为93.6%~99.2%。 相似文献
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从理论上推导出三元配合物体系用于萃取光度分析时需满足的条件,提出了计算三元配合物萃取光度测定时酸度范围的方法。 相似文献
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依照标准GB/T 3516-2006,分别用直接回流法和索氏抽提法测定天然橡胶丙酮抽出物,结果表明:直接回流法测定天然橡胶丙酮抽出物可以达到索氏抽提法所测定的结果,并且可以显著节约测定时间。 相似文献