超声波辅助酶法提取葡萄皮渣多酚工艺优化

以赤霞珠葡萄皮渣为原料,采用超声波辅助酶法提取多酚化合物。在探讨超声时间、超声功率、酶解pH及酶解温度对葡萄皮渣多酚得率影响的基础上,应用正交试验优化超声波辅助酶法提取葡萄皮渣多酚的工艺条件。结果表明:影响多酚得率的主次因素为超声时间＞酶解温度＞超声功率＞酶解pH,最佳工艺参数为超声时间15 min,超声功率400 W,酶解pH6.0,酶解温度60 ℃,在此工艺条件下多酚得率最高为1.493%±0.0068%。该工艺成本低、简单快速、稳定可行且提取剂环保无公害,因此可以有效替代传统方法提取葡萄皮渣中的多酚。     

双酶法提取赤霞珠酿酒葡萄皮渣白藜芦醇工艺优化及抗氧化性研究

以赤霞珠酿酒葡萄皮渣为原料,研究双酶法（纤维素酶和果胶酶）辅助浸提白藜芦醇工艺的最佳条件及其体外抗氧化活性。通过单因素试验及正交试验考察纤维素酶添加量、果胶酶添加量、酶解温度、酶解时间、液固比对白藜芦醇浸提工艺的影响。结果表明,最佳白藜芦醇浸提工艺为纤维素酶和果胶酶添加量分别为2.5%和1.2%,酶解温度为45 ℃,酶解时间为100 min,液固比为30∶1（mL∶g）。在此优化条件下,白藜芦醇得率为927 μg/g干质量。体外抗氧化试验结果可知,在质量浓度0.1～0.5 mg/mL的范围内,白藜芦醇对DPPH·和·OH的清除作用较好,最大清除率分别达到83.1%和74.0%。     

超声波-微波辅助酶法提取葡萄皮渣水溶性膳食纤维的工艺研究

以贺兰山东麓赤霞珠葡萄皮渣为原料,以可溶性膳食纤维(SDF)得率为研究指标,采用超声波-微波辅助多酶法提取技术,在单因素试验的基础上,采用正交试验对葡萄皮渣水溶性膳食纤维的提取工艺进行优化。单因素试验结果表明,影响SDF得率的主要因素为酶解温度、微波时间、酶解时间、超声时间。正交试验结果表明,最佳工艺组合参数为:超声时间13 min,微波时间为5 min,酶解时间80 min,酶解温度50℃,此工艺条件下葡萄皮渣SDF得率为20.63%。研究结果为葡萄皮渣的废物利用工业化生产提供了理论依据及数据支撑。     

生物酶法提取葡萄皮渣多酚工艺研究

酿酒葡萄皮渣中含有丰富的多酚类物质,具有较高的营养价值和经济价值。本试验以赤霞珠葡萄皮渣为原料,利用纤维素酶提取葡萄皮渣中的多酚物质。通过单因素结合正交实验优选出葡萄皮渣多酚提取的最佳工艺参数为:酶添加量25 mg、酶解pH5、酶解时间2 h、酶解温度50℃。在此工艺条件下提取的葡萄皮渣中多酚得率可达11.238%。与传统溶剂提取法相比,纤维素酶法提取葡萄皮渣中多酚工艺绿色、安全,具有一定的推广应用价值。     

醇溶法提取葡萄皮渣中的多酚物质

用乙醇作浸提剂,对葡萄皮渣中的多酚物质进行浸提,研究了浸提比例、温度、时间、次数和乙醇浓度5个因素对提取效果的影响,得出醇溶法提取葡萄皮渣中多酚物质的最佳工艺参数,即:浸提比例1∶6、温度70℃、时间12h、浸提3次和乙醇含量70%,得率在80%左右,此时葡萄皮渣中多酚物质的总含量为8.41%。同时研究了不同温度对葡萄皮渣中白藜芦醇(Res)提取效果的影响。     

响应面优化酶法辅助提取葡萄叶白藜芦醇工艺

以新疆南部巴州地区赤霞珠葡萄叶为原料,采用酶法辅助提取葡萄叶中的白藜芦醇。通过试验确定了果胶酶-纤维素酶(6:4,m/m)为最佳用酶,在酶添加量、温度、时间、p H单因素试验的基础上,采用响应面分析法(RSM)对酶法辅助提取葡萄叶白藜芦醇工艺进行优化,得到最佳工艺条件为:酶添加量91 mg(酶解5 g葡萄叶),加入p H5的蒸馏水50 m L,在52℃下酶解1.9 h,再加入适量的乙醇溶液[乙醇浓度最终为40%,固液比为1:25(g/m L)],61℃下浸提4 h,在此条件下提取白藜芦醇为16.5233 mg/g。     

纤维素酶法提取花生红衣中的白藜芦醇

目的:研究纤维素酶解法提取花生红衣中白藜芦醇的工艺条件.方法:在单因素试验基础上,采用L9(34)正交试验法对花生红衣白藜芦醇的酶法提取工艺进行优选.以白藜芦醇的得率为参考指标,考察酶用量、酶解温度、酶解时间、酶解pH值和乙醇浓度对花生红表白藜芦醇得率的影响.结果:最佳工艺条件为:纤维素酶与花生红衣粗粉的配比为1:500,酶解温度50℃,酶解pH 5.0,酶解时间90 min,提取溶剂乙醇的体积分数为60%.在此条件下白藜芦醇的得率为1.25%.与传统醇提工艺相比,其提取率提高了4.43倍.结论:该工艺是纤维素酶法提取花生红衣白藜芦醇的最佳工艺.     

纤维素酶-超声波辅助有机溶剂提取山葡萄渣中白藜芦醇的研究

为进一步提高山葡萄渣中白藜芦醇提取效率,研究了纤维素酶-超声波强化处理对山葡萄渣中白藜芦醇提取效果的影响。研究在单因素实验的基础上进行正交实验,分析纤维素酶酶解温度、超声波处理时间、料液比、提取时间对白藜芦醇含量的影响。结果表明:山葡萄渣中白藜芦醇提取的最佳提取工艺条件为:酶解温度55℃,超声处理时间5min,料液比1:30,提取时间4h。在此条件下测得白藜芦醇的含量为1.187mg·g-1。纤维素酶和超声波复合处理提取工艺能更有效地提高山葡萄渣中白藜芦醇含量,此工艺优于纤维素酶、超声波单一因素处理提取工艺。      

纤维素酶-超声波辅助有机溶剂提取山葡萄渣中白藜芦醇的研究

为进一步提高山葡萄渣中白藜芦醇提取效率,研究了纤维素酶-超声波强化处理对山葡萄渣中白藜芦醇提取效果的影响。研究在单因素实验的基础上进行正交实验,分析纤维素酶酶解温度、超声波处理时间、料液比、提取时间对白藜芦醇含量的影响。结果表明:山葡萄渣中白藜芦醇提取的最佳提取工艺条件为:酶解温度55℃,超声处理时间5min,料液比1:30,提取时间4h。在此条件下测得白藜芦醇的含量为1.187mg·g-1。纤维素酶和超声波复合处理提取工艺能更有效地提高山葡萄渣中白藜芦醇含量,此工艺优于纤维素酶、超声波单一因素处理提取工艺。     

酿酒葡萄皮渣中白藜芦醇的提取及抗氧化、抗肿瘤活性研究

为了提高酿酒葡萄皮渣的综合利用价值,开发白藜芦醇新产品,对酿酒葡萄皮渣中白藜芦醇提取工艺进行研究。通过纤维素 酶水解和乙酸乙酯萃取法提取自酿葡萄酒皮渣中的白藜芦醇,利用响应面分析法优化白藜芦醇提取的工艺条件并测定了白藜芦醇的 抗氧化活性及对3种癌细胞（Hela、A549、PC-3）的生长抑制作用。 结果表明,最佳提取工艺条件为酶添加量70 U/μL、体积分数95%乙 醇与葡萄皮渣液固比20∶1（mL∶g）、酶解时间150 min、酶解温度40 ℃,在此优化条件下,提取得到白藜芦醇的提取率为144.13 μg/g; 抗氧化试验结果表明,白藜芦醇具有清除自由基的能力,当样品质量浓度为1.2 mg/mL时,对DPPH和超氧阴离子自由基的清除率分 别为（65.12±3.88）%和（54.13±3.11）%;体外抗肿瘤活性显示,其对Hela、A549和PC-3细胞的生长均具有抑制作用,半抑制浓度（IC50） 值分别为128.29 μmol/L、108.35 μmol/L和25.31 μmol/L。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the     

Chinese CTP Market

According to Printing and Printing Equipment Industries Association of China（PEIAC）＇s statistics to the plate manufucturer in China, in 2013, the actual offset plate production has reached 346 million square meters in China. Among them, the CTP production volume was 245 million square meters, up by 11% than that of last year; the total sales of the CTP plate was 239 million square meters, up by 13%.     

Preparatory Work of Print China 2015 is Going Smoothly

正Held at Guangdong Modern International Exhibition Center,Print China 2015 will cover 7exhibition halls,besides the original Hall No.3,4,5,6,7,the newly built F zone of Hall 3 will be used too.The total area will be140,000 square meters.Hall 3:Offset and large printing equipment,package printing equipment,post press