A Legionnaires' disease outbreak: a water blaster and roof-collected rainwater systems

In February 2006, an outbreak of Legionnaires' disease (LD) was identified in Beachlands, a small, isolated east Auckland suburb. It was investigated through case finding, a case-control study, sampling potential sources of infection and by molecular typing (using sequence-based typing (SBT) of all Legionella pneumophila serogroup 1 (Lp1) isolates). Lp1 was isolated from the respiratory tract of one case, the roof-collected rainwater systems of five households (three associated with cases) and from a water blaster at a nearby marina. All isolates were indistinguishable, exhibiting the same SBT allele pattern. Three LD cases lived within 500m of the water blaster (the fourth case within 1250m) and downwind in prevailing conditions. Another domestic roof-collected rainwater supply contaminated by Lp1 (identical SBT pattern) was incidentally identified in another suburb 4km east of Beachlands. This is the first outbreak of LD linked to roof-collected rainwater supplies and the first isolation of Legionella from these systems in New Zealand. Aerosols containing Legionella discharged to air by the marina water blaster may have infected some cases directly or may have seeded roof-collected rainwater systems. Some cases may have been exposed by contaminated bathroom showers. Roof-collected rainwater systems need appropriate design, careful cleaning and the maintenance of hot water temperatures at a minimum of 60 degrees C to reduce the chances of Legionella multiplying. Further research into the ecology of Legionella in roof-collected rain water systems is indicated.     

Microbial quality of rainwater supplies in developed countries: a review

The current paper examines the reported microbial quality of rainwater supplies. The majority of microbial contamination derives from debris and faecal material deposited on the roof surface, principally from birds. The prevalence and level of contamination varies widely, both in terms of indicator organisms and pathogens. Gastrointestinal pathogens such as Campylobacter spp., Salmonella spp., Cryptosporidium spp. and Giardia spp. have all been isolated from harvested supplies, although these may not always be of types infective to humans. In the UK, there are currently no regulations in respect to the microbial quality of harvested rainwater for non potable use, although there are generally standards relating to the required plumbing to ensure that potable and non potable supplies remain separate.     

The quality of drinking water from private water supplies in Aberdeenshire,UK

The quality of private water supplies within Aberdeenshire sampled between 1992 and 1998 was analysed with respect to the presence of total coliforms (TC), faecal coliforms (FC), and nitrate. Of the approximately 1750 samples analysed, which included multiple samples from larger supply categories, the individual failure rate was 41%, 30% and 15% for TC, FC and nitrate, respectively. A combined failure rate for these samples was 48%. Failure rates on microbiological grounds displayed a seasonal trend being greater during the latter half of the year. Although this observation is likely to be due to a combination of local and regional scale factors, part of the variability in failure rate was explained by a significant positive relationship with rainfall amount. Concentrations of nitrate tended to display an opposite trend with a greater number of failures occurring during the spring period and no relationship with rainfall was immediately apparent. A relatively small number of samples (< 50) failed simultaneously for both coliforms and nitrate suggesting that the mechanism responsible for the contamination differed. A similar failure rate for samples collected directly from the source (i.e. well) compared with those taken from the potable tap (usually kitchen cold water tap) suggests that it is the groundwater source itself that contributes much of the microbiological and nitrate contamination rather than a storage or supply line contamination mechanism. A more frequent and random sampling of category one F supplies suggested a greater overall failure rate, which has its own implications for deciding an appropriate sampling frequency.     

Monitoring of water quality from roof runoff: Interpretation using multivariate analysis

The quality of harvested rainwater used for toilet flushing in a private house in the south-west of France was assessed over a one-year period. Temperature, pH, conductivity, colour, turbidity, anions, cations, alkalinity, total hardness and total organic carbon were screened using standard analytical techniques. Total flora at 22 °C and 36 °C, total coliforms, Escherichia coli and enterococci were analysed. Overall, the collected rainwater had good physicochemical quality but did not meet the requirements for drinking water. The stored rainwater is characterised by low conductivity, hardness and alkalinity compared to mains water. Three widely used bacterial indicators - total coliforms, E. coli and enterococci - were detected in the majority of samples, indicating microbiological contamination of the water. To elucidate factors affecting the rainwater composition, principal component analysis and cluster analysis were applied to the complete data set of 50 observations. Chemical and microbiological parameters fluctuated during the course of the study, with the highest levels of microbiological contamination observed in roof runoffs collected during the summer. E. coli and enterococci occurred simultaneously, and their presence was linked to precipitation. Runoff quality is also unpredictable because it is sensitive to the weather. Cluster analysis differentiated three clusters: ionic composition, parameters linked with the microbiological load and indicators of faecal contamination. In future surveys, parameters from these three groups will be simultaneously monitored to more accurately characterise roof-collected rainwater.     

Potential for potable water savings by combining the use of rainwater and greywater in houses in southern Brazil

Research on rainwater and greywater have been performed all over the world as a way of promoting potable water savings. The main objective of this article is to evaluate the potential for potable water savings by using rainwater and greywater in two houses in southern Brazil. An economic analysis is performed to evaluate the benefits of using rainwater and greywater either separately or together. Results indicate that the potential for potable water savings in both houses range from 33.8% (house B) to 36.6% (house A), considering that water for toilet flushing and washing machine does not need to be potable. By using rainwater, the potable water savings in house A would be 35.5% and in house B, 33.6%. When greywater is considered alone, potable water savings are lower, i.e., 30.4% in house A and 25.6% in house B. As for the use of rainwater and greywater combined, the potable water savings are 36.4% in house A and 33.8% in house B. The three systems that were investigated seem not to be cost effective as the payback periods were very high (above 17 years), but the greywater system was the most attractive one. The main conclusion that can be made from the research is that there needs to be government incentives in order to promote the use of rainwater or greywater in houses in southern Brazil.     

Potential for potable water savings by using rainwater and greywater in a multi-storey residential building in southern Brazil

Studies on the use of rainwater and greywater to promote potable water savings have been performed in different countries. The main objective of this article is to evaluate the potential for potable water savings by using rainwater and greywater in a multi-storey residential building composed of three blocks, located in Florianópolis, southern Brazil. Water end-uses were estimated by applying questionnaires and measuring water flow rates. An economic analysis was performed to evaluate the cost effectiveness of using rainwater and greywater either separately or together. Results show that the average potential for potable water savings range from 39.2% to 42.7% amongst the three blocks, considering that water for toilet flushing, clothes washing and cleaning does not need to be potable. By using rainwater, the potable water savings would actually range from 14.7% to 17.7%. When greywater is considered alone, potable water savings are higher, i.e., ranging from 28.7% to 34.8%. As for the use of rainwater and greywater combined, the potable water savings range from 36.7% to 42.0%. The main conclusion that can be made from the research is that the three systems that were investigated are cost effective as the payback periods were lower than 8 years, but the greywater system was the most cost effective one, followed closely by the rainwater one.     

Rainwater tank capacity and potential for potable water savings by using rainwater in the residential sector of southeastern Brazil

Rainwater has been used in many countries as a way of minimising water availability problems. In Brazil, it has been reported that the potential for potable water savings by using rainwater may range from 48% to 100% depending on the geographic region. In southeastern Brazil, water availability is about 4500 m³ per capita per year, but it is predicted to be lower than 1000 m³ per capita per year from about 2100 onwards. The main objective of this article is to evaluate the potential for potable water savings by using rainwater in 195 cities located in southeastern Brazil. Rainwater tank sizes are also assessed for some cities in order to evaluate the ideal tank capacity as a function of potable water demand and rainwater demand. Results indicate that average potential for potable water savings range from 12% to 79% per year for the cities analysed. Ideal rainwater tank capacities for dwellings with low potable water demand range from about 2000 to 20,000 litres depending on rainwater demand. For dwellings with high potable water demand, ideal rainwater tank capacities range from about 3000 to 7000 litres. The main conclusion drawn from the research is that the average potential for potable water savings in southeastern Brazil is 41%. It was also concluded that rainwater tank capacity has to be determined for each location and dwelling as it depends strongly on potable water demand and rainwater demand.     

Roof-harvested rainwater for potable purposes: Application of solar collector disinfection (SOCO-DIS)

The efficiency of solar disinfection (SODIS), recommended by the World Health Organization, has been determined for rainwater disinfection, and potential benefits and limitations discussed. The limitations of SODIS have now been overcome by the use of solar collector disinfection (SOCO-DIS), for potential use of rainwater as a small-scale potable water supply, especially in developing countries. Rainwater samples collected from the underground storage tanks of a rooftop rainwater harvesting (RWH) system were exposed to different conditions of sunlight radiation in 2-L polyethylene terephthalate bottles in a solar collector with rectangular base and reflective open wings. Total and fecal coliforms were used, together with Escherichia coli and heterotrophic plate counts, as basic microbial and indicator organisms of water quality for disinfection efficiency evaluation. In the SOCO-DIS system, disinfection improved by 20–30% compared with the SODIS system, and rainwater was fully disinfected even under moderate weather conditions, due to the effects of concentrated sunlight radiation and the synergistic effects of thermal and optical inactivation. The SOCO-DIS system was optimized based on the collector configuration and the reflective base: an inclined position led to an increased disinfection efficiency of 10–15%. Microbial inactivation increased by 10–20% simply by reducing the initial pH value of the rainwater to 5. High turbidities also affected the SOCO-DIS system; the disinfection efficiency decreased by 10–15%, which indicated that rainwater needed to be filtered before treatment. The problem of microbial regrowth was significantly reduced in the SOCO-DIS system compared with the SODIS system because of residual sunlight effects. Only total coliform regrowth was detected at higher turbidities. The SOCO-DIS system was ineffective only under poor weather conditions, when longer exposure times or other practical means of reducing the pH were required for the treatment of stored rainwater for potable purposes.     

The effect of roofing material on the quality of harvested rainwater

Due to decreases in the availability and quality of traditional water resources, harvested rainwater is increasingly used for potable and non-potable purposes. In this study, we examined the effect of conventional roofing materials (i.e., asphalt fiberglass shingle, Galvalume^® metal, and concrete tile) and alternative roofing materials (i.e., cool and green) on the quality of harvested rainwater. Results from pilot-scale and full-scale roofs demonstrated that rainwater harvested from any of these roofing materials would require treatment if the consumer wanted to meet United States Environmental Protection Agency primary and secondary drinking water standards or non-potable water reuse guidelines; at a minimum, first-flush diversion, filtration, and disinfection are recommended. Metal roofs are commonly recommended for rainwater harvesting applications, and this study showed that rainwater harvested from metal roofs tends to have lower concentrations of fecal indicator bacteria as compared to other roofing materials. However, concrete tile and cool roofs produced harvested rainwater quality similar to that from the metal roofs, indicating that these roofing materials also are suitable for rainwater harvesting applications. Although the shingle and green roofs produced water quality comparable in many respects to that from the other roofing materials, their dissolved organic carbon concentrations were very high (approximately one order of magnitude higher than what is typical for a finished drinking water in the United States), which might lead to high concentrations of disinfection byproducts after chlorination. Furthermore the concentrations of some metals (e.g., arsenic) in rainwater harvested from the green roof suggest that the quality of commercial growing media should be carefully examined if the harvested rainwater is being considered for domestic use. Hence, roofing material is an important consideration when designing a rainwater catchment.     

Quantitative microbial risk assessment with respect to Campylobacter spp. in toilets flushed with harvested rainwater

In light of increasing pressures on water supplies in some areas, water demand management and water conservation techniques are likely to become increasingly prevalent. In‐house systems using alternatives to mains supplies for nonpotable uses present one such option for reducing potable water demand. This paper, through a formal desk‐based health impact assessment (HIA) and quantitative microbial risk assessment (QMRA), examines one of the possible health implications (Campylobacter infection from toilet flushing) resulting from the use of rainwater harvesting in the home in the United Kingdom. This is investigated using data from the literature and a hypothetical case study population of over 4000 people (based on data for the ‘average’ population in England), with the results being expressed as disability‐adjusted life years (DALYs) (on an annual basis) and placed in context of the ‘tolerable’ risk from drinking‐water supplies.     

Bacteriological, virological and chemical evaluation of a wastewater-aquaculture system

Levels of fecal coliforms (FC), fecal streptococci (FS), Salmonella spp and enteric viruses were monitored in the water, sediment and fish in experimental wastewater-fish ponds near Benton, Arkansas, U.S.A. Concentrations of five heavy metals were also monitored in the fish and wastewater. Concentrations of indicator bacteria were reduced by as much as 99.7% through the series of six ponds which had a calculated total retention time of 72 days. Two filter-feeding species of Chinese carp, Hypophthalmichthys molitrix (silver carp) and Aristichthys nobilis (bighead carp), grown in the last three ponds accumulated FC and FS in their digestive tracts and skin at levels as great or greater than in the surrounding water and sediment. Only low levels of FC and FS were found in the fish muscle tissue (maximum of 25 FS per 100 g) even when concentrations of bacteria in the gut exceeded 10⁵ per 100 g. Concentrations of bacteria in the water and sediment were not good predictors of concentrations in the fish. No Salmonella and no enteric viruses were isolated from the fish, but this lack of isolates was attributed to the extremely low levels which were present in the influent wastewater. Higher levels of copper and mercury were found in the fish flesh than in the surrounding water, with three of eleven fish samples containing higher than acceptable levels of mercury in the edible portion. Based on the efficiency of wastewater treatment, an aquaculture system using silver and bighead carp was judged to be a viable treatment system for domestic sewage resulting in a product suitable for animal or human consumption if proper precautions are taken in harvesting and processing the fish.     

Rainwater harvesting, quality assessment and utilization in Kefalonia Island, Greece

The quality of harvested rainwater which is used for domestic and drinking purposes in the northern area of Kefalonia Island in SW Greece and the factors affecting it were assessed through 3-year surveillance. In 12 seasonal samplings, 156 rainwater and 144 ground- or mixed water samples were collected from ferroconcrete storage tanks (300-1000 m3 capacity), which are adjacent to cement-paved catchment areas (600-3000 m2). Common anions and major cations as well as the metals Fe, Mn, Cd, Pb, Cu, Cr, Ni and Zn were tested. The presence of three major groups of organic compounds, polycyclic aromatic hydrocarbons (PAHs), organochloride pesticides (OCPs) and volatile organic compounds (VOCs), was screened by common analytical techniques. All of the rainwater samples were within the guidelines for chemical parameters established by the 98/93/EU directive. As far as microbiological quality is concerned, total coliforms, Escherichia coli and enterococci were detected in 80.3%, 40.9% and 28.8% of the rainwater samples, respectively, although they were found in low concentrations. Chemical and microbiological parameters showed seasonal fluctuations. Principal component analysis revealed that microbiological parameters were affected mainly by the cleanness level of catchment areas, while chemical parameters were influenced by the sea proximity and human activities. Disinfection should be applied into the tanker trucks which distribute the water to the consumers and not into the big storage tanks in order to avoid by-products formation. Due to the lack of fluoride in rainwater samples, the consumers must become aware of the fact that the supplementation of this element is needed.     

Physicochemical and microbiological quality of harvested rainwater from an agricultural installation in Ireland

Dublin Institute of Technology was commissioned in 2005 by the Department of Environment, Heritage and Local Government in Ireland to assess the feasibility of utilising rainwater to replace treated mains water for nonpotable uses. The project involved the design, installation, commissioning and monitoring of rainwater harvesting on a farm. Two monitoring programmes, Regime 1 and Regime 2, examined the physicochemical and microbiological quality of the harvested rainwater. Samples were taken monthly and tested. Regime 1 analysis showed that the microbiological quality of the rainwater from the site did not comply with the requirements of the European Communities Quality of Bathing Water Regulations, while the physicochemical quality complied with both Bathing and Drinking Water Standards with the exception of ammonia and lead. Regime 2 results showed a significant improvement and were compliant with the European Communities Quality of Bathing Water Regulations and showed near compliance with the European Communities Drinking Water Regulation.     

Rooftop runoff as a source of contamination: A review

Scientific reports concerning chemical and microbiological contaminant levels of rainwater runoff from rooftop collection in both urban and rural areas are reviewed. This alternative source of water has been documented to often contain substantial amounts of contaminants. Studies describing levels of heavy metal contamination specific to runoff from rooftop catchment areas containing exposed metal surfaces are discussed. Depending upon the intended use, scientific evidence is also accumulating that various treatments and disinfections will be required prior to release of roof-runoff water either into surface waters or for more direct consumer usage. For microbial contamination, current proposed standards and guidelines regarding this type of water source are shown to vary widely worldwide. Scientific literature reveals a lack of clarity regarding water quality guidelines and health related standards for certain types of rooftop runoff. Studies suggests that rainwater collection systems which are properly designed, maintained, and treated may provide a valuable supplement to existing water supplies by reducing demand on community water supplies/infrastructure costs, enhancing effective management of storm water runoff, and increasing restoration of underground reservoirs through controlled infiltration.     

Comparison of bacterial indicator analysis methods in stormwater-affected coastal waters

Membrane filtration (MF) and multiple tube fermentation (MTF) have been used for decades to measure indicator bacteria levels in beach water samples, but new methods based on chromogenic substrate (CS) technology are becoming increasingly popular. Only a few studies have compared results among these methods and they have generally been based on samples collected from a limited number of sites during dry weather. In this study, samples were collected from 108 sites the day after a major rainstorm, and three indicator bacteria (total coliforms (TCs), fecal coliforms (FCs) or E. coli, and enterococci (EC)) were each measured using MF, MTF, and CS. Sampling sites were selected using a stratified random design, stratified by open sandy beach, rocky shoreline, and beach areas near urban runoff outlets. The CS results were found to be highly correlated with both MF and MTF for all three indicators regardless of whether the samples were taken along open shoreline or near a runoff outlet. While correlated, TC values were higher using the CS method, consistent with other studies that have demonstrated false positives with this method. FC values were 12% lower with CS, reflecting the specificity of the CS method for E. coli rather than for the entire FC group. No significant differences were observed for EC, although some differences were observed within specific laboratories. Differences for all of these indicators were small enough that, when assessed categorically, there was more than 90% agreement between CS methods and either MF or MTF methods as to whether State of California Beach Water Quality Standards were met or exceeded.     

Microbiological surveillance of private water supplies in England - The impact of environmental and climate factors on water quality

A passive surveillance system captured information on 34,904 microbiological samples from 11,233 private drinking water supplies within England as well as the associated constructional, climatic and environmental variables. Escherichia coli was detected in 6588 (18.87%) of samples and at least one positive sample was detected from 3638 (32.39%) of sites. However, this estimate of supplies failing to meet the European drinking water E. coli standard was probably an underestimate as the more samples taken per supply, the more likely the supply was to fail. A multivariable model of private water supplies data showed a strong seasonal impact, with samples between January and May being significantly less contaminated with E. coli than samples between June and December. Samples from springs (OR 2.5, CI 2.0-3.1) or surface waters (OR 2.4, CI 0.8-7.0) were more likely to fail than groundwater sources, as were supplies with no effective treatment (OR1.8, CI 1.5-2.3). Commercial supplies were less likely to fail than domestic supplies (OR 0.63, CI 0.48-0.83) and the probability of failure was linearly associated with the density of sheep in the area and rainfall on the previous day. A Monte Carlo modelling approach was used to estimate that, had sufficient samples been taken, 54% (95% confidence intervals 49-59%) of all private water supplies in England were likely to be unsatisfactory. These findings will be able to inform risk assessments of private water supplies prior to microbiological results being available.     

西南岩溶山区集雨工程现状与对策

西南岩溶山区普遍开展了雨水集蓄与利用，有效地解决了该地区人畜饮水和灌溉用水，但是也存在很多问题，如选址布局不合理、设计施工不严格、管理养护不当、雨水利用效率低等，造成了水柜不能收集到足够的雨水(或出现裂缝)、雨水对人体健康产生危害、雨水资源浪费严重等问题，对此进行了分析，并提出了相应的对策。     

The perceived barriers to the inclusion of rainwater harvesting systems by UK house building companies

This work investigates the barriers that exist to deter the implementation of rainwater harvesting into new UK housing. A postal questionnaire was sent to a selection of large, medium and small house-builders distributed across the UK. Questions were asked concerning potential barriers to the inclusion of rainwater harvesting in homes separated into five sections; (1) institutional and regulatory gaps, (2) economic and financial constraints, (3) absence of incentives, (4) lack of information and technical knowledge, and (5) house-builder attitudes. The study concludes that although the knowledge of rainwater systems has increased these barriers are deterring house-builders from installing rainwater harvesting systems in new homes. It is further acknowledged that the implementation of rainwater harvesting will continue to be limited whilst these barriers remain and unless resolved, rainwater harvesting's potential to reduce the consumption of potable water in houses will continue to be limited.     

Fate of indicator microorganisms, Giardia and Cryptosporidium in subsurface flow constructed wetlands

Limited information is available on the ability of subsurface flow wetlands to remove enteric pathogens. Two multi-species wetlands, one receiving secondary sewage effluent and the other potable (disinfected) groundwater were studied from February 1995 to August 1996, at the Pima County Constructed Ecosystems Research Facility in Tucson, Arizona. Each wetland had a retention time of approximately 4 days. The objectives of this study were (1) to evaluate the ability of multi-species subsurface wetlands to physically remove Giardia cysts; Cryptosporidium oocysts, total and fecal coliforms, and coliphages; and (2) to determine the likely impact of local wildlife on the occurrence of these indicators and pathogens. In the wetland receiving secondary sewage effluent, total coliforms were reduced by an average of 98.8% and fecal coliforms by 98.2%. Coliphage were reduced by an average of 95.2%. Both Giardia cysts and Cryptosporidium oocysts were reduced by an average of 87.8 and 64.2%, respectively. In the wetland receiving disinfected groundwater, an average of 1.3 x 10(2) total coliforms/100 mL and 22.3 fecal coliforms/100 mL were most likely contributed by both flora and fauna. No parasites or coliphages were detected.