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1.
11-溴代十一酸是尼龙-11单体的中间体,以10-十一烯酸和溴化氢为原料合成11-溴代十一酸,通过实验得出溴代反应的最佳工艺条件为:引发剂用量2%,溴代温度0-15℃,反应时间1h,溶剂为甲苯,10-十一烯酸与甲苯的最佳配比20%,上述条件下,溴代反应的收率达92%以上。 相似文献
2.
通过三步反应合成5-氯-2-甲基-3-异噻只酮。a)丙烯酸甲酯、多硫化钠、亚硫酸钠在0℃~5℃反应5h得二硫代丙烯酸甲酯,收率90%;b)二硫代丙烯酸甲酯、甲苯、甲醇混合液在15℃~20℃下滴加甲胺反应10h得N,N-二甲基0-二硫代丙烯酰胺,收率100%;c)N,N-二甲基-二硫代丙烯酰胺、二氯乙烷混合液在25℃滴加二氯砜升温反应15h,经过后处理得5-氯-2-甲基-3-异噻唑酮。 相似文献
3.
5—甲基—2—氨基—1,3,4—噻二唑的合成 总被引:3,自引:0,他引:3
本文报道了用硫代氨基脲与乙酸在盐酸、磷酸、多聚磷酸、硫酸等催化下,合成5-甲基-2-氨基-1,3,4-噻二唑,并对工艺条件进行了实验。结果表明,最佳工艺条件为硫代氨基脲与乙酸的摩尔比为1:1.4,浓盐酸催化,回流3小时,产率为74.34%。 相似文献
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光敏剂N-丁基-2-乙氧基硫代吖啶酮的合成 总被引:1,自引:0,他引:1
以邻氯苯甲酸和对乙氧基苯胺为原料,经过胺的苯基化、二芳胺的环化、N-烷基化、硫代化反应合成了N-丁基-2-乙氧基硫代吖啶酮。各步中间产物和目标产物的结构均经红外光谱和质谱确认,通过正交设计实验,得出环化反应的最佳反应条件:温度为100℃,时间为30min,浓硫酸为2-羧基-4′-乙氧基二苯胺的配比为5:1(mL/g),在此条件下反应收率达78.6%。N-丁基-2-乙氧基硫代吖啶酮的光吸收可延伸到540 nm,在可见光区的最大吸收峰为503nm. 相似文献
7.
以4-羟基-6-甲基-2-吡喃酮和对氯苯甲酰氯为起始原料,经过缩合、转位、酯化等五步反应,制得氧代烟酸,五步总收率37.6%。 相似文献
8.
以对溴甲苯为原料,N-溴代丁二酰亚胺为溴化剂,利用光引发的自由基反应合成了1-溴-4-溴甲基苯,产率可达80%,并利用它制得了一个新的有机金属前体。 相似文献
9.
以棕榈酸为起始原料,合成了具有强抗炎活性的β-棕榈酰氨基乙醇异烟酸酯,合成路线简捷,操作简便,反应条件温和,原料易得,总收率(以棕榈酸计)可达77%。 相似文献
10.
报道了4,4′-硫代双[2-(1,1-二甲基乙基)-5一甲基苯酚]的合成方法及结构鉴定。 相似文献
11.
Dr. Alexander J. M. Disney Prof. Barrie Kellam Dr. Lodewijk V. Dekker 《ChemMedChem》2016,11(9):972-979
The natural product staurosporine is a high‐affinity inhibitor of nearly all mammalian protein kinases. The labelling of staurosporine has proven effective as a means of generating protein kinase research tools. Most tools have been generated by acylation of the 4′‐methylamine of the sugar moiety of staurosporine. Herein we describe the alkylation of this group as a first step to generate a fluorescently labelled staurosporine. Following alkylation, a polyethylene glycol linker was installed, allowing subsequent attachment of fluorescein. We report that this fluorescein–staurosporine conjugate binds to cAMP‐dependent protein kinase in the nanomolar range. Furthermore, its binding can be antagonised with unmodified staurosporine as well as ATP, indicating it targets the ATP binding site in a similar fashion to native staurosporine. This reagent has potential application as a screening tool for protein kinases of interest. 相似文献
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Herwig Prasch Dr. Andreas Wolfsgruber Dr. Martin Thonhofer André Culum Christoph Mandl Dr. Patrick Weber Melanie Zündel Dr. Seyed A. Nasseri Dr. Andres Gonzalez Santana Dr. Gregor Tegl Prof. Dr. Bernd Nidetzky Prof. Dr. Karl Gruber Prof. Dr. Arnold E. Stütz Prof. Dr. Stephen G. Withers Prof. Dr. Tanja M. Wrodnigg 《Chembiochem : a European journal of chemical biology》2023,24(23):e202300480
Selective covalent labelling of enzymes using small molecule probes has advanced the scopes of protein profiling. The covalent bond formation to a specific target is the key step of activity-based protein profiling (ABPP), a method which has become an indispensable tool for measuring enzyme activity in complex matrices. With respect to carbohydrate processing enzymes, strategies for ABPP so far involve labelling the active site of the enzyme, which results in permanent loss of activity. Here, we report in a proof of concept study the use of ligand-directed chemistry (LDC) for labelling glycoside hydrolases near – but not in – the active site. During the labelling process, the competitive inhibitor is cleaved from the probe, departs the active site and the enzyme maintains its catalytic activity. To this end, we designed a building block synthetic concept for small molecule probes containing iminosugar-based reversible inhibitors for labelling of two model β-glucosidases. The results indicate that the LDC approach can be adaptable for covalent proximity labelling of glycoside hydrolases. 相似文献
14.
Guoliang Li Cuihua Song Jinmao You Xiaolong Zhang Guang Chen 《Journal of the American Oil Chemists' Society》2012,89(4):585-595
Free fatty acids (FFA) are basic and indispensable components of medicinal plants. Many researches indicate that the efficacy
of medicinal plant requires the presence of FFA. In the present study, ultrasonic-assisted extraction of FFA from Swertia species was optimized by response surface methodology (RSM), ensuring the highest FFA recoveries. A novel pre-column fluorescence
labelling method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as a labelling reagent has been developed for highly sensitive and selective analysis of FFA by
HPLC with fluorescent detection (FLD) and online mass spectrometry identification. RSM was also employed to optimize the fluorescence
labelling of FFA. HPLC separation of 17 FFA derivatives was carried out on a reversed-phase Hypersil BDS C8 column (4.6 × 200 mm, 5 μm, Agilent Co.) with a gradient elution. The detection limits and quantifications were as low as
0.60 and 1.22 ng mL−1, respectively. FFA analysis from nine Swertia species was performed by the newly developed method, and the FFA composition of these Swertia samples was first reported. 相似文献
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Characterisation of Photoaffinity‐Based Chemical Probes by Fluorescence Imaging and Native‐State Mass Spectrometry 下载免费PDF全文
Kanae Teruya Dr. Gregory M. Rankin Dr. Panagiotis K. Chrysanthopoulos Prof. Kathryn F. Tonissen Prof. Sally‐Ann Poulsen 《Chembiochem : a European journal of chemical biology》2017,18(8):739-754
Chemical probes are small‐molecule reagents used by researchers for labelling and detection of biomolecules. We present the design, synthesis, and characterisation of a panel of 11 structurally diverse photoaffinity labelling (PAL) probes as research tools for labelling the model enzyme carbonic anhydrase (CA) in challenging environments, including in protein mixtures and cell lysates. We targeted the ubiquitous CA II as well as the two cancer‐associated CAs (CA IX and CA XII) that are of high priority as potential biomarkers of aggressive and/or multidrug‐resistant cancer. We utilise an atypical biophysical approach, native state mass spectrometry, to monitor the initial protein–probe binding and subsequent UV crosslinking efficiency of the protein:probe complex. This mass spectrometry methodology represents a new approach for chemical probe optimisation and development that might have broader applications to chemical probe characterisation beyond this study. This also represents one of the first studies, to the best of our knowledge, in which a comprehensive set of PAL probes has been used to establish the relationship between probe structure, noncovalent protein–probe binding, and covalent protein–probe crosslinking efficiency. Our results demonstrate the benefits of a comprehensive analysis of chemical probe structure–activity relationships to support the development of optimum chemical probes. 相似文献
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Recently, interdisciplinary research in cancer diagnosis and therapy has evolved to the point where nanotechnology particularly polymeric nanodelivery systems are utilized for theragnostic applications. Nanoscale are being trialed for specific targeted delivery of drugs, micelles, antibody, DNA, protein, etc. to cancer sites to improve the therapeutic efficacy due to improved distribution specificity, increased internalization, and intracellular drug delivery that minimize the side effects. Polymeric micelles have been subjected to extensive studies in the field of drug delivery, functioning as drug solubilizers and carriers. More recently, a micelle constructed as a hybrid from hydrophilic oligonucleotide and hydrophobic polymer has drawn close attention. Mostly used micelles are synthesized with polymer and have several physical properties, including molecular weight and copolymer block composition, which can be tailored to alter the vesicle structure. In this review, we focused on the different polymeric nanodelivery systems is association with different type of cancer therapeutics such as micelles, drug, aptamer, DNA, recombinant protein, miRNA, siRNA, small inhibitors, gene, antibody, proteins and some conjugating molecules that involved in cancer therapy have been discussed. 相似文献
18.
《Journal of Adhesion Science and Technology》2013,27(3):409-421
Machining performance is one of the most important features of waterborne biopolymer labelling adhesives. Various deformation processes on the labelling machine have been analysed in relation to the rheology characteristics of adhesive materials. It has been shown that satisfactory machining performance requires adhesive materials to have particular properties under both shear and extensional deformations. Flow behaviours of commercial labelling adhesive products were tested over a very wide range of shear rate. Time dependences of the apparent shear viscosity were examined at selected shear rates. An adhesive with a smooth flow behaviour and only slight time dependence tends to perform better on the labelling machine. Results found in this work can be used as guide for rheology assessment in the development of new waterborne biopolymer adhesives. 相似文献
19.
Rodica E. Ionescu Chantal Gondran Nicole Jaffrezic-Renault Claude Martelet 《Electrochimica acta》2010,55(21):6228-68
Impedance spectroscopy transduction combined with the immunosensor technology has been used for the determination of atrazine, a herbicide The sensor electrode was based on the immobilization of anti-atrazine antibody by affinity binding onto a polypyrrole film N-substituted by nitrilotriacetic acid NTA electrogenerated on a gold electrode. The poly NTA film was previously modified by the coordination of Cu2 ions by the chelating NTA centers. The anti-atrazine antibody Fab fragment K47 modified with histidine-tag was then anchored by affinity interactions between the histidine-tag and the coordinated Cu2. Cyclic voltammetry experiments confirm that the antibody immobilization and the resulting immunosensor were applied to the impedimetric detection of atrazine without reagent and labelling step. The immunoreaction of atrazine on the attached anti-atrazine antibody directly triggers an increase in the charge transfer resistance proportional to the atrazine concentration, allowing the detection of extremely low atrazine concentration, namely 10 pg mL−1. 相似文献
20.
B. A. Coles 《Journal of the American Oil Chemists' Society》1980,57(7):202-204
Protein may be determined using a pulsed nuclear magnetic reso-nance (NMR) spectrometer in conjunction with a relaxation reagent.
These reagents exhibit characteristic nuclear relaxation rates, controlled by a paramagnetic ingredient such as copper, and
these rates are altered if substances are added which can bind the copper. The method is fast and simple. Sample and reagent
are mixed, brought to a standard temperature and transferred to the spectrom-eter for measurement. Sample throughput can be
high since the time for the spectrometer reading is on the order of 30 sec. Compositions and methods of use are given for
2 copper-based reagents together with the results on a number of materials. The alkaline copper reagent gives a very similar
response to different types of protein and it appears suitable for meat, fish and for other protein materials low in carbohydrates.
It shows a response to carbohydrate which depends strongly on type. The acid copper reagent is intended for measurements of
vegetable or seed protein and similar applications where carbohydrate is present. This reagent does not respond to carbohydrates
tested; the response to protein depends on the type of protein. 相似文献