蛋清中溶菌酶的提取研究

以鸡蛋清为原料 ,控制温度 5℃ ,采用 72 4型树脂吸附 ;再用 φ(H2 SO4 ) =1%的稀硫酸分四次洗脱 ,用NaOH调pH =8 5～ 9 0 ,除去杂质 ;再用c(HCl) =3 0mol/L的盐酸调pH =3 5 ,加入w (NaCl) =5 %的溶液使溶菌酶析出 ,用无水丙酮 0℃干燥即得溶菌酶成品。     

用自动系统凯氏定氮仪测定生物制品中蛋白质含量

应用自动系统凯氏定氮仪测定人血白蛋白和人血丙种球蛋白的蛋白含量 ,重复性测定X % (n =10 )±SD分别为 2 0 45 %± 0 18%和 10 2 5 %± 0 12 % ;用硫酸铵、氨基乙酸和标准蛋白质控液作回收率试验R % (n =10 )±SD分别为 10 0 .2 4%± 0 5 7% ,99.89%± 0 43%和 10 0 5 0 %± 0 75 % ;用该法测定蛋白质含量与手工定氮法比较有良好相关性 ,总氮 (TN)相关系数r(n =2 6 )为 0 9999;非蛋白氮 (NPN)r(n =19)为 0 9946 ;蛋白氮 (PN)r(n =19)为 0 .9999。测定同批标准人血白蛋白 ,结果X % (n =2 0 )±SD为 5 5 3%± 0 14% ,与其参考标准值 5 5 4%± 0 12 %非常接近。因此 ,用该仪器法测定生物制品中的蛋白质含量准确可靠 ,且快速。     

猫眼草不同药用部位总黄酮含量测定

用60%乙醇超声提取猫眼草根、茎、叶不同部位中的总黄酮,并以芦丁为对照品,用紫外分光光度计在510 nm处测定不同药用部位中总黄酮的含量。结果表明,芦丁标准溶液浓度在12.5~50μg/mL与吸光度呈良好的线性关系,线性回归方程为Y=0.011 5X-0.023 5,r=0.999 4（n=5）,全草的平均回收率为99.60%,RSD为1.60%。测得猫眼草根、茎、叶中黄酮含量分别为20.30,16.32,23.53 mg/g,叶中的总黄酮含量比根、茎含量高。     

新鲜山矾花头香成分的研究

用 60H型硅胶吸收刚采摘的鲜山矾花的头香成分 ,用无水乙醚洗脱 ,高纯氮吹出乙醚浓缩 ,所得的鲜山矾花的头香成分浓缩液用气相色谱 -质谱 (GC -MS)法分离并分析鉴定其成分及质量分数 ,共鉴定出 2 2个化合物 ,其中所鉴定的鲜山矾花的头香成分的组分占挥发性成分总峰面积的 91 2 0 %。它们是 :w (双花醇 ) =2 5 0 1%、w(反式 氧化芳樟醇 ) =6 2 4%、w(顺式 氧化芳樟醇 ) =1 0 8%、w(L 芳樟醇 ) =18 98%、w (紫丁香醇 ) =2 3 7%、w(紫丁香醛 ) =0 5 6%、w(月桂醇 ) =0 2 5 %、w(氧化橙花醇 ) =3 0 9%、w(乙苯 ) =2 0 0 %、w(2 ,6 二甲基 3 ,7 辛二烯 2 ,6 二醇 ) =10 17%、w(3 ,4,5 三甲氧基甲苯 ) =7 3 5 %、w (4 甲基 2 ,6 二叔丁基 4 羟基 2 ,5 环己二烯 1 酮 ) =1 0 9%、w(2 ,6 二叔丁基对苯醌 ) =0 41%、w(2 ,6 二叔丁基对甲苯酚 ) =2 61%、w(3 ,5 二叔丁基 4 羟基苄醇 ) =0 3 9%。     

海南红厚壳中生物碱的提取与含量测定

以海南红厚壳枝、叶为材料,以氯仿作溶剂,微波法提取2次,根据酸性染料比色法原理,以盐酸小檗碱为对照品,溴甲酚绿为指示剂,在波长416 nm条件下,用紫外可见分光光度计测定其中的总生物碱的含量。标准方程式为:A=-0.007 58+0.0384 2C,相对标准偏差r=0.999 9(n=6)。测定结果为叶中总生物碱含量=0.172%,加标回收率=97.3%,RSD=1.64%(n=5);枝中含量=0.074 0%,加标回收率=95.3%,RSD=2.05%(n=5)。     

降解淀粉与乙烯基类单体接枝聚合物的合成及应用

适当降解的玉米淀粉与乙烯基类单体在引发剂作用下通过接枝聚合得到了改性淀粉鞣剂。当体系中w (降解淀粉 ) =10 %、w (乙烯基类单体 ) =2 0 %、引发剂质量为反应物质量的2 .0 %、反应温度为 5 0℃、反应时间为 1 5h时所得聚合物的接枝百分率为 87%、接枝效率为94%及接枝频率为 43。用其预鞣时可减少铬鞣剂用量 30 %～ 5 0 % ,铬鞣废液中铬含量降低到 ρ(Cr2 O3) =0 .2 6g/L ,用其复鞣时所得成革的选择填充性显著。成品革丰满柔软 ,粒面细腻。     

高效液相色谱法同时测定珍菊降压片中氢氯噻嗪和芦丁的含量

建立以高效液相色谱法测定珍菊降压片中氢氯噻嗪和芦丁含量的方法.用十八烷基硅烷键合硅胶为填充剂;以醋酸铵高氯酸钠溶液-乙腈(80:20)(用H3PO4调节pH值至2.5)为流动相;检测波长为260nm,进样量为20mL.氢氯噻嗪和芦丁的线性范围分别为3～600mg·mL-1(r=0.9995)、6～1000mg·mL-1(r=0.9999);氢氯噻嗪的平均回收率为100.1%(RSD=1.8%,n=5);芦丁的平均回收率为99.9%(RSD=1.4%,n=5).本方法简单、快速、准确,稳定,重现性好.     

盐酸赖氨酸及其注射液的HPLC测定

用 2 ,4 -二硝基氟苯为衍生化试剂将盐酸赖氨酸缩合成二硝基氟苯氨基酸 ,用反相 HPL C法测定盐酸赖氨酸及其注射液的含量。盐酸赖氨酸浓度在 1.92～ 9.6 0 μg· m L- 1范围内线性良好 ,相关系数为 0 .9992 ,测得原料药平均回收率为 99.2 % ,RSD为 0 .78% (n=5 ) ;注射液平均回收率为 99.0 % ,RSD为 0 .90 % (n=5 ) ,方法简便、准确     

过氧乙酸高效液相色谱法分析方法研究

试样用 pH =3～ 4的新蒸二次蒸馏水溶解 ,以 pH =3～ 4新蒸二次蒸馏水为流动相 ,用Nova-packC18柱分离 ,紫外检测器 ( 2 1 0nm)检测 ,外标法定量。本方法的相对标准偏差为 0 .5 % ,平均回收率为 99.4% ,完成一次分析只用 5分钟。     

伯胺N_(1923)对螺旋霉素和红霉素的萃取性能研究(1)

研究了伯胺N192 3 萃取剂对螺旋霉素和红霉素的萃取性能 ,实验结果表明它具有萃取率高、适用 pH范围较大、不乳化、易分相、易反萃、用量小且可多次循环使用等优点 ,显示出了作为工业萃取剂的良好特性。适宜的萃取条件分别为对螺旋霉素用 5 0 %N192 3,pH=9.56,O/A =1∶1(V/V) ,萃取率为 90 8% ;萃取红霉素用 7 5%N192 3,pH =9.50 ,O/A =1∶1,萃取率为 92 %。     

高效液相测定沙棘果皮中熊果酸和齐墩果酸的含量

建立沙棘果皮中齐墩果酸、熊果酸的含量测定方法。采用高效液相色谱法,Nova-Pack型C18柱,乙腈-甲醇-水-醋酸铵（65∶20∶15∶0.5）为流动相,检测波长为210nm;流速0.6mL/min。熊果酸回归方程为y=74.0x＋9.12,r=0.9995,线性范围0.11～10.64μg/mL,齐墩果酸回归方程为y=61.6x＋7.40,r=0.9992,线性范围0.12～12.36μg/mL。沙棘果皮中熊果酸和齐墩果酸的平均含量为1.187mg/g和0.352mg/g,平均回收率为100.3%、100.5%（n=3）,RSD分别为1.47%、1.50%（n=3）。该方法可同时测定沙棘果皮中齐墩果酸和熊果酸的含量,可用于药材质量控制。     

离子液体中结晶分离熊果酸和齐墩果酸研究

为拓展结晶溶剂的范围和提高结晶分离熊果酸和齐墩果酸的性能,引入离子液体作为结晶溶剂。测定了熊果酸和齐墩果酸在六种离子液体+乙醇溶液中的溶解度数据,根据该数据,选取1-辛基-3-甲基咪唑六氟磷酸+乙醇混合溶液作为溶剂对熊果酸和齐墩果酸进行了结晶分离,并采用单因素实验对结晶工艺进行了初步优化。结果表明：在1-辛基-3-甲基咪唑六氟磷酸质量分数5%,熊果酸和齐墩果酸质量比1.5∶1,结晶温度30℃,结晶时间14 h的条件下,结晶产物中齐墩果酸的质量分数可以达到85%左右。     

细基丸叶和茎总三萜含量测定

测定细基丸叶和茎中总三萜的含量。以乌苏酸为对照品,以质量分数5%香草醛-冰醋酸和高氯酸系统为显色剂,采用紫外分光光度法在546 nm波长处测定样品的吸光度。乌苏酸对照品在20～100μg/mL范围内呈良好线性关系,回归方程为Abs=0.1548C+0.0286,r=0.9993。细基丸叶和茎中总三萜的含量分别为1.684%和0.5439%.本法操作简便,结果稳定,重复性好,易于推广。     

Microwave-Assisted Extraction of Oleanolic Acid and Ursolic Acid from Ligustrum lucidum Ait

Oleanolic acid and ursolic acid are the main active components in fruit of Ligustrum lucidum Ait, and possess anticancer, antimutagenic, anti-inflammatory, antioxidative and antiprotozoal activities. In this study, microwave-assisted extraction of oleanolic acid and ursolic acid from Ligustrum lucidum was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, microwave power, extraction temperature and microwave time, on the extraction efficiencies of oleanolic acid and ursolic acid from Ligustrum lucidum were evaluated. The influence of experimental parameters on the extraction efficiency of ursolic acid was more significant than that of oleanolic acid (p < 0.05). The optimal extraction conditions were 80% ethanol aqueous solution, the ratio of material to liquid was 1:15, and extraction for 30 min at 70 °C under microwave irradiation of 500 W. Under optimal conditions, the yields of oleanolic acid and ursolic acid were 4.4 ± 0.20 mg/g and 5.8 ± 0.15 mg/g, respectively. The results obtained are helpful for the full utilization of Ligustrum lucidum, which also indicated that microwave-assisted extraction is a very useful method for extraction of oleanolic acid and ursolic acid from plant materials.     

乌索酸的生产工艺

Production of ursolic acid, the anti-hepatitis effective composition from natural plant, Sambucus chinensis Lindl. was carried out and the scale-up preparation technology was studied. Extraction of the herb Sambucus chinensis Lindl. was extracted in reflux with 7 volume times of refluxing ethanol at 80℃ for 1 h for two times. The extract was purified with a seh-made specific impurity remover ＂YCXY-1＂ to yield ursolic acid of high purity. A new ＂Extraction/Gelation＂ technology for the production of ursolic acid was developed. The specific impurity remover ＂YCXY-1＂ showed high effectiveness in purification. The purity of the mass-produced ursolie acid was up to 99.8%. The chemical structure of the product was confirmed by the physicochemical constants and spectroscopic identification. The production of high-purity ursolic acid was optimized with natural plant as raw material and with only ethanol as extracting solvent. The difficulties such as isolation and impurity removal were addressed effectively. The novel technology is reasonable, convenient, practical, low-cost, high-yield, suitable for mass production.     

高效液相色谱法同时测定云南白花木瓜不同部位中5个成分的含量

建立了一种采用高效液相色谱(HPLC)法同时测定白花木瓜中绿原酸、芦丁、金丝桃苷、齐墩果酸、熊果酸的质量浓度的方法,比较了果皮、果肉中绿原酸、芦丁、金丝桃苷、齐墩果酸、熊果酸质量浓度的差异.结果显示,在以A相(甲醇)和B相(体积分数为16％乙腈,体积分数为0.3％磷酸水溶液)为流动相的梯度洗脱条件下,绿原酸、芦丁、金丝...     

熊果酸香皂的制备及其生物活性研究

以棕榈油皂粒为皂基制备熊果酸香皂,按照国家标准方法对香皂中游离苛性碱、水分和挥发物含量进行检测,采用平板计数法和动物实验对该香皂的体外抑菌作用和安全性进行研究。结果表明,熊果酸香皂对白色假丝酵母、肺炎克雷伯氏菌、伤寒沙门氏菌、铜绿假单胞菌、MRSA1和MRSA2均具有明显抑菌作用,在熊果酸质量分数为7.5%时抑菌效果最为显著,动物实验表明香皂对小鼠皮肤和眼睛黏膜无不良反应和毒副作用,长期使用不会对皮肤产生刺激性。     

苦丁茶中熊果酸的提取及含量测定研究

对苦丁茶的提取方法进行考查,并对各种苦丁茶中的熊果酸进行定性鉴别和含量测定。方法考查结果表明：以乙醚为溶剂,索氏提取3．5h为宜,此法重现性和回收率良好。定性鉴别和含量测定结果表明：冬青科苦丁茶熊果酸含量较高,而木樨科苦丁茶不含熊果酸,且熊果酸的含量随贮存时间的延长而降低。     

Ultrasonic assisted extraction of ursolic acid from apple pomace: A novel and facile technique

Substantial amount of apple pomace (AP) is produced in apple processing industries and is often discarded as waste which becomes a source of polluting the environment. However, the by-product constitutes a promising bioresource of ursolic acid with various biological activities. Therefore, this particular research study was aimed to develop a novel and facile method to separate ursolic acid from AP. First, AP was extracted with ultrasound assistance using methanol as extraction solvent, 2.86% with 10:1 (mL g^?1) of liquid–solid ratio, 300 W of ultrasonic power, 50°C of extraction temperature, and 90 min of ultrasonic time. Meanwhile, the disruption effect of ultrasound on AP was observed by scanning electron microscope. Second, a novel and facile method was applied to separation of ursolic acid, then a perfect colorless needle crystal of ursolic acid was obtained with the yield of about 1.34% of dry AP.     

微波法合成熊果酸衍生物

以熊果酸为原料,分别通过常规方法及微波法合成3-O-乙酰基熊果酸和熊果酸丁酯,并经核磁共振氢谱确证其化学结构。结果表明,微波法大大缩短了反应时间,提高了收率。