偶氮胭脂红G结合共振光散射法测定人血清样品中的总蛋白

研究了在pH为2.5的BR缓冲酸性介质中,偶氮胭脂红G与牛血清白蛋白(BSA)、人血清白蛋白(HSA)、鸡蛋白蛋白(OVA)及免疫球蛋白(γ-IgG)等蛋白质作用产生共振光散射(RLS)增强信号,最大散射峰位于594nm处。在最佳实验条件下,增强RLS强度在594 nm处与蛋白质浓度呈线性关系,检出限在0.025~0.406μg/mL之间,生物体液中大部分常见物质均不产生干扰。该法应用于人血清样品测定,测定结果与考马斯亮蓝法一致。     

曙红Y共振光散射法测定水中十六烷基三甲基溴化铵

利用共振光散射(RLS)技术,通过曙红Y与阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)缔合产生强烈共振光散射增强效应,建立了一种测定阳离子表面活性剂CTAB的方法.实验结果表明,在pH=4.96的柠檬酸钠-盐酸缓冲介质中,采用吐温80作为曙红Y -CTAB体系的稳定剂,痕量CTAB的加入导致曙红Y在440～ 600 nm波长内的共振光强度增加,最大RLS峰位于538 nm处,其强度与CTAB的质量浓度在0～40 mg·L-1内成正比,检出限为0.012 mg·L-1.方法具有较高的灵敏度和良好的选择性,可用于水样中CTAB的测定.     

吡咯红Y-SDS-蛋白质体系的共振光散射光谱研究

在硫酸存在下,微量蛋白质的加入可使吡咯红Y-SDS体系弱的共振光散射信号得到极大的增强。在λ=364 nm处,光散射强度最大,并且光散射增强强度与蛋白质的质量浓度在一定范围内呈线性关系,由此建立了一种测定蛋白质的新的分析方法。对牛血清白蛋白(BSA)和人血清白蛋白(HSA)进行测定,其线性范围分别为0.15~3.6μg/mL和0.06~4.8μg/mL,检测限分别为21.0 ng/mL和12.0 ng/mL。该方法用于人血清样中蛋白总含量的测定,结果令人满意。     

邻苯二酚紫共振光散射光谱法测定蛋白质的研究

基于人血清白蛋白(HSA)对邻苯二酚紫的共振光散射的增强效应，拟定了一种测定HSA的新的共振光散射体系。在pH2．56的Brltton-Robinson缓冲溶液中，邻苯二酚紫与HSA结合导致共振光散射增强，在λ=515．0nm处共振光散射有最大的强度，并且共振光散射强度与HSA的浓度在0．0～20．0μg／mL范围内有良好的线性关系，检测限可达0．18μg／mL。该方法简便、快速，用于人体血清样品的测定并与经典的考马斯亮蓝法比较，结果满意。     

锌(Ⅱ) - 2,2''-联吡啶络合吸附波法测定血清白蛋白

在pH值6.3的HAc-NaAc介质中,血清白蛋白使锌(Ⅱ) - 2,2′-联吡啶络合物在-1.20 V(vs.SCE)处的络合吸附波还原峰电流降低,峰电流降低值与加入的牛血清白蛋白(BSA)或人血清白蛋白(HSA)的浓度在一定范围内呈线性关系.BSA和HSA的线性范围分别为0.5～40.0 mg·L-1、0.5～50.0 mg·L-1,检出限均为0.2 mg·L-1.应用该法测定了人血清样品中总蛋白含量,结果令人满意.     

全氟辛基季胺碘化物与DNA作用的共振光散射光谱分析

研究了全氟辛基季胺碘化物（FC-134）与脱氧核糖核酸（DNA）作用的共振光散射光谱。FC-134本身共振光散射强度较弱,在加入DNA后,共振光强度明显增强,其强度与DNA浓度成正比,并且在pH3．5时,DNA与FC．134在370nm处共振光散射强度达到最大,且保持稳定。ctDNA的线性范围为0．2～1．0mg·L^-1,检测限为0．017mg·L^-1。该法用于合成样品中DNA的测定,测定结果较好。     

DBC-偶氮羧与铝(Ⅲ)显色反应的研究及应用

在pH 6.0的六次甲基四胺-HCl缓冲溶液中,铝(Ⅲ)与DBC-偶氮羧形成蓝色络合物,最大吸收波长为622 nm,考察了实验条件对显色反应的影响,铝(Ⅲ)浓度在0～15 μg·(25 mL)-1范围内符合朗伯比尔定律.在乙二醇和硫脲-亚硫酸钠等掩蔽剂存在下, 用于镁合金中铝的测定,结果令人满意.     

对二甲胺基亚苄罗丹宁与人血清白蛋白的显色反应研究

在pH8.0的磷酸盐缓冲溶液中,对二甲胺基亚苄罗丹宁(pDBR)与人血清白蛋白(HSA)形成橙红色复合物,λmax在480nm左右,ε为9.8×104L·mol-1·cm-1。蛋白质浓度在7.5～143mg/L范围内服从Beer′s定律。所提出的方法可直接用于测定血清中蛋白质的含量。     

偶氮硝羧与血清蛋白质反应的分光光度研究

在pH3.0的BrittonRobinson缓冲介质中,红色的偶氮硝羧与蛋白质形成蓝紫色复合物[1],其最大吸收波长约为680nm,比试剂本身红移140nm,表观摩尔吸光系数达2.60×105Lmol·cm(牛血清白蛋白),线性范围为20～140μgmL。实验制定的几种蛋白质的标准工作曲线,其线性范围与牛血清白蛋白一致,灵敏度高。用于人血清样品测定,方法可靠。     

阳离子型Gemini表面活性剂共振光散射法测定DNA及相互作用机理的研究

在酸性介质(pH=3.51)中,脱氧核糖核酸(DNA)能显著地增强阳离子型Gemini表面活性剂(G14-4-14)的共振光散射信号,据此提出了G14-4-14共振光散射法测定DNA的新方法,并对介质酸度、离子强度、G14-4-14浓度及共存物质干扰等实验条件进行了优化。在最佳条件下绘制了工作曲线,该方法线性范围为0～2760μg.L-1,检出限为7.79μg.L-1,用于合成样品的测定,结果令人满意。以芘(pyrene)为荧光探针,应用稳态荧光法探讨了G14-4-14与DNA的相互作用,并运用红外光谱及共振光散射光谱初步探讨了G14-4-14与DNA相互作用的机理。     

Insect antifeedant properties of anthranoids from the genusVismia

Vismiones and ferruginins, representatives of a new class of lypophilic anthranoids from the genusVismia were found to inhibit feeding in larvae of species ofSpodoptera, Heliothis, and inLocusta migratoria.     

Methyl 2,4,6-decatrienoates Attract Stink Bugs and Tachinid Parasitoids

Halyomorpha halys (Stål) (Pentatomidae), called the brown marmorated stink bug (BMSB), is a newly invasive species in the eastern USA that is rapidly spreading from the original point of establishment in Allentown, PA. In its native range, the BMSB is reportedly attracted to methyl (E,E,Z)-2,4,6-decatrienoate, the male-produced pheromone of another pentatomid common in eastern Asia, Plautia stali Scott. In North America, Thyanta spp. are the only pentatomids known to produce methyl 2,4,6-decatrienoate [the (E,Z,Z)-isomer] as part of their pheromones. Methyl 2,4,6-decatrienoates were field-tested in Maryland to monitor the spread of the BMSB and to explore the possibility that Thyanta spp. are an alternate host for parasitic tachinid flies that use stink bug pheromones as host-finding kairomones. Here we report the first captures of adult and nymph BMSBs in traps baited with methyl (E,E,Z)-2,4,6-decatrienoate in central Maryland and present data verifying that the tachinid, Euclytia flava (Townsend), exploits methyl (E,Z,Z)-2,4,6-decatrienoate as a kairomone. We also report the unexpected finding that various isomers of methyl 2,4,6-decatrienoate attract Acrosternum hilare (Say), although this bug apparently does not produce methyl decatrienoates. Other stink bugs and tachinids native to North America were also attracted to methyl 2,4,6-decatrienoates. These data indicate there are Heteroptera in North America in addition to Thyanta spp. that probably use methyl 2,4,6-decatrienoates as pheromones. The evidence that some pentatomids exploit the pheromones of other true bugs as kairomones to find food or to congregate as a passive defense against tachinid parasitism is discussed.     

2005～2006年国外塑料工业进展

收集了2005年7月～2006年6月国外塑料工业的相关资料，介绍了2005—2006年国外塑料工业的发展情况。提供了世界塑料产量、消费量及全球各类树脂生产量以及各国塑料制品的进出口情况。作为对比，介绍了中国塑料的生产情况。按通用热塑性树脂（聚乙烯、聚丙烯、聚苯乙烯、聚氯乙烯、ABS树脂）、工程塑料（聚酰胺、聚碳酸酯、聚甲醛、热塑性聚酯、聚苯醚）、通用热固性树脂（酚醛、聚氨酯、不饱和树脂、环氧树脂）、特种工程塑料（聚苯硫醚、液晶聚合物、聚醚醚酮）的品种顺序，对树脂的产量、消费量、供需状况及合成工艺、产品开发、树脂品种的延伸及应用的扩展作了详细的介绍。     

2007～2008年世界塑料工业进展

收集了2007年7月～2008年6月世界塑料工业的相关资料,介绍了2007～2008年国外塑料工业的发展情况,提供了世界塑料产量、消费量及全球各类树脂的需求量及产能情况.按通用热塑性树脂(聚乙烯、聚丙烯、聚苯乙烯、聚氯乙烯、ABS树脂)、工程塑料(尼龙、聚碳酸酯、聚甲醛、热塑性聚酯、聚苯醚)、特种工程塑料(聚苯·硫醚、液晶聚合物、聚醚醚酮)、通用热固性树脂(酚醛、聚氨酯、不饱和聚酯树脂、环氧树脂)不同品种的顺序,对树脂的产量、消费量、供需状况及合成工艺、产品应用开发、树脂品种的延伸及应用的进一步扩展等技术作了详细介绍.     

Inorganic/organic nanocomposite coatings: The next step in coating performance

Inorganic/organic hybrid materials have considerable promise and are beginning to become a major area of research for many coating usages, including abrasion and corrosion resistance. Our primary approach is to prepare the inorganic phase in situ within the film formation process of the organic phase. The inorganic phase is introduced via sol-gel chemistry into a thermosetting organic phase. By this method, the size, periodicity, spatial positioning, and density of the inorganic phase can be controlled. An important aspect of the inorganic/organic hybrid materials is the coupling agent. The initial task of the coupling agent is to provide uniform mixing of the oligomeric organic phase with the sol-gel precursors, which are otherwise immiscible. UV-curable inorganic/organic hybrid systems have the advantages of a rapid cure and the ability to be used on heat sensitive substrates such as molded plastics. Also, it is possible to have better control of the growth of the inorganic phase using UV curing. It is our ultimate goal to completely separate the curing of inorganic and organic phases to gain complete control over the morphology, and hence optimization of “all” the coating properties. Thus far, it has been found that concomitant UV curing of the inorganic and organic phases using titanium sol-gel precursors afforded nanocomposite coatings which completely block the substrate from UV light while maintaining a transparent to visible light. Also, it has been found that the morphology of the inorganic phase is highly dependent on the concentration and reactivity of the coupling agent. Presented at the 82nd Annual Meeting of the Federation of Societies for Coatings Technology, October 27–29, 2004, in Chicago, IL.     

Ethanol and (−)-α-Pinene: Attractant Kairomones for Some Large Wood-Boring Beetles in Southeastern USA

Ethanol and α-pinene were tested as attractants for large wood-boring pine beetles in Alabama, Florida, Georgia, North Carolina, and South Carolina in 2002–2004. Multiple-funnel traps baited with (−)-α-pinene (released at about 2 g/d at 25–28°C) were attractive to the following Cerambycidae: Acanthocinus nodosus, A. obsoletus, Arhopalus rusticus nubilus, Asemum striatum, Monochamus titillator, Prionus pocularis, Xylotrechus integer, and X. sagittatus sagittatus. Buprestis lineata (Buprestidae), Alaus myops (Elateridae), and Hylobius pales and Pachylobius picivorus (Curculionidae) were also attracted to traps baited with (−)-α-pinene. In many locations, ethanol synergized attraction of the cerambycids Acanthocinus nodosus, A. obsoletus, Arhopalus r. nubilus, Monochamus titillator, and Xylotrechus s. sagittatus (but not Asemum striatum, Prionus pocularis, or Xylotrechus integer) to traps baited with (−)-α-pinene. Similarly, attraction of Alaus myops, Hylobius pales, and Pachylobius picivorus (but not Buprestis lineata) to traps baited with (−)-α-pinene was synergized by ethanol. These results provide support for the use of traps baited with ethanol and (−)-α-pinene to detect and monitor common large wood-boring beetles from the southeastern region of the USA at ports-of-entry in other countries, as well as forested areas in the USA.