一种新型农药浓度荧光测量系统的研究

根据声光可调滤波器（Acousto-Optic Tunable Filters，AOTF）的分光原理研制了一种新型的农药浓度荧光俭测系统，系统以高压短弧氙灯为激发光源，以AOTF为分光元件，利用光纤探测传输荧光，采用数据采集卡实现数据接收和A／D转换，由微机进行数据存储和显示，并且利用该系统实现了对西维因农药浓度的荧光持性的测量。结果表明：系统测量光谱范围为230nm～750nm，光谱分辨率为10nm，在激发波K319nm、荧光波长650nm、西维因溶液浓度为0．0～120．0μg／L时系统具有较好的线性关系，线性相关系数r为0．9991，该系统达到了荧光检测的指标。     

基于荧光机理的土壤农药浓度光纤检测系统

根据有机物分子受激发荧光的基本原理,分析了荧光法检测农药的可行性.基于光纤传感技术、荧光分析技术提出了一种能够检测土壤中有机农药浓度的光纤式荧光测量系统.系统以脉冲氙灯为激发光源,以特制的光纤式锥形探头探测荧光,以小型平场光谱仪实现荧光分光,以高速数据采集模块实现荧光信号的采集转换.该系统一次曝光即可获得农药的荧光光谱.利用该系统实现了不同浓度的西维因在土壤中荧光光谱实验,考察了系统的工作曲线和最低检测限.实验结果表明,系统能够获取分辨率较高的荧光光谱;在0.005 mg/kg~0.1 mg/kg范围内荧光强度和浓度基本呈线性关系;系统的最低检出限(LOD)可达0.005 mg/kg,相对标准偏差(RSD)≤3%.该系统能够满足土壤中农药浓度检测的需要.     

基于AOTF的在线红外光谱测量系统

介绍了一种新型的红外光谱测量系统，它由准直发射系统和聚焦接收系统两大部分组成，以声光可调谐滤光器（AOTF）为色散元件，连续改变AOTF的驱动频率就能实现快速波长扫描，达到实时测量目的。该系统具有结构简单、测量快速等特点，可用于在线有害气体检测和红外光谱分析。实验表明，系统光谱测量范围2．5－5μm，光谱分辨力为10nm。     

基于CCD器件的苯甲酰脲类农药荧光检测系统

根据苯甲酰脲类农药在紫外光的照射下能够发出荧光的机理,设计了一种基于CCD(电荷耦合器件)的检测该类有机农药残留的光纤荧光测量系统.该系统以脉冲氙灯为激发光源,利用光纤探测和传输荧光,采用线阵CCD代替传统的光电倍增管作为荧光信号的光电检测元件,同时配备A/D高速数据采集卡,实现了单片机控制下荧光信号的光电转换以及数据采集,进而实现了对卡死克和盖虫散(氟铃脲)农药浓度的测量.实验结果表明,在激发波长分别为290nm和345nm时,卡死克和盖虫散的荧光强度分别在360nm和418nm处达到最大,最低检出限分别为12 μg/L和20 μg/L.在25～1 000μg/L范围内,荧光强度和溶液浓度基本呈线性关系.该测量系统灵敏度高,线性范围宽,可以满足荧光检测的要求.     

红外可调声光滤光器的设计和特性分析

根据声光互作用原理，以TeO2晶体为互作用介质，选取互作用长度为28mm，设计制成近红外声光可调滤光器（AOTF），其衍射波长与超声频率存在对应关系。测试结果表明，该器件的工作波长范围为0.9-2.6μm，驱动频率为40－120MHz，光谱分辨力为7.268nm，6.957nm（对应波长分别为1.5μm，2.4μm）。     

荧光分光光度法测定生活用纸制品中的荧光增白剂

通过对三种常用二苯乙烯型荧光增白剂VBL、BA、CXT荧光光谱和标准曲线的研究,建立了以VBL为标准物质检测生活用纸中荧光增白剂的定量方法.该方法激发波长为345 nm,发射波长为430 nm;定量标准曲线为γ(μg/mL)=0.0017x-0.0187,在O～1.0μg/mL范围内线性相关性R2=0.9992;方法检出限4μg/g,定量限10μg/g,平均加标回收率为95.3%,可满足检测要求.对pH和实验用水纯度对VBL稳定性的影响进行了初步探讨,发现实验中应使用纯度高的水和在中性或弱碱性的pH条件下,结果比较稳定.     

甲醇溶液的荧光光谱特性

应用SP-2558多功能光谱测量系统,由Xe灯通过激发光谱仪获得不同波长的紫外光,对不同浓度甲醇溶液在不同波长紫外光激励下产生荧光光谱的特性进行了实验研究。结果表明,甲醇溶液在波长220nm左右的紫外光激励下能产生较强的荧光。荧光峰是310nm至370nm范围的宽谱峰;荧光峰值波长在337nm附近,并随激励光波长增大而产生红移;在同一紫外光激励下,荧光强度随甲醇溶液浓度的增大发生先增强再减弱的变化。根据分子光谱理论,经分析提出,该荧光是由甲醇分子中-OH基团的孤对电子跃迁产生的,荧光光谱的上述特性由电子的跃迁情况决定。研究结果为甲醇作为常用溶剂和重要有机化工原料的应用、检测提供依据。     

用三维荧光技术检测氨基甲酸脂类农药残留研究

利用三维荧光技术,对西维因、灭多威、苯菌灵等三种常用氨基甲酸脂类农药的荧光特性进行了研究,分别绘制了三种农药溶液与混合溶液的三维总体积光谱图与等高线光谱图,证明了三维荧光光谱法检测农药残留的可行性,为农药残留的检测提供了理论依据和有效手段。     

一种潜在的激光制冷介质-罗丹明B掺杂PMMA

报道了罗丹明B掺杂PMMA材料制作过程及材料吸收和荧光光谱实验。吸收光谱表明，PMMA样品从紫外到近红外范围存在较小的吸收。罗丹明B／PMMA样品吸收光谱主要反映罗丹明B的吸收特征，其吸收峰中心波长位于550nm。荧光光谱显示，以630nm激发，反斯托克斯荧光峰位于595nm，能量差为0．11eV。该材料辐射反斯托克斯荧光，可用于激光制冷领域的研究。     

土壤中蒽的激光诱导荧光实验研究

常规利用液相色谱法检测土壤中的多环芳烃污染需要复杂的萃取过程,采用激光诱导荧光法直接检测土壤中的多环芳烃,有利于实现对土壤污染的监控.文章以多环芳烃中的蒽为研究对象,根据蒽的荧光特性,设计了实验光路和样品台,采用Nd:YAG激光器355 nm波长直接激发蒽和土壤混合样本的荧光,利用光纤光谱仪记录荧光光谱,实验发现在37...     

Highly selective and sensitive magnetic silica nanoparticles based fluorescent sensor for detection of Zn ions

In this work, quinoline group modified multifunctional silica nanoparticles having high magnetization and excellent Zn²⁺ selectivity have been successfully prepared. These multifunctional nanoparticles were characterized by high resolution transmission electron microscopy (HRTEM), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), X-ray powder diffraction (XRD) and vibrating sample magnetometer (VSM). The characterization data indicated that the organic ligand was successfully grafted on the surface of the magnetic silica nanoparticles. The fluorescent properties of the nanosensor were characterized and employed to detect Zn²⁺ with excellent selectivity and sensitivity (0.1 μM) toward Zn²⁺ over other cations even in trace amount.     

紧凑型荧光灯阴极电子发射材料的研究

本文通过紧凑型荧光灯阴极电子发射材料的研究，提出了改进紧凑型荧光灯阴极电子发射材料的建议，即将传统的用于直管形荧光灯的三元碳酸盐中的碳酸钡、碳酸锶和碳酸钙的比例由５６：３８：６调整为６１：２４：１５，并采用共结晶的方法制备细颗粒的三元碳酴盐，在发射性能方面取得了较好的结果．     

聚乙烯基吡咯烷酮对荧光增白剂增白效果的影响

研究了聚 Ｎ乙烯基吡咯烷酮（ 以下简称 Ｐ Ｖ Ｐ）／ 荧光增白剂溶液的荧光光谱特征及 Ｐ Ｖ Ｐ 对荧光增白剂增白效果的影响。     

Synthesis of fluorescence active pyridinedicarbonitriles and studying their application in functional paper

The present study deals with synthesis of novel 6-alkoxy-2-amino-3,5-pyridinedicarbonitrile derivatives, as a safety marker. The fluorescence properties of the synthesized heterocyclic compounds were determined. The fluorescence quantum yield (?_s) of these active compounds, relative to the fluorescence quantum yield of quinine sulfate, is reached at ~ 0.83. Application of representative synthesized examples on cotton linters and/or bagasse-based paper sheets was adopted in the present study, in an attempt to obtain safety paper from non-wood fibrous pulps. The quality of the obtained safety paper was evaluated based on both fluorescence impact and strength properties. The successful application of these fluorescent active compounds in the production of functionalized paper sheets, from bagasse pulp- cotton linter blend is demonstrated.     

Size-dependent self-assembly of submicron/nano beads-protein conjugates for construction of a protein nanoarray

A protein nanoarray is created when submicro and nano beads, varying in their size and each conjugated with different proteins, self-assemble to specific locations depending on the diameter matching the surface electron beam patterns created. Protein binding is confirmed from the fluorescence attenuation of the beads upon antigen–antibody binding on the bead surface. This method, called size-dependent self-assembly, allows control of the location of each type of bead, and thus, control of the location of multiple proteins. It provides fast multi-component patterning with a high binding resolution, which can be detected using a fluorescent light microscope. This method is developed to be a simple stand-alone tool for analysis of protein interactions. In addition, it has the potential to be used in conjunction with other protein analysis methods, such as enzyme-linked immunosorbent assay (ELISA) and atomic force microscopy (AFM).     

2–3 μm emission and fluorescent decaying behavior in Ho3+-doped tellurium germanate glass

In this work, we report the 2.05 μm emission and ∼3 μm broadband spectra of Ho₂O₃-doped 33GeO₂–30TeO₂–27PbO–10CaO (in mol%) glass under 640 nm laser excitation. Clear emission spectra due to the ⁵I₇–⁵I₈ transition and the ⁵I₆–⁵I₇ transition in Ho³⁺ are observed. The 2.05 μm emission intensity and the full width at half maximum (FWHM) of the ∼3 μm broadband depend on the Ho concentration. The peak stimulated emission cross-section of Ho³⁺ is 6.57 × 10⁻²¹ cm² at 2.05 μm, as calculated by the McCumber theory. The emission spectra are recorded and the maximum emission intensity at 2.05 μm is obtained at a doping level of 0.5 mol% Ho₂O₃ in the glass. A broad and flat emission band from 2700 nm to 3050 nm is observed in 2 mol% Ho₂O₃-doped tellurium germanate glass. The lifetime of the ⁵I₇ state decreases with the increase in Ho³⁺ concentration due to non-radiative relaxation processes. An energy transfer coefficient of 271.88 mol⁻¹ s⁻¹ is obtained.     

使用光纤实现荧光定量PCR检测

阐述了光纤在荧光定量PCR检测中的应用特点和荧光产生机理,给出了光纤耦合效率的计算公式。介绍了试验装置的构成和工作原理,列举了限制荧光检测的实际问题并提供了消除模块背景的方法。试剂检测的结果证实了使用光纤的检测系统具有很高的检测分辨率和8个数量级以上的动态线性范围,完全满足荧光定量PCR检测的要求。     

Dual detection biosensor based on porous silicon substrate

Due to the high surface-to-volume ratio (hundreds of m²/cm³) porous silicon became during the last years a good candidate material as substrate for biosensor application. Moreover, the versatility of surface chemistry allows different functionalization approaches and large number of molecules to be captured on well-defined areas. This paper reports a dual detection method for protein recognition processes developed on different nanostructured porous silicon (PS) substrates, based on using two complementary spectroscopic techniques: fluorescence and electrochemical impedance. The structures were tested for biomolecular recognition – biotin–strepavidin couples – in order to achieve an optimum surface for protein's immobilizations. Comparative analyses of the attachment degree and preservation of the biomolecules activity on the porous silicon surfaces and silicon slides are also described.     

混浊介质荧光显微成像的层析能力研究

采用蒙特卡罗方法给出了混浊介质显微成像中,共焦荧光、双光子激发荧光的空间分布。该分布表明双光子激发荧光显微成像具有内存的轴向层析能力,而共焦荧光成像的轴向层析能力依赖于共焦针孔的采用。进一步的研究则表明共焦针孔对双光子激发显微成像也同样具有提高层析能力的特点。