共查询到19条相似文献,搜索用时 203 毫秒
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细胞阵列芯片在细胞生物学研究、药物筛选等生物医疗领域有着广阔的应用前景.针对芯片上细胞固定时需要化学修饰的限制,提出了仅通过表面结构实现细胞固定的方案.基于MEMS技术,采用DRlE在硅片上得到超疏水的微纳结构,通过简单的高温氧化工艺实现超亲水性的SiO2 结构表面;再通过控制BOE腐蚀时间得到不同形貌的基底表面.计算了基底表面上的循环肿瘤细胞的黏附效率,并在SEM下观察了细胞与基底的相互作用行为,探讨了粗糙表面影响细胞黏附的原因.实验结果表明:采用DRlE和高温氧化制备的微纳SiO2 基底表面能极大促进细胞黏附,可应用于细胞阵列芯片设计中. 相似文献
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为了弄清阳极氧化制备TiO2纳米管阵列的影响因素及形成机理,研究了HF浓度W(HF)=0.1~1.0%,电压(5V~30V),时间(20min~120min)对阳极氧化TiO2纳米管阵列形貌的影响,用X射线衍射(XRD)和扫描电子显微镜(SEM)对其结构进行表征,并通过电流时间变化曲线探讨了TiO2纳米管阵列的形成机理.结果表明,氧化电压、电解液浓度、氧化时间是影响TiO2纳米管阵列形貌的主要因素,TiO2为不定形结构.只要延长时间,在低浓度W(HF)=0.1%HF溶液中也能得到TiO2纳米管阵列.阳极氧化的整个过程分为3个阶段,纳米管阵列的形成是场增强氧化、场增强氧化膜溶解和HF化学溶解共同作用并达到动态平衡的结果. 相似文献
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介绍一种硅纳米线制作方法.在SOI顶层硅上制作硅纳米梁,通过离子注入形成pnp结构,利用新发现的没有特殊光照时BOE溶液腐蚀pn结n型区域现象,结合BOE溶液氧化硅腐蚀,实现硅纳米线制作.制作完全采用传统MEMS工艺,具有工艺简单,成本低,可控,可靠性好,可批量制作等优点.利用该方法制作出了厚50 nm,宽100 nm的单晶硅纳米线,制作的纳米线可用于一维纳米结构电学性能研究、谐振器研究等. 相似文献
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为提高光刻仿真效率,通过对光刻原理进行研究,提出了2种多边形处理算法,将掩模上的多边形图案进行切分优化,将其划分成若干矩形或三角形。在Linux环境下应用C语言设计出一个完整的光刻仿真系统,设计的具体光学参数为:光源波长为193nm,数值孔径为0.3~0.8,部分相干系数可调范围为0.2~0.8,可一次性仿真1μm×1μm到10μm×10μm范围内的45 nm~0.18μm工艺的复杂版图,并通过多次实验进行验证。实验结果表明:原版图图像的边缘细节得到保留,且该算法有效地减少了光刻模拟的计算复杂度与计算时间,整体效率提升20%以上,为当前智能传感器系统芯片的制造节省了宝贵时间。 相似文献
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采用印刷电路板技术加工出芯片模具,以聚二甲基硅氧烷(PDMS)为材料制作出微流控芯片。该芯片由基片和盖片组成,微流控沟道位于基片上,深度和宽度分别为75μm和100μm,由盖片对其进行密封。考察了有绝缘漆模具和无绝缘漆模具制作的芯片的电泳分离情况。在该PDMS微流控芯片上对用异硫氰酸酯荧光素标记的氨基酸进行了电泳分离,当信噪比S/N=3时,最小检测浓度达到0.8×10-11mol/L。 相似文献
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Yaqiong Li Lili Li Zhengxiao Liu Mingyu Ding Guoan Luo Qionglin Liang 《Microfluidics and nanofluidics》2016,20(7):97
Oxygen participates in numbers of cellular activities and behaviors in both normal and pathological tissues. In physiological microenvironment, oxygen tension is generally below 21 % and varies in different species, states and regions of organs. However, present studies of cellular behavior in vitro are performed in an ambient level, which is not conformity to the reality in vivo. In this study, a microfluidic device was developed to generate controllable oxygen tensions on a multiple-channel array chip for high-throughput drug screening. Controlling various concentrations of chemical reagents with confined flow rate, specific oxygen tensions can be established from 1.6 to 21 %, where the oxygen tension of each channel can be modulated in demand. When the concentrations of pyrogallol change from 100 to 700 μg/mL with the flow rate of 5 μL/min, oxygen tensions in cell chambers range from 12.5 to 3.87 %. Pyrogallol with the concentration of 0 μg/mL is used as the control group to obtain 20.9 % oxygen condition. The developed microfluidic chip was used to investigate the cytotoxicity of TPZ and cisplatin, and the results demonstrate different manners of two oxygen-sensitive anti-tumor drugs in oxygen-dependent cytotoxic responses. Due to its character, the microfluidic device is believed to establish any desired and measurable oxygen tension distribution for pharmacology development, which is promising to improve efficiency and reduce tedious operation for pharmaceutical studies. 相似文献
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This article describes a superposable double-concentration-gradient droplet array chip, which allows a variety of concentration combinations of two components to be formed simultaneously. The concentration gradients generated from two layers of the chip could be arbitrarily superimposed by adjusting the center angle between the two bonding layers. With the aqueous phase flow rate of 1.0 μL min?1 and the oil phase flow rate of 30.0 μL min?1, the droplets about 58 μm in diameter were produced, and the coefficients of variation were below 6.0% for single channel and 5.7% for all the channels. Using a dual-32-channel superposable gradient droplet array chip, poly(ethylene glycol) diacrylate (PEGDA) microspheres containing concentration-gradient combinations of rhodamine B and fluorescein were fabricated to demonstrate the capability of PEGDA for encapsulating hydrophilic and hydrophobic substances, as well as the proper concentration-gradient distribution. Furthermore, PEGDA microspheres loaded with two anticancer drugs, hydrophilic doxorubicin hydrochloride and hydrophobic paclitaxel, of 17 concentration combinations were simultaneously prepared. The drug-induced apoptosis of human uterine cervix cancer cells was investigated using the dual-drug-loaded PEGDA microspheres. The optimum synergistic concentration combination of the two drugs was 12.5 μg mL?1 for doxorubicin hydrochloride and 43.75 μg mL?1 for paclitaxel according to the preliminary screening. The superposable double-gradient droplet array generator was demonstrated to be a promising platform for screening multiple drug combination in microcarriers. 相似文献
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新型的细胞外电信号传感芯片是采用0.6 μm标准CMOS工艺设计制造,片上集成了6×6单元有源传感阵列、模拟多路选择器、输出缓冲器、参考源和数字控制电路.有源传感单元面积为60μm×60μm,包含15μm×15μm的传感电极和预处理电路,能够线性放大幅值范围100μV~25 mV的微小信号,电压增益为40dB.并采用相关二次采样工作模式降低固定模式噪声,提高传感器的精度.在标准CMOS工艺基础上,应用无电浸镀金改进传感电极的生物兼容性,并采用特殊封装技术提高芯片在溶液环境中的稳定性.溶液中模拟生物信号测量验证了该芯片的功能. 相似文献
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Peng Xue Kai Ye Jie Gao Yafeng Wu Jinhong Guo Kam M. Hui Yuejun Kang 《Microfluidics and nanofluidics》2014,16(3):605-612
Circulating tumor cells (CTCs), which are derived from primary tumor and circulate to secondary site, are regarded as the cause of metastasis. Many methods have been applied for CTC isolation and enumeration so far. However, it remains a challenge to effectively elute the captured cells from the device for further cellular and biomolecular analyses. In this paper, we fabricate a dual-functional herringbone chip to achieve both CTC capture and elution based on the immunoassay of epithelial cell adhesion molecule antigen expressed on the surface of human liver cancer cell line Hep3B. The results show that the capture limit of Hep3B cells can reach as low as 3 cells per ml with capture efficiency over 50 % on average. On the other hand, the elution rate of more than 50 % of the captured Hep3B cells can be achieved for cell density ranging from 5 to 2 × 103/ml. It demonstrates that this herringbone chip exhibits excellent dual functions with high capture efficiency and considerable elution rate, indicating its promising capability for clinical assay in cancer diagnosis. 相似文献
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一个超高速FFT阵列式计算结构设计方案 总被引:1,自引:0,他引:1
本文分析了CORDIC算法和FFT算法的在内在联系,设计了基于CORDIC算法的四个蝶形运算器芯片,并在此基础上构成了FFT阵列式计算结构,N=2^m点的FFT计算速度可达到微秒级,有很好的性能人格比,在超高速实时信号处理中有广阔的应用前景。 相似文献
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Jingdong Chen Di Chen Tao Yuan Xiang Chen Jun Zhu Andreas Morschhauser Joerg Nestler Thomas Otto Thomas Gessner 《Microsystem Technologies》2014,20(3):485-491
Microfluidic chips were designed and fabricated to capture cells in a relative small volume to generate the desired concentration needed for analysis. The microfluidic chips comprise three-dimensional (3-D) cell capture structures array fabricated in PDMS. The capture structure includes two layers. The first layer consists of spacers to create small gap between the upper layer and glass. The second layer is a sharp corner U-shaped compartment with sharp corners at the fore-end. And another type capture structure with Y-shaped fluidic guide has been designed. It was demonstrated that the structures can capture cells in theory, using Darcy–Weisbach equation and COMSOL Multiphysics. Then yeast cell was chosen to test the performance of the chips. The chip without fluid guides captured ~1.44 × 105 cells and the capture efficiency was up to 71 %. And the chip with fluid guides captured ~5.0 × 104 cells and the capture efficiency was ~25 %. The chip without fluid guides can capture more cells because the yeast cells in the chip without fluid guides are subject to larger hydrodynamic drag force. 相似文献
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Vertical Si nanowires with ultra-high-aspect-ratio were fabricated using a combined process of deep reactive ion etching and sacrificial oxidation. The combined process starts with etching the Si substrates by the Bosch process to form micrometer-scale structures. The etched micrometer-scale structures are shrunk to nanometer-scale by sacrificial oxidation. The fabricated Si nanowires that were aligned vertically to the substrate had a diameter of less than 200 nm and a length greater than 10 μm. One of the fabricated Si nanowires had a diameter of 110 nm and a length of 11 μm. The resulting aspect-ratio reached 100, which is a value that is significantly high for vertical Si nanowires fabricated by using a top-down approach. 相似文献
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A basic event in amoeboid-type cell movement (ATCM) is extension of an exploratory cell protrusion called a pseudopod. ATCM
of cells among the fibrous macromolecules of the extracellular matrix (ECM) is crucial to tissue development and wound healing,
and a key process in immune response and cancer invasion. We studied ATCM by developing a computational model of pseudopod
extension/retraction driving ECM sensing, cell-matrix adhesion, and cell translocation within the 3D ECM mesh. We were successful
in producing a biomorphic ATCM motion cycle involving viscoelastic protrusion and attachment to ECM, cell body displacement,
and cell detachment from old adhesion sites. The simulated trajectories were in general persistent random walks resembling
ATCM patterns reported for patrolling immune cells and cancer cell invasion. 相似文献