Lon网到Ethernet网关的研究实现

通过对LonWorks现场总线技术和以太网技术的分析，阐明了两网互联的意义和实现方法；设计开发了实现两网互联的网关设备——互联适配器，介绍了LonWorks的Neuron芯片通信模块的硬件电路设计、LonWorks通信模块与以太网通信模块中51单片机的接口硬件电路设计部分和以太网接口通信模块的硬件电路设计，以51单片机为主CPU和以Neuron芯片为从CPU的软件设计。     

BGT脑功能测试系统的研制

ＢＧＴ脑功能测试系统是在格式塔测验简便、易行的基础上进行创新的。改变了原始手工操作方式，使该测试技术智能化和高效率。引用计算机处理技术，研制出专用的硬件和软件并制作成专用的诊断设备，提出了该测试技术信度和效度，从而提高了心理测验的水平和档次，填补了我国在脑功能心理测验专用测试系统硬件的软件方面的空白。     

便携式多点温度采集系统

系统采用DS18820-PAR单总线工作方式和无需内部单独供电的寄生电源供电工作方式，解决了传统的测温系统硬件结构复杂、测温系统中的引线误差补偿问题。软件设计中采用多传感器Kalman滤波技术、软件容错技术，实现多信号的采集和数据处理。在长距离测量中，给出了系统硬件匹配中的改进意见。通过货仓应用，验证了系统硬件构成的可行性和优点。     

基于COM技术和OPC规范的开放式结构数控系统研究

对COM技术和OPC规范进行简要介绍后，指出基于COM技术/OPC规范的开放式数控系统的优势所在。提出了开放式结构数控系统的层次模型和工作流程。并以此为基础。研究了一个实例系统的软硬个结构。该实例的硬件采用自行开发的DSP运动控制卡，软件采用COM技术，硬件驱动程序符合OPC规范。     

基于LabVIEW的桥梁运行状态长期监测系统的设计

论述铁路桥梁运行状态长期监测系统的设计方法，介绍它的硬件组成、软件结构以及系统功能，本系统利用网络传感器、以太网技术和虚拟仪器技术，在LabVIEW平台上实现了现场数据的网络化远程传输、监控和数据查询。     

单片机数据采集系统与TMS32OC31通信技术的研究

本文就硬件构成原理和软件设计方法，介绍了DSP芯片与基于传统单片机的数据采集系统通信的技术。实践表明，由此方法设计的硬件简洁明了，且系统具有很好的灵活性和可扩展性，此方法有很强的实用价值。     

基于FPGA电路重构技术的电子系统设计

文中通过应用实例详细地介绍FPGA电路重构技术。在充分了解FPGA的PS配置模式时序的基础上，用单片机AT89C51模拟实现PS配置时序，从而实现FPGA电路重构技术。文中给出了详细的系统硬件电路图和单片机程序流程图。为了说明电路重构技术，通过开关可以选择系统的两种不同的系统结构，分别是数字显示电子钟和18路电子抢答器，因而实现了系统硬件电路的重构。     

逆向工程--技术创新的现代化手段

逆向工程技术是技术创新的现代化手段，它能很好地把继承和创新相结合。介绍逆向工程技术所使用的硬件、软件、开发方法、应用举例、模具开发。     

计算机控制光电跟踪仿形方法的研究

探讨计算机控制光电跟踪仿形的方法。与通常用硬件实现的光电跟踪仿形不同，这种方法是以现代图形图像处理技术为基础，结合光电转换技术，采用计算机软件的方法实现的，硬件系统结构简单，适应范围广，具有良好的应用前景。     

一种新型温控仪的硬件设计

采用了当前先进的嵌入式芯片技术，针对传统温控仪的设计方法，介绍了一种基于PSoC微控制器的温控仪的硬件设计。由于PSoc内部具有多个模拟模块和数字模块，因此可以被合理地配置成用户需求的模拟电路和数字电路，从而使该硬件设计具有结构简单、功能多、成本低的特点。     

Histochemical and ultrahistochemical localization of heavy metals in calf organs

Cadmium, zinc, selenium, and copper were administered, singly or in combination, orally or subcutaneously. Experiment I included 32 calves of both sexes; six received Cd (two groups), Zn, Cd, and Zn, and Cd and Se (two groups) and one group was a control. In Experiment II (21 bulls), three were given Cd, Cd, and Cu, and Cd and Zn, respectively, and one group was a control. For light microscopy, in Experiment I the highest amounts of silver granules were present in the samples of liver, small intestine, and vesicular gland of all the exposed groups; in Experiment II the most affected organs were liver, kidney, and small intestine. For electron microscopy, in Experiment I, after administration of Cd and Zn, the highest amounts of granules were seen in the cytoplasm of hepatocytes and cells of the proximal and distal renal tubules and the lowest amounts were found in glandular cells of the pancreas. Administration of Cd and Se resulted in the presence of large numbers of granules in the nuclei and nucleoli of spermatogonies. In Experiment II, ingestion of Cd and Zn in feed led to the appearance of highest amounts of granules in the nucleoli, nuclei, and cytoplasm of cells in testes, kidneys, and pancreas. Following Cd intake, the highest accumulation of granules was observed in the nucleoli of hepatocytes and cells of the proximal and distal renal tubules. Combined Cd and Cu produced the highest number of granules in cells of the proximal and distal renal tubules and in the nucleoli and nuclei of germinal epithelium.     

智能机床发展现状与技术体系框架

智能机床成为机床装备发展的趋势。在介绍国内外智能机床的研究现状的基础上,分析目前智能机床技术研究的三个问题,提出智能机床的狭义和广义定义;给出智能机床的包括人计机的协同性、整体与局部的协调性、智能的恰当性与无止性、自学习及其能力持续提高性、自治与集中的统一性、结构的开放性和可扩展性、制造和加工的绿色性、智能的贯穿性八个智能技术特征;阐述包括以人为中心的人计机动态交互功能、三个基本功能——执行智能功能、准备智能功能、维护智能功能的智能功能特征,以及功能之间的相互关系;建立八个理论与技术的智能机床技术理论框架,包括人计机一体化融合理论与技术、多源信息的感知理论与技术、智能决策理论与技术、智能执行理论与技术、智能维护理论与技术、智能机床综合能力评价理论与技术、智能机床标准化技术、智能机床支撑技术等,形成了智能机床的技术体系框架,同时指出上述内容将随着技术的进步和应用的深入不断充实和丰富。     

Effects of polydatin on the proliferation,migration, and invasion of ovarian cancer

To investigate the effects of polydatin on the proliferation, migration, and invasion of ovarian cancer, the change of proliferative ability, migration ability, and invasive ability of human ovarian cancer cell OVCAR-3, A2780, and HO-8910 was detected by using polydatin and up-regulating PI3K. The anticancer activity and mechanism of polydatin in ovarian cancer were analyzed. Polydatin could effectively inhibit the proliferation, migration, and invasion of OVCAR-3, A2780, and HO-8910, and inhibit the expression of PI3K protein. After the expression level of PI3K protein was up-regulated, the inhibitory effect of polydatin on the proliferative ability, migration ability, and invasive ability of OVCAR-3, A2780, and HO-8910 significantly decreased, suggesting that PI3K was the target of polydatin. Therefore, we concluded that polydatin could inhibit the proliferation, migration, and invasion of ovarian cancer cells by inhibiting the expression of PI3K protein, which provides an experimental basis for polydatin in the treatment of ovarian cancer.     

Semiautomated methods for cancellous bone histomorphometry using a general-purpose video image analysis system

Semiautomated methods are used to measure elongated, curved and complex branching profiles and isolated perimeter segments in monochrome video images with a general-purpose analysis system. These methods are used to make the major primary measurements of bone histomorphometry. Accuracy and reproducibility of the image acquisition, processing and measurement system is documented by measuring a semicircular standard of known dimensions. Semiautomated applications of the Ar/Le method for measuring areas and perimeters, and calculating lengths and widths of osteoid seams, lengths of mineralization labels and mineral apposition rate, wall width, indirect measurements of eroded, osteoclastic and osteoblastic perimeters without tracing, and measurement of mineralized or total cancellous bone area and perimeter gave values comparable to measurements of the same parameters by tracing or grid counting techniques with equal or better reproducibility and much greater efficiency. Intraindividual variation in measuring multiple bone biopsies was comparable to that reported with current standard methods. Major sources of variability for semiautomated methods were image magnification and selection of profile edges by thresholding, and sources of variability for manual methods are image magnification, numbers of orthogonal intercepts, tracing speed and accuracy of the algorithm used to measure traced pixels. Semiautomated methods are accurate, reproducible and rapid methods suitable for bone histomorphometry.     

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

In recent years, microbial metabolomics, a new field that has attracted wide attention, provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts. Many microorganisms are found in the human intestine, oral cavity, vagina, etc. Probiotics could maintain the good health of the host, while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind. Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system, which could provide key information to understand the related metabolic pathways and associated changes. This approach analyzes the final products of cellular regulatory processes, the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes. Microbial metabolomics has been widely used in different research fields, such as microbial phenotypic classification, mutant screening, metabolic pathways, microbial metabolic engineering, fermentation engineering monitoring and optimization, microbial environmental pollution, and so on. However, there are only a few reviews on microbial metabolomics of probiotics and pathogens. This review summarizes the main methodologies, including sample preparation, identification of metabolites, data processing, and analysis. Recent applications in microbial metabolomics of probiotics and pathogens are also described. This paper first summarized the research progress and application of microbial metabolomics from two aspects: probiotics and pathogenic bacteria. Probiotics and pathogenic bacteria do not exist independently most of the time; hence, these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria, which was subdivided into important microbial research fields closely related to human health, including the human gut, oral cavity, food, and nutrition-related microorganisms. Then, the main problems and trends associated with microbial metabolomics are discussed.     

生物轻质高强结构及其在吸能结构中的仿生应用

在长期进化过程中，自然界中的多种动物、植物形成了独特的轻质、高强结构，以此来抵抗外界的复杂冲击载荷，保护自身完整，满足生存需要。生物轻质高强结构的优越性，启发了科研和工程人员采用结构仿生学的方法来对管状和板状两大类吸能结构进行设计优化和改进。对竹子、茎秆/树干、羽轴、骨骼四类管状生物结构和甲虫鞘翅、贝壳、柚子皮、龟壳四类板状生物结构进行综述，阐述了分层、多孔、螺旋、中空等多种结构与轻质高强特性之间的关系。在此基础上，对比和分析了相应的结构元素在单胞管、多胞管、嵌套管、波纹管等管状吸能结构和蜂窝夹芯板、复合材料板、混合结构板等板状吸能机构中起到的作用。进一步对当前仿生吸能领域存在的结构复杂、质量大、缺乏普适性的机理和过渡“桥梁”等问题做出了分析；最后对仿生吸能技术的形式简单化、结构轻量化、理论通用化、“形神兼备”化发展趋势做出展望。     

基于OCS的压力计量授权系统

介绍了应用OCS控制器实现压力仪表的计量采集和系统授权管理,压力仪表具有4 mA~20mA、RS485、HART及RJ45等多种电气接口,工业一体化PLC控制器OCS自身具备多种IO端口,可以直接应用OCS采集压力仪表的实时数据和配置参数。同时,设计出一套授权模型以满足设备用户进行权限授权和商务追款的技术需求,依次通过授权码合法性判断、操作时效性限制、授权时效性约束等实现压力计量授权系统的授权管理。经测试验证,所设计的压力计量仪表授权系统可以较稳定高效地实现压力仪表数据采集和设备授权管理,达到了预期的设计效果。     

Localization of sarcoglycan, neuronal nitric oxide synthase, beta-dystroglycan, and dystrophin molecules in normal skeletal myofiber: triple immunogold labeling electron microscopy.

In order to investigate the mode of existence of the sarcoglycan complex, neuronal nitric oxide synthase (nNOS), beta-dystroglycan, and dystrophin in the normal skeletal myofiber, we examined the ultrastructural localization and mutual spatial relationship of nNOS, beta-dystroglycan, dystrophin, and the individual components of the sarcoglycan complex by using triple immunogold labeling electron microscopy. Each molecule of alpha-, beta-, gamma- and delta-sarcoglycans is located intracellularly or extracellularly near the muscle plasma membrane mostly in accordance with the sarcoglycan antigenic sites against which the antibodies were generated. The association of different two and/or three sarcoglycan molecules out of alpha-, beta-, gamma- and delta-sarcoglycan molecules was frequently observed. Each molecule of nNOS, beta-dystroglycan, and dystrophin was ultrastructurally noted along the cell surface of normal skeletal myofibers. Moreover, the close relation of a sarcoglycan molecule with beta-dystroglycan and dystrophin, and the association of nNOS with dystrophin were also confirmed ultrastructurally. Thus, this study demonstrated that the constituting molecules of the sarcoglycan complex, nNOS, beta-dystroglycan, and dystrophin existed in the form of a cluster at the normal muscle plasma membrane. The association of nNOS with dystrophin and its associated glycoproteins may form a macromolecular signaling complex at the muscle plasma membrane.     

Micromorphology,pharmacognosy, and bio‐elemental analysis of an important medicinal herb: Verbascum thapsus L.

The present study was conducted on characterization of morpho‐anatomical, phytochemical, and bio‐elemental analysis of root, stem, and leaf of Verbascum thapsus. Morphologically Verbascum is a biennial plant that flowers for a month and a half in mid‐ to late summer. Various organoleptic features of root, leaf, and stem were recorded. Anatomically the T. S of the root, stem, and leaf showed a typical dicot histological differentiation. Leaf possessed anomocytic stomata, crescent shape vascular bundles, and covered with long and stellate type trichomes while, stem contained collateral type of vascular bundles and a well‐developed pith to store phytochemicals responsible for various pharmacological activities. The powder drug study through scanning electron microscopy revealed the presence of various types of tissues. Branched, tree like and stellate trichomes in root and leaf help in absorption and reduce loss of water. These anatomical features are responsible for the survival of the plant as biennial. Four macro elements (Na, K, Ca, and Mg) and seven microelements (Cr, Cu, Fe, Mn, Ni, Zn, and Cd) and their concentrations in ppm were also studied using Atomic Absorption Spectroscopy. Phytochemical screening of methanolic extract showed existence of various secondary metabolites, while mucilage and anthraquinones was not detected. The present study helps to understand the taxonomic identification of the plant based on morpho‐anatomical features and throws the attention of the researchers to carry out the work for developing its various formulations, which can ultimately be beneficial for the human beings as well as animals.     

A simplified wet friction clutch test apparatus

A small, economic, test rig to evaluate friction materials and fluids for wet clutch applications has been designed, built and commissioned. In this paper, the construction, operation and performance of the machine are described, and results are presented, for two sets of typical tests. The system is capable of recording data, and torque test track, coefficients of friction, and oil temperature rise, are given.