Sting challenge outcome as a selection criterion for venom immunotherapy

We report a case of dysplastic arterial vascular abnormality in a 32-year-old man with overlying neuronal cell migration disorder. MR images showed a thickened left insular cortex adjacent to the abnormal vascular network. These findings suggest the possibility of leptomeningeal damage during neuronal cell migration as the cause of the overlying vasculopathy. The true pathogenesis of these seemingly associated abnormalities is unknown.     

Soft tissue neck radiography for displaced dental prostheses

Modern dental prostheses continue to be made of acrylic resins which can be difficult or impossible to recognize by conventional radiography. We discuss the difficulties that can arise and the recognition of such objects in the neck using three illustrative cases.     

Absorption of the anxiolytic pazinaclone in animals as a criterion for species selection for toxicity studies

In rats, mice, hamsters, and guinea pigs given a 5 mg/kg oral dose of pazinaclone (CAS 103255-66-9), unchanged drug concentration in plasma was highest in mice (AUC; 90 ng.h/ml), followed in decreasing order by guinea pigs (AUC; 41 ng.h/ml), hamsters (AUC; 18 ng.h/ml), and rats (AUC; 17 ng.h/ml). In terms of plasma drug concentrations and toxicological background data, there was no better alternative rodents than mice and rats for the toxicity studies. Among rabbits, dogs, and monkeys, the dogs had the highest plasma drug concentrations: AUCs of pazinaclone in dogs and monkeys were 1035 and 458 ng. h/ml, respectively (drug concentration in rabbit plasma was very low). Of the two polymorphs, forms 1 and 2 with particle size of < or = 5 microns, the oral absorption of form 2 in rats was more efficient than that of form 1 at 1000 mg/kg: AUCs of pazinaclone after dosing of form 1 and 2 were 489 and 965 ng.h/ml, respectively. However, form 1 was selected for the toxicity studies because of the poor physico-chemical properties of forms 2. Form 3 was not included in this study, because this form was relatively unstable and contained relatively large amount of impurities. The absorption of pazinaclone in dogs was improved by decreasing its particle size: AUCs of pazinaclone after dosing of the drug having particle size of 5.5, 20.8, and 79.3 microns were 1361, 822, and 297 ng.h/ml, respectively. Since large-scale preparation of bulk pazinaclone with a particle size of 5 microns or smaller was not feasible, the drug having a particle size of about 20 microns was used in the toxicity studies. The absorption of pazinaclone was more extensive when the drug was given to fed animals as suspension. Thus, the toxicity studies were performed using form I of pazinaclone with a particle size of about 20 microns primarily in rats, mice, and dogs.     

Correction of background noise in direct digital dental radiography

OBJECTIVES: To clarify the source of noise in direct digital intra-oral radiography with RVG-S (Trophy Radiologie, Vincennes, France) and to use these to develop a method for correction of background noise. METHODS: Sensor temperature, image acquisition time and X-ray dose were independently analysed with the IPLab Spectrum (Signal Analytics, Vienna, VA) software. RESULTS: The decrease in pixel value due to the dark current was linearly related to the image acquisition time. Although a variation in sensitivity was observed when the sensor was exposed to X-rays, the mean pixel value of the entire image was linearly related to the exposure time. The image showing only the signal due to X-ray dose was derived from the original RVG-S image by correcting for the dark current and the pixel-by-pixel sensitivity variation of the CCD sensor. CONCLUSION: The image formed only by X-ray dose distribution can be derived by correcting for the background noise.     

Validation of an in-office dental unit water monitoring technique

OBJECTIVE: Intestinal transplantation has become an option as a treatment for permanent intestinal failure. Endoscopy is an essential tool in assessing the intestinal allograft after intestinal transplantation. The aim of this study was to analyze our experience using endoscopy in intestinal transplant recipients. METHODS: This was a retrospective review of endoscopic and histological reports in 41 children who received an intestinal transplant between 1990 and 1995 at Children's Hospital of Pittsburgh. RESULTS: A total of 1273 endoscopies was performed of which 760 were ileoscopies via allograft ileostomy, 273 were upper endoscopies, and 240 were colonoscopies. One hundred four rejection episodes were documented histologically in 32 patients, 6 days to >4 yr after transplantation. Most episodes were mild and easily treated with increased immunosuppression; however, severe rejection with mucosal exfoliation was seen in nine patients. Rejection sometimes involved only part of the allograft. Endoscopic appearance alone without biopsies was sensitive enough to diagnose only 63% of the rejection episodes. Epstein-Barr and cytomegalovirus infections occurred in 11 and eight patients, respectively, and involved both native bowel and allograft in some. Complications of endoscopy were few: one perforation, three episodes of bleeding, and three episodes of transient respiratory compromise. CONCLUSIONS: Endoscopy is an essential tool in the postoperative assessment of intestinal transplant recipients. Frequent surveillance ileoscopies with biopsies should be performed after transplantation. If patients clinically deteriorate with fever, diarrhea, bacteremia, or gastrointestinal bleeding and a clear cause is not elucidated by ileoscopy, an upper endoscopy with biopsies is indicated.     

Validation of the Beck Depression Inventory in a university population using psychiatric estimate as the criterion.

Investigated the utility of the Beck Depression Inventory for survey use in a college population by determining its concurrent validity, using psychiatric rating of depth of depression as the criterion. 56 undergraduate and graduate students served as Ss. Interrater reliability of psychiatric estimate as measured by a Pearson product-moment correlation coefficient was .62, perhaps because the primary and secondary raters used different diagnostic procedures. The Pearson-product-moment correlation coefficient between the inventory and the psychiatric rating was .77. These findings indicate that the Beck Depression Inventory is a valid instrument for use in a college population. The Pearson product-moment correlation coefficient between the inventory and the psychiatric estimate fell to .30 in a 2nd sample of 27 Ss with whom 1–24 days intervened between administration of the inventory and the psychiatric interview. This attenuation in Ss who experienced a time delay is consistent with the nature of the depression inventory as a measure of state as opposed to trait depression. The apparent decline in measured depression additionally suggests the need for longitudinal study to determine its course and outcome. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Analysis of immunoglobulin-synthesizing cells in human dental periapical lesions by in situ hybridization and immunohistochemistry

The iron status of 22 children and adolescents with Crohn's disease (mean age: 13 years) was evaluated. Eleven patients were suffering from active disease with inflammation, identified by at least one abnormal value for serum orosomucoid, C-reactive protein or sedimentation rate (group I). Eleven patients were in clinical remission and showed no biological evidence of inflammation (group II). Hemoglobin and red cell indices, erythrocyte protoporphyrin, serum iron, transferrin, serum ferritin and basic red cell ferritin were determined in all patients. The usual indicators of iron status, particularly serum ferritin, were affected by the inflammatory processes, but basic red cell ferritin appeared to be independent of inflammation. Basic red cell ferritin can therefore be considered to be a reliable indicator of iron status in children and adolescents with Crohn's disease.     

Validation of a telephone questionnaire for Parkinson''s disease

Unfractionated as well as low-molecular-weight heparins (LMWH) are known to cause an increase in blood levels of tissue factor pathway inhibitor (TFPI). To study the effect of a newly developed supersulfated LMWH (IK-SSH, Iketon Farmaceutici) on TFPI concentrations in human plasma, the compound was injected into volunteers at doses of 0.14, 0.33 and 0.66 mg/kg intravenously or 0.33, 0.66 and 1.0 mg/kg subcutaneously. At certain known times blood was drawn and plasma levels of both total and free TFPI were measured using enzyme-linked immunosorbent assay methodology. Baseline plasma concentrations of TFPI were 72.2+/-3.1 ng/ml for total and 10.8+/-0.8 ng/ml for free TFPI. Intravenous or subcutaneous injection of IK-SSH led to a strong and long-lasting rise in TFPI levels which were increased more than 5-fold for total TFPI and more than 30-fold for free TFPI. Maximum TFPI levels were reached 5-10 min after intravenous and 60 min after subcutaneous administration. IK-SSH caused prolongation of ex-vivo clotting times in the APTT and Heptest assay, whereas thrombin time was not affected. Anticoagulant actions of IK-SSH showed a significant correlation to plasma concentrations of TFPI and they are thought to be based at least partially on the release of TFPI from vascular sites.     

Is amenorrhea a critical criterion for anorexia nervosa?

The significance of amenorrhea as a criterion for anorexia nervosa was examined. Twelve nonamenorrheic women treated for anorexia were compared with 40 women meeting full DSM-IV criteria. The nonamenorrheic group displayed the same high levels of eating disorder, body-image disturbance, and psychopathology as the amenorrheic group, as measured by the following variables: body-size overestimation on the Image Marking Procedure; body distortion on the Body Distortion Questionnaire; eating disorder on the Eating Disorder Inventory; depression on the Beck Depression Inventory; psychopathology on the MMPI; and external locus of control on the Rotter Locus of Control Scale. Amenorrhea does not appear to be a useful criterion for distinguishing full-syndrome anorexia nervosa from partial-syndrome cases.     

Use of exit examinations: a criterion for graduation?

This study sought to measure the use of exit examinations in nursing schools at Historically Black Colleges and Universities (HBCU). Fifteen participants from HBCU nursing schools throughout the United States were surveyed to determine current practices related to exit exams. Overall, fourteen schools (93.33%) used an exit exam at the end of their nursing program. However, 73.33% of the participants (11 schools) reported it was mandatory for students to pass the exam as a requirement for graduation. Almost 47% of the participants (7 schools) reported the use of the exit exam was related to NCLEX-RN pass rates, while others reported identification of student needs as the primary reason for using an exit exam. Most participants were very helpful by sharing their innovations. While this small study provided some information on the use of exit examinations, more research is needed to substantiate both the appropriateness and usefulness of their use in baccalaureate degree nursing programs.     

Relative distribution of plasma cells expressing immunoglobulin G subclass mRNA in human dental periapical lesions using in situ hybridization

Mutation of the BRCA1 gene in well-defined breast cancer families has been associated with an 87% lifetime risk for breast cancer and a 44% risk for ovarian cancer. Recent data indicate that the risk associated with these mutations is considerably lower, although still far greater than the risk for disease in the rest of the population. Approximately 81% of the mutations that have been identified have been frameshift (71%) or nonsense (10%) mutations, and either may result in a truncated protein. The protein truncation test (PTT) is often used to screen patients at high risk, because sequencing of this large (100 kb) gene with its 22 coding exons is an arduous task. The PTT was used to analyze genomic DNA and RNA from the peripheral blood of a 31-year-old Filipino woman with a poorly differentiated, stage 2A breast carcinoma and a family history of breast-ovarian cancer. PTT identified the wild-type protein fragment and an additional truncated protein fragment in the patient's sample. Subsequent direct sequencing of the appropriate coding region revealed a point mutation in exon 11 at nucleotide 2178, resulting in a C > T transition that caused a termination (stop codon) in amino acid 687. To our knowledge, this is the first report of mutation of the BRCA1 gene in a Filipino family, and this in-frame stop-codon mutation has not been reported previously.     

Patient collaboration as a criterion for the therapeutic alliance.

In this article we show that a focus on patient collaboration, as a marker for the therapeutic alliance, is advantageous not only for theory and research but also for clinical practice. This point of view in no way minimizes the importance of the therapist's activity or the clinical significance of the interaction between patient and therapist. Rather, it focuses attention on how the therapist's personality, skill, and technique interact with the problems the patient brings to psychotherapy. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Validation of a multiple-assessment procedure for managerial personnel.

Amultiple-assessment procedure—personal history form, intensive interview, 2 objective intellectual aptitude tests, a sentence-completion test, and a human relations problems test—was used to predict the performance of 31 industrial managers all having a similar job assignment. Predictions were made on the basis of a global, nonactuarial analysis of these objective and subjective data. 4 sets of criterion judgments were obtained on 4 variables—3 different sets of rankings and 1 set of ratings. A multitrait-multimethod matrix was used in the analysis of the intercorrelations. 9 of the 12 validity coefficients involving ranking-type criteria were statistically significant. Of the 4 coefficients involving rating-type criteria, none was significant. (18 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)