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金黄色葡萄球菌(Staphylococcus aureus)作为一种食源性致病菌,是导致食品安全和公共卫生事件的主要病原体之一,开发快速准确的金黄色葡萄球菌检测技术是保障食品安全和提高食源性致病菌防控水平的关键措施。核酸等温扩增技术具有检测速度快、灵敏度高和设备简单等优点,在金黄色葡萄球菌检测方面具有很大的应用前景。基于此,该文简要介绍了核酸等温扩增技术的原理及优缺点,主要阐述了核酸等温扩增技术在金黄色葡萄球菌检测上的应用,并总结了目标产物检测技术,为金黄色葡萄球菌等温扩增快速检测技术的开发提供理论支持,为食品安全控制和食源性致病菌监控工作奠定基础。 相似文献
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大肠杆菌O157:H7、单核细胞增生李斯特菌、金黄色葡萄球菌和沙门氏菌是引起食物中毒的主要致病菌。目前的常规检测技术均不同程度存在耗时长、费用高、易污染等问题,成为了政府有效开展食源性致病菌食物中毒事件应急方案的瓶颈。快速、灵敏、方便、实时的新型致病菌检测技术的研究成为国内外的科研热点。本文综述大肠杆菌O157:H7、单核细胞增生李斯特菌、金黄色葡萄球菌和沙门氏菌的污染现状,总结新型PCR技术、表面等离子共振技术、生物传感器技术、免疫识别为基础的检测技术、免疫磁分离为基础的检测技术、毛细管电泳技术7大新型致病菌检测技术的研究现状。 相似文献
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PCR技术在食源性致病微生物快速检测中的应用 总被引:2,自引:0,他引:2
PCR(Polymerase Chain Reaction)即聚合酶链式反应,是一种体外快速扩增基因或DNA序列的方法。食源性致病微生物是影响食品质量和安全的主要因素之一,建立和完善食品中致病微生物快速检测技术具有重要的现实意义。PCR技术以其自身的灵敏度高、特异性强、速度快等优点作为食源性致病微生物检测的关键技术在食品中得到广泛的应用。本文简要介绍PCR技术的基本原理,及其在几种食源性致病微生物如沙门氏菌、金黄色葡萄球菌、单核细胞增生李斯特氏杆菌、致病性大肠杆菌及其他一些有害微生物检测中的应用,并分析了PCR技术在食源性致病菌检测的实际应用中存在的一些问题及对此项技术的展望,这将有力促进我们今后更好地研究应用PCR技术检测食品中食源性致病微生物。 相似文献
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实时荧光定量PCR技术作为一种高效的微生物定量检测法,在食品工业中具有广泛的应用前景。本文综述了实时荧光定量PCR技术检测原理,及其在食源性致病菌如金黄色葡萄球菌、大肠杆菌O157∶H7、沙门氏菌和副溶血性弧菌检测中的应用。 相似文献
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分别针对副溶血弧菌及金黄色葡萄球菌的特异性基因blaCARB-17、nuc设计引物,通过优化反应条件,建立一种能够同时检测副溶血弧菌和金黄色葡萄球菌的实时荧光环介导等温扩增(real-time loop-mediated isother-mal amplification, RT-LAMP)的检测方法,对其特异性、灵敏度和适用性进行了研究。结果表明该方法特异性好、灵敏度高,对副溶血弧菌和金黄色葡萄球菌的检出限分别为3.62×102、1.45×104 copies/mL,灵敏度是普通PCR方法的10倍。实现了一次LAMP反应中同时检测2种食源性致病菌,既缩短检测时间,也提高了检测效率,为多重LAMP检测食源性致病菌的研究提供参考。 相似文献
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Sonal Patil Paula Bourke Bridget Kelly Jesus M. Frías P.J. Cullen 《Innovative Food Science and Emerging Technologies》2009,10(4):486-490
Inactivation of Escherichia coli in liquids was carried out using power ultrasound. Parameters examined included amplitude levels (0.4 µm, 7.5 µm, 37.5 µm), treatment time, cell condition (non-adapted cells, acid adapted cells), liquid media (TSB, model orange juice and model apple juice) and E. coli strain (ATCC 25922, NCTC 12900). The efficacy of ultrasound treatment was found to be a function of amplitude level, treatment time and media (p < 0.05). The kinetics of inactivation followed zero order kinetics (R > 0.95), with the highest inactivation achieved using an amplitude of 37.5 µm. The D-values of E. coli 25922 at all amplitudes in model orange juice were not significantly different than in TSB media. However, at 0.4 µm and 37.5 µm amplitude D-values of E. coli 12900 were significantly different in model orange juice compared to TSB media. When efficacy of ultrasound was assessed in model apple juice and phosphate buffered saline treatment times were significantly reduced by comparison with TSB. Inactivation of E. coli was found to be influenced by strain, prior acid adaptation and suspension liquid, but the effect was negated at the higher amplitude levels.
Industrial relevance
To facilitate the preservation of unstable nutrients many juice processors have investigated alternatives to thermal pasteurisation, including un-pasteurised short shelf life juices with high retail value. This trend has continued within the European Union. However within the US recent regulations by the FDA have required processors to achieve a 5-log reduction in the numbers of the most resistant pathogens in their finished products. This rule comes after a rise in the number of food borne illness outbreaks and consumer illnesses associated with consumption of untreated juice products. Pathogenic E. coli may survive in acid environments such as fruit juices for long periods. Ultrasound has been identified as one possible non-thermal technology to meet the required microbial log reduction. However it is important to determine if conditions such as acid adaptation and pathogen strain influence ultrasound efficacy, if the technology is to be adopted by industry. 相似文献12.
目的:优化食用菌的加工工艺,评估其作为风味剂在食品工业中的应用潜力。方法:使用离子色谱仪和高效液相色谱仪对样品的非挥发性滋味成分含量进行测定,并通过等鲜浓度值来评估样品的鲜味;运用GC-MS、电子鼻和电子舌来检测样本中的挥发性香气成分,观察纳米粉碎前后的微观结构,并进行增鲜减盐试验。结果:纳米粉碎后,大球盖菇和双孢蘑菇的鲜味氨基酸、呈味核苷酸和有机酸含量显著增加,挥发性化合物的种类和数量显著增加,而纳米粉碎对草菇的风味成分影响较小;电子舌结果表明,经纳米粉碎的样品在鲜味和咸味方面均有明显提升,加入0.5%纳米粉碎双孢蘑菇后能够增加0.28%的盐浓度,同时其鲜味值提高了145%。结论:纳米粉碎技术能够提高食品的口感,在食品制造业中可以将纳米粉碎后的食用菌粉作为替代盐和风味增强剂的有效选择。 相似文献
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The antibacterial action of thymol and cymene on two Bacillus cereus strains (INRA-AVTZ415 and INRA-AVZ421) was studied. Thymol and cymene alone showed higher antibacterial effect on the growth of B. cereus in BHI at 30 °C, both in lag and in exponential growth phases, with increasing added concentrations. However, the two strains tested showed different sensitivity to these natural compounds. When thymol and cymene were applied simultaneously, it resulted in a greater antibacterial effect than when the compounds were applied separately. The synergistic behaviour of both compounds was also shown for lag phase B. cereus INRA-AVZ421 cells in food substrates (carrot juice, chicken soup and pumpkin cream) at 30 °C, though a clear food-related effect was observed. This study indicates the potential use of thymol and cymene applied simultaneously for preservation of minimally processed foods. 相似文献
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清洁化制革生产工艺的研究是建立在毁毛废液、复灰废液和铬鞣废液循环利用研究的基础上,它的目的是建立一套既能保证成革质量,又可大大减轻生产污染的制革工艺。本部分报道了清洁化工艺连续执行过程毁毛废液、复灰废液及铬鞣废液各成分含量的变化;并从微观组织学及革的理化指标角度比较了清洁化工艺与基本工艺的成革质量。实验证明该研究达到了预定目标。 相似文献
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休闲食品伴随人们生活方式和食品科技及产业的发展得到迅速发展,已成为人们日常生活中不可缺失的部分。休闲食品行业的快速发展除了归功于政策推动,极为重要的发展动力还源于支撑整个行业前进的食品加工技术,其中包括膨化技术、挤压技术、油炸技术、脱水干燥技术等。这些关键的加工技术在近年来也发生了极大的变化,呈现出复合化的趋势,这些改变克服了很多以往加工中的缺点,拓宽了原料选择范围,丰富了产品种类,优化了产品质量。本文综述了休闲食品中重要的膨化技术、挤压技术、油炸技术、脱水干燥技术的应用及其研究进展,意在推动休闲食品创新发展的步伐。 相似文献
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二氧化碳(CO2)是一种温室气体,被认为是导致全球温度升高(也称为全球变暖)的主要因素.到2040年,全球能源需求预计将比2019年增长30%,而化石燃料仍将是满足这些需求的主要能源(预计占比74%),其直接燃烧所排放的CO2量也将持续增长.目前全球CO2总利用量低于2亿吨/年,与每年320亿吨以上的CO2排放量相比,... 相似文献
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