共查询到20条相似文献,搜索用时 57 毫秒
1.
Ming LiuAuthor Vitae Yangping WenAuthor Vitae Dong LiAuthor Vitae Ruirui YueAuthor VitaeJingkun XuAuthor Vitae Haohua HeAuthor Vitae 《Sensors and actuators. B, Chemical》2011,159(1):277-285
In this paper, a stable sandwich-type amperometric biosensor based on poly(3,4-ethylenedioxythiophene) (PEDOT)-single walled carbon nanotubes (SWCNT)/ascorbate oxidase (AO)/Nafion films for detection of l-ascorbic acid (AA) was successfully developed. PEDOT-SWCNT nanocomposite and Nafion films were used as inner and outer films, respectively. AO was immobilized between these two films. The PEDOT-SWCNT nanocomposite films were characterized by electrochemical impedance spectroscopy and scanning electron microscopy. The influence of detection potential and temperature on the biosensor performance was examined in detail. Despite the multilayer configuration, the biosensor exhibited a relatively fast response (less than 10 s) and a linear range from 1 μM to 18 mM (a correlation coefficient of 0.9974). The sensitivity of the biosensor was found to be 28.5 mA M−1 cm−2. Its experimental detection limit was 0.7 μM (S/N = 3) and the apparent Michaelis-Menten constant (Km) was calculated to be 18.35 mM. Moreover, the biosensor exhibited good anti-interferent ability and excellent long-term stability. All the results showed that such sandwich-type PEDOT-SWCNT/AO/Nafion films could provide a promising platform for the biosensor designs for AA detection. 相似文献
2.
E.A. DontsovaAuthor VitaeY.S. ZeifmanAuthor Vitae I.A. BudashovAuthor VitaeA.V. EremenkoAuthor Vitae S.L. KalnovAuthor VitaeI.N. KurochkinAuthor Vitae 《Sensors and actuators. B, Chemical》2011,159(1):261-270
This paper presents the amperometric biosensor that determines choline and cholinesterase activity using a screen printed graphite electrode. In order to detect H2O2 we have blanket modified the electrode material with manganese dioxide nanoparticles layer. Using layer-by-layer technique on the developed hydrogen peroxide sensitive electrode surface choline oxidase was incorporated into the interpolyelectrolyte nanofilm. Its ability to serve as a detector of choline in bulk analysis and cholinesterase assay was investigated. We examined the interferences from red-ox species and heavy metals in the blood and in the environmental sample matrixes. The sensor exhibited a linear increase of the amperometric signal at the concentration of choline ranging from 1.3 × 10−7 to 1.0 × 10−4 M, with a detection limit (evaluated as 3σ) of 130 nM and a sensitivity of 103 mA M−1 cm−2 under optimized potential applied (480 mV vs. Ag/AgCl). The biosensor retained its activity for more than 10 consecutive measurements and kept 75% of initial activity for three weeks of storage at 4 °C. The R.S.D. was determined as 1.9% for a choline concentration of 10−4 M (n = 10) with a typical response time of about 10 s. The developed choline biosensor was applied for butyrylcholinesterase assay showing a detection limit of 5 pM (3σ). We used the biosensor to develop the cholinesterase inhibitor assay. Detection limit for chlorpyrifos was estimated as 50 pM. 相似文献
3.
Xiaoyan JiangAuthor VitaeYanhui WuAuthor Vitae Xuyan MaoAuthor VitaeXiujun CuiAuthor Vitae Liande ZhuAuthor Vitae 《Sensors and actuators. B, Chemical》2011,153(1):158-163
This article reports a new amperometric glucose biosensor based on ordered mesoporous carbon (OMC) supported platinum nanoparticles (Pt/OMC) modified electrode. The Pt/OMC nanocomposite modified electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of H2O2 as well. This feature allowed us to use it as bioplatform on which glucose oxidase (GOD) was immobilized by entrapment in electropolymerized pyrrole film for the construction of the glucose biosensor. The biosensor showed good analytical performances in terms of low detection (0.05 mM), high sensitivity (0.38 μA/mM) and wide linear range (0.05-3.70 mM). In addition, the effects of pH value, applied potential, electroactive interference and the stability of the biosensor were discussed. The applicability to blood analysis was also evaluated. 相似文献
4.
C.S. PundirAuthor Vitae Nidhi ChauhanAuthor VitaeRajneeshAuthor Vitae Manjeet VermaAuthor VitaeRaviAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(2):796-803
An amperometric oxalate biosensor using nanohybrid film of multi-walled carbon nanotubes (MWCNTs) and gold colloidal nanoparticles (GNPs) via carbodiimide chemistry by forming amide linkages between carboxylic acid groups on the CNTs and amine residues of cysteamine self-assembled monolayer (SAM) has been prepared. The c-MWCNTs were immobilized on the gold (Au) electrode and characterized by FTIR. The morphologies of the c-MWCNT/Au and GNPs/MWCNT/Au electrodes were investigated by scanning electron microscopy (SEM) and the electrochemical performance of the Au, c-MWCNT/Au and GNPs/c-MWCNT/Au electrodes were also studied amperometrically. The Cl− and NO3− insensitive oxalate oxidase from grain sorghum was finally immobilized on this electrode. The influence of pH, temperature and oxalate concentration on electrode activity was studied. The electrode showed optimum response within 7 s. The electrocatalytic response showed a linear dependence on the oxalic acid concentration ranging from 1 to 800 μM with a detection limit of 1 μM. The Km value for the oxalic acid sensor was 444.44 μM. The enzyme electrode retained 30% of its initial activity after 5 months, when stored at 4 °C. The electrode was employed for measurement of oxalic acid in serum, urine and foodstuffs. 相似文献
5.
6.
Xue YanAuthor VitaeXiangjie BoAuthor Vitae Liping GuoAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(2):837-842
In this work, an electrochemical sensor based on ordered mesoporous carbon (OMC) for the amperometric detection of isoniazid was developed. OMC was dispersed in a solution of Nafion, and the suspension was modified onto the surface of glassy carbon (GC) electrode. Cyclic voltammetry and amperometry were used to investigate the electrochemical behaviors of isoniazid on Nafion-OMC modified electrode (Nafion-OMC/GC). The results indicate that OMC can facilitate the electrochemical oxidation of isoniazid with a great decrease of overpotential in pH 7.0 phosphate buffer solution. The proposed biosensor provides excellent performance towards the determination of isoniazid with a high sensitivity of 0.031 μA/μM, a low detection limit of 8.4 × 10−8 M and wide linear range from 1.0 × 10−7 M to 3.7 × 10−4 M at +0.20 V vs. Ag/AgCl. The method was successfully applied to the determination of isoniazid tablets with satisfying results. All the results suggest that Nafion-OMC/GC electrode is a potential candidate for a stable and efficient electrochemical sensor to detect isoniazid. 相似文献
7.
P. SalazarAuthor Vitae R.D. O’NeillAuthor VitaeM. MartínAuthor Vitae R. RocheAuthor VitaeJ.L. González-MoraAuthor Vitae 《Sensors and actuators. B, Chemical》2011,152(2):137-143
Results of experiments in vitro and in vivo, using an amperometric glucose microbiosensor based on a Prussian Blue (PB) modified carbon fiber electrode with very low dimensions (∼10 μm diameter), are presented. The electrocatalytic properties of the PB film enable detection of an enzymatic by-product (H2O2) at a very low applied potential: 0.0 V against SCE. The main steps during glucose microbiosensor construction were examined by cyclic voltammetry and electrochemical impedance spectroscopy. Excellent selectivity of the glucose microbiosensors against a large number of physiological interference compounds is demonstrated. Finally, microbiosensor responses during intraperitoneal injection, local infusion and local electrical stimulation showed sufficient sensitivity and stability to monitor multi-phasic and reversible changes in brain ECF glucose levels during physiological experiments, illustrating the excellent properties and utility of this biosensor design in the neurosciences. 相似文献
8.
A glucose oxidase immobilization platform for glucose biosensor using ZnO hollow nanospheres 总被引:1,自引:0,他引:1
Bin FangAuthor Vitae Cuihong ZhangAuthor VitaeGuangfeng WangAuthor Vitae Meifang WangAuthor Vitae Yulan JiAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(1):304-310
A good route (template-directed synthetic route) for the fabrication of ZnO hollow nanospheres (ZnO-HNSPs) was proposed. ZnO hollow nanosphere is a wonderful platform to immobilize glucose oxidase for glucose biosensor owing to the high specific surface area and high isoelectric point (IEP). Along with nafion and glucose oxidase (GOD), a glucose sensor was designed. Nafion/ZnO-HNSPs/GOD/GCE displays higher catalytic activity toward the glucose oxidation than Nafion/ZnO nano-Flowers/GOD/GCE. Linear response was obtained over a concentration range from 5.0 × 10−3 mM to 13.15 mM with a detection limit of 1.0 μM (S/N = 3), and the sensitivity was 65.82 μA/(mM cm2). Satisfyingly, the Nafion/ZnO-HNSPs/GOD/GCE could effectively avoid the interferences from the common interfering species such as uric acid (UA), ascorbic acid (AA), dopamine (DA) and fructose. The Nafion/ZnO-HNSPs/GOD modified electrode allows high sensitivity, excellently selective, stable, and fast amperometric sensing of glucose and thus is promising for the future development of glucose sensors. 相似文献
9.
Qingxiang WangAuthor Vitae Bin ZhangAuthor VitaeXiaoqiang LinAuthor Vitae Wen WengAuthor Vitae 《Sensors and actuators. B, Chemical》2011,156(2):599-605
A label-free DNA biosensor for hybridization detection of short DNA species related to the transgenic plants gene fragment of cauliflower mosaic virus (CaMV) 35S promoter was developed in this paper. The nanocomposite containing chitosan (CS) and mutiwalled carbon nanotubes (MWNTs) was first coated on a glassy carbon electrode. Then a highly reactive dialdehyde reagent of glutaraldehyde (GTD) was applied as an arm linker to covalently graft the 5′-amino modified probe DNA to the CS-MWNTs surface via the facile aldehyde-ammonia condensation reaction. The hybridization capacity of the developed biosensor was monitored with electrochemical impedance spectroscopy (EIS) using [Fe(CN)6]3−/4− as an indicating probe, and the experimental results showed that the biosensor had fast hybridization rate and low background interference. A wide dynamic detection range (1.0 × 10−13-5 × 10−10 M) and a low detection limit (8.5 × 10−14 M) were achieved for the complementary sequence. In addition, the hybridization specificity experiments showed that the sensing system can accurately discriminate complementary sequence from mismatch and noncomplementary sequences. 相似文献
10.
Maria D.L. OliveiraAuthor VitaeDulcinéia S.P. AbdallaAuthor Vitae Daniel F. GuilhermeAuthor VitaeTanize E.S. FaulinAuthor Vitae Cesar A.S. AndradeAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(2):775-781
Monoclonal antibodies (MAb) have been commonly applied to measure LDL in vivo and to characterize modifications of the lipids and apoprotein of the LDL particles. The electronegative low density lipoprotein (LDL−) has an apolipoprotein B-100 modified at oxidized events in vivo. In this work, a novel LDL− electrochemical biosensor was developed by adsorption of anti-LDL− MAb on an (polyvinyl formal)-gold nanoparticles (PVF-AuNPs)-modified gold electrode. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the recognition of LDL−. The interaction between MAb-LDL− leads to a blockage in the electron transfer of the [Fe(CN)6]4−/K4[Fe(CN)6]3− redox couple, which may could result in high change in the electron transfer resistance (RCT) and decrease in the amperometric responses in CV analysis. The compact antibody-antigen complex introduces the insulating layer on the assembled surface, which increases the diameter of the semicircle, resulting in a high RCT, and the charge transferring rate constant κ0 decreases from 18.2 × 10−6 m/s to 4.6 × 10−6 m/s. Our results suggest that the interaction between MAb and lipoprotein can be quantitatively assessed by the modified electrode. The PVF-AuNPs-MAb system exhibited a sensitive response to LDL−, which could be used as a biosensor to quantify plasmatic levels of LDL−. 相似文献
11.
Sayed A.M. MarzoukAuthor Vitae Jody D. HaddowAuthor VitaeAmr AminAuthor Vitae 《Sensors and actuators. B, Chemical》2011,157(2):647-653
In the present paper, we continue to utilize the sequential action of sialic acid aldolase and pyruvate oxidase to construct improved sialic acid (SA) amperometric biosensors and immobilized enzyme reactors (IERs). The enzymes were co-immobilized using glutaraldehyde crosslinking at the surface of a platinum disc electrode and by covalent attachment to the modified controlled pore glass beads packed in stainless steel columns to prepare the SA biosensors and IERs, respectively. Two different configurations were adapted in the present work to produce the first report on flow-injection amperometric determination of SA. In the first configuration, the SA biosensor was used as a detector in a flow injection setup. In the second configuration, an IER was used in conjunction with a flow-through amperometric cell equipped with a Pt disc electrode polarized at 0.7 V vs Ag/AgCl for the anodic detection of the produced hydrogen peroxide. We also report a simple way to control the temperature in the flow injection setups. The FI determination of SA based on IER was found to be more superior and showed linear dependence of the FI peak heights on SA concentration in the range of 0.002-5.0 mM (r2 = 0.9997), RSD of 1.1% at 250 μM SA and a sample throughput of 35 sample h−1. 相似文献
12.
Yongyan TanAuthor VitaeJinqing KanAuthor Vitae Shengqi LiAuthor Vitae 《Sensors and actuators. B, Chemical》2011,152(2):285-291
A novel amperometric biosensor for the determination of catechol was developed accordingly to the electrochemical template procedure. The optimum fabricating conditions of the biosensor were studied. The resulting biosensor with the limit of less than 0.05 μM can be used for detection of catechol in the linear range of 2.5-140 μM. The maximum response current (Imax) and the Michaelis-Menten constant (k′m) are 3.08 μA and 77.52 μM, respectively. The activation energy (Ea) of the polyphenol oxidase (PPO) catalytic reaction is 25.56 kJ mol−1 in the B-R buffer. The stability of the PANI-CA biosensor fabricated with the electrochemical template process (retains 86% of the original activity after four months) is much higher than that fabricated with one-step and two-step processes (retains 75% of the original activity after four months). The effects of potential and pH on the response current of the biosensor are also described. 相似文献
13.
Xiaojun ChenAuthor Vitae Jinwei ZhuAuthor VitaeZixuan ChenAuthor Vitae Chenbin XuAuthor VitaeYan WangAuthor Vitae Cheng YaoAuthor Vitae 《Sensors and actuators. B, Chemical》2011,159(1):220-228
The magnetic core-shell Au-Fe3O4@SiO2 nanocomposite was prepared by layer-by-layer assembly technique and was used to fabricate a novel bienzyme glucose biosensor. Glucose oxidase (GOD) and horseradish peroxidase (HRP) were simply mixed with Au-Fe3O4@SiO2 nanocomposite and cross-linked on the ITO magnetism-electrode with nafion (Nf) and glutaraldehyde (GA). The modified electrode was designated as Nf-GOD-HRP/Au-Fe3O4@SiO2/ITO. The effects of some experimental variables such as the pH of supporting electrolyte, enzyme loading, the concentration of the mediator methylene blue (MB) and the applied potential were investigated. The electrochemical behavior of the biosensor was studied using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and chronoamperometry. Under the optimized conditions, the biosensor showed a wide dynamic range for the detection of glucose with linear ranges of 0.05-1.0 mM and 1.0-8.0 mM, and the detection limit was estimated as 0.01 mM at a signal-to-noise ratio of 3. The biosensor exhibited a rapid response, good stability and anti-interference ability. Furthermore, the biosensor was successfully applied to detect glucose in human serum samples, showing acceptable accuracy with the clinical method. 相似文献
14.
Shichao HuiAuthor VitaeJian ZhangAuthor Vitae Xuejiao ChenAuthor VitaeHuhua XuAuthor Vitae Dianfei MaAuthor VitaeYanli LiuAuthor Vitae Bairui TaoAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(2):592-597
An amperometric glucose sensor based on Pd-Ni/SiNW electrode has been investigated. The silicon nanowire (SiNW) electrodes were first fabricated by chemical etching, and then nickel and palladium particles were deposited onto the surfaces of SiNWs via electroless co-plating technique followed by annealing in nitrogen atmosphere at 350 °C for 300 s. The morphology of Pd-Ni/SiNW electrode was characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD). The sensor performance was characterized by cyclic voltammetry (CV) and fixed potential amperometry techniques. In 0.1 M KOH alkaline medium with different glucose concentrations, the sensor shows an excellent sensitivity of 190.72 μA mM−1 cm−2 with the detection limit (S/N ratio = 3) of 2.88 μM. And it also exhibits superior anti-interference properties to the species including ascorbic acid (AA), uric acid (UA) and 4-acetamidophenol (AP). All results demonstrate that this Pd-Ni/SiNW electrode is a candidate with great potential for glucose detection. 相似文献
15.
Veronica Paz ZaniniAuthor Vitae Beatriz López de MishimaAuthor VitaeVelia SolísAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(1):75-80
A biosensor based on the immobilization of lactate oxidase (LOx) on a glassy carbon electrode modified with laponite/chitosan hydrogels for the quantification of l-lactate in alcoholic beverages and dairy products is presented. Ferrocene-methanol (FcMe) is used as artificial mediator. The purpose of this work is to determine the best hydrogel composition from the analytical point of view. The characterization of the hydrogels was carried out by CV, amperometry and EIS. According to permeabilities and charge transfer resistances for ferrocyanide (used as molecular probe) as well as the enzymatic behavior of the enzyme for l-lactate, the best laponite/chitosan mass ratio found was 25/50. The distinct features of the bioelectrode are its long stability, its ability to reject or minimize most interferents including ascorbic acid, and its excellent analytical response, which allowed the reduction of the enzyme content below 0.5 U, for a sensitivity of (0.326 ± 0.003) A cm−2 M−1, with a time response lower than 5 s and a detection limit of (3.8 ± 0.2) × 10−6 M. Our l-lactate biosensor was validated by comparison with a standard spectroscopic method. 相似文献
16.
R. ThangamuthuAuthor VitaeYu-Ching PanAuthor Vitae Shen-Ming ChenAuthor Vitae 《Sensors and actuators. B, Chemical》2011,151(2):377-383
The present work describes the electrocatalytic behavior of phosphotungstate-doped glutaraldehyde-cross-linked poly-l-lysine (PLL-GA-PW) film electrode towards reduction of hydrogen peroxide (H2O2) in acidic medium. The modified electrode was prepared by means of electrostatically trapping the phosphotungstate anion into the cationic PLL-GA coating on glassy carbon electrode. The PLL-GA-PW film electrode showed excellent electrocatalytic activity towards H2O2 reduction in 0.1 M H2SO4. Under the optimized conditions, the electrochemical sensor exhibited a linear response for H2O2 concentration over the range 2.5 × 10−6 to 6.85 × 10−3 M with a sensitivity of 1.69 μA mM−1. The curvature in the calibration curve at high concentration is explained in terms of Michaelis-Menten (MM) saturation kinetics, and the kinetics parameters calculated by three different methods were compared. The PLL-GA-PW film electrode did not respond to potential interferents such as dopamine, ascorbic acid and uric acid. This unique feature of PLL-GA-PW film electrode allowed selective determination of H2O2. Finally, the proposed electrochemical sensor was successfully applied to determine H2O2 in commercially available antiseptic solution and soft-contact lenses cleaning solution and the method has been validated using independent estimation by classical potassium permanganate titration method. Major advantages of the method are simple electrode fabrication, stability and high selectivity towards hydrogen peroxide. 相似文献
17.
Kuo-Chiang LinAuthor VitaeChien-Yu YinAuthor Vitae Shen-Ming ChenAuthor Vitae 《Sensors and actuators. B, Chemical》2011,157(1):202-210
An electrochemical biosensor for determination of hydrogen peroxide (H2O2) has been developed by the hybrid film of poly(methylene blue) and FAD (PMB/FAD). The PMB/FAD hybrid film was performed in PBS (pH 7) containing methylene blue and FAD by cyclic voltammetry. Repeatedly scanning potential range of −0.6-1.1 V, FAD was immobilized on the electrode surface by electrostatic interaction while methylene blue was electropolymerized on electrode surface. This modified electrode was found surface confined and pH dependence. It showed good electrocatalytic reduction for H2O2, KBrO3, KIO3, and NaClO as well as electrocatalytic oxidation for NADH. At an applied potential of −0.45 V vs. Ag/AgCl, the sensor showed a rapid and linear response to H2O2 over the range from 0.1 μM to 960 μM, with a detection limit of 0.1 μM and a significant sensitivity of 1109 μA mM−1 cm−2 (S/N = 3). It presented excellent stability at room temperature, with a variation of response current less than 5% over 30 days. 相似文献
18.
Lihong LeiAuthor Vitae Zhijun CaoAuthor VitaeQingji XieAuthor Vitae Yingchun FuAuthor VitaeYueming TanAuthor Vitae Ming MaAuthor VitaeShouzhuo YaoAuthor Vitae 《Sensors and actuators. B, Chemical》2011,157(1):282-289
We report on the electrodeposition of a 3-aminopropyltriethoxysilane-chitosan (APTES-CS) hybrid gel film for in situ immobilization of glucose oxidase (GOx) on an Au or platinized Au (Ptnano/Au) electrode for biosensing of glucose. Controllable electroreduction of p-benzoquinone is used to lift the electrode-surface pH for the GOx-APTES-CS codeposition, which was monitored by an electrochemical quartz crystal microbalance. The fabrication procedures of the biosensor and the parameters influencing the biosensing performance were optimized. The prepared porous GOx-APTES-CS/Ptnano/Au and GOx-APTES-CS/Au electrodes can be used to detect the enzymatically generated H2O2 at 0.5 and 0.7 V vs SCE, respectively. The enzyme electrodes exhibited linear responses to glucose concentration from 0.2 μM to 8.2 mM (R = 0.998, at Ptnano/Au substrate) and from 0.2 μM to 5.5 mM (R = 0.998, at Au substrate), with current sensitivities of 69.5 (Ptnano/Au) and 65 (Au) μA mM−1 cm−2, respectively, and a detection limit of 0.2 μM (S/N = 3) was achieved for each electrode. The response time was less than 5 (Ptnano/Au) or 8 (Au) s. It is striking that the enzyme electrodes remained their initial response sensitivity after storage for 5 (Au) and >6 (Ptnano/Au) months in 0.10 M PBS (pH 7.0) at 4 °C. 相似文献
19.
Jing LiAuthor VitaeHuaqing XieAuthor Vitae Lifei ChenAuthor Vitae 《Sensors and actuators. B, Chemical》2011,153(1):239-245
A highly sensitive hydrazine sensor was developed based on the electrodeposition of gold nanoparticles onto the choline film modified glassy carbon electrode (GNPs/Ch/GCE). The electrochemical experiments showed that the GNPs/Ch film exhibited a distinctly higher activity for the electro-oxidation of hydrazine than GNPs with 3.4-fold enhancement of peak current. The kinetic parameters such as the electron transfer coefficient (α) and the rate of electron exchange (k) for the oxidation of hydrazine were determined. The diffusion coefficient (D) of hydrazine in solution was also calculated by chronoamperometry. The sensor exhibited two wide linear ranges of 5.0 × 10−7-5.0 × 10−4 and 5.0 × 10−4-9.3 × 10−3 M with the detection limit of 1.0 × 10−7 M (s/n = 3). The proposed electrode presented excellent operational and storage stability for the determination of hydrazine. Moreover, the sensor showed outstanding sensitivity, selectivity and reproducibility properties. All the results indicated a good potential application of this sensor in the detection of hydrazine. 相似文献
20.
Jing WangAuthor VitaeDaqian SongAuthor Vitae Liying WangAuthor VitaeHua ZhangAuthor Vitae Hanqi ZhangAuthor VitaeYing SunAuthor Vitae 《Sensors and actuators. B, Chemical》2011,157(2):547-553
A novel method for detecting human IgG is reported, which is based on Au/Ag alloy nanocomposites for amplifying surface plasmon resonance response. Au/Ag alloy nanocomposites were characterized in detail by transmission electron microscopy (TEM), UV-vis absorption spectroscopy and X-ray photoelectron spectroscopy (XPS). Covalent immobilization of about 24 nm diameter of Au/Ag alloy nanocomposites on the Au film results in a large shift in resonance wavelength, which is due to the increase of the thickness of the sensing membrane, high dielectric constant of Au/Ag nanoparticles, and electromagnetic coupling between Au/Ag alloy nanocomposites and Au film. The SPR biosensor based on Au/Ag alloy nanocomposites exhibits a satisfactory response for human IgG in the concentration range of 0.15-40.00 μg mL−1. While the biosensor based on Au nanoparticles shows a response in the concentration range of 0.30-20.00 μg mL−1 and the biosensor based on Au film shows a response for human IgG in the concentration range of 1.25-20.00 μg mL−1. 相似文献