Reducing life cycle greenhouse gas emissions of corn ethanol by integrating biomass to produce heat and power at ethanol plants

A life-cycle assessment (LCA) of corn ethanol was conducted to determine the reduction in the life-cycle greenhouse gas (GHG) emissions for corn ethanol compared to gasoline by integrating biomass fuels to replace fossil fuels (natural gas and grid electricity) in a U.S. Midwest dry-grind corn ethanol plant producing 0.19 hm³ y⁻¹ of denatured ethanol. The biomass fuels studied are corn stover and ethanol co-products [dried distillers grains with solubles (DDGS), and syrup (solubles portion of DDGS)]. The biomass conversion technologies/systems considered are process heat (PH) only systems, combined heat and power (CHP) systems, and biomass integrated gasification combined cycle (BIGCC) systems. The life-cycle GHG emission reduction for corn ethanol compared to gasoline is 38.9% for PH with natural gas, 57.7% for PH with corn stover, 79.1% for CHP with corn stover, 78.2% for IGCC with natural gas, 119.0% for BIGCC with corn stover, and 111.4% for BIGCC with syrup and stover. These GHG emission estimates do not include indirect land use change effects. GHG emission reductions for CHP, IGCC, and BIGCC include power sent to the grid which replaces electricity from coal. BIGCC results in greater reductions in GHG emissions than IGCC with natural gas because biomass is substituted for fossil fuels. In addition, underground sequestration of CO₂ gas from the ethanol plant’s fermentation tank could further reduce the life-cycle GHG emission for corn ethanol by 32% compared to gasoline.     

Incorporating uncertainty analysis into life cycle estimates of greenhouse gas emissions from biomass production

Before further investments are made in utilizing biomass as a source of renewable energy, both policy makers and the energy industry need estimates of the net greenhouse gas (GHG) reductions expected from substituting biobased fuels for fossil fuels. Such GHG reductions depend greatly on how the biomass is cultivated, transported, processed, and converted into fuel or electricity. Any policy aiming to reduce GHGs with biomass-based energy must account for uncertainties in emissions at each stage of production, or else it risks yielding marginal reductions, if any, while potentially imposing great costs.This paper provides a framework for incorporating uncertainty analysis specifically into estimates of the life cycle GHG emissions from the production of biomass. We outline the sources of uncertainty, discuss the implications of uncertainty and variability on the limits of life cycle assessment (LCA) models, and provide a guide for practitioners to best practices in modeling these uncertainties. The suite of techniques described herein can be used to improve the understanding and the representation of the uncertainties associated with emissions estimates, thus enabling improved decision making with respect to the use of biomass for energy and fuel production.     

Life-cycle greenhouse gas emissions and energy balances of sugarcane ethanol production in Mexico

The purpose of this work was to estimate GHG emissions and energy balances for the future expansion of sugarcane ethanol fuel production in Mexico with one current and four possible future modalities. We used the life cycle methodology that is recommended by the European Renewable Energy Directive (RED), which distinguished the following five system phases: direct Land Use Change (LUC); crop production; biomass transport to industry; industrial processing; and ethanol transport to admixture plants. Key variables affecting total GHG emissions and fossil energy used in ethanol production were LUC emissions, crop fertilization rates, the proportion of sugarcane areas that are burned to facilitate harvest, fossil fuels used in the industrial phase, and the method for allocation of emissions to co-products. The lower emissions and higher energy ratios that were observed in the present Brazilian case were mainly due to the lesser amount of fertilizers applied, also were due to the shorter distance of sugarcane transport, and to the smaller proportion of sugarcane areas that were burned to facilitate manual harvest. The resulting modality with the lowest emissions of equivalent carbon dioxide (CO_2e) was ethanol produced from direct juice and generating surplus electricity with 36.8 kgCO_2e/GJ_ethanol. This was achieved using bagasse as the only fuel source to satisfy industrial phase needs for electricity and steam. Mexican emissions were higher than those calculated for Brazil (27.5 kgCO_2e/GJ_ethanol) among all modalities. The Mexican modality with the highest ratio of renewable/fossil energy was also ethanol from sugarcane juice generating surplus electricity with 4.8 GJ_ethanol/GJ_fossil.     

Optimal household refrigerator replacement policy for life cycle energy,greenhouse gas emissions,and cost

Although the last decade witnessed dramatic progress in refrigerator efficiencies, inefficient, outdated refrigerators are still in operation, sometimes consuming more than twice as much electricity per year compared with modern, efficient models. Replacing old refrigerators before their designed lifetime could be a useful policy to conserve electric energy and greenhouse gas emissions. However, from a life cycle perspective, product replacement decisions also induce additional economic and environmental burdens associated with disposal of old models and production of new models. This paper discusses optimal lifetimes of mid-sized refrigerator models in the US, using a life cycle optimization model based on dynamic programming. Model runs were conducted to find optimal lifetimes that minimize energy, global warming potential (GWP), and cost objectives over a time horizon between 1985 and 2020. The baseline results show that depending on model years, optimal lifetimes range 2–7 years for the energy objective, and 2–11 years for the GWP objective. On the other hand, an 18-year of lifetime minimizes the economic cost incurred during the time horizon. Model runs with a time horizon between 2004 and 2020 show that current owners should replace refrigerators that consume more than 1000 kWh/year of electricity (typical mid-sized 1994 models and older) as an efficient strategy from both cost and energy perspectives.     

Hydrothermal pretreatment of switchgrass and corn stover for production of ethanol and carbon microspheres

Pretreatment of biomass is viewed as a critical step to make the cellulose accessible to enzymes and for an adequate yield of fermentable sugars in ethanol production. Recently, hydrothermal pretreatment methods have attracted a great deal of attention because it uses water which is a inherently present in green biomass, non-toxic, environmentally benign, and inexpensive medium. Hydrothermal pretreatment of switchgrass and corn stover was conducted in a flow through reactor to enhance and optimize the enzymatic digestibility. More than 80% of glucan digestibility was achieved by pretreatment at 190 °C. Addition of a small amount of K₂CO₃ (0.45-0.9 wt.%) can enhance the pretreatment and allow use of lower temperatures. Switchgrass pretreated at 190 °C only with water had higher internal surface area than that pretreated in the presence of K₂CO₃, but both the substrates showed similar glucan digestibility. In comparison to switchgrass, corn stover required milder pretreatment conditions. The liquid hydrolyzate generated during pretreatment was converted into carbon microspheres by hydrothermal carbonization, providing a value-added byproduct. The carbonization process was further examined by GC-MS analysis to understand the mechanism of microsphere formation.     

The inhomogeneity of corn stover and its effects on bioconversion

Compared with wood, corn stover (CS) is a low-value raw material. Traditionally, CS is used in whole. To develop a CS high-value utilization process, the inhomogeneity of CS and its effects on bioconversion were investigated. The results showed that the chemical compositions of parts from CS had a remarkable difference. The Standard Deviation of cellulose, hemicellulose, Klason lignin, ash of CS parts (leaf, shell, core, node) were 3.09, 9.29, 3.32 and 4.58, respectively. The percents of fiber cell, parenchyma cell, epidermis cell and vessel cell were 30%, 30%, 10%, 30% (in leaf), 50%, 20%, 25%, 5% (in shell), 30%, 60%, 10% and 0% (in core), respectively. The inhomogeneity of CS chemical compositions and cell types affects its enzymatic hydrolysis and fermentation performances. The cellulose enzymatic hydrolysis ratio of corn core at 48 h was 130% higher than the leaf. After 7 d solid state fermentation, the filter paper activity of shell fiber, leaf fiber, core fiber, shell mixed cells, leaf mixed cells and core mixed cells were 40.6, 62.9, 64.1, 67.3, 194.2 and 154.0 IU g⁻¹ dry medium, respectively. The differences proved that the whole utilization process was unsatisfactory and suggested the potential of CS fractionation. Based the results, a pilot scale CS fractionation process (CS- Steam explosion-Water washing-Mechanical fiber fractionation-fiber cell and miscellaneous cells) was tested and divided corn stover into fiber cell and miscellaneous cells in the ratio of 1:1 approximately. The study showed the essentiality of CS fractionation and feasibility of fractionation by a simple method.     

Life cycle assessment of greenhouse gas emissions of feedlot manure management practices: Land application versus gasification

Animal waste is an important source of anthropogenic GHG emissions, and in most cases, manure is managed by land application. Nevertheless, due to the huge amounts of manure produced annually, alternative manure management practices have been proposed, one of which is gasification, aimed to convert manure into clean energy-syngas. Syngas can be utilized to provide energy or power. At the same time, the byproduct of gasification, biochar, can be transported back to fields as a soil amendment. Environmental impacts are crucial in selecting the appropriate manure strategy. Therefore, GHG emissions during manure management systems (land application and gasification) were evaluated and compared by life cycle assessment (LCA) in our study. LCA is a universally accepted tool to determine GHG emissions associated with every stage of a system. Results showed that the net GHG emissions in land application scenario and gasification scenario were 119 and -643 kg CO₂-eq for one tonne of dry feedlot manure, respectively. Moreover, sensitive factors in the gasification scenario were efficiency of the biomass integrated gasification combined cycle (BIGCC) system and energy source of avoided electricity generation. Overall, due to the environmental effects of syngas and biochar, gasification of feedlot manure is a much more promising technique as a way to reduce GHG emissions than is land application.     

Production costs of potential corn stover harvest and storage systems

Corn stover has potential as a bioenergy feedstock in North America. We simulated production costs for stover harvest (three-pass and two-pass with baling or chopping, and single-pass with baling or chopping) and on-farm storage (outdoor and indoor bales, outdoor wrapped bales, and chopped stover in bags, bunks, or piles). For three- and two-pass harvest, chopping was 33–45% more expensive than baling. For baling and chopping, two-pass harvest was 25% cheaper than three-pass. Single-pass chopping harvests were on average 42% cheaper than three-pass or two-pass chopping. Single-pass baling was cheaper (4–31%) than multi-pass baling at low rates of stover collection, but more expensive (1–39%) at high rates of collection. For bales, outdoor storage of wrapped bales was cheapest. Outdoor, unwrapped bale storage, even with 12% dry matter loss, was cheaper than indoor storage. For chopped stover, storage in bags was always cheapest, followed by piles, and then bunkers. With harvest and storage together, there were four least cost systems: single-pass, ear-snap baling with wrapped bale storage; single-pass chopping with silage bag storage; and two-pass baling with wrapped-bale storage. A second group of harvest/storage systems was 25% more expensive, including single-pass, whole-plant baling with wrapped-bale storage; two-pass chopping with silage-bag storage; and three-pass baling with wrapped-bale storage. The three-pass chop harvest with silage bag storage was most expensive. Our analysis suggests all harvest and farm storage systems have tradeoffs and several systems can be economically and logistically viable.     

Life cycle analysis of energy use and greenhouse gas emissions for road transportation fuels in China

Life cycle analysis is considered to be a valuable tool for decision making towards sustainability. Life cycle energy and environmental impact analysis for conventional transportation fuels and alternatives such as biofuels has become an active domain of research in recent years. The present study attempts to identify the most reliable results to date and possible ranges of life cycle fossil fuel use, petroleum use and greenhouse gas emissions for various road transportation fuels in China through a comprehensive review of recently published life cycle studies and review articles. Fuels reviewed include conventional gasoline, conventional diesel, liquefied petroleum gas, compressed natural gas, wheat-derived ethanol, corn-derived ethanol, cassava-derived ethanol, sugarcane-derived ethanol, rapeseed-derived biodiesel and soybean-derived biodiesel. Recommendations for future work are also discussed.     

Life cycle greenhouse gas emissions analysis of catalysts for hydrotreating of fast pyrolysis bio-oil

Bio-oil from fast pyrolysis of biomass requires multi-stage catalytic hydroprocessing to produce hydrocarbon drop-in fuels. One process design currently in development involves fixed beds of ruthenium-based catalyst and conventional petroleum hydrotreating catalyst. As the catalyst is spent over time as a result of coking and other deactivation mechanisms, it must be changed out and replaced with fresh catalyst. A main focus of bio-oil upgrading research is increasing catalyst lifetimes to 1 year. Biofuel life cycle greenhouse gas (GHG) assessments typically ignore the impact of catalyst consumed during fuel conversion as a result of limited lifetime, representing a data gap in the analyses. To help fill this data gap, life cycle GHGs were estimated for two representative examples of fast pyrolysis bio-oil hydrotreating catalyst, NiMo/Al₂O₃ and Ru/C, and integrated into the conversion-stage GHG analysis. Life cycle GHGs are estimated at 5.5 kg CO₂-e/kg catalyst for NiMo/Al₂O₃. Results vary significantly for Ru/C, depending on whether economic or mass allocation methods are used. Life cycle GHGs for Ru/C are estimated at 80.4 kg CO₂-e/kg catalyst using economic allocation and 13.7 kg CO₂-e/kg catalyst using mass allocation. Contribution of catalyst consumption to total conversion-stage GHGs at 1-year catalyst lifetimes is 0.5% for NiMo/Al₂O₃ and 5% for Ru/C when economic allocation is used (1% for mass allocation). This analysis does not consider the use of recovered metals from catalysts and other wastes for catalyst manufacture and therefore these are likely to be conservative estimates compared to applications where a spent catalyst recycler can be used.     

Influence of driving patterns on life cycle cost and emissions of hybrid and plug-in electric vehicle powertrains

We compare the potential of hybrid, extended-range plug-in hybrid, and battery electric vehicles to reduce lifetime cost and life cycle greenhouse gas emissions under various scenarios and simulated driving conditions. We find that driving conditions affect economic and environmental benefits of electrified vehicles substantially: Under the urban NYC driving cycle, hybrid and plug-in vehicles can cut life cycle emissions by 60% and reduce costs up to 20% relative to conventional vehicles (CVs). In contrast, under highway test conditions (HWFET) electrified vehicles offer marginal emissions reductions at higher costs. NYC conditions with frequent stops triple life cycle emissions and increase costs of conventional vehicles by 30%, while aggressive driving (US06) reduces the all-electric range of plug-in vehicles by up to 45% compared to milder test cycles (like HWFET). Vehicle window stickers, fuel economy standards, and life cycle studies using average lab-test vehicle efficiency estimates are therefore incomplete: (1) driver heterogeneity matters, and efforts to encourage adoption of hybrid and plug-in vehicles will have greater impact if targeted to urban drivers vs. highway drivers; and (2) electrified vehicles perform better on some drive cycles than others, so non-representative tests can bias consumer perception and regulation of alternative technologies. We discuss policy implications.     

Aspen Plus simulation of biomass integrated gasification combined cycle systems at corn ethanol plants

Biomass integrated gasification combined cycle (BIGCC) systems and natural gas combined cycle (NGCC) systems are employed to provide heat and electricity to a 0.19 hm³ y⁻¹ (50 million gallon per year) corn ethanol plant using different fuels (syrup and corn stover, corn stover alone, and natural gas). Aspen Plus simulations of BIGCC/NGCC systems are performed to study effects of different fuels, gas turbine compression pressure, dryers (steam tube or superheated steam) for biomass fuels and ethanol co-products, and steam tube dryer exhaust treatment methods. The goal is to maximize electricity generation while meeting process heat needs of the plant. At fuel input rates of 110 MW, BIGCC systems with steam tube dryers provide 20–25 MW of power to the grid with system thermal efficiencies (net power generated plus process heat rate divided by fuel input rate) of 69–74%. NGCC systems with steam tube dryers provide 26–30 MW of power to the grid with system thermal efficiencies of 74–78%. BIGCC systems with superheated steam dryers provide 20–22 MW of power to the grid with system thermal efficiencies of 53–56%. The life-cycle greenhouse gas (GHG) emission reduction for conventional corn ethanol compared to gasoline is 39% for process heat with natural gas (grid electricity), 117% for BIGCC with syrup and corn stover fuel, 124% for BIGCC with corn stover fuel, and 93% for NGCC with natural gas fuel. These GHG emission estimates do not include indirect land use change effects.     

Good or bad bioethanol from a greenhouse gas perspective – What determines this?

The purpose of this study is to describe how the greenhouse gas (GHG) benefits of ethanol from agricultural crops depend on local conditions and calculation methods. The focus is mainly on the fuels used in the ethanol process and biogenic GHG from the soils cultivated. To ensure that “good” ethanol is produced, with reference to GHG benefits, the following demands must be met: (i) ethanol plants should use biomass and not fossil fuels, (ii) cultivation of annual feedstock crops should be avoided on land rich in carbon (above and below ground), such as peat soils used as permanent grassland, etc., (iii) by-products should be utilised efficiently in order to maximise their energy and GHG benefits and (iv) nitrous oxide emissions should be kept to a minimum by means of efficient fertilisation strategies, and the commercial nitrogen fertiliser utilised should be produced in plants which have nitrous oxide gas cleaning. Several of the current ethanol production systems worldwide fullfill the majority of these demands, whereas some production systems do not. Thus, the findings in this paper helps identifying current “good” systems, how today’s “fairly good” systems could be improved, and which inherent “bad” systems that we should avoid.     

Development status and life cycle inventory analysis of biofuels in Taiwan

This research conducted the life cycle inventory analyses of biofuels in Taiwan. The biofuels considered include bioethanol production from sugarcane as well as biodiesel production from soybean and rapeseed. Energy inputs and pollutant emission (including carbon dioxide) are the input/output items analyzed. Results obtained from the inventory analyses can be summarized as follows. Bioethanol production from per hectare sugarcane cropland is 5160 L (liters), meanwhile, 476 and 1012 L biodiesel can be produced from 1 ha of soybean and rapeseed, respectively. The energy input to produce a liter ethanol, a liter biodiesel produced from soybean and rapeseed are 1256, 9602 and 5191 kcal, respectively. Those energy inputs are still less than the energy content of ethanol or biodiesel. It can be concluded that there is a positive energy benefit in producing biofuels based on a comparison with the previous work. In addition, through their life cycle, 1478.4 kg CO₂ emission is generated from one hectare of soybean land and 2954.1 kg is generated from rapeseed land. Life cycle carbon dioxide emissions released from burning ethanol is 0.08 kg/LOE in contrast to 2.6 kg/LOE released from burning fossil gasoline.     

Comparative study on bio-hydrogen production from corn stover: Photo-fermentation,dark-fermentation and dark-photo co-fermentation

In this study, three different fermentation methods, such as photo-fermentation (PF), dark-fermentation (DF) and dark-photo co-fermentation (DPCF) for bio-hydrogen production from corn stover were compared in terms of hydrogen production, substrate consumption, by-products formation and energy conversion efficiency. A modified Gompertz model was applied to perform the kinetic analysis of hydrogen production. The maximum cumulative hydrogen yield of 141.42 mL·(g TS)⁻¹ was achieved by PF, DF with the minimum cumulative hydrogen yield of 36.08 mL· (g TS)⁻¹ had the shortest lag time of 4.33 h, and DPCF had the maximum initial hydrogen production rate of 1.88 mL· (g TS)⁻¹·h⁻¹ and maximum initial hydrogen content of 44.40%. The results also indicated PF was an acid-consuming process with a low total VFAs concentration level of 2.90–4.19 g·L⁻¹, DF was a process of VFAs accumulation with a maximum total VFAs concentration of 12.66 g·L⁻¹, and DPCF was a synergistic process in which the total VFAs concentration was significantly reduced and the hydrogen production efficiency was effectively improved compared with DF. The energy conversion efficiency of PF, DF and DPCF were 10.12%, 2.58% and 6.45%, respectively.     

Energy and greenhouse gas emission effects of corn and cellulosic ethanol with technology improvements and land use changes

Use of ethanol as a transportation fuel in the United States has grown from 76 dam³ in 1980 to over 40.1 hm³ in 2009 — and virtually all of it has been produced from corn. It has been debated whether using corn ethanol results in any energy and greenhouse gas benefits. This issue has been especially critical in the past several years, when indirect effects, such as indirect land use changes, associated with U.S. corn ethanol production are considered in evaluation. In the past three years, modeling of direct and indirect land use changes related to the production of corn ethanol has advanced significantly. Meanwhile, technology improvements in key stages of the ethanol life cycle (such as corn farming and ethanol production) have been made. With updated simulation results of direct and indirect land use changes and observed technology improvements in the past several years, we conducted a life-cycle analysis of ethanol and show that at present and in the near future, using corn ethanol reduces greenhouse gas emission by more than 20%, relative to those of petroleum gasoline. On the other hand, second-generation ethanol could achieve much higher reductions in greenhouse gas emissions. In a broader sense, sound evaluation of U.S. biofuel policies should account for both unanticipated consequences and technology potentials. We maintain that the usefulness of such evaluations is to provide insight into how to prevent unanticipated consequences and how to promote efficient technologies with policy intervention.     

Rheological properties of corn stover hydrolysate and photo-fermentation bio-hydrogen producing capacity under intermittent stirring

Rheological properties of substrates significantly affect energy consumption and hydrogen production potential in the process of hydrogen production with stirring. Hence, the rheological properties of corn stover hydrolysate with diverse concentrations and its hydrogen producing capacities under intermittent stirring conditions were investigated in this paper. The results showed that corn stover hydrolysate exhibited pseudo-plastic flow behavior at total solid (TS) of 2.76%–7.65%, and was well fitted by the Power law model. Among four intermittent stirring modes, intermittent stirring C2 (static time: stirring time is 2:1) obtained the highest hydrogen yield of 57.63 ± 1.75 mL g⁻¹ VS, which was 18.97% higher compared with static-culture. Moreover, the maximum hydrogen production rate of intermittent stirring C2 increased by 65.05% compared to continuous stirring. It's a feasible way to improve hydrogen production performance with proper intermittent stirring.     

Analysis of shaking effect on photo-fermentative hydrogen production under different concentrations of corn stover powder

High solid phase and easily congeal affect the mass transfer in the photo-fermentative biohydrogen production when taken straw biomass as substrate. Hence, oscillator was adopted to provide the shaking condition to enhance the mass transfer situation in this paper. Diverse shaking velocity (0, 80, 120 and 160 rpm) and substrate concentration (0, 2, 4, 6, 8 and 10 g) were studied, to evaluate the influence on the hydrogen yield capacity. The results showed that shaking could help to accelerate of gas release, shorten the fermentation time, and improve hydrogen production rate. Hydrogen yield was significantly enhanced at high substrate concentration under shaking condition. Highest hydrogen yield of 57.08 ± 0.83, 57.62 ± 1.37, 62.28 ± 0.84 mL/g-volatile solids (VS) were observed at shaking velocities of 80, 120 and 160 rpm with 6, 8 and 10 g corn stover powder, respectively. On the contrary, shaking significantly reduced the potential of hydrogen yield at a low substrate concentration, and the lower hydrogen yield obtained at the higher shaking velocity. As the lowest hydrogen yields of 27.68 ± 1.02 and 41.93 ± 0.40 mL/g VS were obtained at shaking velocity of 160 rpm with 2 and 4 g corn stover powder, respectively.     

Life cycle greenhouse gas emissions impacts of the adoption of the EU Directive on biofuels in Spain. Effect of the import of raw materials and land use changes

The objective of this paper is to evaluate the greenhouse gas (GHG) emissions impacts of the use of different alternative biofuels in passenger vehicles in Spain in order to meet EU biofuel goals. Different crop production alternatives are analysed, including the possible import of some raw materials. Availability of land for national production of the raw materials is analysed and indirect land use changes and associated GHG emissions are quantified.There are important differences in GHG emissions of biofuels depending on the raw material used and whether this is domestically produced or imported. Ethanol production using imported cereals and FAME production using domestic rapeseed have the highest GHG emissions per kilometre driven. Fatty acid methyl ester (FAME) production from sunflower has shown the lowest emissions. When taking into account the results of GHG emissions savings per hectare, these findings are somehow reversed. Production of ethanol and around 12% of FAME can be done domestically. The rest will need to be imported and will cause indirect land use change (ILUC). Therefore, ethanol production will not displace any land, whereas FAME production will displace some amounts of land. Calculated ILUC factors are 29%-34%. The additional GHG emissions due to these indirect land use changes are significant (67%-344% of life cycle GHG emissions).Standalone, the EU biofuel targets can have important benefits for Spain in terms of global warming emissions avoided. However, when considering the impact of land use change effects, these benefits are significantly reduced and can even be negative.     

Comparison of four different chemical pretreatments of corn stover for enhancing enzymatic digestibility

Four commonly used chemical pretreatment processes based on dilute acid, lime, aqueous ammonia steeping followed by dilute acid hydrolysis, and sodium hydroxide, were evaluated to provide comparative performance data. An obverse correlation between lignin removal and enzymatic digestibility of pretreated corn stover was observed. Compared with other three pretreatments, pretreatment of corn stover with 2% NaOH substantially increased the lignin removal and enhanced the accessibility and digestibility of cellulose. The hydrolysis yield of NaOH-pretreated corn stover reached 81.2% by 48 h at 8.0% substrate concentration and cellulase dosage of 20 FPU g⁻¹ substrate. Chemical analysis showed that the enzymatic hydrolysate from NaOH-pretreated corn stover contained higher content of fermentable sugars and less inhibitors, which is suitable for subsequent fermentation process to produce ethanol. The research results are meaningful in bioconversion and utilization of renewable lignocellulosic biomass.