谱域光学相干层析成像量化技术及其在生物组织定量分析中的应用

研究了谱域光学相干层析(SDOCT)成像系统的量化技术,通过量化OCT图像来获得生物组织内部的信息特征对组织光学散射特性进行定量分析.给出并讨论了单次散射模型,具有轴向点散射函数(PSF)的单次散射模型和多次散射模型,利用平均A-scan算法和非线性最小二乘法曲线拟合,研究不同浓度IntralipidTM溶液的散射特性.实验显示,IntralipidTM溶液的散射系数与浓度在1％～10％间基本呈线性关系,验证了具有PSF的单次散射模型比较适用于本文的谱域光学相干层析成像系统.利用该模型对小鼠的新鲜肝脏进行量化研究,得到小鼠新鲜肝脏在波长λ.为1550 nm时的散射系数为8.9 mm-1.本文的研究为该项技术今后在临床医学上对各种疾病的诊断和治疗奠定了基础.     

近红外显微图像的主成分法图像处理

介绍主成分分析算法在近红外显微图像分析中的应用,用该方法成功地提取出样品成分相关特征信息,并通过不同主成分的得分图像来描述样品的显微结构特征和特定化学成分分布。     

Image analysis of macroporous polystyrene sorbents using electron spectroscopic imaging to enhance mass contrast. The architectural relationship between polymer porosity and absorption capacity

We describe the microscopical analysis of two polystyrene divinylbenzene copolymers (PS-DVB) made using 20 and 55% (v/v) DVB cross-linker respectively, in which the hydrated structure of each polymeric material solvated in water is preserved by employing very rapid freezing and low-temperature freeze-drying techniques. Using the zero-loss mode of electron spectroscopic imaging to enhance the contrast of unstained polymer sections, we introduce some novel methods for the application of computer-assisted image analysis for routine examination of polymer pore structure. We also relate these parameters to the ability of the two polymers to absorb nanometre-sized particles—neutral colloidal gold particles and cationized ferritin. Both methods will be invaluable in the structural analysis of porous polymers, but particularly for comparative analyses of macroporous sorbents.