静电纺玉米醇溶蛋白纳米纤维及其药物缓释行为

利用静电纺丝法制备了玉米醇溶蛋白(Zein)、Zein/乙二醛交联纳米纤维,分别制得了负载不同质量药物的载药纳米纤维膜,并对纤维膜形态与结构进行了表征,研究了药物缓释行为。利用扫描电镜(SEM)对交联前后与负载药物后纳米纤维的形貌和直径分布进行了分析;强力测试结果表明,乙二醛交联后的Zein纳米纤维强度提高了近10倍;傅里叶变换红外光谱(FTIR)研究了Zein、乙二醛与药物之间的分子间作用力;紫外可见光(UV-Vis)光谱分析表明,乙二醛交联Zein载药纤维累积释放率比未交联的高且有一定的缓释效果。     

亲水性玉米醇溶蛋白的改性研究进展

玉米醇溶蛋白(Zein)是一种天然可再生、生物相容性好的植物大分子,其作为食品和药物递送载体已成为国内外研究热点。Zein的强疏水性限制了其应用,本文为此综述了亲水性Zein的改性研究进展,主要包括物理改性、化学改性和酶催化改性,以期为Zein的亲水改性研究及作为递送载体的应用研究提供参考。     

玉米醇溶蛋白的电纺研究进展

综述了玉米醇溶蛋白（Zein）的组成、性能以及应用情况,详细介绍了Zein在静电纺丝方面的研究。目前的研究表明：电纺Zein纳米纤维具有优良的生物相溶性、可降解性、降解产物无毒副作用等优点,且原料丰富,生产工艺简单,必将在生物医学领域具有广泛的应用前景。但必须在提高纤维的力学性能,开展生物实验等方面深入研究。     

缓香型玉米醇溶蛋白微胶囊乳液的制备及应用

为改善皮革的特殊气味并提升其应用性能,该研究以玉米醇溶蛋白（Zein）、艾叶香精（AAE）以及普兰尼克F127等为原料,通过反溶剂法制备缓香型玉米醇溶蛋白微胶囊（AAE@ZMs）乳液,再将AAE@ZMs乳液与成膜剂己内酰胺改性酪素复配后应用于皮革涂饰。结果表明,Zein与F127、AAE和戊二醛通过化学交联和氢键相互作用,同时F127和AAE可使Zein中的氨基酸重排并发生荧光猝灭;制得的AAE@ZMs乳液中微胶囊平均粒径约为298.6 nm且具有较好的分散性和稳定性;AAE@ZMs的包覆率和负载率分别可以达到73.2%和5.8%,在良溶剂中释放120 h时的AAE累积释放率约为83.1%。将AAE@ZMs应用于皮革涂饰后,涂饰革样中AAE的释放周期为6周,对金黄色葡萄球菌和大肠杆菌均具有较好的抑制效果,同时涂饰革样还展现出较好的机械性能。     

玉米醇溶蛋白用作药物输送系统载体的研究进展

玉米醇溶蛋白(Zein)是一种天然可食性植物大分子,具有可再生性、无毒性、两亲性及良好的生物相容性和生物可降解性,可通过自组装形成微球或纳米粒。近年来,Zein用作药物输送系统(drug delivery system,DDS)载体材料成为研究热点,可通过Zein的自组装特性将药物包载在其内部获得Zein-DDS微粒。本文简述了Zein的特性及其作为DDS载体的优势,总结了Zein-DDS的制备方法、性能及Zein的修饰改性研究进展,指出Zein具有较强的疏水性和抗胃酸分解特性,Zein-DDS可有效地提高药物稳定性、延缓药物释放及增强药物靶向性。因此,Zein用作DDS的载体具有广阔的应用前景,今后的研究工作将会在拓宽研究领域、改进制备方法及Zein的修饰改性等方面展开。     

VE-玉米醇溶蛋白纳米胶囊工艺及抗氧化性能

以维生素E为芯材、玉米醇溶蛋白（Zein）为壁材、醇醚糖苷（AEG）为乳化剂、甘油（VG）为助乳化剂,通过正负相凝聚法制备维生素E-玉米醇溶蛋白纳米胶囊（VE/Zein-NC）。以包封效率和粒径为指标,通过单因素试验优化制备工艺,并研究纳米胶囊抗氧化能力。结果表明,VE/Zein-NC的最佳制备工艺为：玉米醇溶蛋白质量浓度为2.5 mg/m L、乙醇质量分数为75%、正负相体积比为1∶2。此条件所制备的VE纳米胶囊的包封率达到85.37%±0.46%,VE/Zein-NC平均粒径为305.49 nm,多分散系数为0.038,分散性良好,水分含量低,储存30 d后抗氧化能力可达未经处理VE的2.47倍。     

复合皂与合成皂的性能、化学性质和配方(四)

正(上接2018年第2期第63页)15温和度评估方法研究人员开发了体外各种蛋白质变性实验来预测表面活性剂对眼睛和皮肤刺激性情况(Paye,1999)。玉米醇溶蛋白(Zein)测试是一种广泛应用的体内筛选方法,用于评价局部对表面活性剂的容忍度。玉米醇溶蛋白可从玉米和角蛋白中获取。该实验方法是由Gotte发明的(Gotte,1966),是基于玉米蛋白的溶解性,该蛋白一般不溶于水,除非蛋白变性。在常温下将该蛋白与表面活性剂溶液     

玉米醇溶蛋白膜研究进展

介绍了玉米醇溶蛋白(Zein)的组成及结构,总结了Zein膜的制备方法、性能及用途,详述了为满足Zein膜在各种用途中的性能而进行的各种修饰改性,如提高机械性能、增强亲/疏水性、增强隔氧阻水性、增大载药量和载药效率及增强药物控缓释等。今后的工作,将围绕新的制备和复合改性方法、拓展Zein膜的应用领域、复合改性机理的研究及改善Zein膜用于药物控缓释时初期的爆发性释放等方面展开。     

温和低刺激皂基洗面奶的配方设计

通过脂肪酸盐与月桂醇聚醚羧酸钠不同浓度比对玉米醇溶蛋白的刺激性表征实验,以及角蛋白的溶胀实验,确定皂基与月桂醇聚醚羧酸钠的添加比例,从而得到温和低刺激皂基洗面奶配方。结果表明,该配方洗后不干燥,泡沫丰富,耐热耐寒的稳定性好。     

玉米醇溶蛋白的电纺研究进展

综述了玉米醇溶蛋白(Zein)的组成、性能以及应用情况,详细介绍了Zein在静电纺丝方面的研究.目前的研究表明:电纺Zein纳米纤维具有优良的生物相溶性、可降解性.降解产物无毒副作用等优点,且原料丰富,生产工艺简单,必将在生物医学领域具有广泛的应用前景.但必须在提高纤维的力学性能,开展生物实验等方面深入研究.     

The combined effects of lysozyme and ascorbic acid on microstructure and properties of zein-based films

Edible zein-based films containing lysozyme(LY) and ascorbic acid(AA) were developed in the presence of polyethylene glycol 400(PEG 400), the combined effects of LY and AA on the microstructure, mechanical properties and release properties of developed zein films were investigated in detail. The results of microstructure characterization indicated that zein-based films became compact and smooth, and LY aggregates were well distributed in the zein matrix because of the simultaneous addition of LY and AA. The results of mechanical tests showed that because of the synergistic effects of LY and AA on zein film, elongation at break of zein-based film could be up to 138%, which was 34.5 times higher than that of zein control film. LY release tests showed that when the concentration of AA was less than 3.1 mg·cm~(-2), the release rate of LY significantly decreased by 33.7%, and the total release increased by 80.6%. While the release profiles of AA showed that the release rate and total release of AA from the films containing LY increased by approximately 68.9% and 61.7% than the films without LY. Good antioxidant and sustained antimicrobial activities were found for the developed zein films.     

用玉米醇溶蛋白测试表面活性剂的刺激性

表面活性剂的刺激性不同，使醇溶蛋白水解出的氨基酸数量有所差别。zein测试方法就是通过测定zein值，即表面活性剂溶液中有醇溶蛋白时测得的氮含量和无醇溶蛋白时测得的氮含量之差，判断表面活性剂刺激性的强弱。使用自制的玉米醇溶蛋白，利用zein测试法，对月桂醇硫酸酯盐、月桂醇聚氧乙烯醚硫酸酯盐和不同碳链的烷基硫酸镁等表面活性剂的刺激性进行了评价。其实验结果与文献完全吻合，表明zein测试法具有很好的重复性和可信度。     

Zein nanoparticle‐embedded electrospun PVA nanofibers as wound dressing for topical delivery of anti‐inflammatory diclofenac

Bioactive wound dressings from poly(vinyl alcohol) (PVA) and zein nanoparticles (NPs) loaded with diclofenac (DLF) were prepared successfully by the single jet electrospinning method. DLF‐loaded zein NPs with an average diameter of ～228 nm were prepared using anti‐solvent precipitation method. The formulation of zein:DLF 1:1 exhibited optimum encapsulation efficiency of 47.80%. The NPs were characterized by dynamic light scattering, zeta‐potential measurement, and differential scanning calorimetry. In vitro, drug release profiles of the DLF‐loaded zein NPs, and PVA–zein NPs were also studied within 120 h and showed the release efficiency of nearly 80% from zein NPs. A more controlled release of DLF was achieved by embedding the zein NPs in the PVA nanofibers. Fourier transform infrared spectroscopy was used to analyze possible interactions between different components of the fabricated dressings. The mechanical properties of the developed dressings were also evaluated using uniaxial tensile testing. Young's modulus (E) of the dressings decreased after inclusion of zein NPs within the PVA nanofibers. Moreover, fibroblast culturing experiments proved that the composite dressings supported better cell attachment and proliferation compared to PVA nanofibers, by exhibiting moderate hydrophilicity. The results suggested that the electrospun composite dressing of PVA nanofibers and zein NPs is a promising topical drug‐delivery system and have a great potential for wound healing application. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 46643.     

复方烟酸辛伐他汀缓释片的制备及体外释放度研究

目的：制备复方烟酸辛伐他汀缓释片并考查其体外释放度。方法：采用亲水凝胶骨架材料（HPMC）制备复方烟酸辛伐他汀缓释片;采用紫外分光光度法,以去离子纯化水900mL为溶剂,篮法测定本品释放度,并与参比制剂进行比较。结果：释放曲线经相似因子（f2）判断,与参比制剂相似。结论：本品处方工艺稳定,重现性好,体外累积释放度符合要求。     

The Preparation of Capsaicin-Chitosan Microspheres (CCMS) Enteric Coated Tablets

This study aimed to research the preparation and content determination of capsaicin-chitosan microspheres (CCMS) enteric coated tablets. The core tablets were prepared with the method of wet granulation. Nine formulae were designed to determine the optimal formula of the core tablet. Eudragit L100 was used to prepare the CCMS enteric-coated tablets. The effect of enteric coated formulation variables such as content of talc (10%, 25% and 40%), plasticisers (TEC and DBS), dosage of plasticiser (10%, 20% and 30%) and coating weight (2%, 3% and 5%) were evaluated for drug release characteristics. The in vitro release was studied using 0.1 N HCl and pH 6.8 phosphate buffer. Enteric coated tablets without ruptures or swelling behaviour over 2 h in 0.1 N HCl indicated that these tablets showed acid resistance. The accumulated release rate in phosphate buffer (pH 6.8) revealed that the prepared tablets were able to sustain drug release into the intestine and a first-order release was obtained for capsaicin. This research is the first report of the preparation and content determination of CCMS enteric coated tablets. The sustained release behavior of enteric coated formulations in pH 6.8 phosphate buffer demonstrated that it would be a potential drug delivery platform for sustained delivery of gastric irritant drugs.     

玉米醇溶蛋白/鱼腥草黄酮复合纳米颗粒的制备及释放特性

为提高鱼腥草黄酮(The flavonoids of Houttuynia cordata,HCF)的稳定性,本研究制备了玉米醇溶蛋白/鱼腥草黄酮复合纳米颗粒(Zein/HCF composite nanoparticles,NZH)并对其进行表征,探究zein、HCF不同质量比对NZH的影响。结果表明,HCF与zein之间存在氢键、疏水及静电相互作用;加入HCF后,NZH熔融温度增加,热稳定性提高。随HCF的增加NZH平均粒径、PDI先减小后增大,zeta电位绝对值先增大后减小。其中,NZH-3(zein:HCF为20:3)的平均粒径、PDI值最小、粒径分布峰最窄、zeta电位绝对值最大,微观结构呈均匀分散的圆球状。HCF的加入使复合纳米颗粒具有较高的DPPH、ABTS自由基清除能力。与游离HCF相比,NZH-3具有较好的热处理保护能力。在模拟消化过程中,NZH-3经胃液消化120 min后HCF释放率仅为22.31%,在肠液消化中出现突释现象,胃肠液消化240 min,HCF释放率达到75.66%。NZH-3在模拟胃肠液中的释放趋势均符合Ritger-Peppas模型,但释放机制不同,胃液中属非Fick扩散机制,肠液中属Fick扩散机制。     

超临界CO2抗溶剂法制备白藜芦醇-玉米蛋白纳米粒

以玉米蛋白为壁材,白藜芦醇为模型药物,采用超临界CO2抗溶剂法制备负载白藜芦醇的玉米蛋白纳米粒,考察了过程参数压力、温度以及芯材质量比对白藜芦醇在纳米粒中负载量的影响。用紫外光谱分析、扫描电镜、X射线衍射分析等方法对样品进行了表征,并且对释放度进行研究。结果表明,随着超临界CO2压力的增加,白藜芦醇的负载量降低;随着温度和芯材质量比的增加,白藜芦醇的负载量升高。玉米蛋白和白藜芦醇形成Matrix结构,纳米粒为均匀球形颗粒。释放度实验显示,纳米粒中白藜芦醇的释放速度比原料的释放速度慢,有缓释作用。     

Zein-based microcapsule for vanillin sustained release

Currently, microcapsules are attracting great attention and becoming more and more widely used in sustained release system. In this research, zein-based microcapsules were prepared by anti-solvent precipitation technology and spray drying. The properties of as-prepared microcapsules were optimized by adjusting the reaction conditions and parameters such as the mass ratio of zein to vanillin, the drying temperature and whether polyethylene glycol was added or not. Meanwhile, the morphologies, chemical structures, and application performances of microcapsules were characterized and determined by SEM, FT-IR, TG, and so on. The results showed that the morphology of as-obtained microcapsules was a regular spherical shape with no cracks when the mass ratio of zein to vanillin was 1:1, the inlet temperature was 120 C and the polyethylene glycol was introduced. Meanwhile, it was also proved that the polyethylene glycol has a positive effect on the encapsulation efficiency in microcapsules. Moreover, zein microcapsules can effectively improve the thermal stability and release behavior of vanillin, meanwhile the exist of polyethylene glycol can further improve the properties of zein microcapsules. This research provided guidelines for the study of microcapsules.     

Controlled release of aspirin from pH‐sensitive chitosan/poly(vinyl alcohol) hydrogel

The aim of this study was to develop a cheap, pH‐sensitive enteric coating of aspirin with biocompatible polymers. A novel approach was used to develop enteric coating from chitosan (CS) and poly(vinyl alcohol) (PVA). Solutions of CS and PVA (5 : 1 mol ratio) were mixed and selectively crosslinked with tetraethoxysilane. IR analysis confirmed the presence of the incorporated components and the existence of siloxane linkages between CS and PVA. The crosslinking percentage and thermal stability increased with increasing amount of crosslinker. The response of the developed coating in different media, such as water, pH (nonbuffer and buffer), and ionic media showed hydrogel properties. All hydrogels showed low swelling in acidic and basic pH media, whereas maximum swelling was exhibited at neutral pH. This pH sensitivity of the hydrogel has been exploited as enteric coating for commercial aspirin tablets. The dissolution test of enteric‐coated aspirin tablet in simulated gastric fluid (pH 1.2) showed 7.11% aspirin release over a period of 2 h, whereas a sustained release of remaining aspirin (83.25%) was observed in simulated intestinal fluid (pH 6.8). © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

Multifunctional and Efficient Air Filtration: A Natural Nanofilter Prepared with Zein and Polyvinyl Alcohol

Nanofibers prepared by electrospinning technology possess large specific surface area and adjustable fiber diameter. Zein is a hydrophobic plant protein that can interact with pollutants in the air. In this work, using polyvinyl alcohol (PVA) as the auxiliary spinning polymer, zein based nanofiber materials are prepared for air filtration by varying zein/PVA ratio as well as the alcoholysis degree. With the increase of zein content, the diameter of nanofibers decreases gradually, and the nanofibers prepared by PVA with low alcoholysis degree exhibit better micromorphology. The zein based nanofibers prepared after denaturation of zein protein can interact with different types of pollutants. In addition, for the nanofibers prepared by PVA with different alcoholysis degree, the filtration efficiency increases with the increase of zein content when absorbing air pollution particles with different particle sizes and the alcoholysis degree affects the air filtration efficiency. Thus, through controlling zein content and alcoholysis degree, the filtration efficiency of nanofibers is promoted availably. These results indicate that the natural nanofilter prepared with zein and PVA can be used a multifunctional and efficient material for air filtration.