Oil Content and Fatty Acid Composition in Seeds of Three Safflower Species

Seeds of six safflower (C. tinctorius L.) genotypes and 19 accessions of two wild species were analyzed for oil and fatty acid composition. Oil content ranged from 29.20 to 34.00, 20.04 to 30.80 and 15.30 to 20.80% in C. tinctorius, C. oxyacantha Bieb. and C. lanatus L., respectively. The main fatty acids of oleic, linoleic, palmitic and stearic acids composed 96–99% of the total fatty acids in all species. The sum of myristic, palmitoleic, arachidic, and behenic fatty acids in oil of the species ranged from 0.43 to 0.57%. The oleic acid in seed oil of C. tinctorius, C. oxyacantha and C. lanatus ranged from 12.24 to 15.43, 14.11 to 19.28 and 16.70 to 19.77%, respectively. The corresponding ranges for linoleic acid were 71.05 to 76.12, 63.90 to 75.43 and 62.47 to 71.08%. Palmitic acid in seed oil varied from 5.48 to 7.59% in C. tinctorius, 6.09 to 8.33% in C. oxyacantha and 7.44 to 8.78% in C. lanatus. The stearic acid of the seed oil showed a variation of 1.72 to 2.86, 2.50 to 4.87 and 3.14 to 4.79% in genotypes of these species, respectively. The fatty acids composition of oil among the cultivated and wild species were not considerably different, indicating that seed oil of the wild safflower is possibly suitable for human consumption and industrial purposes.     

Supercritical Carbon Dioxide Extraction,Fatty Acid Composition,Oxidative Stability,and Antioxidant Effect of Torreya grandis Seed Oil

Torreya grandis seed oil (TGSO) extraction with supercritical carbon dioxide was explored from the extraction conditions, fatty acid composition, its oxidative stability and antioxidant activity in a bench‐scale apparatus. An L₉(3⁴) orthogonal design was applied to optimize extraction parameters. The results demonstrated that the maximum yield of 94.57 % was obtained at 45 MPa, 4 h and 50 °C. There were 18 kinds of compounds found within TGSO; the predominant ingredient was linoleic acid (42.02 %), followed by oleic acid (32.14 %) and dihomo‐γ‐linolenic acid (9.80 %). The IC₅₀ values for 1,6‐bis(diphenylphosphino) hexane radical (DPPH), hydroxyl radical (HO?) and superoxide radical (O₂^·?) were 5.61, 3.16 and 4.20 mg/mL, respectively.     

Fatty Acid Profile of Kenaf Seed Oil

The fatty acid profile of kenaf (Hibiscus cannabinus L.) seed oil has been the subject of several previous reports in the literature. These reports vary considerably regarding the presence and amounts of specific fatty acids, notably (12,13-epoxy-9(Z)-octadecenoic (epoxyoleic) acid, but also cyclic (cyclopropene and cyclopropane) fatty acids. To clarify this matter, two kenaf seed oils (from the Cubano and Dowling varieties of kenaf) were investigated regarding their fatty acid profiles. Both contain epoxyoleic acid, the Cubano sample around 2 % and the Dowling sample 5-6 % depending on processing. The cyclic fatty acids malvalic and dihydrosterculic were identified in amounts around 1 %. Trace amounts of sterculic acid were observed as were minor amounts of C17:1 fatty acids. The results are discussed in the context of the fatty acid profiles of other hibiscus seed oils.     

环氧油脂肪酸组成分析研究

通过薄层色谱、气相色谱、色质联用等技术,首次得到了油脂环氧化反应期间的脂肪酸环氧化反应规律:开始反应阶段,高含量不饱和脂肪酸反应速率高于低含量不饱和脂肪酸;环氧化反应期间,多不饱和脂肪酸首先生成单环氧酸,之后再逐渐生成二环氧酸,最后生成三环氧酸;富含亚麻酸的油脂环氧化反应时有更易于开环反应的趋向,其次是富含亚油酸的油脂,再次是富含油酸的油脂.实验结果表明,不同环氧油原料在进行环氧化反应时需要控制不同的反应条件,以避免开环副产物量的增加,从而制备得优质环氧油产品.     

Physicochemical Properties and Fatty Acid Profile of Phoenix Tree Seed and its Oil

Various components of Phoenix tree (Firmiana simplex) seed were determined. Oil, protein, moisture, ash, and fiber accounted for 27.8 ± 0.3, 19.7 ± 0.4, 7.5 ± 0.2, 4.4 ± 0.3, and 31.23 ± 0.93 % (w/w) of the seed, respectively. The acid value, peroxide value, saponification value, and unsaponifiable matter content of Phoenix tree seed oil extracted using the Soxhlet method were 3.73 ± 0.02 mg KOH/g, 1.97 ± 0.21 mmol/kg, 183.74 ± 2.37 mg KOH/g, and 0.90 ± 0.05 g/100 g, respectively. The total tocopherol content was 54.5 ± 0.5 mg/100 g oil, which consisted mainly of δ‐tocopherol (29.5 ± 0.6 mg/100 g oil) and γ‐tocopherol (13.8 ± 0.8 mg/100 g oil). Linoleic acid (L, 30.2 %), oleic acid (O, 22.2 %), and sterculic acid (S, 23.2 %) were the main unsaturated fatty acids of Phoenix tree seed oil. The saturated fatty acids included palmitic acid (17.4 %) and stearic acid (St, 2.9 %). The work shows the first report of sterculic acid in seeds of this species. This oil can be used as a raw material to produce sterculic acid.     

Comparative Effects of Sandalwood Seed Oil on Fatty Acid Profiles and Inflammatory Factors in Rats

The aim of the present study was to investigate the effect of sandalwood seed oil on fatty acid (FA) profiles and inflammatory factors in rats. Fifty male Sprague–Dawley rats were randomly divided into five different dietary groups: 10 % soybean oil (SO), 10 % olive oil (OO), 10 % safflower oil (SFO), 10 % linseed oil (LSO) and 8 % sandalwood seed oil blended with 2 % SO (SWSO) for 8 weeks. The SWSO group had a higher total n-3 polyunsaturated fatty acids (PUFA) levels but lower n-6:n-3 PUFA ratios in both adipose tissue and liver than those in the SO, OO and SFO groups (p < 0.05). Although the SWSO group had a much lower 18:3n-3 level (4.51 %) in their dietary lipids than the LSO group (58.88 %), the levels of docosahexaenoic acid (DHA: 22:6n-3) in liver lipids and phospholipids of the SWSO group (7.52 and 11.77 %) were comparable to those of the LSO group (7.07 and 13.16 %). Ximenynic acid, a predominant acetylenic FA in sandalwood seed oil, was found to be highly incorporated into adipose tissue (13.73 %), but relatively lower in liver (0.51 %) in the SWSO group. The levels of prostaglandin F_2α, prostaglandin E₂, thromboxane B₂, leukotriene B₄, tumor necrosis factor-α and interleukin-1β in both liver and plasma were positively correlated with the n-6:n-3 ratios, suggesting that increased n-6 PUFA appear to increase the formation of pro-inflammatory cytokines, whereas n-3 PUFA exhibit anti-inflammatory activity. The present results suggest that sandalwood seed oil could increase tissue levels of n-3 PUFA, DHA and reduce the n-6:n-3 ratio, and may increase the anti-inflammatory activity in rats.     

Seasonal Variation of the Chemical Content and Fatty Acid Composition of Mantle and Tentacle of Male and Female Sepia officinalis

The goal of this study was to evaluate the chemical composition and the fatty acid profile of mantle and tentacle of male and female Sepia officinalis, sampled at four seasons from the Mediterranean sea of Tunisia. S. officinalis were found to be rich in glycogen, protein and oil, and significant differences were observed between samples. The level of saturated fatty acid and unsaturated fatty acid showed significant variability among sex and during seasons. DHA and EPA, as polyunsaturated fatty acids, were the most abundant in all samples (14.8–27.8 % and 10.4–18.3 %, respectively). Oleic acid was the most abundant monounsaturated fatty acids (1.63–4.52 %). Σn3 and Σn6 was remarkably different between seasons and among sex. This study could be suitable for the development of reliable guide of fatty acid accumulation in cephalopod.     

气相色谱-质谱联用分析美藤果油脂肪酸组成

采用超临界CO2流体萃取法提取美藤果种子中的脂肪油,甲酯化后运用GC-MS联用技术对其中的脂肪酸组成进行分析。结果表明:采用超临界CO2萃取法提取美藤果种子脂肪油得率为48.75%,所得脂肪油中含有11种脂肪酸,主要为亚麻酸(46.38%)、亚油酸(34.19%)、棕榈酸(10.89%)和硬脂酸(7.60%),其中不饱和脂肪酸含量高达81.18%以上,表明美藤果种子富含不饱和脂肪酸,具有较高的营养价值和保健功能。     

The Fatty Acid Profile and Phenolic Composition of Descurainia sophia Seeds Extracted by Supercritical CO2

Oil and phenolics were extracted from Descurainia sophia (Sophia) seeds by a supercritical CO₂ system. Extractions were conducted in two sequential steps, first using 100 % CO₂ and then adding 10 % ethanol as co‐solvent. The extracts were collected in each step using two separate collectors operating at different pressures. The extraction run was 3 and 4 h for the first period, and 2 h for the second period. The majority of the oil was collected in the first extraction period while phenolic compounds were obtained in the second extraction period. A combined mode of static/dynamic extraction (3 h running and 1 h soaking in CO₂) was also used in the first extraction period, which enhanced the total extraction yield (29.3 ± 0.5 %) and was comparable to the 4 h extraction yield (31.4 ± 0.1 %). The total fatty acid (FA) content of oil in collector 1 (0.94 g) was nearly twice that in collector 2 (0.60 g). The oil contained 14 FAs with α‐linolenic being predominant (48.5 %), with a total 91.1 % unsaturated FAs, a ω3/ω6 ratio of 2.7, and an erucic acid content of 6.2 %. More than 10 phenolic compounds were detected by HPLC in the Sophia seed extracts of which sinapic acid was the dominant compound. Sophia seed extracts showed high levels of antioxidant activity. These results suggest that Sophia seed oil and phenolics have the potential for functional food and pharmaceutical applications.     

Chemical Properties and Fatty Acid Composition of Thunbergia fragrans Roxb. Seed Oil

The physicochemical and fatty acid compositions of seed oil extracted from Thunbergia fragrans were determined. The oil content, free fatty acids, peroxide value, saponification value and iodine value were 21.70 %, 2.25 % (as oleic acid), 9.6 (mequiv. O₂/kg), 191.71 (mg KOH/g) and 127.84 (g/100 g oil) respectively. The fatty acid profiles of the methyl esters showed the presence of 90.16 % unsaturated fatty acids and 9.84 % saturated fatty acids. Palmitoleic acid, which is usually found in marine foods and is unique in seed oils of botanical origin, was the major component (79.24 %). The oil can also be used in industries for the preparation of liquid soaps, shampoos and alkyd resin.     

Fatty Acid and Phytochemical Compositions of Plantago Seed Oils and Their Functionalities

To develop the potential applications of Plantago plants, seed oils were extracted from 14 cultivars of Plantago around China. Their fatty acid profiles, tocopherols, carotenoid compositions, anti‐inflammatory and antioxidant properties were also investigated. The Plantago seed oils (PSO) were abundant in linolenic acid from 11.12 to 29.36 g/100 g oil and had low fatty acid ratio of n‐6/n‐3 ratio matched with the dietary recommendations. The tocopherol contents of PSO ranged from 693.25 to 3708.80 μg/g and the lutein contents ranged from 2.29 to 26.68 μg/g. The PSO showed significant inhibitory effects on TNF‐α, IL‐1β, and COX‐2 mRNA expression in RAW 264.7 mouse macrophage cells induced by LPS. In addition, the properties on scavenging DPPH, oxygen and hydroxyl radicals indicated that PSO had potential antioxidant properties. The results could develop PSO as novel functional foods to improve human health.     

Fatty Acid Composition of Irvingia gabonensis and Treculia africana Seed Lipids and Phospholipids

Lipid compositions of two non-conventional oilseeds (Irvingia gabonensis and Treculia africana) were studied. Total lipids were extracted by the Folch method and phospholipids were isolated by solid phase extraction. Fatty acid compositions of total lipids and phospholipids were determined by gas chromatography. Phospholipid classes and tocopherols were quantified by HPLC. The major fatty acids in I. gabonensis seed total lipids were myristic (41.4–48.9 %) and lauric (39.8–46.8 %) while those in T. africana seed were linoleic (29.1–31.4 %) and oleic (22.9–25.9 %). The principal fatty acid at the sn-2 position of I. gabonensis seed triacylglycerols was myristic (49.5 %) while that of T. africana was linoleic (50.6 %). Phospholipid content of crude T. africana seed oil was 3.3 % and that of I. gabonensis was 0.1–0.3 %. The composition and distribution of saturated fatty acids in I. gabonensis seed lipids suggest that it may contribute to cardiovascular disease risk factors among the Igbo people in Nigeria that use the seed as food ingredient.     

Influence of Fatty Acid Profile of Total Parenteral Nutrition Emulsions on the Fatty Acid Composition of Different Tissues of Piglets

Total parenteral nutrition (TPN) studies in human babies of very-low-birth-weight suggest that the lipid emulsions currently available are not optimum for neonatal nutrition. Since fatty acid metabolism in human and pigs is very similar, the present study examines how lipid emulsions used in clinical TPN (i.e. ClinOleic, Intralipid, Lipofundin or Omegaven), with different fatty acid compositions, administered to neonatal piglets for 7 days, influenced their tissue fatty acid composition as compared to those enterally fed with a sow milk replacer. A positive linear relationship was found between the proportion of all individual fatty acids in the lipid emulsions or in the milk replacer versus those in plasma, skeletal muscle, subcutaneous fat, liver, heart, pancreas, stomach or intestine total lipids or in brain phospholipids, the latter showing the lowest correlation coefficient. With the exception of brain, the proportion of either oleic acid or alpha-linolenic acid in the individual tissues was correlated with those present in the corresponding lipid emulsion or milk replacer, whereas the proportion of linoleic acid correlated significantly with all the tissues studied. With the exception of brain phospholipids, both eicosapentaenoic and docosahexaenoic acids were higher in the tissues of piglets receiving Omegaven than in all other groups. In conclusion, with the exception of the brain, fatty acid composition of plasma and different tissues in piglets are strongly influenced by the fatty acid profile of TPN emulsions. Fatty acid composition of brain phospholipids are, however, much less influenced by dietary composition, indicating an active and efficient metabolism that ensures its appropriate composition at this key stage of development.     

The Interaction of the Soybean Seed High Oleic Acid Oil Trait With Other Fatty Acid Modifications

Oil value is determined by the functional qualities imparted from the fatty acid profile. Soybean oil historically had excellent use in foods and industry; the need to increase the stability of the oil without negative health consequences has led to a decline in soybean oil use. One solution to make the oil stable is to have high oleic acid (>70%) and lower linolenic acid content in the oil. Other fatty acid profile changes are intended to target market needs: low‐saturated fatty acid and high stearic acid content in the oil. The objective of this study is to determine the interaction of the high oleic acid oil trait with other alleles controlling fatty acid profiles. Soybean lines containing high oleic acid allele combinations plus other fatty acid modifying alleles were produced, and the seed was produced in multiple field environments over 2 years. Stable high oleic acid with low linolenic acid (<3.0%) was achieved with a 4‐allele combination. The target of >20% stearic acid in the seed oil was not achieved. Reducing total saturated fatty acids below 7% in a high oleic acid background was possible with mutant alleles of both an acyl‐ACP thioesterase B and a β‐ketoacyl‐[acyl‐carrier‐protein] synthase III gene. The results identified allele combinations that met the target fatty acid profile thresholds and were most stable across environments.     

Oil Content and Composition of Sesame (Sesamum indicum L.) Genotypes as Affected by Irrigation Regimes

The fatty acid composition of sesame seed oil determines its commercial value, and drought stress and genotype may affect both the quality and quantity of oil that is extractable by sesame seed processors. Therefore, this experiment was conducted in a field located in Isfahan, Iran, to determine the effect of three irrigation regimes [55 % (I ₁, as control), 75 % (I ₂) and 85 % (I ₃) of depletion of soil available water] on the oil content and composition of ten sesame genotypes (Isfahan4, Shahreza, Borazjan, Ahvaz, Kal, Shiraz, Markazi, Ardestan, Ultan and Isfahan1). Seed oil content and palmitic, linoleic, linolenic, stearic and oleic acid contents were determined during 2010–2012. Shahreza under I ₁, Shahreza and Ardestan under I ₂ and Isfanahan4 under I ₃ irrigation regimes produced the highest oil content, while Kal under I ₁ and I ₂ and Isfahan4 under I ₃ irrigation regimes produced the highest oil yield. Ultan had the lowest palmitic acid content under all three irrigation regimes, while Isfahan4 had the highest reduction in palmitic acid content under I ₂ and I ₃. Borazjan produced the lowest stearic and highest linoleic acid contents under the I ₁, I ₂ and I ₃ irrigation regimes. Kal, Kal and Ardestan, and Ardestan and Isfahan1 produced the highest oleic acid under I ₁, I ₂ and I ₃, respectively. Shahreza under I ₁ and I ₂ and Isfahan4 under I ₃ had the highest linolenic acid content. The results showed that the sesame oil content and composition were genotype-drought level specific, and based on oil yield production and percent reduction in saturated and increase in unsaturated fatty acid contents, Isfahan4 was the most drought-tolerant genotype.     

Fatty Acid Composition of the Oil from Developing Seeds of Different Varieties of Safflower (Carthamus tinctorius L.)

Fatty acid composition and moisture and oil content were determined for Montola-2001 and Centennial safflower varieties at three different harvest dates from flowering to maturity, which were grown as autumn and spring crops in two different locations in 2001–2002 and 2002–2003. The experiment was carried out using split–split plots in a randomized complete block design with three replicates. Sowing dates affected oil content and fatty acid compositions significantly (P < 0.01), whereas moisture content in both years was not significantly affected. Moisture content declined 15 days from flowering period to maturity, while oil content increased. The rate of the palmitic acid formation decreased in both varieties 15 days from flowering period to maturity, whereas formation rates of the oleic and linoleic acids increased in Montola-2001 and Centennial varieties, respectively.     

棉籽酸化油的理化性质及脂肪酸组成分析

采用气相色谱法分析了棉籽酸化油的脂肪酸组成,并对其理化性质进行了研究。分析结果表明,棉籽酸化油的含油率为91.33%,酸值为144.35mgKOH/g,碘值为116.58gI2/100g,皂化值为199.80mgKOH/g;其主要脂肪酸为棕榈酸(21.29%)、硬脂酸(2.29%)、油酸(23.72%)、亚油酸(50.23%)和亚麻酸(0.39%),其中不饱和脂肪酸的含量高达74%,具有很高的工业利用价值。     

Fatty Acid Composition, Antioxidant Properties, and Antiproliferative Capacity of Selected Cold-Pressed Seed Flours

Cold-pressed seed flours from pumpkin, parsley, mullein, cardamom, and milk thistle were examined for total oil, fatty acid profile of the oil, total phenolic content (TPC), scavenging activities against peroxyl (ORAC), hydroxyl (HOSC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (RDSC) radicals, and antiproliferative capacity against HT-29 human colon cancer cells. The cold-pressed parsley seed flour contained a very high concentration of total oil—17.6 g/100 g flour—with primarily C18:1 fatty acid at 86.2 g/100 g fatty acids. All other flour oils had relatively high levels of saturated fats, ranging from 39.0 to 62.9 g/100 g fatty acids. The tested seed flours demonstrated significant TPC and free radical scavenging activities. Milk thistle seed flour had the highest TPC value of 25.2 mg gallic acid equivalent per g flour (GAE mg/g) followed by that of parsley seed flour at 8.1 GAE mg/g. Milk thistle seed-flour extract also had significantly higher antioxidant activities than all other extracts against all tested radicals. The milk thistle seed-flour extract had an ORAC value of 1131 μmol trolox equivalents (TE) per g flour (TE μmol/g), a HOSC value of 893 TE μmol/g, and an RDSC value of 61 TE μmol/g. Also, ORAC, HOSC, and TPC values were significantly correlated (P < 0.01) under the experimental conditions. The cold-pressed milk thistle seed flour inhibited the proliferation of HT-29 cancer cells in a dose-dependent manner. Results from this study suggest that these cold-pressed seed flours may serve as natural sources of antioxidants and may be used to improve human health.     

Seed Oil Content and Fatty Acid Composition from Different Populations of Calotropis procera (Aiton) W. T. Aiton (Apocynaceae)

Calotropis procera is a soft-wooded, evergreen, perennial shrub species which grows in a variety of environments including dry habitats such as the Brazilian semi-arid region. This study aimed to evaluate the variation in the oil content and fatty acid composition of C. procera seeds from four climatically different locations in the state of Pernambuco in the northeast of Brazil. The seed oil content of C. procera ranged from 19.7 to 24.0 %. Five main fatty acids were identified, with a predominance of unsaturated linoleic and oleic acids (approximately 70 %). Our results suggesting that environmental conditions influenced the seed oil biosynthesis of C. procera, because the localities with high temperature and low precipitation had increased oil content and total biosynthesis of saturated fatty acids. Multivariate analysis showed high similarity among three localities which share certain physiographic characteristics and climatic conditions such as temperature and precipitation values. The oil profile of C. procera presents interesting features that highlight its potential as a future alternative for the biodiesel market, especially in semi-arid regions.