Dynamic Effects of Cerium on Syntheses of Soluble Protein and Taxol in Suspension Culture of Taxus Chinensis Var. Mairei Cells

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Ce4+对东北红豆杉细胞有丝分裂的影响

以悬浮培养的东北红豆杉细胞为材料,研究了不同浓度的Ce4 在不同处理时间对细胞有丝分裂的影响。结果显示,Ce4 能诱发东北红豆杉细胞多种异常有丝分裂,在浓度为0.5mmol/L、处理时间为5h时,即开始出现异常有丝分裂现象,而且细胞有丝分裂指数(MI)比对照组明显降低(P<0.05),表明细胞有丝分裂受到抑制,Ce4 对细胞有丝分裂过程有显著影响。Ce4 具有一定遗传毒性。     

Effect of La, Ce on Taxus Cuspidata Cell Growth, Biosynthesis and Release of Taxol

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Hydrolysis Activities of Resins of Complexes Made from Polysaccharides and Ce4+

Resins of complexes made from agarose-Ce4 (RCA-Ce) and chitosan-Ce4 (RCC-Ce) were prepared. Hydrolysis rates of methamidophos, omethoate and chlorpyrifos treated by RCA-Ce were 32.39%, 27.12% and 46.62%, respectively, those of chlorpyrifos and methamidophos in mung bean sprout juice 38.28% and 35.45%, and that of chlorpyrifos in tea extract 59.76%. Hydrolysis rates of protein in tea extract treated by RCA-Ce increased by 86.46%. RCC-Ce could be employed in the apple juice production. The contents of methamidophos and parathion-methyl in the juice treated by RCC-Ce decreased 58.76% and 71.92%. Furthermore, RCC-Ce was used to clarification for beer by the ways of a column. RCC-Ce could hydrolyze sensitive protein and increase contents of free amino acid in the beer. Therefore, the beer treated by RCC-Ce could stay oxidation and aging procedure due to some metal ions being absorbed by RCC-Ce.     

Preparation of Cordierite Ceramic Using Mixtures of Ce4+-Modified Amorphous Powder and Oxide Powders

The cordierite ceramic with a high strength and a low thermal expansion coefficient was prepared by using mixtures of Ce4 -modified cordierite amorphous powder and oxide powders of MgO, Al2O3 and SiO2 as starting materials. The transformation procedure of the powders was analyzed by XRD method and the surface morphology of ceramics was examined by SEM method. The flexural strength was measured in a mechanical property testing machine using the Three-Point-Bending sample. Experimental results show that the ceramic added with 10%(mass fraction) of Ce4 -modified amorphous powder is composed of α-cordierite and small amount of CeO2 particles, and has a porosity of 15%(volume fraction) and a flexural strength of 32.6 MPa when sintered at 1350 ℃ for 3 h. It is suggested that this low-cost process is suitable for preparing the components such as supports of vehicle exhaust converters and diesel particulate trappers.     

稀土元素铈对红豆杉细胞膜透性的影响

用EvansBlue染色、细胞培养液电导率测定、MDA含量检测、电镜技术研究Ce4 对悬浮培养的东北红豆杉细胞膜的影响。结果表明:1mmol/LCe4 诱导后第5天,EvansBlue染色结果、细胞培养液的电导率、MDA含量显著地增加,细胞膜透性明显增大,细胞膜的完整性也受到一定程度的破坏,透射电镜观察细胞超微结构发生变化,说明1mmol/LCe4 能导致悬浮培养的红豆杉细胞膜的损伤。     

硝酸铈对红豆杉细胞培养及紫杉醇合成的影响

本文采用电子显微镜及聚丙烯酰胺凝胶电泳等方法 ,研究了稀土对红豆杉细胞培养中生物量积累及紫杉醇合成与释放的影响 ,同时进一步探讨了稀土调控次生代谢的机制。结果表明 ,适量稀土可促进紫杉醇合成 ,并对细胞的酶系统及超微结构产生一定影响。     

Adsorption of Ce(Ⅳ) Anionic Nitrato Complexes onto Anion Exchangers and Its Application for Ce(Ⅳ) Separation from Rare Earths(Ⅲ)

Ce(Ⅳ) nitrato complexes were adsorbed on two anion exchangers based on polyvinyl pyridine (PVP) and quaternized PVP incorporated into porous silica matrix. The effect of nitric acid concentration (0.5～6 mol·L-1) and temperature (278～318 K) on Ce(Ⅳ) sorption efficiency was investigated. Sorption increased with increasing nitric acid concentration, indicating that [Ce(NO3)6]2- complex is the main adsorbed Ce(Ⅳ) species. Oxidation of sorbents by adsorbed Ce(Ⅳ) species resulting in Ce(Ⅲ) release to the solution was observed. Pyridine based anion exchangers exhibited higher oxidation stability compared to the commercial strong base anion exchanger. Ce(Ⅳ) reduction was temperature dependent and obeyed pseudo-first-order reaction kinetics. Column separation of Ce(Ⅳ) from La(Ⅲ) and Y(Ⅲ) was carried out from 6 mol·L-1 nitric acid with PVP based anion exchanger. Reasonable Ce(Ⅳ) breakthrough capacity (0.7 mol·kg-1 PVP) was achieved. No remarkable decrease of capacity was observed within 3 consequent runs. In contrast, Ce(Ⅲ) leakage due to reduction decreased and breakthrough capacity slightly increased. This effect was more pronounced with increasing temperature. Regeneration with 0.1 mol·L-1 nitric acid was successful (recovery 100%±4%) and Ce solution of high purity (＞99.97%) with respect to La and Y content was gained.     

Adsorption of Ce（ Ⅳ ） Anionic Nitrato Complexes onto Anion Exchangers and Its Application for Ce （Ⅳ） Separation from Rare Earths （ Ⅲ ）

Ceriumis one of the cheapest[1]and most abun-dant rare earths (RE) .However ,high purityis usual-ly required for its utilization in industry , where it isusedfor sulfur control insteels ,pyrophoric alloys ,ce-ramic ,catalyst support ,polishing powders ,etc .In its minerals ,as well as in the spent nuclearfuel ,ceriumis accompanied by other RE.They basi-cally exist in solution as stable RE(Ⅲ) species ,which makes their mutual separation rather difficult .In contrast to other RE, Ce(Ⅲ) can…     

Effect of Cerium on Mechanical Properties and Morphology of ZZn4-1 Alloy

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