Subunit composition of delta-crystallin from embryonic chick lens. Analysis of methionine-containing tryptic peptides and cyanogen bromide peptides

The predominant protein in the embryonic chick lens, delta-crystallin, is composed of four subunits with molecular weights near 50,000. The degree to which these 4 polypeptides are the same or dissimilar was explored in delta crystallin purified from 15-day-old embryonic chick lenses by relating the numbers of methionine-containing tryptic peptides and cyanogen bromide (CNBr) peptides derived from the native protein to the average number of methionine residues per subunit. Amino acid analyses indicated that 1 mol of native delta-crystallin contains approximately 32 methionine residues, leading to an average of 8 methionine residues per subunit. Approximately equal amounts of 8 methionine-containing tryptic peptides were resolved by two-dimensional thin layer separation on cellulose sheets and by isoelectric focusing in polyacrylamide gels. Nine CNBr peptides were resolved by a combination of electrophoresis in sodium dodecyl sulfate (SDS)-polyacrylamide gels and chromatography on SDS-hydroxylapatite columns. The additive molecular weight of the 9 CNBr peptides was very close to the delta-crystallin subunit molecular weight of 50,000. Thus, the subunits of 15-day-old embryonic chick delta-crystallin have similar sequence of encoded amino acids.     

The separation of ipratropium bromide and its related compounds

Ipratropium bromide, the active component in ipratropium bromide metered dose inhalers (MDI), is used as a bronchodilator for the maintenance and treatment of bronchospasms associated with chronic obstructive pulmonary disease (COPD). The separation of ipratropium bromide, tropic acid, N-isopropyl-nor-atropine (NINA), 8-s ipratropium bromide, apo-ipratropium bromide and the excipients found in the formulation is important for analyzing raw materials and stability samples. We now report a reversed-phase HPLC method that can be used for separating ipratropium bromide and its related compounds, using an acetonitrile/potassium phosphate buffer (100 mM, pH 4.0) gradient mobile phase. Previous methods used for separating ipratropium bromide from its related compounds involved reversed-phase ion-pairing HPLC with UV detection. These methods exhibited less reproducibility, less ruggedness and required a high flow rate. The reported method is linear from 10 to 1000 micrograms ml-1 with a limit of detection of 60 ng ml-1. In addition, analysis of samples subjected to accelerated stability conditions showed that all degradants are resolved from the active component, resulting in stability-indicating assay. This assay also saved mobile phase and eliminated problems associated with ion-pairing reagents.     

游离氰滴定法的铜锌干扰

游离氰滴定法测氰化液中游离氰化钠时铜锌络离子的部分解离造成显著的正偏差，根据溶液理论对锌的干扰进行了分析和计算，并实际测定了氰化液中铜锌络离子的平均配位数变化情况，给出了铜辞干扰量的计算公式，探讨了铜锌干扰的实际校正方法．     

氨溴素联合地塞米松治疗急性喘息性支气管炎疗效观察

目的:观察地塞米松联合氨溴素(沐舒坦)注射液治疗小儿急性喘息性支气管炎临床疗效.方法:96例喘息性支气管炎患儿随机分为两组,对照组30例,治疗组66例,对照组采用常规治疗的基础上用庆大霉素联合地塞米松雾化吸入治疗,观察组在常规治疗的基础上用沐舒坦注射液15mg联合地塞米松5mg加入生理盐水20ml雾化吸入.结果:治疗组在短时间内改善肺部罗音的效果,总有效率及治愈明显优于对照组,二者比较差异有统计意义(p<0.05).结论:采用氨溴素联合地米松塞治疗小儿喘息性支气管炎可缩短住院病程,疗效满意,值得推广.     

电解法处理含氰镀铜废液的试验研究

讨论了电解法处理含氰废水的原理,利用实际含氰镀铜废液和自行设计的新型电解槽,探讨了各种因素对脱氰效果的影响。在实验的基础上用正交实验法确定了电解的最佳条件为:t=12min,I=2.5A,废水中加盐量18g/l,絮凝剂添加量100mg/l,废水从原来含氰化物25.mg/l,含铜量35.mg/l,降为含氰0.14mg/l,含铜0.42mg/l,去除率分别为99.44%和98.8%,出水达到排放标准。     

焦化酚氰废水在炼铁烧结系统的综合利用

介绍安钢焦化酚氰废水的基本情况和处理现状;针对酚氰废水存在的问题,通过一系列的技术改造,实现了焦化酚氰废水在炼铁烧结系统的综合利用,解决了困扰钢铁行业的环保难题,顺利完成工业废水零排放的目标。     

黄金氰化生产中含氰污水处理与零排放的实现

阐述了我国黄金氰化生产中含氰污水处理的方法与进展，以及实现含氰污水零排放应具备的条件与方法。     

山西焦化厂酚氰废水处理现状及发展趋势展望

简要介绍山西焦化厂酚氰废水处理工艺及回用现状,并对干熄焦技术实施以后所带来的酚氰废水出路问题进行了探讨,对焦化酚氰废水的深度处理工艺和回用途径进行了分析,提出了减量化和资源化是焦化企业酚氰废水"零排放"的根本出路。     

The Stimulated Raman Spectrum of Symmetric 13C Cyanogen, 13C2N2

BACKGROUND: The authors examined the interaction of ketamine with recombinant mu, kappa, and delta opioid receptors and recombinant orphan opioid receptors expressed in Chinese hamster ovary cells (CHO-mu, CHO-kappa, CHO-delta, and CHO(ORL1), respectively). METHODS: CHO-mu, CHO-kappa, and CHO-delta membranes were incubated with the opioid receptor radioligand [3H]diprenorphine at room temperature. Ketamine (racemic, R(-) and S(+)) was included at concentrations covering the clinical range. CHO(ORL1) membranes were incubated with [125I]Tyr(14)nociceptin and racemic ketamine at room temperature. The effects of racemic ketamine and selective opioid receptor agonists (mu: [D-Ala2, MePhe4, Gly(ol)5] enkephalin (DAMGO); kappa: spiradoline or delta: [D-pen2, D-pen5] enkephalin (DPDPE)) on forskolin-stimulated cyclic adenosine monophosphate formation also were examined. Data are mean +/- SEM. RESULTS: Racemic ketamine increased the radioligand equilibrium dissociation constant for [3H]diprenorphine from 85+/-5 to 273+/-11, 91+/-6 to 154+/-16, and 372+/-15 to 855+/-42 pM in CHO-mu, CHO-kappa, and CHO-delta, respectively. The concentration of radioligand bound at saturation was unaffected. In CHO-mu and CHO-kappa cells, racemic ketamine did not slow the rate of naloxone-induced [3H]diprenorphine dissociation. Ketamine and its isomers also displaced [3H]diprenorphine binding to mu, kappa, and delta receptors in a dose-dependent manner, with pKi values for racemic ketamine of 4.38+/-0.02, 4.55+/-0.04, and 3.57+/-0.02, respectively. S(+)-ketamine was two to three times more potent than R(-)-ketamine at mu and kappa receptors. Racemic ketamine displaced [125I]Tyr(14)nociceptin with an estimated affinity constant of 0.5 mM. Racemic ketamine inhibited the formation of cyclic adenosine monophosphate (naloxone insensitive) in a dose-dependent manner (concentration producing 50% inhibition approximately 2 mM) in all cell lines, including untransfected CHO cells. Ketamine (100 microM) reversed DAMGO (mu) and spiradoline (kappa) inhibition of formation of cyclic adenosine monophosphate. CONCLUSIONS: Ketamine interacts stereoselectively with recombinant mu and kappa opioid receptors.     

焦化酚氰废水工艺改进及综合利用

针对朝阳钢铁焦化酚氰废水工艺存在的问题,通过应用斜孔塔盘、可提升式曝气器和改造臭氧催化氧化系统等措施,使酚氰废水出水达标排放;并将生产排污水及富余脱硫液回用于酚氰废水,实现了废水的综合利用。     

焦化厂酚氰污水处理新工艺及其应用

由于焦化厂酚氰污水中CODcr、焦油类、酚氰、NH3-N等污染物的含量高,而传统的处理工艺处理后水的各项污染物达不到排放标准,为此提出了CAF气浮除油-萃取-蒸氨-缺氧-好氧的污水综合处理新工艺,在治理污染的同时回收了焦油、粗笨酚、氨水等化工产品。应用表明,酚氰污水经新工艺处理后,出水完全可以达标排放或回收利用。     

Inhibition of basal and pentagastrin-stimulated gastric acid secretion after treatment with benzilonium bromide and after selective proximal vagotomy

50 consecutive patients with radiographically verified duodenal ulcer and a history of peptic ulcer disease for more than 3 years and with elevated gastric acid secretion in the basal state and after stimulation with pentagastrin were randomly allocated to two groups. The first group of 25 patients was treated with benzilonium bromide (Ulcoban Prolongatum, Parke, Davis & Co.) and the second group was subjected to selective proximal vagotomy (SPV). The patient's gastric acid secretion was determined before the start of treatment and after 1, 6, and 12 months. After one year the basal acid output had decreased by 58.2% in the group treated with benzilonium bromide and by 82.9% in the patients who had undergone SPV. The peak acid output fell by 49.4% in the benzilonium group and by 66.1% in the SPV group.     

Pyridostigmine bromide and Gulf War syndrome

A problem arose when the reamed tibia nail was replaced by a massive unreamed tibia nail. Of the nail fractures, it is difficult to remove the distal fragment from the medullary cavity. It may also happen that the connecting screw between the insertion handle and the nail breaks off at the edge of the implant, which complicates removal of the nail. The authors have developed a technique for removing the implant without having to saw a fissure in the marrow. A concave instrument is slid over the part of the nail left behind after which the two are connected by means of a K wire. This method has been successfully applied in two patients.     

金、银湿法冶炼过程中氮氧化物气体处理工艺的可行性选择

主要介绍了黄金及其它贵金属湿法冶炼过程硝酸分银、王水分金工艺中产生的大量氮氧化物(NOx)气体对人体和周围环境的影响;同时,还介绍了国内外几种氮氧化物气体处理工艺,最终针对黄金及其它贵金属湿法冶炼过程中产生的大量氮氧化物(NOx)气体选择可行性处理工艺.     

由溴丙烷和三正丙胺合成溴化四正丙基胺

溴化四正丙基胺在化学化工方面有着广泛的应用,尤其在合成催化剂时可作为相转移催化剂制备均匀颗粒.本文研究了反应温度、反应时间、不同溶剂对溴化四正丙基胺产率的影响,在80℃反应10 h,以N,N-二甲基甲酰胺做溶剂时,产率最高达到27.50％.红外光谱表明,溴化四正丙基胺需在80℃以下干燥,温度过高会使其分解.     

Ipratropium bromide protects against bronchoconstriction during bronchoscopy

The in vitro activity of fleroxacin was compared to that of ciprofloxacin, ofloxacin and three broad-spectrum beta-lactams against 159 clinical isolates using the E-Test. Fleroxacin, ciprofloxacin, and ofloxacin were all active against Enterobacteriaceae with 93, 98 and 94% susceptibility, respectively. For other gram-negative strains, 81% were susceptible to fleroxacin and ofloxacin and 98% to ciprofloxacin. With regard to gram-positive strains, 72% were susceptible to fleroxacin, 83% to ciprofloxacin and 78% to ofloxacin. The broad antibacterial activity suggests that fleroxacin may be an alternative in the treatment of various infections, particularly in light of its favorable pharmacokinetics.