高效液相色谱法测定肉中五种磺胺类药物残留

采用反相高效液相色谱法，同时测定肉中五种磺胺类药物残留。用瞄柱、紫外检测器，流动相为甲醇＋乙腈＋2％乙酸；9＋18＋73，流速1．0ml／min，柱温25℃，于270nm波长处检测。最低检测限达到5μg／kg，相关系数在0．1-2μg／ml范围内为0．9999，回收率在85．5％-96．6％。     

HPLC法测定鳗鱼中三种喹诺酮药物残留

目的建立一种高效液相色谱方法测定鳗鱼中环丙沙星、恩诺沙星和恶喹酸3种喹诺酮类药物残留量,方法试样用含1％乙酸的乙腈提取,用正己烷脱脂、浓缩,缓冲盐＋乙腈（80＋20,体积分数）溶解,超高速离心净化,用荧光检测器测定,外标法峰面积定量。结果试样在10、0～60．0μg／kg范围内校正曲线呈良好的线性关系,3种物质的相关系数都在0．999以上。加标回收率：恶喹酸为87．4％～105．1％,环丙沙星为70．0％～85．7％,恩诺沙星为76.0％～92．8％,相对标准偏差均小于10％;检出限：恶喹酸为1.4μg／kg,环丙沙星为2．7μg／kg,恩诺沙星为1．5μg／kg。结论该方法操作简单、快捷．结果准确、可靠。     

液相色谱-串联质谱同时测定婴幼儿奶粉中5种四环素类抗生素残留

本文建立了婴幼儿配方奶粉中5种四环素类抗生素残留量的高效液相色谱-串联四极杆质谱联用测定方法。该方法用0．005mol／LNa2EDTA水溶液提取样品,以ZORBAX Aq-C18柱（2．1×150mm,5μm）分离,流动相为乙腈和0．1％甲酸水溶液（梯度洗脱）,电喷雾正离子MRM模式检测。该方法的检出限0．5ng／ml～1．0ng／ml,方法定量下限5．0μg／kg～10μg／kg,线性范围0．5ng／ml～20．0ng／ml,加标回收率84．2％～92．3％,相对标准偏差为2．20％～4．07％。     

液相色谱-电喷雾质谱同时测定肉类中的莱克多巴胺与克伦特罗

建立了液相色谱-质谱法（LC/MS）同时测定肉类中莱克多巴胺与克伦特罗残留的方法。用乙腈-盐酸溶液提取试样中的莱克多巴胺与克伦特罗，旋转蒸干，用甲醇溶解后进行液相色谱-质谱法测定。肉类中莱克多巴胺与克伦特罗的定量检出限均分别为0．2μg／kg与0．5μg／kg。加标回收率分别为82.0％～87.5％与83．6％～89．2％。批内相对标准偏差均小于7．1％。该方法灵敏、定量准确，可广泛应用于肉类中莱克多巴胺与     

LC-APCI-MS同时检测猪肉中7种磺胺类药物残留

为检测猪肉中磺胺类药物残留，建立了猪肉中7种磺胺类药物残留的LC—APCI—MS同时测定方法；样品经过匀浆、酸性甲醇超声波提取、柱净化后进行液相色谱-大气压化学电离-质谱分析；采用Zorbax XDB C18色谱柱，以均含0．2％乙酸的水和甲醇为流动相进行梯度洗脱，外标法定量；7种磺胺类药物的加标回收率为61，0％～114．7％（添加水平为1μg／kg，5pg／kg，10μg／kg），方法精密度1.5％～15.3％，方法检出限为3.92μg／kg～4.20μg／kg。     

高效液相色谱法同时检测调味品中的苏丹红和对位红

建立了调味品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ和对位红的同时检测方法。样品用正己烷提取，提取液用氧化铝层析柱净化。采用Zorbax SB C18柱分离，以95％甲醇为流动相，流速1mL／min，检测波长505nm。上述5种色素组分在其质量浓度为0．16～2．56mg／L时有良好的线性关系，方法的检测限为17～69μg／kg；平均加标回收率为85％～125oA，相对标准偏差为2．6％～6．7％。该方法灵敏可靠，适合于调味品中苏丹红与对位红的同时检测。     

鸡肉中多种喹诺酮类兽药残留量的高效液相色谱测定研究

建立了一种测定鸡肉中氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星兽药残留的高效液相色谱法。样品经乙腈提取，正己烷液一液分配去除蛋白和脂类物质，旋转蒸发除去乙腈，乙腈与水溶解残渣，过微孔滤膜，采用高效液相色谱荧光检测，外标法定量。氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星在0．05～1．2μg／ml浓度范围内线性良好，相关系数为0．9997～0．9999。在0．05～1．0mg／kg浓度范围内，平均加标回收率在78．3％～101．8％之间，相对标准偏差为3．89％～12．43％。方法的最低检出限氧氟沙星为0．0037mg／kg、诺氟沙星为0．0037mg／kg、环丙沙星为0．0035mg／kg、恩诺沙星为0．0022mg／kg。该方法简便、快速，可满足鸡肉中多种唆诺酮类兽药残留量的检测。     

高效液相色谱测定猪肉中醋酸美仑孕酮的残留

建立了高效液相色谱测定猪肉样品中新型兽药醋酸美仑孕酮残留的方法。样品用V（甲醇）：V（0．1％H3PO4）=70：30混合溶液提取,经离心后用PCX固相萃取小柱净化,以Symmetry C8色谱柱为分离柱,线性范围为10～500μg／kg,检出限为5．0μg／kg,平均回收率为93．1～105．5％,相对标准偏差为1．5～4．6％,批间相对标准偏差为1．8～5．6％,检测效果良好。     

高效液相色谱-串联四极杆质谱联席测定饼干中丙烯酰胺残留量

建立饼干中丙烯酰胺残留的高效液相色谱-串联四极杆质谱联用测定方法。该方法经纯水提取样品,以ZORBAXSB-Aq柱（4．6×150mm,5μm）分离,流动相为0．1％甲酸水溶液和乙腈（体积比为97：3）,电喷雾正离子MRM模式检测。该方法的检出限0．3μg／L,方法定量下限3．0μg/kg,线性范围0．3μg,/L-100．0μg/L,加标回收率91．5％～99．5％．相对标准偏差为4．44％。     

Seasonings by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

建立了超高效液相色谱-串联质谱／质谱法高通量快速测定调料中吗啡、可待因、蒂巴因、罂粟碱、那可丁含量。样品通过乙腈一盐酸溶液提取,MCX固相萃取柱净化预处理后用超高效液相色谱-串联质谱／质谱仪进行检测。流动相为0．1％的乙酸-乙腈溶液＋10mmol／L乙酸铵溶液,梯度洗脱,流速为0．2mL／min,柱温40℃。该方法对调料中罂粟碱、那可丁最低检出限分别为O．8、1．0μg／kg,吗啡、可待因和蒂巴因的最低检出限为50μg／kg,8min就可以全部检出。     

Reliable identification of grapevine rootstock varieties using RAPD PCR on woody samples

Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.     

Aroma characterization of various apricot varieties using headspace–solid phase microextraction combined with gas chromatography–mass spectrometry and gas chromatography–olfactometry

The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.     

Tests of potential functional barriers for laminated multilayer food packages. Part I: Low molecular weight permeants

The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.     

Chlorohydrins of bisphenol A diglycidyl ether (BADGE) and of bisphenol F diglycidyl ether (BFDGE) in canned foods and ready-to-drink coffees from the Japanese market

BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H₂O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.     

European priorities for research to support legislation in the area of food contact materials and articles

A strong science base is required to underpin the planning and decision-making process involved in determining future European community legislation on materials and articles in contact with food. Significant progress has been made in the past 5 years in European funded work in this area, with many developments contributing to a much better understanding of the migration process, and better and simpler approaches to food control. In this paper this progress is reviewed against previously identified work-areas (identified in 1994) and conclusions are reached about future requirements for R&D to support legislation on food contact materials and articles over the next 5 or so years.     

Analysis of benzo[a]pyrene in spiked fatty foods by second derivative synchronous spectrofluorimetry after microwave-assisted treatment of samples

A simple, rapid and inexpensive method has been developed for the determination of benzo[a     

The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. II: Certification study

This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the