MBD2和SOCS3调控Th17细胞与重症哮喘发病机制

支气管哮喘是一种常见的、多发的慢性气道炎症性疾病,部分哮喘患者控制不佳进展成为重症哮喘。研究显示辅助性T细胞17(Th17)及白细胞介素17(IL-17)参与以气道中性粒细胞浸润为特点的哮喘发病过程,并且与哮喘的严重度呈正相关。甲基CpG结合蛋白2(MBD2)能通过调节树突细胞中的基因表达程序间接影响CD4+T细胞成熟,促进Th17细胞分化及IL-17产生。细胞因子信号传导抑制蛋白3(SOCS3)通过对贾纳斯激酶(Janus kinase)信号传导及转录激活因子途径(JAK/STAT)负性调节而抑制信号转导,从而抑制Th17细胞分化。MBD2与SOCS3启动子区的CpG岛结合,可导致该位点的甲基化,从而调控Th17细胞分化参与重症哮喘的发病。     

应用AG490阻断STAT3对系统性红斑狼疮Th17/Treg平衡的调控研究

目的:研究应用免疫抑制剂AG490阻断信号传导与转录活化因子3(STAT3)对系统性红斑狼疮(SLE)患者辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的调控。方法:选取本院于2018年10月-2019年6月收治的10例SLE(观察组)以及10例体检健康者(对照组),抽取所有受试者外周血约10 mL,采用免疫磁珠法提取CD4+T细胞,并用流式细胞术检测所有受试者Th17/Treg细胞亚群变化。用AG490(0、25、50、100μM)处理患者CD4~+T细胞,检测Th17/Treg细胞亚群以及细胞因子变化,并采用Q-PCR了解STAT3、Foxp3、RORγt水平。结果:观察组患者Th17显著高于对照组,而Treg水平显著低于对照组,两组患者Th17/Treg比较,差异有统计学意义(P<0.05)。随着AG490浓度的增加,Th17/Treg比值、IL-6、IL-17表达水平均逐渐降低,而IL-10、TGF-β水平均显著增加,各组间比较差异均有统计学意义(P<0.05)。经过不同浓度AG490处理后,STAT3、RORγt mRNA水平以及STAT3蛋白水平均显著降低,其中100μM剂量组蛋白水平显著低于其他三组,而Foxp3 mRNA水平显著增加,各组mRNA水平比较,差异均有统计学意义(P<0.05)。结论:通过阻断STAT3信号通路,可诱导Treg分化以及抑制Th17分化,从而调控Th17/Treg平衡,可为系统性红斑狼疮的治疗提供新方向。     

Th17 细胞相关细胞因子与肿瘤简

辅助性T细胞17（Th17）是一种不同于Th1和Th2的新型CD4＋T细胞亚群。Th17细胞的相关因子,如白细胞介素-6（IL-6）、IL-17及 IL-23等,都在肿瘤的发生发展中起着重要的作用。探讨Th17细胞相关细胞因子与肿瘤的关系,将有助于对肿瘤的发病机制的进一步了解,并为恶性肿瘤的治疗提供新的靶点。     

NRP-1参与Tregs免疫调节作用机制的研究进展

NRP-1在Tregs发挥免疫调节功能中扮演着重要角色,因此深入研究NRP-1与Tregs之间的相互作用对研究相关疾病的发生发展起到至关重要的作用。本文就NRP-1及Tregs的生物学特性,NRP-1在Tregs上的表达及两者之间的作用机制3个方面做一综述。     

槲皮素对人卵巢癌细胞-3增殖和凋亡的影响

目的:探讨槲皮素对人卵巢癌细胞-3(ovarian carcinoma cell,OVCAR-3)增殖和凋亡的影响及其作用机制。方法:将对数生长期的OVCAR-3细胞随机分为对照组和槲皮素组。对照组细胞给予培养基常规培养,而槲皮素组细胞加入槲皮素(160μmol·L~(-1))。各组细胞培养48 h后,利用噻唑蓝检测人卵巢癌细胞株OVCAR-3细胞增殖;流式细胞仪检测人卵巢癌细胞株OVCAR-3细胞凋亡,RT-PCR检测细胞受体型酪氨酸激酶2(JAK2),信号传导与转录激活因3(STAT3),B细胞淋巴因子2(Bcl-2),人Bcl-2相关X蛋白(Bax)信使mRNA表达水平。免疫蛋白印记法检测各组OVCAR-3细胞中STAT3,磷酸化受体型酪氨酸激酶2(p-JAK2),磷酸化信号传导与转录激活因3(p-STAT3),Bax,Bcl-2蛋白的变化,Caspase3活性试剂盒检测Caspase3活性。结果:MTT检测结果显示槲皮素组与对照组相比,人卵巢癌细胞株OVCAR-3细胞增殖率明显下降(P<0.05)。流式细胞仪检测显示槲皮素组OVCAR-3细胞凋亡率较对照组明显升高(P<0.05)。RT-PCR检测显示槲皮素组与对照组JAK2和STAT3信使mRNA表达水平无明显差异,而槲皮素组Bax信使mRNA水平比对照组明显增高,槲皮素组Bcl-2信使mRNA水平比显著低于对照组。Western blotting检测显示,槲皮素组的JAK2和STAT3蛋白表达与对照组比较,无明显差异(P>0.05),而槲皮素组的p-STAT3,p-JAK2和Bcl-2蛋白表达水平显著低于对照组(P<0.05),槲皮素组的Bax表达显著高于对照组(P<0.05)。Caspase3活性试剂盒检测结果显示,槲皮素组Caspase3活性表达水平显著高于对照组。结论:槲皮素能明显抑制人卵巢癌细胞株OVCAR-3细胞增殖,促进人卵巢癌细胞株OVCAR-3细胞凋亡,其作用机制可能是通过激活JAK2/STAT3信号传导通路。     

细胞因子信号转导抑制分子3与肿瘤关系的研究进展

细胞因子与相应的受体结合可导致信号传导通路的活化,而将细胞因子的信号由胞膜传到胞浆并最终传至胞核,并引起目的基因的表达。已经发现的10余条细胞因子信号传导通路,其中Janus激酶/信号转导和转录激活子（JAK/STAT）系统涉及细胞因子的范围较为广泛。过去研究细胞因子信号的传入较多,较少观察这些信号是如何中止或衰减。     

肤悦康洗剂联合补脾祛风方对特应性皮炎患者Th17、IL-17和IL-23的影响

目的:观察肤悦康洗剂联合补脾祛风方治疗特应性皮炎的临床疗效及其对特应性皮炎辅助性T细胞17(T helper cell 17,Th17)、白细胞介素-17(interleukin-17,IL-17)及白细胞介素-23(interleukin-23,IL-23)细胞通路的影响。方法:使用随机、对照、单盲的方法,将入选115例特应性皮炎患者分为治疗组60例和对照组55例,治疗组采用肤悦康洗剂(苦参、白矾、土槿皮、地肤子、白鲜皮、荆芥、广东紫荆皮)湿敷患处联合补脾祛风方(党参20g,白术10g,炙甘草10g,黄芪20g,白蒺藜15g,茯苓15g,防风10g,蝉蜕10g)内服治疗;对照组外用尿素乳膏联合口服复方甘草酸苷片+氯雷他定片治疗。两组患者均治疗2周。观察两组患者特应性皮炎严重程度评分指数(scoring atopic dermatitis index,SCORAD)积分变化情况,并检测患者外周血及皮损中Th17、IL-17及IL-23水平。结果:治疗组在治疗后SASSAD积分下降,与本组治疗前比较及治疗后两组患者比较,差异均有统计学意义(P<0.01);治疗组患者治疗后外周血及皮损中Th17、IL-17及IL-23水平明显下降,与同组治疗前比较,且与两组患者治疗后比较,差异均有统计学意义(P<0.01)。结论:肤悦康洗剂联合补脾祛风方治疗特应性皮炎可显著改善患者临床症状,降低皮损SASSAD积分,降低患者外周血及皮损中Th17、IL-17及IL-23水平。     

土动力学与土工抗震研究进展综述

综述当前国内外土动力学与土工抗震方面的研究进展,包括土体动力特性与本构关系、土工地震反应分析、土体动力测试、土体振动液化、土体地震永久变形、交通荷载作用下土体动力特性和土工抗震措施等内容。对各种方法的优缺点进行比较和评述。进一步阐述今后的研究方向。     

软土地下工程与深基坑研究进展

针对当前国内外软土地下工程研究现状进行系统概括和评述,重点探讨深基坑和盾构隧道两种典型地下工程涉及的关键土工问题。其一是复杂地质及环境条件下软土基坑工程的分析方法与新技术,包括基坑围护结构受力分析、基坑抗隆起稳定性分析、渗流对基坑稳定性的影响、深基坑工程中的冗余度问题、软土基坑工程的新技术;其二是软土隧道工程的稳定与变形问题,包括隧道开挖面稳定性分析、渗流对隧道开挖面稳定性的影响、隧道纵向不均匀沉降与长期沉降、隧道地震响应分析与动力模型试验;最后是软土地下工程施工的环境土工效应,包括基坑开挖引起的土体变形、隧道开挖引起的土体变形、地下工程开挖对周围环境的影响。     

Computational methods for fracture in rock: a review and recent advances

We present an overview of the most popular state-of-the-art computational methods available for modelling fracture in rock. The summarized numerical methods can be classified into three categories: Continuum Based Methods, Discrete Crack Approaches, and Block-Based Methods. We will not only provide an extensive review of those methods which can be found elsewhere but particularly address their potential in modelling fracture in rock mechanics and geotechnical engineering. In this context, we will discuss their key applications, assumptions, and limitations. Furthermore, we also address ‘general’ difficulties that may arise for simulating fracture in rock and fractured rock. This review will conclude with some final remarks and future challenges.     

结构损伤检测方法的一些新进展

综述了结构损伤检测的方法。分别按照结构损伤模式、损伤检测中所研究的结构类型、损伤检测所使用的力学理论和数学方法将损伤检测法进行分类,对结构损伤检测的各种途径进行了简要介绍,此外,还叙述了各种方法的研究现状及适用范围。最后,对损伤检测中存在的一些问题提出了一些观点。     

A critical analysis of recent advances in the techniques for the evaluation of renewable energy projects

Renewable energy investment is an integral part of sustainable economic development agenda. Whereas some important advances have been made in recent years to assist project investors in making better use of financial risk management instruments and taking into consideration real options embedded in renewable energy projects, this research asserts that, owing to failure to consider both behavioural uncertainty and the limit of risk transfer, these approaches may still lead to a biased evaluation result. Drawing on a novel concept of “risk-bearing capacity”, the research suggests developing a new approach whereby investors can incorporate the choice of financial protection measures into investment evaluation in a coherent way.     

Sustainability in the construction industry: A review of recent developments based on LCA

This review brings together research on life cycle assessment (LCA) applied within the building sector. More than ever, the construction industry is concerned with improving the social, economic and environmental indicators of sustainability. By applying LCA it is possible to optimise these aspects, from the extraction of raw materials to the final disposal of waste building materials. Firstly, this review details LCA concepts and focuses on the LCA methodology and tools employed in the built environment. Secondly, this paper outlines and discusses the differences between the LCA of building materials and components combinations versus the LCA of the full building life cycle. Finally, this work can be used by stakeholders as an important reference on LCA including up to date literature on approaches and methodologies to preserve the environment and therefore achieve sustainable development in both developed and developing countries.The present review has tried to compile and reflect the key milestones accomplished in LCA over the last 7 years, from 2000 to 2007 within the building sector. In summary, it can be stated that the application of LCA is fundamental to sustainability and improvement in building and construction. For industrial activities, SMEs must understand the application of LCA, not only to meet consumer demands for environmentally friendly products, but also to increase the productivity and competitiveness of the green construction markets. For this reason, this review looks at LCA because of its broad international acceptance as a means to improve environmental processes and services, and also for creating goals to prevent adverse environmental impacts, consequently enhancing quality of life and allowing people to live in a healthy environment.     

西施舌增养殖的研究现状与展望

介绍了西施舌Coelomactraantiquata在中国的增养殖研究现状,并对其进一步发展中应解决的关键技术问题进行了探讨,提出了应研究建立室内立体附苗与稚贝立体培育技术、重点攻克大规格苗种中间培育技术及优化增养殖技术措施等观点,同时对其发展前景进行了展望。     

What should housing vouchers do? A review of the recent literature

This paper challenges the assumption that housing vouchers help recipients move from crime-ridden inner-city areas to ‘good’ lower-poverty neighborhoods and, in turn, to achieve self-sufficiency. A review of the American and European literature on housing mobility programs and on mixed-income communities fails to support this belief. Efforts to maintain, and expand, the voucher program should be based on the program’s proven ability to provide decent and affordable housing rather than on unproven claims that it promotes poverty deconcentration and family self-sufficiency. I find considerable evidence that voucher recipients cluster spatially and that this promotes social decline in neighborhoods already vulnerable to change. Policymakers need to monitor voucher settlement patterns and to address programmatic weaknesses that cause negative neighborhood effects and voucher controversies.     

河南省农民体育健身工程现状与对策研究

采用文献资料、调查、分析等方法,对河南省农民体育健身工程实施现状进行了调查分析,发现在农民体育健身工程实施中存在着硬件不硬、软件太软以及发展的外部环境差等问题。据此提出了大力发展农村经济和农村教育,积极开展对农体育宣传,传播体育观念和风尚,加大农村体育基础设施建设力度,加强农村体育组织和人才队伍建设,广泛开展群众性的农村体育竞赛与活动等,提出河南省"农民体育健身工程"建设的对策与建议。     

扁平苔藓中Th17细胞相关因子研究进展

扁平苔癣(Lichen Planus,LP)是一种慢性炎症性皮肤病,其特征性的病理改变为基底膜带的液化变性及真皮浅层淋巴细胞的带状浸润。Th17细胞是近年来发现的一种辅助性细胞,它是由CD4~+T细胞在一些细胞因子的刺激下分化而来。近年来大量研究表明Th17细胞相关因子如IL-17、IL-22、IL-23在LP病变处高表达,它们在介导损伤部位炎细胞浸润及基底膜带的破坏都有重要的作用。本文就Th17细胞相关因子与LP的关系作一综述。