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1.
Microbiological and some physical and chemical effects of treating pork chop surfaces with sodium acid pyrophosphate, a commercial phosphate blend, potassium sorbate and phosphate/sorbate/sodium acetate solutions, with or without sodium chloride, before packaging were studied in pork chops vacuum-packaged and stored at 2–4°C for 10 weeks. All treatments containing potassium sorbate reduced (P<0.05) counts of mesophiles, psychrotrophs, EnterobacteriaCeae, facultative anaerobes, and lactobacilli. Treatment of chops with 10% phosphates/ 10% potassium sorbate solutions improved pork color and decreased purge. Potassium sorbate alone reduced microbial counts more than it did when combined with phosphates, but chops were darker and had more exudate (P<0.05). Combined use of 10% phosphates/10% potassium sorbate extended shelf life in vacuum-packaged fresh pork chops to 10 weeks at 2–4°C compared with 4 weeks for untreated pork and protected meat color.  相似文献   

2.
Combinations of sodium acid pyrophosphate (SAPP) with added sodium nitrite and/or potassium sorbate were tested at various pH levels to determine effectiveness in delaying Clostridium botulinum growth and toxin production in frankfurter emulsions. Formulations containing sodium nitrite (40 ppm), potassium sorbate (0.26%) and SAPP (0.4%) resulted in a greater delay of toxin production (12–18 days) than other combinations (6–12 days) having similar pH values. Treatments containing 0.4% SAPP appeared to be more inhibitory than their counterparts without SAPP, displaying less numbers of toxic samples during the 53-day storage period at 27°C. Aerobic mesophilic colony counts and residual nitrite data showed little difference among treatments.  相似文献   

3.
Enhancement of beef biceps femoris muscles (n=45) with solutions comprising sodium hexametaphosphate (SHMP), sodium tripolyphosphate (STPP), or tetrasodium pyrophosphate (TSPP) at either 0.2% or 0.4% of product weight, with the exclusion of sodium chloride, was performed to observe the independent phosphate effects on instrumental color during simulated retail display. All solutions were injected into muscle samples at either 112% (12% pump) or 118% (18% pump) of raw product weight. All three phosphate types maintained higher (P<0.05) L* values than untreated steaks (CNT) through 5 days-of-display, and SHMP had higher (P<0.05) L* values than STPP and TSPP through 7 days-of-display. Additionally, steaks with 0.2% phosphate inclusion were lighter (L*; P<0.05) than CNT throughout display, and were lighter (P<0.05) than steaks enhanced with 0.4% phosphates through 7 days of display. Steaks enhanced with TSPP had higher (P<0.05) a* values than CNT on days 5 and 7 of display, whereas SHMP- or STPP-enhanced steaks generally had similar (P>0.05) a* values as CNT after 3d of display. Direct comparison of phosphate concentrations revealed no differences (P>0.05) in a* values. Only steaks enhanced with TSPP were more vivid (P<0.05) and had higher (P<0.05) proportions of oxymyoglobin than CNT on days 5 and 7 of display. However, direct comparison of phosphate types indicated that TSPP- and STPP-enhanced steaks had similar (P>0.05) oxymyoglobin proportions during display. Phosphate inclusion at 0.4% maintained higher (P<0.05) oxymyoglobin proportions than 0.2% phosphate inclusion through 5 days-of-display. These results indicate that while 0.2% phosphate concentrations maintain lighter color, 0.4% concentrations can more effectively retain oxymyoglobin during display. Additionally, only steaks enhanced with TSPP were redder, more vivid, and had higher oxymyoglobin proportions than untreated steaks during the latter stages of display.  相似文献   

4.
Sodium benzoate,potassium sorbate and sodium nitrite as silage additives   总被引:1,自引:0,他引:1  
BACKGROUND: The hygienic quality of silage is important for animal health, animal production and food quality. The aim of this study was to evaluate the effect of five silage additive mixtures on yeast, mould, and clostridia growth and other silage quality criteria. The additives, composed of sodium nitrite, sodium benzoate and/or potassium sorbate, were used to treat a grass forage crop before ensiling. The silages were compared with untreated silage and silages treated with two commercial additives: Promyr NF (PNF), which is based on formic and propionic acid, and Kofasil Ultra (KU), a mixture of sodium nitrite, sodium benzoate, sodium propionate and hexamine. RESULTS: All tested additives significantly (P < 0.001) reduced butyric acid and ammonia‐N formation in low‐dry‐matter (DM) silages compared to the control. Only KU and the mixtures of 600 g sodium nitrite, 250 g sodium propionate, 750 g sodium benzoate (A1) and 250 g sodium nitrite with 1000 g sodium benzoate and 500 g potassium sorbate (A5) ton?1 of fresh forage were found to reduce (P < 0.001) the presence of clostridia spores in low‐DM silages. In high‐DM silages, treatments A1, A5 and A2 (250 g sodium nitrite and 1000 g sodium benzoate) contained less (P < 0.02) yeast than the control and PNF, but not KU. All treated silages were aerobically stable. The concentrations of nitrate‐N in all silages and nitrite‐N in low‐DM were below the threshold level for feed at the end of storage. CONCLUSIONS: The additive mixtures A1 and particularly A5 improved silage quality at least to the same extent as commercial products, even though they contained no hexamine and less nitrite. Copyright © 2009 Society of Chemical Industry  相似文献   

5.
This study determined the effects of potassium lactate (KL), sodium chloride, sodium tripolyphosphate, and sodium acetate on colour, colour stability, and oxidative properties of injection-enhanced beef rib steaks. Enhancement solutions (8.5% pump) contained combinations of KL (0% or 1.5%), sodium chloride (0.3% or 0.6%), sodium tripolyphosphate (0% or 0.3%), and sodium acetate (0% or 0.1%). Steaks were packaged in a high-oxygen modified atmosphere (80% O(2)/20% CO(2)). Steaks with KL or KL and sodium acetate were darker but more colour stable (P<0.05) than control steaks. Steaks had less glossy surfaces when they contained acetate (P<0.05) and KL (P<0.11). Increasing sodium chloride content resulted in darker, less colour-stable steaks (P<0.05). Removing phosphate had little impact on colour (P>0.05). Both KL and sodium acetate improved visual appearance of injection-enhanced beef rib steaks, whereas the greater salt level were detrimental.  相似文献   

6.
The effects of potassium sorbate alone and in combination with 2 or 3% sodium chloride (NaCl) on the growth of Salmonella typhimurium ATCC 7136 were examined. Growth studies were performed in tryptic soy brogh (adjusted to a final pH 6.3) at 22°C for 15 days and 35°C for 48 hr without shaking. A dual plating procedure was employed to monitor growth (tryptic soy agar) and development of injury in the cell population (Levine's eosine methylene blue agar + 2% NaCl + 0.05% sodium desoxycholate). The difference between these two plate counts served as a measure of the number of injured organisms. It was found that combinations of sorbate and NaCl were more effective in the inhibition of S. typhimurium than sorbate alone under the conditions and variables studied. No injury was observed in the cell population as determined by our assay procedure. The combination of 3% NaCl + 0.3% potassium sorbate was the most effective in inhibiting growth of S. typhimurium at either storage temperature.  相似文献   

7.
Experiments were conducted to evaluate and compare the combined effects of two preservatives, potassium sorbate and sodium benzoate, with heat on inactivation and injury of 12 strains of yeasts. There does not appear to be any correlation between ability to sporulate and z value or general heat sensitivity. Heat treatment caused an increase in sensitivities of 10 of the 12 strains to potassium sorbate (≤ 1000 ppm) as evidenced by inability to form colonies in the presence of the preservative in a recovery medium. Heated cells of five test strains had increased sensitivity to ≤ 1000 ppm of sodium benzoate. Whether exposed to potassium sorbate or not during heating, 100 ppm of the preservative in recovery broth severely retarded, and in some instances completely inhibited, growth of yeasts for a period of 10 hr post treatment. These results strongly suggest that yeasts possessing a wide range of physiological characteristics are subject to heat-injury and, furthermore, that postassium sorbate enhances injury during heating and retards or prevents repair of injury during recovery. Sensitivity to heat generally increased as the pH of the heating medium decreased from 7.0 to 2.5. Without exception, the presence of 500 ppm of potassium sorbate or sodium benzoate in the heating medium caused more rapid inactivation rates of yeasts compared to heating in the absence of preservatives. At comparable concentrations, sodium benzoate was less effective than was potassium sorbate, which exerted a significant (P ≤ 0.05) effect on increasing the rate of heat inactivation at concentrations as low as 50 ppm.  相似文献   

8.
ABSTRACT:  Paired USDA Select beef strip loins ( n  = 10), aged 2 d, were injected with either an alkaline-based (3.6% sodium chloride, 1% Herbalox seasoning, adjusted to pH 10 with ammonium hydroxide [approximately 0.1%, FFC grade]) or a phosphate-based (3.6% sodium chloride, 1% Herbalox seasoning, 4.5% sodium tripolyphosphate) brine. Steaks were evaluated for 19 d. Overall, phosphate-injected steaks performed better than alkaline-injected steaks with respect to cook yield, water holding capacity, lipid oxidation, color stability, tenderness, and juiciness. Phosphate-injected steaks also had less purge than alkaline-injected steaks, as confirmed by composition analysis. Phosphate-injected steaks were higher in moisture and ash content, and were nearly 2% lower in protein content. Alkaline-injected steaks had significantly lower aerobic (approximately 1 log lower) and anaerobic (approximately 2 log lower) plate counts. Final meat pH probably contributed to the differences observed between treatments. The final pH of phosphate-injected steak was 5.99 while that of alkaline-injected steak was 5.73. Further research should be conducted to determine the concentration of ammonium hydroxide needed in the alkaline-based brine to increase the final meat pH to similar levels found in the phosphate-injected steaks.  相似文献   

9.
Both sodium benzoate and potassium sorbate were significantly detected (P < 0.05) in prune juice at levels of 500 to 600 parts/million. Detection levels for both preservatives in high-moisture dried prunes were higher (about 800 parts/million). No significant flavour differences were found between prunes treated with similar amounts of sodium benzoate and potassium sorbate. Since it was necessary to use more sodium benzoate than potassium sorbate to preserve prunes, comparisons were made between prunes containing equally effective levels of the two preservatives. No significant flavour differences were found between prunes effectively preserved with sodium benzoate and potassium sorbate. This suggests that sodium benzoate could be used as a preservative for high-moisture dried prunes with essentially no flavour problem.  相似文献   

10.
All beef frankfurters and beef-pork frankfurters containing various levels of sodium nitrite (0, 50, or 156 ppm) or potassium sorbate (0, 0.26, or 0.39%) alone or in combination (50 ppm nitrite + 0.26% sorbate) were prepared. Frankfurters were inoculated with nalidixic acid resistant Salmonella and incubated at 15°C and 27°C for up to 21 days. Frankfurters formulated with 50 or 156 ppm nitrite and incubated at 27°C, or 50 ppm nitrite and incubated at 15°C failed to inhibit the growth of Salmonella. Sorbate alone and sorbate in combination with 50 ppm nitrite were equally effective in inhibiting Salmonella at either temperature and were equivalent to 156 ppm nitrite in inhibiting Salmonella at 15°C.  相似文献   

11.
Sensory, physical, and cooking properties were evaluated on bacon processed under commercial operations in four separate plants. Three formulations with various levels of sodium nitrite and potassium sorbate were used: (1) 0 ppm sodium nitrite, (2) 40 ppm sodium nitrite 2600 ppm potassium sorbate, and (3) 120 ppm sodium nitrite. Sliced bacon was evaluated after 10, 30, 50, and 70 days of storage. “Chemical”-like flavors, prickly mouth sensations, and “sweet aromatic” aromas were found in bacon processed with 40 ppm sodium nitrite and 2600 ppm potassium sorbate. “Microbial” flavors were found in 0 ppm sodium nitrite bacon after 50 and 70 days of storage. Bacon stored for 30 days had less cooking loss and sensory panel scores indicative of more mouth coating with fat than did bacon stored for 10 days. The degree of leanness in bacon strips had more of an influence on textural, physical, and cooking characteristics than did sodium nitrite and potassium sorbate levels.  相似文献   

12.
The effect of sodium phosphates with and without NaCl, on the ability of unstressed and heat-stressed Moraxella-Acinetobacter (M-A) isolate number 7 cells to form colonies in plate count agar (PCA) was determined. The effectiveness, in order of decreasing ability, of phosphates to inhibit colony formation of unstressed M-A cells was as follows: sodium tripolyphosphate (Na5P3O10; STPP), sodium pyrophosphate (Na4P2O7; SPP), and sodium orthophosphate (Na3PO4; SP). Filter sterilized STPP was more inhibitory than when heated and inhibition was not related to pH. Certain combinations of NaCl and STPP were additive whereas combinations of NaCl and SPP exhibited a synergistic effect. Heat-stressed M-A cells were more sensitive to levels of NaCl (0.8%) than to levels of STPP (0.1%) or SPP (0.12%); all shown to have no effect on the number of colonies formed in PCA by unstressed cells.  相似文献   

13.
The effect of enhancing beef biceps femoris muscles (n=45) with solutions comprising 2.0% sodium chloride and either sodium hexametaphosphate (SHMP), sodium tripolyphosphate (STPP), or tetrasodium pyrophosphate (TSPP) at either 0.2% or 0.4% of product weight on instrumental color during simulated retail display was investigated. All solutions were injected into muscle samples at either 112% (12% pump) or 118% (18% pump) of raw product weight. Muscles treated with all three phosphate types had lower (P<0.05) L* and b* values compared to untreated muscles (CNT). Steaks enhanced with STPP had similar (P>0.05) a* values as CNT, whereas SHMP- and TSPP-treated steaks generally had lower a* values than CNT. Across phosphate type, excluding day 3 of display, steaks treated with phosphate at 0.4% had similar (P>0.05) a* values as CNT, whereas those with 0.2% phosphate addition had lower (P<0.05) a* values than CNT. Across five days of display, STPP maintained higher (P<0.05) a* values than steaks treated only with sodium chloride, whereas SHMP did not differ (P>0.05) from sodium chloride-treated steaks. While STPP maintained a similar (P>0.05) saturation index as CNT, SHMP and TSPP generally had decreased (P<0.05) vividness during display. Additionally, excluding day 3 of display, phosphate concentration at 0.4% maintained similar vividness as CNT, whereas 0.2% phosphate concentration caused decreased (P<0.05) vividness, compared to CNT. The 630/580nm ratio results indicated that SHMP had less (P<0.05) oxymyoglobin than CNT throughout display. Disregarding day 3 of display, both STPP and TSPP had similar (P>0.05) oxymyoglobin proportions as CNT. These results indicate that STPP was the most effective phosphate type for maintaining color. Additionally, 0.4% phosphate concentrations can maintain color better than 0.2% phosphate concentrations. However, none of the phosphate/salt combinations produced superior color, compared to untreated steaks.  相似文献   

14.
Survival and growth of inoculated Clostridium sporogenes PA3679 and natural aerobic and anaerobic bacterial flora were studied in refrigerated (5°C) and subsequently temperature abused (24–25°C), cooked, vacuum-packaged bratwurst containing 0.5% sodium acid pyrophosphate (SAPP) or sodium tripolyphosphate (STPP) with or without sodium nitrite. Phosphates alone or combined with nitrite did not affect aerobic bacterial counts but resulted in reduced clostridial and anaerobic counts at 5°C. Upon temperature abuse, inhibition of all bacteria by SAPP was significant (P<0.05) for up to 48 hr and greatly enhanced by 100 ppm but not by 50 ppm sodium nitrite, whereas STPP lost its antimicrobial properties after 24 hr. Soluble orthophosphate levels had a positive correlation with bacterial inhibition in SAPP-treated bratwurst.  相似文献   

15.
Select beef loin pairs (n = 10) were injected (10% pump) with brine containing either 4.5% sodium-based phosphates, (CON), or 1% ammonium hydroxide treatment (AHT). Both brines also contained 3.6% NaCl and 1% Rosemary Herbalox. Steaks cut from loins were high oxygen (80% O(2)/20% CO(2)) modified atmosphere packaged, stored 4 d at 4 °C in the dark to simulate transportation, and then placed in retail display for 14 d (4 °C). On day 0, 7, and 14 of retail display steak properties were measured. Purge from AHT steaks was higher than CON (P < 0.05). Panelists were not able to visually discriminate between AHT and CON steaks through the first 6 d of retail display. After day 6, panelists rated AHT steaks higher for muscle color, percent discoloration, and overall color. Steaks from both treatments started at day 0 retail display with similar total plate counts (P > 0.05). Microbial counts increased more rapidly for AHT steaks than CON steaks (P < 0.05). AHT and CON steaks were not different in terms of lipid oxidation through day 7 retail display. By day 14 retail display CON steaks were above the threshold for consumer perception of oxidized flavors in fresh meat. However, results also indicated the AHT and CON steaks were no longer acceptable by day 14 in terms of color, were questionable in terms of microbial load, and likely were beyond their reasonable shelf life. Based on retail display properties, results indicated 1% AHT could successfully replace 4.5% SP in a meat injection brine. Practical Application: The research in this report compares steaks that have been injected with a commercial brine formulated with SP to steaks that have been injected with a brine, where the SP in the formulation are replaced with 1% AHT. Ammonium hydroxide is an USDA-FSIS approved ingredient in brines injected into fresh meats. Successful replacement of sodium phosphate with ammonium hydroxide would allow processors to significantly reduce the sodium content of injected fresh meat.  相似文献   

16.
The effects of sodium acid pyrophosphate (SAPP), sodium chloride (NaCl) and/or potassium sorbate (PS) on the growth from heat-activated spores of three individual strains or a mixture of ten strains of Clostridium botulinum in peptone-yeast extract-glucose broth at pH 5.55 or 5.85 were measured spectrophotometricalty at A630nm. Growth ratios (GR = treatment/control) based on time to reach A630= 0.35 or 0.04 were calculated and used to compare effects of additives on strains. SAPP, NaCl, PS, and pH exhibited independent significant main effects (p≦0.01) on delaying growth in most C. botulinum strains tested. Combinations of additives without NaCl consistently caused an increase in the GR and an increase in organism sensitivity to additives in the medium. Treatments containing SAPP (0.2 or 0.4%) and PS (0.13 or 0.26%) were more effective for delaying growth than other formulations tested.  相似文献   

17.
This study investigated the effect of mixing and grinding, tempering, addition of sodium chloride and sodium tripolyphosphate and processing under a nitrogen atmosphere on the color of restructured beef steaks initially and at 1-month intervals for 3 months of frozen storage. Mixing and grinding and manufacture under a nitrogen atmosphere had the greatest detrimental effect on the extent of discoloration initially; this was probably due to low oxygen concentration. Tempering and the addition of sodium chloride without sodium tripolyphosphate produced the most rapid increase in rate of discoloration during frozen storage. Tripolyphosphate partially counteracted the detrimental effect of sodium chloride, however, this effect did not appear to be due to the higher pH produced by the phosphate or the chelating ability of the phosphate. Surface discoloration and overall color were highly correlated with surface metmyoglobin (r= -0.87) and overall met-myoglobin (r= -0.94).  相似文献   

18.
Beef biceps femoris muscles (n=45) were used to evaluate the effect of enhancement with solutions comprising 2.0% sodium chloride and either sodium hexametaphosphate (SHMP), sodium tripolyphosphate (STPP), or tetrasodium pyrophosphate (TSPP) at either 0.2% or 0.4% of product weight. All solutions were injected into muscle samples at either 112% (12% pump) or 118% (18% pump) of raw product weight. Muscles treated with all three phosphate types had decreased (P<0.05) free water compared to untreated muscles (CNT), and while TSPP-treated muscles were able to bind greater (P<0.05) additional water than CNT, STPP- and SHMP-treated muscles did not differ (P>0.05) from CNT. Disregarding phosphate type, steaks with 0.4% phosphate inclusion bound more (P<0.05) water than those with 0.2% phosphate inclusion. Steaks treated with STPP or TSPP had decreased (P<0.05) cooking losses than CNT, while SHMP-treated steaks did not differ (P>0.05) from CNT. Steaks injected at 18% pump had greater (P<0.05) percent moisture, and did not differ (P>0.05) in free water, water binding, or cooking losses from steaks injected at 12% pump. Although there were no differences (P>0.05) in Warner-Bratzler shear force in this study, steaks with SHMP, STPP, and TSPP all were rated more tender, and juicier (P<0.05) by sensory panelists than CNT steaks or steaks enhanced only with sodium chloride. Regardless of phosphate type, steaks enhanced with 0.4% phosphate and those steaks at 18% pump received improved (P<0.05) sensory tenderness ratings compared to 0.2% phosphate and 12% pump, respectively. These results suggest that enhancing biceps femoris muscles with STPP or TSPP can improve water retention, yield, and palatability characteristics. Additionally, enhancement with a phosphate/salt solution at an 18% pump rate, compared to a 12% pump rate, can allow for improved sensory tenderness perceptions without decreasing product yields.  相似文献   

19.
Beef triceps brachii muscles (6 d postmortem; n= 15; muscle sections n= 45) were sectioned into 3rds and allocated to 1 of 3 treatments. The treatments were untreated (CNT), or injected at a 12% pump rate with either tap water‐only (H2O) or a solution comprising tetrasodium pyrophosphate and sodium chloride (TSPP/ NaCl) at 0.4% and 1.0% target final product weight concentrations, respectively. Each muscle (comprising all 3 treatments) was then allocated to 2, 14, or 28 d of vacuum‐packaged 1°C storage. Purge losses during storage were greatest (P < 0.05) for H2O muscles and least (P < 0.05) for TSPP/NaCl muscles. Purge losses also increased (P < 0.05) from 2 d to 14 d of storage. Steaks enhanced with TSPP/NaCl had less (P < 0.05) free water and lower (P < 0.05) cooking losses than either CNT or H2O steaks. Storage duration did not affect (P > 0.05) Warner‐Bratzler shear force (WBS) or sensory tenderness, but juiciness decreased (P < 0.05) with increased storage duration. While storage duration did not impact (P > 0.05) instrumental color characteristics, aerobic plate counts generally increased during storage. The TSPP/NaCl steaks had lower (P < 0.05) WBS values and improved (P < 0.05) sensory tenderness and juiciness characteristics compared with CNT or H2O steaks. While CNT steaks had greater (P < 0.05) L* values (lightness) than TSPP/NaCl steaks, TSPP/NaCl steaks had similar (P > 0.05) oxymyoglobin proportions (630/580 nm) and a* values (redness) as CNT steaks. These results suggest enhancement with TSPP/NaCl can improve triceps brachii yield and palatability characteristics. Increased post‐enhancement storage did not impact or worsened palatability while increasing purge losses, suggesting general deleterious effects of increased postmortem storage for this muscle.  相似文献   

20.
Uncured turkey and chicken breast meat products, formulated with NaCl (0.65 and 1.30%) and potassium sorbate (0 and 0.26%), were tested for antimicrobial activity and functional properties. The chopped products were inoculated (Clostridium sporogenes, 10/g) in cans and in packages and abused at 20°C. Microbial growth and gas production were rapid at both NaCl levels tested. Inclusion of potassium sorbate in the formulation delayed initiation and rate of microbial growth and gas production. Potassium sorbate also reduced (P<0.05) weight losses during cooking of the low NaCl turkey product. Rates of gas production were similar in canned and vacuum packaged products. Differences in microbial growth and gas production between chicken and turkey products were minor.  相似文献   

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