Sensitivity to UV radiation in early life stages of the Mediterranean sea urchin Sphaerechinus granularis (Lamarck)

The sea urchin Sphaerechinus granularis was used to investigate the impact of relevant levels of UV-B radiation on the early life stages of a common Mediterranean free spawning benthic species. Sperm, eggs and embryos were exposed to a range of UV radiation doses. The resulting endpoints were evaluated in terms of fertilisation success, development and survival rates. Above a weighted UV radiation dose of 0.0029 kJ m^− 2, fertilisation capability of irradiated sperm decreased rapidly. The exposure of the eggs to 0.0175 kJ m^− 2 and more led to delayed and inhibited development with ensuing embryonic morphological abnormalities. One-day old larvae remained strongly sensitive to UV radiation as shown by the 50% decrease of the larval survival rate for a dose of 0.025 kJ m^− 2 UVR. The elevated sensitivity of embryos to experimental UVR went along with a lack of significant amount of sunscreen compounds (e.g., mycosporine-like amino acids) in the eggs. The present results demonstrated that gamete viability and embryonic development may be significantly impaired by solar UV radiation in S. granularis, compromising in this way the reproduction of the species. Unless adaptive behavioural reproductive strategies exist, the influence of ambient UV radiation appears as a selective force for population dynamics of broadcast spawners in the shallow benthic Mediterranean environment.     

Counterbalancing effects of maternal mercury exposure during different stages of early ontogeny in American toads

Maternal transfer of environmental contaminants is a disadvantageous parental effect which can have long-lasting implications for offspring fitness. We investigated the effects of mercury (Hg) on the reproductive success of female amphibians and the subsequent effects of maternal transfer on the development of their offspring. American toads (Bufo americanus) maternally transferred Hg to their eggs, and there was a negative relationship between Hg concentrations and the percentage of viable hatchlings produced in clutches. However, when we continued to monitor larvae that successfully hatched, we found 21% greater metamorphic success in larvae from Hg-exposed mothers compared to reference larvae. The negative effect in the embryonic stage and positive effect in the larval stage counterbalanced one another, ultimately resulting in no difference in predicted terrestrial recruitment, regardless of maternal Hg exposure. Our findings demonstrate that maternal effects on survival manifesting at different stages in ontogeny have the potential to produce complicated outcomes.     

Sources of exposure to technologically enhanced natural radiation

The paper presents a review of sources of enhanced natural radiation and their relative importance. Only sources that have been caused by some human technological activity are discussed, even though higher doses may be received by truly natural sources, such as radium rich ground. The distinction between “natural” and “technologically enhanced” sources and the justification of the different sources are discussed.     

Altered developmental timing in early life stages of Antarctic krill (Euphausia superba) exposed to p,p'-DDE

Persistent organic pollutants (POPs) are persistent, toxic and bioaccumulative anthropogenic organic chemicals, capable of undergoing long range environmental transport to remote areas including the Antarctic. p,p′-dichlorodiphenyl dichloroethylene (p,p′-DDE) has been identified as a dominant POP accumulating in Antarctic krill (Euphausia superba), which is a key Southern Ocean species. This study examined the developmental toxicity of p,p′-DDE via aqueous exposure to Antarctic krill larvae. p,p′-DDE exposure was found to stimulate developmental timing in the first three larval stages of Antarctic krill, while extended monitoring of larvae after a five day exposure period had ended, revealed delayed inhibitory responses during development to the fourth larval stage. Stimulatory responses were observed from the lowest p,p′-DDE body residue tested of 10.1 ± 3.0 μmol/kg (3.2 ± 0.95 mg/kg) preserved wet weight, which is comparable to findings for temperate species and an order of magnitude lower than the exposure level found to cause sublethal behavioural effects in Antarctic krill. The delayed responses included increased mortality, which had doubled in the highest p,p′-DDE treatment (95 ± 8.9% mortality at 20 μg/L p,p′-DDE) compared to the solvent control (44 ± 11% mortality) 2 weeks after end of exposure. Development of surviving metanauplius larvae to calyptopis 1 larvae was delayed by 2 days in p,p′-DDE exposed larvae compared with untreated larvae. Finally, the developmental success of surviving p,p′-DDE exposed larvae was reduced by 50 to 75% compared to the solvent control (100% developmental success). The lowest observed effect concentration for all delayed effects was 1 μg/L, the lowest exposure concentration tested. These findings demonstrate the importance of delayed and indirect effects of toxicant exposure. Further, the findings of this study are important for environmental risk assessment of POPs in the Southern Ocean ecosystem and strongly highlight the significance of developmental endpoints for ecotoxicological testing.     

Effects of aluminum and pH on the early life stages of smallmouth bass (Micropterus dolomieui)

The sensitivity of smallmouth bass Micropterus dolomieui to acidified conditions was examined by exposing recently-hatched fish to pH levels ranging from 5.1 to 7.5 and aluminum concentrations ranging from 32 to 1000 μg l⁻¹. The range of pH and aluminum concentrations included those found in the northern part of the species' range. Acute bioassays (96 h) conducted at a pH of 5.1 and aluminum concentrations 180 μgl⁻¹ resulted in total mortality. The LC₅₀ calculated for this species was 130 μg l⁻¹. At pH values of 6.1 and 7.5, mortality was low ( 20%) regardless of aluminum concentrations. A 30-day chronic toxicity test was conducted at three pH levels (low 5.1, intermediate 5.5–5.7 and high 7.3), each with two aluminum concentrations (approx. 0 and 200 μg l⁻¹). Survival was significantly lower in the test at pH 5.1 with aluminum, and at pH 5.7 with aluminum treatments than in the other treatments. Fish in the pH 5.1 without aluminum treatment had intermediate survival, while fish exposed to pH 5.7 without aluminum, pH 7.3 without aluminum and pH 7.3 with aluminum had high, and similar, survival. Sublethal effects on fish exposed to low pH and aluminum included deformities, reduced activity and abnormal swimming behavior. We conclude that the sensitivity of smallmouth bass to low pH and aluminum concentrations corroborates field investigations linking acidification and aluminum mobilization with depletion of smallmouth bass populations.     

Estimation of health effects due to elevated radiation exposure levels in structures

Uranium mill tailings were used as landfill for many years in the United States before the health risk associated with such use was recognized. Occupants of buildings erected on or adjacent to contaminated landfills may experience radiation exposures sufficient to warrant remedial action. Estimates of the cost-effectiveness of the remedial measures may be provided using a combination of occupancy data, appropriate risk coefficients and projected costs. This effort is in support of decisions by the U.S. Department of Energy (DOE) to conduct remedial action at such locations. The methods used in this project, with examples of their application, will be presented in this paper.     

Effects of nitrite concentration and exposure time on sulfide and methane production in sewer systems

Nitrite dosing is a promising technology to prevent sulfide and methane formation in sewers, due to the known inhibitory/toxic effect of nitrite on sulfate-reducing bacteria (SRB) and methanogenic Archaea (MA). The dependency of nitrite-induced inhibition on sulfide and methane producing activities of anaerobic sewer biofilms on nitrite levels and exposure time is investigated using a range of nitrite concentrations (40, 80, 120 mg-N/L) and exposure time up to 24 days. The recovery of these activities after the 24-day nitrite dosage was also monitored for more than two months. The inhibition level was found to be dependent on both nitrite concentration and exposure time, with stronger inhibition observed at higher nitrite concentrations and/or longer exposure time. However, the time required for achieving 50% recovery of both sulfate-reducing and methanogenic activities after the cessation of nitrite dosage only marginally depended on nitrite concentration. Model-based analysis of the recovery data showed that the recovery was likely due to the regrowth of SRB and methanogens. The lab studies and mathematical analysis supported the development of an intermittent dosing strategy, which was tested in a 1-km long rising main sewer. The field trial confirmed that intermittent dosing of nitrite can effectively reduce/prevent the formation of both sulfide and methane.     

Drama,play-making and ambiguity in the early stages of design

A number of techniques from drama and theatre have been appropriated for use in design to stage performances with and about users. In this paper, we address how drama and play-making can benefit design at moments other than staging. Building on the work of drama-in-education scholar Gavin Bolton, we articulate design-relevant learning qualities of using drama in design. We recognise that significant moments of learning about human conditions and technology take place in making and managing staged performances. We point to how significant but often implicit design decisions are taken in the making of a play and study their conditioning effect on the continuation of a design process. The focus of our inquiry is on the development of an oblique angle and its indirect directive qualities as a pre-set framing for using drama in design as well as an emergent framing that follows from using drama in design.     

Combined exposure to cyanobacterial biomass, lead and the Newcastle virus enhances avian toxicity

Under environmental conditions, wild birds can be exposed to multiple stressors including natural toxins, anthropogenic pollutants and infectious agents at the same time.This experimental study was successful in testing the hypothesis that adverse effects of cyanotoxins, heavy metals and a non-pathogenic immunological challenge combine to enhance avian toxicity. Mortality occurred in combined exposures to naturally occurring cyanobacterial biomass and lead shots, lead shots and Newcastle vaccination as well as in single lead shot exposure. Mostly acute effects around day 10 were observed. On day 30 of exposure, there were no differences in the liver accumulation of lead in single and combined exposure groups. Interestingly, liver microcystin levels were elevated in birds co-exposed to cyanobacterial biomass together with lead or lead and the Newcastle virus. Significant differences in body weights between all Pb-exposed and Pb-non-exposed birds were found on days 10 and 20. Single exposure to cyanobacterial biomass resulted in hepatic vacuolar dystrophy, whereas co-exposure with lead led to more severe granular dystrophy. Haematological changes were associated with lead exposure, in particular. Biochemical analysis revealed a decrease in glucose and an increase in lactate dehydrogenase in single and combined cyanobacterial and lead exposures, which also showed a decreased antibody response to vaccination.The combined exposure of experimental birds to sub-lethal doses of individual stressors is ecologically realistic. It brings together new pieces of knowledge on avian health. In light of this study, investigators of wild bird die-offs should be circumspect when evaluating findings of low concentrations of contaminants that would not result in mortality on a separate basis. As such it has implications for wildlife biologists, veterinarians and conservationists of avian biodiversity.     

Combined heat transfer of radiation and conduction in stacked radiation shields for vacuum insulation panels

Combined heat transfer of radiation and conduction considering depthwise conduction phenomena in stacked radiation shields for vacuum insulation panels (VIPs) having an artificial core structure is investigated. The purpose of this study is to establish a reliable analysis method for actual shield design. A one-dimensional problem is first analyzed. Actual two-dimensional problem is simplified for theoretical modeling and the depthwise conduction around contact spot is further considered. Test radiation shields are manufactured using press molding process with SUS 304 (AISI grade 304) plates, since this sample depicts the parametric effects well. Measurement of the effective thermal conductivity is performed with a VGHP apparatus. While various analytical and numerical models predict the effective thermal conductivity in the case of this specific specimen within about 40% error, the combined heat transfer analysis considering depthewise conduction phenomena is accurate within 10% error and it is thus recommended in estimating the effective thermal conductivity of stacked radiation shields.     

Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on early life stages of the pike (Esox lucius L.)

Freshly fertilized pike eggs were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations of 0.1, 1.0 and 10 ppt (ng/liter) for 96 hours. At all concentrations examined egg development was retarded by 23%, and the growth of fry was also significantly retarded for a long period after exposure. A dose-related mortality was observed. Highest mortality rates occurred during resorption of the yolk and reached almost 100 percent at a concentration of 10 ppt. Death was preceded by development of severe generalized edemas.Histopathologically edemas and hemorrhages were observed, together with alterations of bloodvessel walls. In the liver, two stages of pathological changes were distinguished. The first was characterized by a dilation of sinusoids and a slight swelling of hepatocyte nuclei; in the second stage the nuclei were enlarged up to twice the normal diameter. Hepatocytes were degenerated and varied in size and shape and liver architecture was a almost completely lost.     

Legal implications of exposure to enhanced natural radiation in the context of the euratom directives

The Euratom Directives apply to “enhanced natural radiation” as far as these radiation concerns operations with respect to substances not to “be disregarded as far as radiation protection is concerned” and accordingly it is a subject of the policy of the Member States. In this case application of “enhanced natural radiation” should be subjected to the basic principles of the 1980 Directive. Measures to be taken in national and international context are analysed.     

A framework for a limitation policy of the population exposure to natural radiation in Belgium

The conclusions are put forward that are adopted by a discussion group preparing the framework for a limitation policy of the radon problem in Belgium. Existing and future situations are treated in a coherent policy that is adequate for practical implementation. An action level is defined, together with a hierachy of levels for new constructions. The decision logic for the acceptance of building materials is explained.     

Method and simulation program informed decisions in the early stages of building design

The early stages of building design include a number of decisions which have a strong influence on the performance of the building throughout the rest of the process. It is therefore important that designers are aware of the consequences of these design decisions. This paper presents a method for making informed decisions in the early stages of building design to fulfil performance requirements with regard to energy consumption and indoor environment. The method is operationalised in a program that utilises a simple simulation program to make performance predictions of user-defined parameter variations. The program then presents the output in a way that enables designers to make informed decisions. The method and the program reduce the need for design iterations, reducing time consumption and construction costs, to obtain the intended energy performance and indoor environment.     

Effects of oral exposure to polychlorinated biphenyls (PCBs) on the development and metamorphosis of two amphibian species (Xenopus laevis and Rana temporaria)

This study examined the effects of polychlorinated biphenyls (PCBs) on development of families of amphibians using the African clawed frog (Xenopus laevis) and the European common frog (Rana temporaria). Amphibians were orally exposed to the technical PCB-mixture Clophen A50 or to the non-ortho-3,3',4,4',5-CB congener (PCB 126) either for a 10-day period or until metamorphosis. Occurrence and rate of malformations, mortality, period until metamorphosis and thyroid hormone levels were measured. Mortality increased in a dose-dependent manner, as did the rates of malformation. Time until metamorphic transformation was prolonged and the weight of froglets was increased. Although not statistically significant, thyroid hormone levels were also lowered. PHAHs such as PCBs may affect important aspects of amphibian fitness and may influence amphibian reproductive success.     

Project governance and path creation in the early stages of Finnish nuclear power projects

In this paper we focus on the early stages of projects and their governance implications by investigating the introduction of nuclear power in Finland. We look at the early stages through the lens of path dependence and illustrate how early decisions may lead the project down the wrong path. However, we also develop a path creation perspective in order to develop an understanding of how the project actors in our case deliberately navigated in the cold war atmosphere to avoid a budding lock-in. This historical study highlights the importance of building relationships between key actors and securing their commitment during the early stages of a project. We argue that strong relationships and commitment open up opportunities for creating alternative paths during project appraisal, increase the array of available governance mechanisms, and hence lay the foundations for the final governance structure of the project execution phase.     

Heat transfer modelling on windows and glazing under the exposure of solar radiation

Due to the effect of solar radiation on windows and glazing system the evaluation of heat flow is of primary importance in modeling the thermal performance within building interiors to account thermal comfort and overall energy consumption of a building. In this context the optical properties of window glazing are measured to determine the percentage absorption of incident solar radiation. An experimental study was performed in a room to measure the glazing surface temperature due to the global radiation on it. The corresponding window plane global radiation and horizontal global radiation were measured outside for simulation. Mathematical models have been developed to simulate the window plane solar radiation and corresponding glazing surface temperature aiming at validating the measured values. The thermal model is concerned with laminar heat transfer for natural and forced convection process according to the ambient conditions. The estimated errors between experimental and simulated values of window plane radiation and glazing temperature are shown to be within ±5%. Using the developed thermal model the heat flow inside the room through windows is determined. Thus overall heat transfer coefficient of glazing (U-factor) and the Solar Heat Gain (SHG) of building interior have been predicted from the simulation.     

Service life prediction and environmental exposure conditions of timber claddings in South Chile

ABSTRACT

In this research, a methodology for the service life prediction of external timber claddings is proposed and discussed. The proposed model is based on the visual assessment of 110 timber claddings, subjected to real exposure conditions. The claddings under analysis belong to 80 buildings, with and without heritage characteristics, located in Valdivia and Niebla, South Chile. Timber claddings are a relevant symbol of the local identity of this region, portraying a complexity of ideas and cultural values, inherited by Spanish and German colonizations. The main anomalies that can occur in these claddings are translated into a numerical index, which describes the overall degradation condition of a timber façade. This work also discusses the influence of the local climate and environmental exposure conditions, considering biotic factors (fungi, insects, moulds, algae and bacteria) and abiotic factors (water, solar radiation, amplitude of temperature, physical damage) in the evolution of the degradation condition of timber claddings. The scarcity of funds in Chile to maintain and preserve the constructions and its components needs a more rational approach to decision-making concerning inspection, maintenance and rehabilitation procedures. The expeditious tool proposed in this paper may help in providing the basis for rational decision-making by professionals in practice.