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本文研究了18α、18β-甘草酸单体抗酒精性肝损伤作用。SD大鼠随机分为4组,分别为正常组,模型组,18α-甘草酸组,18β-甘草酸组。除正常组外,各组灌胃给予40%酒精,给药组分别灌胃给予18α-甘草酸和18β-甘草酸,每天给药一次,持续4周。于第4周,进行口服葡萄糖耐量实验(OGTT)。给药结束后,将所有大鼠麻醉处死,检测血清和肝脏中的生化指标,并进行肝组织病理学观察。与模型组相比,18α-甘草酸和18β-甘草酸单体对肝功能及抗氧化指标均有显著性改善作用;18β-甘草酸对糖、脂质、蛋白质的代谢具有显著性调控作用;病理切片结果显示,18α-甘草酸和18β-甘草酸均对肝细胞具有较好的保护作用。因此,18α-甘草酸和18β-甘草酸单体对酒精性肝损伤均有较好的保护作用,其中18β-甘草酸在调节糖、脂质及蛋白质代谢方面的作用优于18α-甘草酸。 相似文献
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研究利用多级逆流技术提取甘草酸的新方法.采用正交试验考察提取温度、提取时间、级数和液固比对提取效率的影响,确定多级逆流提取甘草酸的最佳工艺条件;在优选出的最佳工艺条件下,考察了提取溶剂对提取率的影响,并与室温冷浸法、超声波法、索氏提取法和微波提取法做了比较.结果表明:多级逆流提取甘草酸的最佳条件为提取温度70 ℃,单级... 相似文献
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由中国科学院过程工程研究所绿色过程与工程重点实验室攻关完成的课题一“溶剂萃取法分离提纯甘草中甘草酸”研究通过鉴定。该项目旨在提高我国甘草资源利用价值。课题组通过对甘草中有效成分的分离研究,并对现有工艺条件和传统方法的萃取过程进行了大胆的创新和实验,开发出一条通过溶剂萃取法对甘草酸进行分离提纯的新型工艺路线。新工艺改变了传统方法提取甘草中的甘草酸回收率低、 相似文献
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甘草有效成分分离及其对自由基的清除能力 总被引:19,自引:0,他引:19
对甘草水提取物及提取的甘草酸的羟自由基清除能力进行了评价,结果显示甘草水提取物对羟自由基清除能力高于提纯的甘草酸溶液,并且二者的羟自由基清除能力远远大于同浓度的Vc溶液。 相似文献
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Glycyrrhizin is the main pharmacologically active component in licorice. Long term consumption of higher amounts of glycyrrhizin results in serious side effects (pseudoaldosteronism) caused by the glycyrrhizin metabolite 3β-monoglucuronyl-18β-glycyrrhetinic acid (3-MGA). The aim of this study was to determine urine excretion profile of 3-MGA. Six healthy volunteers ingested 600 mg of glycyrrhizin. In the first experimental period, glycyrrhizin was ingested in the morning and in the second experimental period, which followed after 2 weeks, glycyrrhizin was ingested in the evening. Results showed that the amount of excreted 18-MGA in urine was 1425.9-3147.8 μg, which corresponds to 0.31-0.67% of the ingested dose. Maximum elimination rates were 31.52-209.47 μg/h. There were no significant differences in the amount of excreted 3-MGA as well as in elimination rates of 3-MGA when glycyrrhizin was ingested in the morning or evening, respectively. These results provide some important clinical data on glycyrrhizin metabolism. 相似文献
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The glycyrrhizin contents of 42 samples of liquorice-containing confectionery, health products and raw materials have been determined by a standard (AOAC) HPLC technique. Confectionery levels ranged between 0.26 and 7.9 mg g-1, whilst contents in health products were 0.30-47.1 mg g-1, the highest values being measured for throat pearls. Six geographically diverse samples of liquorice root contained similar (22.2-32.3 mg g-1) glycyrrhizin contents. Highest levels of glycyrrhizin were found in liquorice block (44-98 mg g-1) and extract powder (79-113 mg g-1). These analyses enable a mean daily intake of glycyrrhizin to be calculated for the UK. The figure (1 mg) is lower than those reported for the US and Belgium (3 and 5 mg, respectively). The significance of the levels of glycyrrhizin in UK confectionery, and the estimated daily exposure thereto, is discussed in the context of existing data on liquorice-induced toxicity. 相似文献
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为探讨甘草甜素调节U14 宫颈癌小鼠免疫功能及诱导凋亡作用,将U14宫颈癌小鼠随机分为模型组、甘草甜素低、中、高剂量组(25、50、100 mg/kg)及环磷酰胺组(25 mg/kg),另设正常组,每组10只;灌胃给药,1次/d,共21 d。测量各组小鼠瘤体积(最大直径)、瘤质量,计算胸腺指数、脾脏指数,TUNEL法检测细胞凋亡指数,实时定量PCR法检测Caspase-3、Bax及Bcl-2 mRNA表达,Western Blot法检测p-PI3K、p-AKT蛋白表达等指标。结果发现,与模型组比较,甘草甜素低、中、高剂量组小鼠瘤体积(最大直径)及瘤质量显著降低(P<0.05或P<0.01),抑瘤率分别为15.68%、25.41%和38.38%;与模型组比较,甘草甜素低、中、高剂量组小鼠胸腺指数、脾脏指数、IL-2、IFN-γ、TNF-α含量、凋亡指数、Caspase-3及Bax mRNA表达显著增加(P<0.05或P<0.01),而Bcl-2 mRNA及p-PI3K、p-AKT蛋白表达显著降低(P<0.05或P<0.01)。上述结果表明,甘草甜素具有抑制U14宫颈癌小鼠肿瘤生长的作用,该作用与改善免疫功能及诱导凋亡作用有关。 相似文献
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以酶法改变甘草苷糖醛酸基提高其甜度为目的,对新分离筛选的甘M-2和甘M-6霉菌产的β-葡萄糖醛酸苷酶进行了分离提纯和酶学方面的研究。结果表明,两株菌产的β-葡萄糖醛酸苷酶被60%饱和硫酸铵沉淀较好,经DEAE-CeluoseDE52离子交换层析柱梯度洗脱,得到纯酶,提纯倍数分别为10.67和6.15倍,收率分别为33.0%和24.2%,其中甘M-2菌产β-葡萄糖醛酸苷酶只能将甘草苷水解成甘草次酸(GA);甘M-6菌产β-葡萄糖醛酸苷酶水解甘草苷主要变成甜度很高的单葡萄糖醛酸基甘草苷(GAMG);两种酶在SDS-聚丙烯酰胺凝胶电泳都得到电泳单点,其酶蛋白相对分子质量分别为60000和42000;酶反应最适pH分别为5.0和6.0,最适温度均为40℃,均在pH4.0~8.0和20~70℃范围内相对稳定。 相似文献
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目的观察单磷酸阿糖腺苷联合复方甘草酸苷治疗带状疱疹的疗效和安全性。方法随机将60例患者分为2组,治疗组用单磷酸阿糖腺苷联合复方甘草酸苷,对照组只用单磷酸阿糖腺苷,比较2组止疱、干涸、止痛时间及后遗神经痛发生率。结果治疗组和对照组止疱时间分别为(2.53±0.39)d和(2.84±0.60)d,疼痛减轻时间(2.71±0.30)d和(3.27±0.48)d,止痛时间为(3.91±0.42)d和(5.11±1.08)d,皮损痊愈时间为(7.33±0.33)d和(9.38±0.43)d,2组有效率分别是93.3%和70.0%,差异有统计学意义(P〈0.05,P〈0.01)。结论单磷酸阿糖腺苷联合复方甘草酸苷治疗带状疱疹疗效好。 相似文献
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以含氨0.6%的65%乙醇溶液为浸提溶剂,探讨影响微波辅助提取甘草酸的主要因素,最终确定微波辅助提取甘草酸的最佳工艺条件:固液比(g/mL)为1:9,微波处理时间4.0 min,微波功率为中低火,提取次数3次.在此工艺条件下,甘草酸的得率为12.59%. 相似文献
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Isolation and identification of main constituents in an enzymatically hydrolysed licorice extract sweetener 总被引:1,自引:0,他引:1
Licorice extract including glycyrrhizin as the major component is used as a safe sweetener in many countries. Enzymatically hydrolysed licorice extract (EHLE) is also used as a sweetener in Japan. In the present paper, the constituents of EHLE were investigated as a part of the studies for evaluating its quality and safety as a food additive. Three oleanane-type monoglycosides along with glycyrrhizin (1) eand 3-O-[beta-D-glucuronopyranosyl-(1-->2)-beta-D-glucuronopyranosyl]-18beta-liquiritic acid (2) were isolated from EHLE. The structures of the three compounds have been determined to be 3-O-beta-D-glucuronopyranosyl-24-hydroxy-18beta-glycyrrhetinic acid (3), 3-O-beta-D-glucur-onopyranosyl-18beta-glycyrrhetinic acid (4) and 3-O-beta-D-glucuronopyranosyl-18beta-liquiritic acid (5) based on MS and NMR. Compound 4 was the monoglycosylated derivactive of glycyrrhizin (1). Compounds 3 amd 5 are the monoglycosylated derivatives of the minor constituents in licorice extract. They were first isolated from EHLE, and compound 5 was a new compound. 相似文献
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Yang Yongan Wei Yuangang Guo Xiaonan Qi Pengfei Zhu Hailiang Tang Wenjian 《Food science and biotechnology》2019,28(4):1187-1193
Food Science and Biotechnology - Glycyrrhetic acid monoglucuronide (GAM) is obtained from the natural sweetener glycyrrhizin through enzymolysis. Its sweetness concentration–response... 相似文献